FvH4_3g36870

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002776
Physical Location & Seq
Forward (+)
31577751 .. 31585582
7832 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36870.t1

Sequence Viewer

Length: 3234 bp
ATGTTGTCTGAATTGGGGAGAAGACCAATGCTTGGAGGTAATGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTAGTATGTTGCTGAGGGACCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGATTTGAATATCTATAGGAGTGGGTCAGCGCCTCCGACTGTGGAGGGTTCTTTGAATGCTGTGGGAGGGTTGTTTGCTGGGGGTGGTGGTGGAGGAGGCGGTGTGGGCGGTGGTGTTGCTGGCTCTTTCCTATCGGAGTTTCCTGGAGCTAAAAATGGAAATGGGTTCTCGTCGGAGGAGGAGATGAGGTCTGATCCAGCTTATCTGAAATACTATTACTCGAATGTGAACATGAATCCTAGGCTTCCACCTCCATTGTTGTCAAAGGAGGATTGGAGGTGTGCGCAGAGGATGAAAGGAGGGAGTTCAGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGGAGAGCCATGTATTCGATGCCACCCGGTTTTAACTCGAGGAAACAAGAGAGTGATGTGGAGCCGGATAAAGTACGTGGCTCAGCAGAGTGGGGTAATGATGGGCTAATTGGTCTGCCTGGTTTAGGACTTGGTAACAAACAAAAAAGCCTTGCTGAAATCTTTCAGGATGACATGGGGCGTACAACTCCTGTTCCTGGGCTTCCTTCTCGTCCCGCTAGTCGTAATGCATTTGATGAAAATGTGGAGGCACTTGGTTCTGCTGAAGCTGACCTGACTCATTTGCGCCGTGACTTGATGACATCTGATGCTCTACGATCTGGTGCAAATGGACAGGGTTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATATTCCTATGCTGCAGCTTTAGGTGCTTCCTTGTCAAGAAGCACGACCCCTGATCCTCAGGTTATTGCTAGGGCTCCGAGCCCTTGTCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAAAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATTAATGAGTCCGGAGACATAGTAGCTGCCTTATCCACCATGAACTTGTCTTCAAATGGTGTGATAGATGATGAGCCCCATTTGCCATCACAGGTTAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGCCTGCAAGGTGCTGAGAGTCATGCCAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGCGCATATACATATGCCTTCTCCACAGTCAGCAAAAGGTTCGTACTTGGATTTGGGTAAGAGCAATGGGGTTGGCTCAGATCAGAACATCGCATCCTCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTGTTAACTTGTACAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAACTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCATTGGCATCCATGGTGGCTAGTCAACTTGGTACTGGCAATCTGCCACCTTTGTTTGAAAATGTTGCTGCAGCATCAGCTATGATACCTCCTGGAATGGATTCAAGAGTTCTTGGAGGAGGTTTGGCCTCTGGACCAAACTTAGCAGCTGCTGCATCTGATTCACATAACCTTGGTAGATTAGGGAGTCCGATAGCTGGGAACGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCTGAGTATGCTGCAGCACAGCTTGCTGCTCTGAATGATCCCTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATATACTTGAACTTCAAAAGGCATATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGAGTTGGTGCTCCCTTGGGGGGCAAGAGTGGTGGTTCAAATCATCACGGTTACTACGGAAATCATGCCTTTGGCATGTCTTATCCTGGAAGTCCCATGGCAAGTCCTGTTATTCCCAACTCTCCAGTTGGCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATGCTGGATGTAACTTAGATGAAAGCTTTGCATCTTCACTCTTAGAAGAGTTTAAGAGCAATAAAGCCAAGTCCTTCGAACTTTCAGAAATCGGTGGACATGTTGTGGAATTCAGTGCGGATCAATATGGGAGTCGTTTTATTCAACAAAAACTTGAGACAGCCACTACGGAAGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTCGCTCTAATGACCGATGTATTTGGTAATTATGTGATTCAGAAGTTTTTTGAGCACGGGCTTCCGTCTCAGAGAAGGGAGTTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTCCAAATGTATGGTTGCCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAAAAGATCAAAATGGTTGGAGAACTTGATGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTGATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACCCATCCATATGGGTGTCGTGTAATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAGGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATTATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAGTGTTTAGCTTTTGGCGGTCCTGCTGAGCGTGAGTTACTAGTGAATGAGATGCTTGGAACTACTGATGAGAATGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAAACTTGTGATGACCAACAACGTGAGCTGATTCTTTCACGAATTAAAGTTCACTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTTGCTCGTGTAGAAAAACTTGTTGCCGCTGGGGAAAGGAGAGTTGCTGCTGCTGCTCAGTCGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1078

Amino Acids

115.37

Weight (kDa)

5.92

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 312 - 738 3.2e-173 Nucleic acid binding protein NABP
PUF PF00806 743 - 777 9.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 781 - 812 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 815 - 848 8.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 851 - 932 1.5e-07 NOP9-like PUF repeat domain
PUF PF00806 857 - 881 1e-05 Pumilio-family RNA binding repeat
PUF PF00806 887 - 922 7.3e-13 Pumilio-family RNA binding repeat
PUF PF00806 927 - 957 8.3e-10 Pumilio-family RNA binding repeat
PUF PF00806 961 - 994 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1006 - 1035 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2861
Acc16I TGCGCA 2 cut(s) 414, 1233
AccB1I GGYRCC 1 cut(s) 1714
AccB7I CCANNNNNTGG 2 cut(s) 32, 847
AccIII TCCGGA 1 cut(s) 1049
AciI CCGC 6 cut(s) 228, 237, 708, 2246, 2943, 3180
AclI AACGTT 1 cut(s) 2648
AclWI GGATC 7 cut(s) 317, 911, 1393, 1721, 1787, 2256, 2799
AcoI YGGCCR 2 cut(s) 2424, 2548
AcsI RAATTY 4 cut(s) 62, 2086, 2092, 2237
AcuI CTGAAG 4 cut(s) 777, 969, 2640, 2993
AcyI GRCGYC 1 cut(s) 1715
AfaI GTAC 7 cut(s) 567, 676, 1274, 1379, 1534, 2823, 3048
AfiI CCNNNNNNNGG 9 cut(s) 32, 617, 689, 847, 965, 1332, 1697, 1916, 2623
AflIII ACRYGT 1 cut(s) 2227
AhlI ACTAGT 1 cut(s) 2965
AjnI CCWGG 8 cut(s) 271, 610, 688, 1591, 1710, 1981, 2029, 2319
AjuI GAANNNNNNNTTGG 4 cut(s) 1487, 1519, 2078, 2110
AloI GAACNNNNNNTCC 2 cut(s) 1307, 1339
Alw21I GWGCWC 3 cut(s) 1909, 2397, 2721
Alw26I GTCTC 6 cut(s) 1033, 1047, 1712, 2279, 2412, 2647
AlwI GGATC 7 cut(s) 317, 911, 1393, 1721, 1787, 2256, 2799
AlwNI CAGNNNCTG 1 cut(s) 1652
Ama87I CYCGRG 1 cut(s) 529
Aor13HI TCCGGA 1 cut(s) 1049
AoxI GGCC 7 cut(s) 850, 1171, 1626, 2026, 2424, 2548, 2625
ApaI GGGCCC 1 cut(s) 854
ApoI RAATTY 4 cut(s) 62, 2086, 2092, 2237
AseI ATTAAT 1 cut(s) 1041
Asp700I GAANNNNTTC 2 cut(s) 654, 1600
AspA2I CCTAGG 1 cut(s) 368
AspLEI GCGC 6 cut(s) 160, 415, 780, 1234, 1717, 2562
AspS9I GGNCC 8 cut(s) 94, 850, 851, 1634, 2027, 2114, 2515, 2945
AsuC2I CCSGG 2 cut(s) 519, 2480
AsuHPI GGTGA 4 cut(s) 1145, 1869, 2494, 3215
AsuII TTCGAA 1 cut(s) 2205
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 5 cut(s) 94, 1634, 2114, 2515, 2945
AvrII CCTAGG 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 921
BaeGI GKGCMC 1 cut(s) 854
BalI TGGCCA 2 cut(s) 2426, 2550
BanI GGYRCC 1 cut(s) 1714
BanII GRGCYC 4 cut(s) 854, 940, 947, 1116
BauI CACGAG 1 cut(s) 3159
BbsI GAAGAC 2 cut(s) 28, 1080
Bbv12I GWGCWC 3 cut(s) 1909, 2397, 2721
BbvCI CCTCAGC 1 cut(s) 89
BccI CCATC 4 cut(s) 587, 1132, 2540, 2688
BceAI ACGGC 1 cut(s) 765
BcgI CGANNNNNNTGC 2 cut(s) 1251, 1285
BciT130I CCWGG 8 cut(s) 273, 612, 690, 1593, 1712, 1983, 2031, 2321
BclI TGATCA 2 cut(s) 1150, 2656
BcnI CCSGG 2 cut(s) 519, 2480
BcoDI GTCTC 6 cut(s) 1033, 1047, 1712, 2279, 2412, 2647
BcuI ACTAGT 1 cut(s) 2965
BfaI CTAG 7 cut(s) 369, 489, 711, 933, 1005, 1521, 2966
BfmI CTRYAG 5 cut(s) 142, 834, 876, 1569, 1767
BfoI RGCGCY 2 cut(s) 161, 1718
BlnI CCTAGG 1 cut(s) 368
BlpI GCTNAGC 2 cut(s) 574, 2952
Bme18I GGWCC 5 cut(s) 94, 1634, 2114, 2515, 2945
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 8 cut(s) 94, 850, 851, 1634, 2027, 2114, 2515, 2945
BmiI GGNNCC 9 cut(s) 95, 555, 852, 939, 1716, 1867, 2115, 2585, 3219
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 28, 1080
BpmI CTGGAG 4 cut(s) 294, 1694, 2004, 2846
Bpu10I CCTNAGC 1 cut(s) 89
Bpu1102I GCTNAGC 2 cut(s) 574, 2952
Bpu14I TTCGAA 1 cut(s) 2205
BpuEI CTTGAG 4 cut(s) 1768, 2303, 2319, 2735
BpuMI CCSGG 2 cut(s) 519, 2480
BsaAI YACGTR 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 1715
BsaI GGTCTC 1 cut(s) 1712
BsaJI CCNNGG 8 cut(s) 368, 689, 1512, 1672, 1911, 1992, 2029, 2117
BsaWI WCCGGW 1 cut(s) 1049
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 617, 689, 847, 965, 1332, 1697, 1916, 2623
Bse1I ACTGG 3 cut(s) 1540, 2021, 2829
Bse21I CCTNAGG 1 cut(s) 921
Bse3DI GCAATG 3 cut(s) 1300, 2731, 3021
BseAI TCCGGA 1 cut(s) 1049
BseBI CCWGG 8 cut(s) 273, 612, 690, 1593, 1712, 1983, 2031, 2321
BseDI CCNNGG 8 cut(s) 368, 689, 1512, 1672, 1911, 1992, 2029, 2117
BseLI CCNNNNNNNGG 9 cut(s) 32, 617, 689, 847, 965, 1332, 1697, 1916, 2623
BseMI GCAATG 3 cut(s) 1300, 2731, 3021
BseNI ACTGG 3 cut(s) 1540, 2021, 2829
BseRI GAGGAG 6 cut(s) 237, 320, 323, 981, 1632, 3210
BseSI GKGCMC 1 cut(s) 854
BseYI CCCAGC 4 cut(s) 206, 1697, 2873, 3182
BshFI GGCC 7 cut(s) 852, 1173, 1628, 2028, 2426, 2550, 2627
BshNI GGYRCC 1 cut(s) 1714
BsiHKAI GWGCWC 3 cut(s) 1909, 2397, 2721
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 4 cut(s) 519, 557, 1050, 2479
BslFI GGGAC 6 cut(s) 107, 470, 690, 1974, 2127, 2600
BslI CCNNNNNNNGG 9 cut(s) 32, 617, 689, 847, 965, 1332, 1697, 1916, 2623
BsmAI GTCTC 6 cut(s) 1033, 1047, 1712, 2279, 2412, 2647
BsmBI CGTCTC 1 cut(s) 2412
BsmFI GGGAC 6 cut(s) 107, 470, 690, 1974, 2127, 2600
BsmI GAATGC 2 cut(s) 190, 3124
BsnI GGCC 7 cut(s) 852, 1173, 1628, 2028, 2426, 2550, 2627
Bso31I GGTCTC 1 cut(s) 1712
BsoBI CYCGRG 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 2205
Bsp120I GGGCCC 1 cut(s) 850
Bsp1286I GDGCHC 7 cut(s) 854, 940, 947, 1116, 1909, 2397, 2721
Bsp13I TCCGGA 1 cut(s) 1049
Bsp1407I TGTACA 2 cut(s) 1377, 3046
Bsp1720I GCTNAGC 2 cut(s) 574, 2952
Bsp19I CCATGG 3 cut(s) 1512, 1992, 2117
BspACI CCGC 6 cut(s) 228, 237, 708, 2246, 2943, 3180
BspANI GGCC 7 cut(s) 852, 1173, 1628, 2028, 2426, 2550, 2627
BspEI TCCGGA 1 cut(s) 1049
BspLI GGNNCC 9 cut(s) 95, 555, 852, 939, 1716, 1867, 2115, 2585, 3219
BspMAI CTGCAG 4 cut(s) 838, 880, 1573, 1771
BspPI GGATC 7 cut(s) 317, 911, 1393, 1721, 1787, 2256, 2799
BspT104I TTCGAA 1 cut(s) 2205
BspT107I GGYRCC 1 cut(s) 1714
BspTNI GGTCTC 1 cut(s) 1712
BsrDI GCAATG 3 cut(s) 1300, 2731, 3021
BsrGI TGTACA 2 cut(s) 1377, 3046
BsrI ACTGG 3 cut(s) 1540, 2021, 2829
BssECI CCNNGG 8 cut(s) 368, 689, 1512, 1672, 1911, 1992, 2029, 2117
BssNI GRCGYC 1 cut(s) 1715
BssSI CACGAG 1 cut(s) 3159
BssT1I CCWWGG 6 cut(s) 368, 1512, 1672, 1911, 1992, 2117
Bst2BI CACGAG 1 cut(s) 3159
Bst2UI CCWGG 8 cut(s) 273, 612, 690, 1593, 1712, 1983, 2031, 2321
Bst4CI ACNGT 5 cut(s) 169, 1257, 1706, 1893, 1946
Bst6I CTCTTC 5 cut(s) 967, 2169, 2295, 2616, 3012
BstACI GRCGYC 1 cut(s) 1715
BstAPI GCANNNNNTGC 2 cut(s) 1652, 1778
BstAUI TGTACA 2 cut(s) 1377, 3046
BstBAI YACGTR 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 2205
BstC8I GCNNGC 5 cut(s) 250, 1175, 1779, 2780, 2784
BstDSI CCRYGG 3 cut(s) 1512, 1992, 2117
BstENI CCTNNNNNAGG 3 cut(s) 615, 963, 1330
BstH2I RGCGCY 2 cut(s) 161, 1718
BstHHI GCGC 6 cut(s) 160, 415, 780, 1234, 1717, 2562
BstMAI GTCTC 6 cut(s) 1033, 1047, 1712, 2279, 2412, 2647
BstNI CCWGG 8 cut(s) 273, 612, 690, 1593, 1712, 1983, 2031, 2321
BstNSI RCATGY 4 cut(s) 1975, 2231, 2782, 2822
BstPAI GACNNNNGTC 1 cut(s) 99
BstSFI CTRYAG 5 cut(s) 142, 834, 876, 1569, 1767
BstSLI GKGCMC 1 cut(s) 854
BstV2I GAAGAC 2 cut(s) 28, 1080
BstXI CCANNNNNNTGG 2 cut(s) 2471, 2687
Bsu36I CCTNAGG 1 cut(s) 921
BsuRI GGCC 7 cut(s) 852, 1173, 1628, 2028, 2426, 2550, 2627
BtgI CCRYGG 3 cut(s) 1512, 1992, 2117
BtgZI GCGATG 1 cut(s) 1303
BtsI GCAGTG 1 cut(s) 2719
BtsIMutI CAGTG 2 cut(s) 2248, 2719
Cac8I GCNNGC 5 cut(s) 250, 1175, 1779, 2780, 2784
CaiI CAGNNNCTG 1 cut(s) 1652
CfoI GCGC 6 cut(s) 160, 415, 780, 1234, 1717, 2562
Cfr13I GGNCC 8 cut(s) 94, 850, 851, 1634, 2027, 2114, 2515, 2945
CseI GACGC 1 cut(s) 89
Csp6I GTAC 7 cut(s) 566, 675, 1273, 1378, 1533, 2822, 3047
CspCI CAANNNNNGTGG 4 cut(s) 366, 401, 1909, 1944
CviQI GTAC 7 cut(s) 566, 675, 1273, 1378, 1533, 2822, 3047
DinI GGCGCC 1 cut(s) 1716
EaeI YGGCCR 2 cut(s) 2424, 2548
Eam1104I CTCTTC 5 cut(s) 967, 2169, 2295, 2616, 3012
EarI CTCTTC 5 cut(s) 967, 2169, 2295, 2616, 3012
Eco130I CCWWGG 6 cut(s) 368, 1512, 1672, 1911, 1992, 2117
Eco24I GRGCYC 4 cut(s) 854, 940, 947, 1116
Eco31I GGTCTC 1 cut(s) 1712
Eco47I GGWCC 5 cut(s) 94, 1634, 2114, 2515, 2945
Eco57I CTGAAG 4 cut(s) 777, 969, 2640, 2993
Eco81I CCTNAGG 1 cut(s) 921
Eco88I CYCGRG 1 cut(s) 529
EcoNI CCTNNNNNAGG 3 cut(s) 615, 963, 1330
EcoRI GAATTC 1 cut(s) 2237
EcoRII CCWGG 8 cut(s) 271, 610, 688, 1591, 1710, 1981, 2029, 2319
EcoT14I CCWWGG 6 cut(s) 368, 1512, 1672, 1911, 1992, 2117
EcoT22I ATGCAT 2 cut(s) 724, 3124
EcoT38I GRGCYC 4 cut(s) 854, 940, 947, 1116
EgeI GGCGCC 1 cut(s) 1716
EheI GGCGCC 1 cut(s) 1716
ErhI CCWWGG 6 cut(s) 368, 1512, 1672, 1911, 1992, 2117
Esp3I CGTCTC 1 cut(s) 2412
FaqI GGGAC 6 cut(s) 107, 470, 690, 1974, 2127, 2600
FauI CCCGC 1 cut(s) 715
FauNDI CATATG 2 cut(s) 1242, 2686
FbaI TGATCA 2 cut(s) 1150, 2656
FriOI GRGCYC 4 cut(s) 854, 940, 947, 1116
FspBI CTAG 7 cut(s) 369, 489, 711, 933, 1005, 1521, 2966
FspI TGCGCA 2 cut(s) 414, 1233
GlaI GCGC 6 cut(s) 159, 414, 779, 1233, 1716, 2561
GsaI CCCAGC 4 cut(s) 210, 1701, 2877, 3186
GsuI CTGGAG 4 cut(s) 294, 1694, 2004, 2846
HaeII RGCGCY 2 cut(s) 161, 1718
HaeIII GGCC 7 cut(s) 852, 1173, 1628, 2028, 2426, 2550, 2627
HapII CCGG 4 cut(s) 519, 557, 1050, 2479
HgaI GACGC 1 cut(s) 89
HhaI GCGC 6 cut(s) 160, 415, 780, 1234, 1717, 2562
Hin1I GRCGYC 1 cut(s) 1715
Hin6I GCGC 6 cut(s) 158, 413, 778, 1232, 1715, 2560
HinP1I GCGC 6 cut(s) 158, 413, 778, 1232, 1715, 2560
HincII GTYRAC 3 cut(s) 1372, 1526, 2897
HindII GTYRAC 3 cut(s) 1372, 1526, 2897
HindIII AAGCTT 2 cut(s) 2152, 2432
HpaI GTTAAC 1 cut(s) 1372
HpaII CCGG 4 cut(s) 519, 557, 1050, 2479
HphI GGTGA 4 cut(s) 1145, 1869, 2494, 3215
Hpy166II GTNNAC 8 cut(s) 358, 472, 1372, 1526, 2225, 2317, 2897, 3112
Hpy188III TCNNGA 9 cut(s) 122, 659, 900, 1050, 1605, 1632, 2126, 2513, 3099
Hpy8I GTNNAC 8 cut(s) 358, 472, 1372, 1526, 2225, 2317, 2897, 3112
Hpy99I CGWCG 2 cut(s) 105, 304
HpyAV CCTTC 9 cut(s) 64, 708, 867, 1257, 1374, 2082, 2212, 2409, 2485
HpyCH4III ACNGT 5 cut(s) 169, 1257, 1706, 1893, 1946
HpyCH4IV ACGT 4 cut(s) 568, 2648, 3042, 3082
HpySE526I ACGT 4 cut(s) 568, 2648, 3042, 3082
Hsp92I GRCGYC 1 cut(s) 1715
HspAI GCGC 6 cut(s) 158, 413, 778, 1232, 1715, 2560
KasI GGCGCC 1 cut(s) 1714
Kpn2I TCCGGA 1 cut(s) 1049
Ksp22I TGATCA 2 cut(s) 1150, 2656
KspAI GTTAAC 1 cut(s) 1372
LmnI GCTCC 9 cut(s) 275, 553, 943, 1019, 1865, 1912, 2768, 2827, 3223
MaeI CTAG 7 cut(s) 369, 489, 711, 933, 1005, 1521, 2966
MaeII ACGT 4 cut(s) 568, 2648, 3042, 3082
MaeIII GTNAC 9 cut(s) 626, 782, 1875, 1887, 1946, 2138, 2570, 2665, 2961
MhlI GDGCHC 7 cut(s) 854, 940, 947, 1116, 1909, 2397, 2721
MlsI TGGCCA 2 cut(s) 2426, 2550
MluNI TGGCCA 2 cut(s) 2426, 2550
Mly113I GGCGCC 1 cut(s) 1715
MlyI GAGTC 6 cut(s) 763, 1055, 1198, 1696, 2269, 2902
MmeI TCCRAC 4 cut(s) 188, 282, 2515, 3039
Mox20I TGGCCA 2 cut(s) 2426, 2550
Mph1103I ATGCAT 2 cut(s) 724, 3124
MroI TCCGGA 1 cut(s) 1049
MroXI GAANNNNTTC 2 cut(s) 654, 1600
MscI TGGCCA 2 cut(s) 2426, 2550
MslI CAYNNNNRTG 5 cut(s) 2127, 2685, 2689, 2724, 3117
Msp20I TGGCCA 2 cut(s) 2426, 2550
MspA1I CMGCKG 2 cut(s) 1649, 3182
MspI CCGG 4 cut(s) 519, 557, 1050, 2479
Mva1269I GAATGC 2 cut(s) 190, 3124
MvaI CCWGG 8 cut(s) 273, 612, 690, 1593, 1712, 1983, 2031, 2321
NarI GGCGCC 1 cut(s) 1715
NciI CCSGG 2 cut(s) 519, 2480
NcoI CCATGG 3 cut(s) 1512, 1992, 2117
NdeI CATATG 2 cut(s) 1242, 2686
NlaIV GGNNCC 9 cut(s) 95, 555, 852, 939, 1716, 1867, 2115, 2585, 3219
NmuCI GTSAC 5 cut(s) 782, 1875, 1887, 2570, 2665
NsbI TGCGCA 2 cut(s) 414, 1233
NsiI ATGCAT 2 cut(s) 724, 3124
NspI RCATGY 4 cut(s) 1975, 2231, 2782, 2822
NspV TTCGAA 1 cut(s) 2205
PaeI GCATGC 1 cut(s) 2782
PaeR7I CTCGAG 1 cut(s) 529
PciI ACATGT 1 cut(s) 2227
PctI GAATGC 2 cut(s) 190, 3124
PdmI GAANNNNTTC 2 cut(s) 654, 1600
PfeI GAWTC 9 cut(s) 364, 1226, 1399, 1495, 1601, 1661, 2377, 2593, 3091
PflMI CCANNNNNTGG 2 cut(s) 32, 847
PfoI TCCNGGA 3 cut(s) 271, 1591, 1981
PleI GAGTC 6 cut(s) 763, 1054, 1197, 1695, 2268, 2901
PluTI GGCGCC 1 cut(s) 1718
PpsI GAGTC 6 cut(s) 763, 1054, 1197, 1695, 2268, 2901
Ppu21I YACGTR 1 cut(s) 569
PscI ACATGT 1 cut(s) 2227
PshAI GACNNNNGTC 1 cut(s) 99
PshBI ATTAAT 1 cut(s) 1041
PsiI TTATAA 1 cut(s) 2861
Psp1406I AACGTT 1 cut(s) 2648
Psp6I CCWGG 8 cut(s) 271, 610, 688, 1591, 1710, 1981, 2029, 2319
PspFI CCCAGC 4 cut(s) 206, 1697, 2873, 3182
PspGI CCWGG 8 cut(s) 271, 610, 688, 1591, 1710, 1981, 2029, 2319
PspN4I GGNNCC 9 cut(s) 95, 555, 852, 939, 1716, 1867, 2115, 2585, 3219
PspOMI GGGCCC 1 cut(s) 850
PspPI GGNCC 8 cut(s) 94, 850, 851, 1634, 2027, 2114, 2515, 2945
PspXI VCTCGAGB 1 cut(s) 529
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 838, 880, 1573, 1771
PstNI CAGNNNCTG 1 cut(s) 1652
PvuII CAGCTG 1 cut(s) 1649
RsaI GTAC 7 cut(s) 567, 676, 1274, 1379, 1534, 2823, 3048
RsaNI GTAC 7 cut(s) 566, 675, 1273, 1378, 1533, 2822, 3047
RseI CAYNNNNRTG 5 cut(s) 2127, 2685, 2689, 2724, 3117
Sau96I GGNCC 8 cut(s) 94, 850, 851, 1634, 2027, 2114, 2515, 2945
SchI GAGTC 6 cut(s) 763, 1055, 1198, 1696, 2269, 2902
SduI GDGCHC 7 cut(s) 854, 940, 947, 1116, 1909, 2397, 2721
SfcI CTRYAG 5 cut(s) 142, 834, 876, 1569, 1767
SfoI GGCGCC 1 cut(s) 1716
Sfr274I CTCGAG 1 cut(s) 529
SfuI TTCGAA 1 cut(s) 2205
SinI GGWCC 5 cut(s) 94, 1634, 2114, 2515, 2945
SlaI CTCGAG 1 cut(s) 529
SmiMI CAYNNNNRTG 5 cut(s) 2127, 2685, 2689, 2724, 3117
SmlI CTYRAG 5 cut(s) 529, 1747, 2282, 2334, 2714
SmoI CTYRAG 5 cut(s) 529, 1747, 2282, 2334, 2714
SpeI ACTAGT 1 cut(s) 2965
SphI GCATGC 1 cut(s) 2782
SsiI CCGC 6 cut(s) 228, 237, 708, 2246, 2943, 3180
SspDI GGCGCC 1 cut(s) 1714
SspMI CTAG 7 cut(s) 369, 489, 711, 933, 1005, 1521, 2966
StyI CCWWGG 6 cut(s) 368, 1512, 1672, 1911, 1992, 2117
TaaI ACNGT 5 cut(s) 169, 1257, 1706, 1893, 1946
TaiI ACGT 4 cut(s) 571, 2651, 3045, 3085
TaqI TCGA 5 cut(s) 350, 509, 530, 1397, 2205
TaqII GACCGA 1 cut(s) 2369
TatI WGTACW 3 cut(s) 1377, 2821, 3046
TauI GCSGC 1 cut(s) 3182
TfiI GAWTC 9 cut(s) 364, 1226, 1399, 1495, 1601, 1661, 2377, 2593, 3091
TscAI CASTG 2 cut(s) 2248, 2726
TseFI GTSAC 5 cut(s) 782, 1875, 1887, 2570, 2665
Tsp45I GTSAC 5 cut(s) 782, 1875, 1887, 2570, 2665
TspGWI ACGGA 3 cut(s) 1968, 2312, 2394
TspRI CASTG 2 cut(s) 2248, 2726
Van91I CCANNNNNTGG 2 cut(s) 32, 847
VpaK11BI GGWCC 5 cut(s) 94, 1634, 2114, 2515, 2945
VspI ATTAAT 1 cut(s) 1041
XagI CCTNNNNNAGG 3 cut(s) 615, 963, 1330
XapI RAATTY 4 cut(s) 62, 2086, 2092, 2237
XceI RCATGY 4 cut(s) 1975, 2231, 2782, 2822
XhoI CTCGAG 1 cut(s) 529
XmaJI CCTAGG 1 cut(s) 368
XmnI GAANNNNTTC 2 cut(s) 654, 1600
XspI CTAG 7 cut(s) 369, 489, 711, 933, 1005, 1521, 2966
Zsp2I ATGCAT 2 cut(s) 724, 3124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.