MD07G1201900.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002776
Physical Location & Seq
Forward (+)
28005681 .. 28013321
7641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1201900.v1.1.491

Sequence Viewer

Length: 3075 bp
ATGGTCACCGATACTTATTCGACGATGTCCGACATGTCTATGAGATCGATGTTGAAAAGCGGCGAGTACAATGAAGATTTGAGCATGTTGATTCGCGAACAGAGGCGCCAGCACGAAGCAAGTGAGCGGGAGAAGGAACTGAATATATACAGAAGTGGGTCGGCGCCGCCTACCGTGGAAGGTTCGTTAAACGCGGTCGGTGGCTTGTTTGAGGCGTCCTCGCTGTCGGGTTTCATGAAAAACGACAGCAAAGGGTTTGCAACTGAGGAGGAGCTTCGCGCCGATCCAGCTTATGTGAATTACTATTATTCCAATGTCAATCTCAATCCGCGGCTTCCGCCGCCGCTCCTTTCAAAGGAGGACTGGCGTTTTGCGCAGCGGTTGCAAGGCGGAGGTGGAGTTTCGGCGGTTGGAGATAGGAGGATAGGTGGCAGGAGTGGTGGTGAAGGGTCACTTTTTTCTGTGCAGCCGGGGATTGGAGGGAATGAGGAGAATGGTGTGGCGGCGAGGAAGGGTGCTGCAGAGTGGGGCGGTGATGGGTTGATTGGGTTACCGGGGTTGGGACTTGGGAGTAGGCGGAAGAGTATTGCGGAGATTATTCAGGATGATATACATAATACAAATGTCTCAAGGCACCCTTCTCGTCCAGCTAGCCAGAATGCGTTTGATGATGGTGTTGATGCTTCAGATACCCAGTTTGCCCATTTACAGGGAATGTCAGCTGTTCAGAACGGGGGTTCATCCAGTTCTCATACATATGCTTCTGCTCTGGGTGCTTCATTGTTGAGAAGCAATACACCAGACCCTCAGCTTGTAGCTAGGGCTCCTAGTCCTCGAATTCCACCAGTTGGTGGAGGAAGGACTTCCTCCATGGATAAAAAAGTTGGCAATGGTCAAAACTCATTCAATGGTGCCTCGCCCAAAGTGAATGAGTCTGCAGATCTGGCAGCTGCTTTATCTGGTATGAACCTGTCAACTAATGGTAGGATGGATGAGGAGAATCATGCACGATCACAAATTGATAATCACCATAACCGTTTTGATATGCAAGGTGATCGGAACCATAATAAACAAAATTCGTACTTGAATAAGTCTGATTCTGGGAGTTTTCATCTGCATTCTGCTTCTCAATCGTCTAACAAGCCTTATCAGAATATGGCAAGAGGCAGTGGATTTGGCAGGGATCTGAATAGCCCTTCATATATGTCCGATGATCTAGTTGATATTAATAATCCTGCTGTATCTTCTGCGAACTCATACTTAAGAGGACCTGTGCCAACTCTTCATGGCCGAGGAAGCTCACACTCCCACTATCAGAATGTAGACAATACCTCATTTCCGAACTACGGCTATTCTGGTAGTCCTTTGTCTCCATCCATGATGGGTAGTCCCCTTGGTAATGGTAGTTTACCACCCTTATTTGAAAATGCTGCTGCTGCATCTGCAATGGGTGGCTTGGACTCCGGAGCATTTGGAGGTATGTCTCTAGGACCAAATCTGTTGGCAGCAGCCGCTGAATTGCAGAACATTAGGGTTGGAAATCATGGTACTGGGAGTGCTCTTCAGGTTCCCATGATGGACCCATTGTATGTTCAGTACTTGAGATCAAATGAATATGCAGCTGCACAGCTTGCAGCCCTTAATGACCCCACAAAAGATAGGGAAGGCATGGGTAGTATGCACATGGATTTACTTGGTTTGCAAAAAGCTTATTTGGGGCAATTCCTCACACCCCAAAAATCACAATTTGGTGCTCCCTACGTTGGCAAGTCTGCTAGCTTGAATAACGGTTATTATGGAAATCCAGCATATGGGCATGGGATGTCTTATTCTGGAACACCATTGGGTGGTCCCCTTCTTCCAAACTCTCCTGTTGGACCTGGTAGTCCGGCCAGGCATGGTGAGCGGAACTTGCGCTTTTCTTCTGGGTTGAGGAACATGGGTGGAGGTCTCGTGGGAGCTTGGCACTCCGAAACTGGTGGAAATTTGGATGAAAGTTTTGCGTCCTCTTTATTAGATGAGTTCAAAAGCAATAAGACTAGATGTTTTGAACTTTCAGAGATTGCAGGGCATGTTGTTGAGTTCAGTGCCGACCAGTATGGAAGTCGGTTTATTCAACAGAAACTTGAAACTGCCACAATTGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCGCAAGCCCTATCTCTTATGACTGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTTGAGCATGGAACTCCAGCACAAATAAGAGAACTTGCGGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAATTTGTTAAGCTGGACCAGCAGACTAAAATGGTAGCCGAACTGGATGGTCATGTTATGCGTTGTGTTCGTGATCAAAATGGCAACCATGTTGTCCAGAAGTGTATTGAATGTGTACCTGAAGATGCCATCCAGTTTGTTGTTTCAACTTTTTATGATCAAGTTGTGACACTCTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAGTTTTGGAGCACTGCCATGACCCCAAAACGCAGCAAATAATGATGGATGAGATTTTGCAGTCCGTTTGCATTTTGGCACAAGACCAGTATGGAAATTATGTCGTGCAGCATGTGCTGGAACATGGAAAGCCACATGAACGGTCATGTATAATTAAGGAACTAACTGGGCAGATTGTTCAAATGAGCCAGCAAAAGTTTGCGTCAAATGTTATAGAAAAGTGTTTAAGTTTTGGAACTCTTGCTGAACGCCAGGCCCTTGTAACTGAGATGCTTGGTACCACTGATGAAAATGAGCCCCTTCAGGCGATGATGAAGGATCAGTTCGCAAACTATGTTGTACAGAAAGTGCTGGAAACTTGTGACGATCAGCAACTTGAACTAATCCTTAACCGAATTAAGGTTCATCTGAATGCTCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCACGTGTAGAGAAACTTGTTGCTGCTGGGGAGAGGAGGATTAGCATTCTGGCTCCCCAGGCATCTGCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1025

Amino Acids

111.04

Weight (kDa)

6.03

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 274 - 686 8.8e-151 Nucleic acid binding protein NABP
PUF PF00806 691 - 725 2.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 728 - 760 8.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 763 - 796 5e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 796 - 871 1e-05 NOP9-like PUF repeat domain
PUF PF00806 835 - 870 5.1e-13 Pumilio-family RNA binding repeat
PUF PF00806 873 - 906 8.7e-10 Pumilio-family RNA binding repeat
PUF PF00806 908 - 941 2.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 954 - 983 9.3e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1884
Acc16I TGCGCA 1 cut(s) 375
Acc65I GGTACC 1 cut(s) 2828
AccB1I GGYRCC 5 cut(s) 105, 163, 633, 911, 2828
AccB7I CCANNNNNTGG 4 cut(s) 848, 851, 1811, 1847
AccBSI CCGCTC 3 cut(s) 127, 346, 1906
AccI GTMKAC 2 cut(s) 1323, 2978
AccII CGCG 4 cut(s) 96, 194, 279, 331
AccIII TCCGGA 1 cut(s) 1463
AclWI GGATC 4 cut(s) 278, 1191, 2323, 2877
AcoI YGGCCR 2 cut(s) 1288, 1890
AcsI RAATTY 4 cut(s) 837, 1075, 1984, 2345
AcuI CTGAAG 5 cut(s) 669, 1547, 2484, 2837, 2990
AcvI CACGTG 1 cut(s) 3005
AcyI GRCGYC 3 cut(s) 106, 164, 215
AfaI GTAC 7 cut(s) 68, 1082, 1549, 1598, 2460, 2830, 2892
AflII CTTAAG 1 cut(s) 1261
AflIII ACRYGT 2 cut(s) 33, 3004
AjnI CCWGG 4 cut(s) 1879, 1892, 2802, 3057
Alw21I GWGCWC 3 cut(s) 1561, 1756, 2565
Alw26I GTCTC 4 cut(s) 631, 1374, 1488, 1955
AlwI GGATC 4 cut(s) 278, 1191, 2323, 2877
AlwNI CAGNNNCTG 1 cut(s) 1514
Aor13HI TCCGGA 1 cut(s) 1463
AoxI GGCC 3 cut(s) 1288, 1890, 2805
ApoI RAATTY 4 cut(s) 837, 1075, 1984, 2345
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AseI ATTAAT 1 cut(s) 1227
Asp700I GAANNNNTTC 1 cut(s) 862
Asp718I GGTACC 1 cut(s) 2828
AspLEI GCGC 5 cut(s) 108, 166, 281, 376, 1917
AspS9I GGNCC 8 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359, 2806
AsuC2I CCSGG 2 cut(s) 471, 555
AsuHPI GGTGA 5 cut(s) 455, 545, 1019, 1064, 1913
AsuNHI GCTAGC 2 cut(s) 650, 1775
AvaII GGWCC 7 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359
BanI GGYRCC 5 cut(s) 105, 163, 633, 911, 2828
BanII GRGCYC 2 cut(s) 826, 2850
BauI CACGAG 1 cut(s) 1952
BbrPI CACGTG 1 cut(s) 3005
Bbv12I GWGCWC 3 cut(s) 1561, 1756, 2565
BbvCI CCTCAGC 1 cut(s) 807
BccI CCATC 9 cut(s) 530, 665, 982, 1375, 1381, 1570, 2384, 2480, 2590
BceAI ACGGC 1 cut(s) 1363
BcgI CGANNNNNNTGC 4 cut(s) 623, 657, 1037, 1071
BciT130I CCWGG 4 cut(s) 1881, 1894, 2804, 3059
BclI TGATCA 2 cut(s) 2416, 2500
BcnI CCSGG 2 cut(s) 471, 555
BcoDI GTCTC 4 cut(s) 631, 1374, 1488, 1955
BfaI CTAG 7 cut(s) 651, 819, 828, 1217, 1487, 1776, 2040
BfmI CTRYAG 3 cut(s) 519, 936, 2340
BfoI RGCGCY 2 cut(s) 109, 167
BfrI CTTAAG 1 cut(s) 1261
BglII AGATCT 1 cut(s) 940
BmcAI AGTACT 1 cut(s) 1598
Bme1390I CCNGG 6 cut(s) 471, 555, 1881, 1894, 2804, 3059
Bme18I GGWCC 7 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359
BmgT120I GGNCC 8 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359, 2806
BmrFI CCNGG 6 cut(s) 471, 555, 1881, 1894, 2804, 3059
BmrI ACTGGG 4 cut(s) 688, 1560, 2292, 2727
BmsI GCATC 5 cut(s) 670, 1448, 2458, 2811, 3071
BmtI GCTAGC 2 cut(s) 654, 1779
BmuI ACTGGG 4 cut(s) 688, 1560, 2292, 2727
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 1 cut(s) 2232
Bpu10I CCTNAGC 2 cut(s) 807, 2300
BpuEI CTTGAG 2 cut(s) 613, 1621
BpuMI CCSGG 2 cut(s) 471, 555
Bsa29I ATCGAT 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 3005
BsaHI GRCGYC 3 cut(s) 106, 164, 215
BsaI GGTCTC 1 cut(s) 1955
BsaJI CCNNGG 8 cut(s) 174, 329, 470, 554, 870, 1291, 1393, 3057
BsaWI WCCGGW 1 cut(s) 1463
BsaXI ACNNNNNCTCC 8 cut(s) 384, 412, 414, 427, 442, 457, 1938, 1968
Bse3DI GCAATG 2 cut(s) 895, 1452
BseAI TCCGGA 1 cut(s) 1463
BseBI CCWGG 4 cut(s) 1881, 1894, 2804, 3059
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 8 cut(s) 174, 329, 470, 554, 870, 1291, 1393, 3057
BseMI GCAATG 2 cut(s) 895, 1452
BseMII CTCAG 3 cut(s) 255, 821, 2808
BseRI GAGGAG 5 cut(s) 281, 284, 503, 1010, 3049
BseYI CCCAGC 1 cut(s) 3026
BsgI GTGCAG 3 cut(s) 485, 1608, 2678
Bsh1236I CGCG 4 cut(s) 96, 194, 279, 331
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 3 cut(s) 1290, 1892, 2807
BshNI GGYRCC 5 cut(s) 105, 163, 633, 911, 2828
BshVI ATCGAT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 3 cut(s) 1561, 1756, 2565
BsiSI CCGG 4 cut(s) 470, 554, 1464, 1889
BslFI GGGAC 3 cut(s) 576, 1374, 1836
BsmAI GTCTC 4 cut(s) 631, 1374, 1488, 1955
BsmFI GGGAC 3 cut(s) 576, 1374, 1836
BsmI GAATGC 4 cut(s) 664, 1117, 2968, 3045
BsnI GGCC 3 cut(s) 1290, 1892, 2807
Bso31I GGTCTC 1 cut(s) 1955
Bsp1286I GDGCHC 5 cut(s) 826, 1561, 1756, 2565, 2850
Bsp13I TCCGGA 1 cut(s) 1463
Bsp1407I TGTACA 1 cut(s) 2890
Bsp19I CCATGG 1 cut(s) 870
Bsp68I TCGCGA 1 cut(s) 96
BspANI GGCC 3 cut(s) 1290, 1892, 2807
BspCNI CTCAG 3 cut(s) 256, 820, 2809
BspDI ATCGAT 1 cut(s) 47
BspEI TCCGGA 1 cut(s) 1463
BspFNI CGCG 4 cut(s) 96, 194, 279, 331
BspHI TCATGA 1 cut(s) 234
BspMAI CTGCAG 2 cut(s) 523, 940
BspOI GCTAGC 2 cut(s) 654, 1779
BspPI GGATC 4 cut(s) 278, 1191, 2323, 2877
BspQI GCTCTTC 1 cut(s) 1566
BspT107I GGYRCC 5 cut(s) 105, 163, 633, 911, 2828
BspTI CTTAAG 1 cut(s) 1261
BspTNI GGTCTC 1 cut(s) 1955
BsrBI CCGCTC 3 cut(s) 127, 346, 1906
BsrDI GCAATG 2 cut(s) 895, 1452
BsrGI TGTACA 1 cut(s) 2890
BssECI CCNNGG 8 cut(s) 174, 329, 470, 554, 870, 1291, 1393, 3057
BssNAI GTATAC 1 cut(s) 2979
BssNI GRCGYC 3 cut(s) 106, 164, 215
BssSI CACGAG 1 cut(s) 1952
BssT1I CCWWGG 2 cut(s) 870, 1393
Bst1107I GTATAC 1 cut(s) 2979
Bst2BI CACGAG 1 cut(s) 1952
Bst2UI CCWGG 4 cut(s) 1881, 1894, 2804, 3059
Bst4CI ACNGT 4 cut(s) 175, 1037, 1790, 2694
Bst6I CTCTTC 3 cut(s) 575, 1287, 1566
BstACI GRCGYC 3 cut(s) 106, 164, 215
BstAFI CTTAAG 1 cut(s) 1261
BstAPI GCANNNNNTGC 3 cut(s) 382, 1631, 2665
BstAUI TGTACA 1 cut(s) 2890
BstBAI YACGTR 1 cut(s) 3005
BstC8I GCNNGC 6 cut(s) 110, 652, 1632, 1777, 2181, 2741
BstDEI CTNAG 5 cut(s) 264, 807, 2300, 2817, 3072
BstDSI CCRYGG 3 cut(s) 174, 329, 870
BstEII GGTNACC 2 cut(s) 4, 549
BstENI CCTNNNNNAGG 1 cut(s) 353
BstFNI CGCG 4 cut(s) 96, 194, 279, 331
BstH2I RGCGCY 2 cut(s) 109, 167
BstHHI GCGC 5 cut(s) 108, 166, 281, 376, 1917
BstMAI GTCTC 4 cut(s) 631, 1374, 1488, 1955
BstMCI CGRYCG 1 cut(s) 198
BstNI CCWGG 4 cut(s) 1881, 1894, 2804, 3059
BstNSI RCATGY 5 cut(s) 37, 88, 2075, 2291, 2666
BstPI GGTNACC 2 cut(s) 4, 549
BstSCI CCNGG 6 cut(s) 469, 553, 1879, 1892, 2802, 3057
BstSFI CTRYAG 3 cut(s) 519, 936, 2340
BstUI CGCG 4 cut(s) 96, 194, 279, 331
BstX2I RGATCY 2 cut(s) 940, 1183
BstXI CCANNNNNNTGG 1 cut(s) 2282
BstYI RGATCY 2 cut(s) 940, 1183
BstZ17I GTATAC 1 cut(s) 2979
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 3 cut(s) 1290, 1892, 2807
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 3 cut(s) 174, 329, 870
BtgZI GCGATG 1 cut(s) 2873
BtsI GCAGTG 2 cut(s) 1174, 2563
BtsIMutI CAGTG 5 cut(s) 1174, 2092, 2280, 2563, 2832
BtuMI TCGCGA 1 cut(s) 96
Cac8I GCNNGC 6 cut(s) 110, 652, 1632, 1777, 2181, 2741
CaiI CAGNNNCTG 1 cut(s) 1514
CciI TCATGA 1 cut(s) 234
CfoI GCGC 5 cut(s) 108, 166, 281, 376, 1917
Cfr13I GGNCC 8 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359, 2806
Cfr42I CCGCGG 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 47
CseI GACGC 3 cut(s) 204, 1992, 2742
CsiI ACCWGGT 1 cut(s) 1879
Csp6I GTAC 7 cut(s) 67, 1081, 1548, 1597, 2459, 2829, 2891
CviQI GTAC 7 cut(s) 67, 1081, 1548, 1597, 2459, 2829, 2891
DdeI CTNAG 5 cut(s) 264, 807, 2300, 2817, 3072
DinI GGCGCC 2 cut(s) 107, 165
DrdI GACNNNNNNGTC 1 cut(s) 1884
DseDI GACNNNNNNGTC 1 cut(s) 1884
EaeI YGGCCR 2 cut(s) 1288, 1890
Eam1104I CTCTTC 3 cut(s) 575, 1287, 1566
EarI CTCTTC 3 cut(s) 575, 1287, 1566
EciI GGCGGA 3 cut(s) 327, 405, 592
Eco130I CCWWGG 2 cut(s) 870, 1393
Eco24I GRGCYC 2 cut(s) 826, 2850
Eco31I GGTCTC 1 cut(s) 1955
Eco47I GGWCC 7 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359
Eco57I CTGAAG 5 cut(s) 669, 1547, 2484, 2837, 2990
Eco72I CACGTG 1 cut(s) 3005
Eco91I GGTNACC 2 cut(s) 4, 549
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 1268, 2806
EcoO65I GGTNACC 2 cut(s) 4, 549
EcoRI GAATTC 1 cut(s) 837
EcoRII CCWGG 4 cut(s) 1879, 1892, 2802, 3057
EcoT14I CCWWGG 2 cut(s) 870, 1393
EcoT38I GRGCYC 2 cut(s) 826, 2850
EgeI GGCGCC 2 cut(s) 107, 165
EheI GGCGCC 2 cut(s) 107, 165
ErhI CCWWGG 2 cut(s) 870, 1393
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 622, 654
FaqI GGGAC 3 cut(s) 576, 1374, 1836
FauI CCCGC 1 cut(s) 120
FauNDI CATATG 3 cut(s) 757, 1810, 2530
FbaI TGATCA 2 cut(s) 2416, 2500
FblI GTMKAC 2 cut(s) 1323, 2978
FriOI GRGCYC 2 cut(s) 826, 2850
FspBI CTAG 7 cut(s) 651, 819, 828, 1217, 1487, 1776, 2040
FspI TGCGCA 1 cut(s) 375
GlaI GCGC 5 cut(s) 107, 165, 280, 375, 1916
GsaI CCCAGC 1 cut(s) 3030
GsuI CTGGAG 1 cut(s) 2232
HaeII RGCGCY 2 cut(s) 109, 167
HaeIII GGCC 3 cut(s) 1290, 1892, 2807
HapII CCGG 4 cut(s) 470, 554, 1464, 1889
HgaI GACGC 3 cut(s) 204, 1992, 2742
HhaI GCGC 5 cut(s) 108, 166, 281, 376, 1917
Hin1I GRCGYC 3 cut(s) 106, 164, 215
Hin6I GCGC 5 cut(s) 106, 164, 279, 374, 1915
HinP1I GCGC 5 cut(s) 106, 164, 279, 374, 1915
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HindIII AAGCTT 1 cut(s) 1707
HinfI GANTC 6 cut(s) 91, 932, 1000, 1097, 1460, 2221
HpaII CCGG 4 cut(s) 470, 554, 1464, 1889
HphI GGTGA 5 cut(s) 455, 545, 1019, 1064, 1913
Hpy166II GTNNAC 5 cut(s) 975, 1324, 1409, 2459, 2979
Hpy188III TCNNGA 8 cut(s) 95, 235, 602, 1464, 1833, 2332, 2414, 2440
Hpy8I GTNNAC 5 cut(s) 975, 1324, 1409, 2459, 2979
Hpy99I CGWCG 1 cut(s) 25
HpyCH4III ACNGT 4 cut(s) 175, 1037, 1790, 2694
HpyCH4IV ACGT 2 cut(s) 1761, 3004
HpyF3I CTNAG 5 cut(s) 264, 807, 2300, 2817, 3072
HpySE526I ACGT 2 cut(s) 1761, 3004
Hsp92I GRCGYC 3 cut(s) 106, 164, 215
HspAI GCGC 5 cut(s) 106, 164, 279, 374, 1915
KasI GGCGCC 2 cut(s) 105, 163
Kpn2I TCCGGA 1 cut(s) 1463
KpnI GGTACC 1 cut(s) 2832
Ksp22I TGATCA 2 cut(s) 2416, 2500
KspI CCGCGG 1 cut(s) 332
LguI GCTCTTC 1 cut(s) 1566
LmnI GCTCC 8 cut(s) 271, 351, 829, 1466, 1759, 1958, 2560, 3058
LweI GCATC 5 cut(s) 670, 1448, 2458, 2811, 3071
MabI ACCWGGT 1 cut(s) 1879
MaeI CTAG 7 cut(s) 651, 819, 828, 1217, 1487, 1776, 2040
MaeII ACGT 2 cut(s) 1761, 3004
MaeIII GTNAC 6 cut(s) 4, 450, 549, 2509, 2812, 2912
MbiI CCGCTC 3 cut(s) 127, 346, 1906
MfeI CAATTG 1 cut(s) 2139
MflI RGATCY 2 cut(s) 940, 1183
MhlI GDGCHC 5 cut(s) 826, 1561, 1756, 2565, 2850
Mly113I GGCGCC 2 cut(s) 106, 164
MlyI GAGTC 2 cut(s) 941, 1454
MmeI TCCRAC 4 cut(s) 54, 391, 1516, 1855
MroI TCCGGA 1 cut(s) 1463
MroXI GAANNNNTTC 1 cut(s) 862
MseI TTAA 9 cut(s) 188, 1227, 1262, 1641, 2352, 2706, 2777, 2940, 2949
MslI CAYNNNNRTG 5 cut(s) 38, 756, 2529, 2568, 2961
MspA1I CMGCKG 6 cut(s) 331, 379, 722, 950, 1514, 1622
MspCI CTTAAG 1 cut(s) 1261
MspI CCGG 4 cut(s) 470, 554, 1464, 1889
MspR9I CCNGG 6 cut(s) 471, 555, 1881, 1894, 2804, 3059
MunI CAATTG 1 cut(s) 2139
Mva1269I GAATGC 4 cut(s) 664, 1117, 2968, 3045
MvaI CCWGG 4 cut(s) 1881, 1894, 2804, 3059
MvnI CGCG 4 cut(s) 96, 194, 279, 331
NarI GGCGCC 2 cut(s) 106, 164
NciI CCSGG 2 cut(s) 471, 555
NcoI CCATGG 1 cut(s) 870
NdeI CATATG 3 cut(s) 757, 1810, 2530
NheI GCTAGC 2 cut(s) 650, 1775
NmeAIII GCCGAG 1 cut(s) 1316
NmuCI GTSAC 4 cut(s) 4, 450, 2509, 2912
NruI TCGCGA 1 cut(s) 96
NsbI TGCGCA 1 cut(s) 375
NspI RCATGY 5 cut(s) 37, 88, 2075, 2291, 2666
PagI TCATGA 1 cut(s) 234
PciI ACATGT 1 cut(s) 33
PciSI GCTCTTC 1 cut(s) 1566
PctI GAATGC 4 cut(s) 664, 1117, 2968, 3045
PdmI GAANNNNTTC 1 cut(s) 862
PfeI GAWTC 4 cut(s) 91, 1000, 1097, 2221
PflFI GACNNNGTC 1 cut(s) 25
PflMI CCANNNNNTGG 4 cut(s) 848, 851, 1811, 1847
PleI GAGTC 2 cut(s) 940, 1454
PluTI GGCGCC 2 cut(s) 109, 167
PmaCI CACGTG 1 cut(s) 3005
PmlI CACGTG 1 cut(s) 3005
PpsI GAGTC 2 cut(s) 940, 1454
Ppu21I YACGTR 1 cut(s) 3005
PpuMI RGGWCCY 1 cut(s) 1268
PscI ACATGT 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 1227
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 4 cut(s) 1879, 1892, 2802, 3057
PspCI CACGTG 1 cut(s) 3005
PspEI GGTNACC 2 cut(s) 4, 549
PspFI CCCAGC 1 cut(s) 3026
PspGI CCWGG 4 cut(s) 1879, 1892, 2802, 3057
PspPI GGNCC 8 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359, 2806
PspPPI RGGWCCY 1 cut(s) 1268
PstI CTGCAG 2 cut(s) 523, 940
PstNI CAGNNNCTG 1 cut(s) 1514
PsuI RGATCY 2 cut(s) 940, 1183
PsyI GACNNNGTC 1 cut(s) 25
PvuII CAGCTG 3 cut(s) 722, 950, 1622
RruI TCGCGA 1 cut(s) 96
RsaI GTAC 7 cut(s) 68, 1082, 1549, 1598, 2460, 2830, 2892
RsaNI GTAC 7 cut(s) 67, 1081, 1548, 1597, 2459, 2829, 2891
RseI CAYNNNNRTG 5 cut(s) 38, 756, 2529, 2568, 2961
SacII CCGCGG 1 cut(s) 332
SapI GCTCTTC 1 cut(s) 1566
SaqAI TTAA 9 cut(s) 188, 1227, 1262, 1641, 2352, 2706, 2777, 2940, 2949
Sau96I GGNCC 8 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359, 2806
ScaI AGTACT 1 cut(s) 1598
SchI GAGTC 2 cut(s) 941, 1454
ScrFI CCNGG 6 cut(s) 471, 555, 1881, 1894, 2804, 3059
SduI GDGCHC 5 cut(s) 826, 1561, 1756, 2565, 2850
SexAI ACCWGGT 1 cut(s) 1879
SfaNI GCATC 5 cut(s) 670, 1448, 2458, 2811, 3071
SfcI CTRYAG 3 cut(s) 519, 936, 2340
SfoI GGCGCC 2 cut(s) 107, 165
Sfr303I CCGCGG 1 cut(s) 332
SgrBI CCGCGG 1 cut(s) 332
SinI GGWCC 7 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359
SmiMI CAYNNNNRTG 5 cut(s) 38, 756, 2529, 2568, 2961
SmlI CTYRAG 3 cut(s) 628, 1261, 1600
SmoI CTYRAG 3 cut(s) 628, 1261, 1600
SspDI GGCGCC 2 cut(s) 105, 163
SspMI CTAG 7 cut(s) 651, 819, 828, 1217, 1487, 1776, 2040
StyD4I CCNGG 6 cut(s) 469, 553, 1879, 1892, 2802, 3057
StyI CCWWGG 2 cut(s) 870, 1393
TaaI ACNGT 4 cut(s) 175, 1037, 1790, 2694
TaiI ACGT 2 cut(s) 1764, 3007
TaqI TCGA 4 cut(s) 20, 47, 835, 2323
TatI WGTACW 3 cut(s) 66, 1596, 2890
TauI GCSGC 8 cut(s) 63, 169, 334, 343, 346, 506, 1514, 2179
TfiI GAWTC 4 cut(s) 91, 1000, 1097, 2221
Tru1I TTAA 9 cut(s) 188, 1227, 1262, 1641, 2352, 2706, 2777, 2940, 2949
Tru9I TTAA 9 cut(s) 188, 1227, 1262, 1641, 2352, 2706, 2777, 2940, 2949
TscAI CASTG 5 cut(s) 1174, 2092, 2287, 2570, 2839
TseFI GTSAC 4 cut(s) 4, 450, 2509, 2912
Tsp45I GTSAC 4 cut(s) 4, 450, 2509, 2912
TspGWI ACGGA 1 cut(s) 2605
TspRI CASTG 5 cut(s) 1174, 2092, 2287, 2570, 2839
Tth111I GACNNNGTC 1 cut(s) 25
Van91I CCANNNNNTGG 4 cut(s) 848, 851, 1811, 1847
Vha464I CTTAAG 1 cut(s) 1261
VpaK11BI GGWCC 7 cut(s) 1268, 1490, 1579, 1850, 1877, 2272, 2359
VspI ATTAAT 1 cut(s) 1227
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 4 cut(s) 837, 1075, 1984, 2345
XceI RCATGY 5 cut(s) 37, 88, 2075, 2291, 2666
XmiI GTMKAC 2 cut(s) 1323, 2978
XmnI GAANNNNTTC 1 cut(s) 862
XspI CTAG 7 cut(s) 651, 819, 828, 1217, 1487, 1776, 2040
ZrmI AGTACT 1 cut(s) 1598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.