MD11G1096100.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002776
Physical Location & Seq
Reverse (-)
7977034 .. 7984450
7417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1096100.v1.1.491

Sequence Viewer

Length: 3159 bp
ATGTTGTCGGAATTAGGAAGGAGGCCAGCGCTCGGAGGAAACGAGGTTTCCTTTGGGGATGAATTTGAGAACGAGATAGGGATGCTGCTAAGGGAACAGCGTAGGCAAGACGCCGATGATCGCGAAAGTGAGCTGAATATATATAGAAGCGGATCAGCACCTCCCACTGTGGAGGGTTCTTTGAATGCGGTTGGTGGGTTGTTTGCTGGTGGCGATGGTGGCAGTGCTGGTGCCGCCTTTTCGGAGTTTCCTGGAGCTAAAAATGGAATGGGGTTTGCGTCCGAAGAGGAGCTTAGGTCTGATCCTTCTTATATTCAATACTATTACTCGAATGTGAACCTGAATCCGCGACTTCCTCCTCCTTTGCTGTCGAAGGAGGATTGGAGGTCTGCACAAAGGATGAAAGGAGGAGGGAGTTCGGTGTTGGGTGGGATAGGAGACAGGAGGAAAGTGAATAGGGTTGATGAGGCTAGCGGTAGATCGTTGTTTTCGATGCCGCCAGGTTTCAACTCAAGGAAACAAGAGAGAGACGCCGAGTCGGACAAAGTGCGTGGCTCAGCAGAGTGGGGTGTTGATGGACTGATTGGTTTGCCAGGTTTGGGACTTGGTAACAAACAAAAGAGCCTTGCCGAAATCTTTCAGGATGACTTGGGTCGTGCAGCTCCTGTTTCTGGGCATCCTTCTCGTCCAGCAAGCCGTAATGCATTTGATGAAAATGCAGAGTCAGTTGCTTCTGCTGAATCTGATCTAGCTCATTTGCGACGCGACTTGATGACTTCTGATACTCTACGATCTAGTGCAAATGGTCTTGGATCATCTGCAGCCCAAAGTATGGGCCCGTCTTCTTCATACTCATATGCTGCAGCTCTGGGTGCTTCCTTATCTAGAAGCACCACTCCTGATCCCCAAGTTGTCGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGGGTTGGTGCTTCAGAAAAGAGAGGATTTAGTAGTCCAAGCTCCTTCAATGCTATCTCGTCTGGTATGAATGAGTCTGGGGATCTGGTGGGCGCGTTTTCCAGCATGAACTTGTCTGCCAATGGTGTAAAAGATAACGAGAGCAATTTGCCATCACAGATTAAGCAAGATGTAGATGATCACCAGAATTATCTCTTCGGTCTTCAAGGTGGTGAGAATCATGCCAGGCAACTTGCCTACTTGAAGAAGTCTGAATCTGGGCATATGCACATGCCCTCTGCCCCACACTCAGCAAAAGGTTCATACGCAGATTTGGGTAAGAGCAATGGTGGTGGGCCAGATTCCTCTGATAGGCATGTTGAACTGCAAAAGTCTGCTGTTTCTTCTGGTAATTTATACTCAAAAGGATCTCCCACTTCCAATCTTAGTGGTGGAGGTGGCTTGCATCATCAGTATCAGCGAGTGGATCATGCAAACTCATCATTTGCTAACTATGGTTTAAGTGGGTACTCTATGAATCCAGCATTGGCGTCCATGGTGGCTAGCCAACTTGGCACTGGTAATCTTCCACCCCTGTTTGAATCAGCTATGGGATCCCCTGGAATGGATTCAAGAGTGCTTGGAGGACGTATGGCTTCCGGACCAAACCTAGCAGCCGCTGCAAATGAATCACATAATCTCGCTGGATTAGGGAGTCCGATTGCAGGGAGTGGTCTTCAGGCTCCTTTTGTTGATCCTATGTATCTTCAGTACTTGAGGACATCGGAGTATGCTGCTGCACAGCTTGCTGCTCTTAATGATCCCTCTGTGGATAGGAACTACTTGGGTAATTCATACATGAATTTAATTGAACTTCAAAAGGCATATCTTGGAGCCTTGCTGTCACCGCAGAAGTCACAGTATGGTGTTCCAATGGGGGGAAAGTCTGGTGGTTCTAATCATCATGGGTATTACGGAAATCCTGCATTTGGTGTAGGCATGTCTTATCCTGGAAGTCCTCCTGTTATTCCCAACTCTCCAGTTGGACCAGGTAGTCCTATGAGGCACAACGAACTCAATATATGTTATCCCTCTGGGATGAGGAATTTAGCTCCCTGGCACCTGGATGGTGGATGTAACATAGATGAAAGTTTTGCTTCTTCACTCTTAGAAGAGTTCAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCAGAAATCGTCGGACATGTTGTGGAGTTCAGTGCGGATCAGTATGGAAGCCGTTTTATTCAACAAAAACTTGAAACTGCCACTATAGAAGAGAAACACATGGTTTATCAAGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTACGTGATTCAAAAGTTTTTTGAGCACGGATTTCCATCTCAGAGAAGGGAATTGGCCAACAAACTGTTTAGTCATGTACTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATCCAGAAGGCAATAGAAGTTGTTGATCTGGACCAAAAGATCAAAATGGTTGAAGAACTTGACGGTCATGTAATGCGCTGCGTACGTGATCAAAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATTATCTCAACATTTTTTGATCAAGTTGTGACCCTTTCAACCCATCCATATGGGTGTCGTGTGATACAGAGAGTTCTTGAGCACTGCGAGGATGAGAATACACAGAGTAAAGTTATGGATGAGATTCTAGGAGCTGTTAGCATGTTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTCCTGGAGCATGGAAAACCGCATGAGCGCTCTGCTATCATAAAAGAATTAGCTGGGAAGATAGTCCAAATGAGTCAGCAGAAGTTCGCCTCCAATGTAGTAGAGAAGTGTTTAACTTTTGGCGGTCCTGCTGAACGTGAATTGCTAGTGAATGAGATGCTTGGCACTACTGATGAAAATGAGCCTCTTCAGGCAATGATGAAGGATCAATTTGCAAATTATGTTGTACAAAAAGTGTTGGAGACTTGTGACGACCAGCAACGTGAACTGATTCTTTCCCGAATTAAAGTTCATTTGAATGCATTAAAGAAATACACTTACGGAAAGCATATTGTTGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGCCGCTCAGTCTGCTCAGCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1053

Amino Acids

113.36

Weight (kDa)

5.89

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 307 - 716 6.1e-163 Nucleic acid binding protein NABP
PUF PF00806 721 - 755 3.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 759 - 790 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 793 - 826 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 823 - 909 6.2e-08 NOP9-like PUF repeat domain
PUF PF00806 835 - 859 9e-06 Pumilio-family RNA binding repeat
PUF PF00806 865 - 900 8.9e-13 Pumilio-family RNA binding repeat
PUF PF00806 905 - 935 8.1e-10 Pumilio-family RNA binding repeat
PUF PF00806 939 - 972 2.6e-07 Pumilio-family RNA binding repeat
PUF PF00806 984 - 1013 1.9e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1989
AccB1I GGYRCC 2 cut(s) 230, 2055
AccB7I CCANNNNNTGG 1 cut(s) 832
AccBSI CCGCTC 1 cut(s) 3134
AccII CGCG 4 cut(s) 123, 349, 765, 1052
AccIII TCCGGA 1 cut(s) 1595
AcoI YGGCCR 1 cut(s) 2358
AcsI RAATTY 3 cut(s) 62, 1798, 2041
AcuI CTGAAG 5 cut(s) 954, 1658, 1688, 2574, 2927
AcyI GRCGYC 3 cut(s) 111, 531, 1487
AfaI GTAC 5 cut(s) 1466, 1709, 2382, 2502, 2982
AfeI AGCGCT 2 cut(s) 30, 2783
AflIII ACRYGT 1 cut(s) 2161
AhdI GACNNNNNGTC 1 cut(s) 535
AjuI GAANNNNNNNTTGG 2 cut(s) 1466, 1498
Alw21I GWGCWC 2 cut(s) 2331, 2655
Alw26I GTCTC 3 cut(s) 432, 522, 2990
AlwNI CAGNNNCTG 2 cut(s) 665, 1616
Aor13HI TCCGGA 1 cut(s) 1595
Aor51HI AGCGCT 2 cut(s) 30, 2783
AoxI GGCC 5 cut(s) 23, 835, 1292, 2358, 2559
ApaI GGGCCC 1 cut(s) 839
ApoI RAATTY 3 cut(s) 62, 1798, 2041
Asp700I GAANNNNTTC 3 cut(s) 636, 1564, 3024
AspLEI GCGC 4 cut(s) 31, 1052, 2496, 2784
AspS9I GGNCC 7 cut(s) 835, 836, 1292, 1598, 1982, 2449, 2879
AsuHPI GGTGA 4 cut(s) 1130, 1181, 1833, 2428
AsuNHI GCTAGC 2 cut(s) 470, 1499
AvaII GGWCC 4 cut(s) 1598, 1982, 2449, 2879
BaeGI GKGCMC 1 cut(s) 839
BalI TGGCCA 1 cut(s) 2360
BamHI GGATCC 1 cut(s) 1550
BanI GGYRCC 2 cut(s) 230, 2055
BanII GRGCYC 2 cut(s) 839, 925
BauI CACGAG 1 cut(s) 3093
BbsI GAAGAC 3 cut(s) 834, 1151, 1664
Bbv12I GWGCWC 2 cut(s) 2331, 2655
BccI CCATC 6 cut(s) 209, 569, 1117, 2057, 2347, 2622
BceAI ACGGC 2 cut(s) 681, 2181
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BclI TGATCA 3 cut(s) 1135, 2506, 2590
BcoDI GTCTC 3 cut(s) 432, 522, 2990
BfmI CTRYAG 3 cut(s) 819, 861, 2229
BfoI RGCGCY 2 cut(s) 32, 2785
BglI GCCNNNNNGGC 1 cut(s) 1509
BlpI GCTNAGC 2 cut(s) 556, 3144
BmcAI AGTACT 1 cut(s) 1709
Bme18I GGWCC 4 cut(s) 1598, 1982, 2449, 2879
BmeRI GACNNNNNGTC 1 cut(s) 535
BmgT120I GGNCC 7 cut(s) 835, 836, 1292, 1598, 1982, 2449, 2879
BmiI GGNNCC 8 cut(s) 232, 837, 924, 1552, 1680, 1831, 2057, 2519
BmsI GCATC 5 cut(s) 72, 483, 685, 1411, 2901
BmtI GCTAGC 2 cut(s) 474, 1503
BoxI GACNNNNGTC 1 cut(s) 651
BpiI GAAGAC 3 cut(s) 834, 1151, 1664
BplI GAGNNNNNCTC 2 cut(s) 3118, 3150
BpmI CTGGAG 3 cut(s) 273, 1959, 2780
Bpu10I CCTNAGC 2 cut(s) 89, 293
Bpu1102I GCTNAGC 2 cut(s) 556, 3144
BpuEI CTTGAG 4 cut(s) 496, 1732, 2253, 2669
BsaAI YACGTR 2 cut(s) 2308, 2504
BsaBI GATNNNNATC 1 cut(s) 2338
BsaHI GRCGYC 3 cut(s) 111, 531, 1487
BsaJI CCNNGG 3 cut(s) 1491, 1555, 2051
BsaWI WCCGGW 1 cut(s) 1595
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 945, 975
Bse1I ACTGG 2 cut(s) 1519, 1976
Bse3DI GCAATG 2 cut(s) 1288, 2955
Bse8I GATNNNNATC 1 cut(s) 2338
BseAI TCCGGA 1 cut(s) 1595
BseDI CCNNGG 3 cut(s) 1491, 1555, 2051
BseJI GATNNNNATC 1 cut(s) 2338
BseMI GCAATG 2 cut(s) 1288, 2955
BseMII CTCAG 5 cut(s) 570, 1260, 2357, 3149, 3158
BseNI ACTGG 2 cut(s) 1519, 1976
BseRI GAGGAG 4 cut(s) 302, 348, 423, 966
BseSI GKGCMC 1 cut(s) 839
BseYI CCCAGC 2 cut(s) 2807, 3116
BsgI GTGCAG 3 cut(s) 375, 678, 1719
Bsh1236I CGCG 4 cut(s) 123, 349, 765, 1052
BshFI GGCC 5 cut(s) 25, 837, 1294, 2360, 2561
BshNI GGYRCC 2 cut(s) 230, 2055
BsiHKAI GWGCWC 2 cut(s) 2331, 2655
BsiSI CCGG 1 cut(s) 1596
BsiWI CGTACG 1 cut(s) 2500
BslFI GGGAC 2 cut(s) 615, 2534
BsmAI GTCTC 3 cut(s) 432, 522, 2990
BsmBI CGTCTC 1 cut(s) 522
BsmFI GGGAC 2 cut(s) 615, 2534
BsmI GAATGC 2 cut(s) 190, 3058
BsnI GGCC 5 cut(s) 25, 837, 1294, 2360, 2561
Bsp120I GGGCCC 1 cut(s) 835
Bsp1286I GDGCHC 4 cut(s) 839, 925, 2331, 2655
Bsp13I TCCGGA 1 cut(s) 1595
Bsp1407I TGTACA 1 cut(s) 2980
Bsp1720I GCTNAGC 2 cut(s) 556, 3144
Bsp19I CCATGG 1 cut(s) 1491
Bsp68I TCGCGA 1 cut(s) 123
BspANI GGCC 5 cut(s) 25, 837, 1294, 2360, 2561
BspCNI CTCAG 5 cut(s) 569, 1259, 2356, 3148, 3157
BspEI TCCGGA 1 cut(s) 1595
BspFNI CGCG 4 cut(s) 123, 349, 765, 1052
BspLI GGNNCC 8 cut(s) 232, 837, 924, 1552, 1680, 1831, 2057, 2519
BspMAI CTGCAG 2 cut(s) 823, 865
BspOI GCTAGC 2 cut(s) 474, 1503
BspT107I GGYRCC 2 cut(s) 230, 2055
BsrBI CCGCTC 1 cut(s) 3134
BsrDI GCAATG 2 cut(s) 1288, 2955
BsrGI TGTACA 1 cut(s) 2980
BsrI ACTGG 2 cut(s) 1519, 1976
BssECI CCNNGG 3 cut(s) 1491, 1555, 2051
BssNI GRCGYC 3 cut(s) 111, 531, 1487
BssSI CACGAG 1 cut(s) 3093
BssT1I CCWWGG 1 cut(s) 1491
Bst2BI CACGAG 1 cut(s) 3093
Bst4CI ACNGT 4 cut(s) 169, 1857, 2370, 2483
Bst6I CTCTTC 6 cut(s) 279, 1157, 2103, 2229, 2550, 2946
BstACI GRCGYC 3 cut(s) 111, 531, 1487
BstAPI GCANNNNNTGC 2 cut(s) 1616, 1742
BstAUI TGTACA 1 cut(s) 2980
BstBAI YACGTR 2 cut(s) 2308, 2504
BstC8I GCNNGC 6 cut(s) 27, 472, 694, 1400, 1501, 1743
BstDSI CCRYGG 1 cut(s) 1491
BstENI CCTNNNNNAGG 2 cut(s) 948, 1306
BstFNI CGCG 4 cut(s) 123, 349, 765, 1052
BstH2I RGCGCY 2 cut(s) 32, 2785
BstHHI GCGC 4 cut(s) 31, 1052, 2496, 2784
BstMAI GTCTC 3 cut(s) 432, 522, 2990
BstMWI GCNNNNNNNGC 8 cut(s) 219, 233, 872, 1509, 1616, 1742, 1843, 3140
BstNSI RCATGY 6 cut(s) 1231, 1316, 1939, 2165, 2716, 2756
BstPAI GACNNNNGTC 1 cut(s) 651
BstSFI CTRYAG 3 cut(s) 819, 861, 2229
BstSLI GKGCMC 1 cut(s) 839
BstUI CGCG 4 cut(s) 123, 349, 765, 1052
BstV2I GAAGAC 3 cut(s) 834, 1151, 1664
BstX2I RGATCY 3 cut(s) 1039, 1364, 1550
BstXI CCANNNNNNTGG 1 cut(s) 2621
BstYI RGATCY 3 cut(s) 1039, 1364, 1550
BsuRI GGCC 5 cut(s) 25, 837, 1294, 2360, 2561
BtgI CCRYGG 1 cut(s) 1491
BtgZI GCGATG 1 cut(s) 228
BtsI GCAGTG 2 cut(s) 229, 2653
BtsIMutI CAGTG 5 cut(s) 165, 229, 1512, 2182, 2653
BtuMI TCGCGA 1 cut(s) 123
Cac8I GCNNGC 6 cut(s) 27, 472, 694, 1400, 1501, 1743
CaiI CAGNNNCTG 2 cut(s) 665, 1616
CfoI GCGC 4 cut(s) 31, 1052, 2496, 2784
Cfr13I GGNCC 7 cut(s) 835, 836, 1292, 1598, 1982, 2449, 2879
CseI GACGC 5 cut(s) 119, 267, 539, 771, 1476
CsiI ACCWGGT 1 cut(s) 1984
Csp6I GTAC 5 cut(s) 1465, 1708, 2381, 2501, 2981
CspCI CAANNNNNGTGG 6 cut(s) 532, 567, 1270, 1305, 1366, 1401
CviQI GTAC 5 cut(s) 1465, 1708, 2381, 2501, 2981
DrdI GACNNNNNNGTC 1 cut(s) 1989
DriI GACNNNNNGTC 1 cut(s) 535
DseDI GACNNNNNNGTC 1 cut(s) 1989
EaeI YGGCCR 1 cut(s) 2358
Eam1104I CTCTTC 6 cut(s) 279, 1157, 2103, 2229, 2550, 2946
Eam1105I GACNNNNNGTC 1 cut(s) 535
EarI CTCTTC 6 cut(s) 279, 1157, 2103, 2229, 2550, 2946
Eco130I CCWWGG 1 cut(s) 1491
Eco24I GRGCYC 2 cut(s) 839, 925
Eco47I GGWCC 4 cut(s) 1598, 1982, 2449, 2879
Eco47III AGCGCT 2 cut(s) 30, 2783
Eco57I CTGAAG 5 cut(s) 954, 1658, 1688, 2574, 2927
EcoNI CCTNNNNNAGG 2 cut(s) 948, 1306
EcoT14I CCWWGG 1 cut(s) 1491
EcoT22I ATGCAT 2 cut(s) 706, 3058
EcoT38I GRGCYC 2 cut(s) 839, 925
ErhI CCWWGG 1 cut(s) 1491
Esp3I CGTCTC 1 cut(s) 522
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 2077, 2109
FaqI GGGAC 2 cut(s) 615, 2534
FauNDI CATATG 3 cut(s) 856, 1221, 2620
FbaI TGATCA 3 cut(s) 1135, 2506, 2590
FriOI GRGCYC 2 cut(s) 839, 925
GlaI GCGC 4 cut(s) 30, 1051, 2495, 2783
GsaI CCCAGC 2 cut(s) 2811, 3120
GsuI CTGGAG 3 cut(s) 273, 1959, 2780
HaeII RGCGCY 2 cut(s) 32, 2785
HaeIII GGCC 5 cut(s) 25, 837, 1294, 2360, 2561
HapII CCGG 1 cut(s) 1596
HgaI GACGC 5 cut(s) 119, 267, 539, 771, 1476
HhaI GCGC 4 cut(s) 31, 1052, 2496, 2784
Hin1I GRCGYC 3 cut(s) 111, 531, 1487
Hin6I GCGC 4 cut(s) 29, 1050, 2494, 2782
HinP1I GCGC 4 cut(s) 29, 1050, 2494, 2782
HpaII CCGG 1 cut(s) 1596
HphI GGTGA 4 cut(s) 1130, 1181, 1833, 2428
Hpy166II GTNNAC 2 cut(s) 337, 3020
Hpy8I GTNNAC 2 cut(s) 337, 3020
Hpy99I CGWCG 2 cut(s) 765, 2159
HpyCH4III ACNGT 4 cut(s) 169, 1857, 2370, 2483
HpyCH4IV ACGT 5 cut(s) 1585, 2307, 2503, 2890, 3016
HpyF10VI GCNNNNNNNGC 8 cut(s) 219, 233, 872, 1509, 1616, 1742, 1843, 3140
HpySE526I ACGT 5 cut(s) 1585, 2307, 2503, 2890, 3016
Hsp92I GRCGYC 3 cut(s) 111, 531, 1487
HspAI GCGC 4 cut(s) 29, 1050, 2494, 2782
Kpn2I TCCGGA 1 cut(s) 1595
Ksp22I TGATCA 3 cut(s) 1135, 2506, 2590
LweI GCATC 5 cut(s) 72, 483, 685, 1411, 2901
MabI ACCWGGT 1 cut(s) 1984
MaeII ACGT 5 cut(s) 1585, 2307, 2503, 2890, 3016
MaeIII GTNAC 6 cut(s) 608, 1839, 1851, 2072, 2599, 3002
MbiI CCGCTC 1 cut(s) 3134
MflI RGATCY 3 cut(s) 1039, 1364, 1550
MhlI GDGCHC 4 cut(s) 839, 925, 2331, 2655
MlsI TGGCCA 1 cut(s) 2360
MluNI TGGCCA 1 cut(s) 2360
MlyI GAGTC 5 cut(s) 545, 731, 1040, 1660, 2836
MmeI TCCRAC 4 cut(s) 519, 1960, 2137, 2973
Mox20I TGGCCA 1 cut(s) 2360
Mph1103I ATGCAT 2 cut(s) 706, 3058
MroI TCCGGA 1 cut(s) 1595
MroXI GAANNNNTTC 3 cut(s) 636, 1564, 3024
MscI TGGCCA 1 cut(s) 2360
MseI TTAA 7 cut(s) 1119, 1457, 1752, 1802, 2867, 3039, 3059
MslI CAYNNNNRTG 4 cut(s) 2061, 2619, 2623, 3051
Msp20I TGGCCA 1 cut(s) 2360
MspA1I CMGCKG 1 cut(s) 1616
MspI CCGG 1 cut(s) 1596
Mva1269I GAATGC 2 cut(s) 190, 3058
MvnI CGCG 4 cut(s) 123, 349, 765, 1052
MwoI GCNNNNNNNGC 8 cut(s) 219, 233, 872, 1509, 1616, 1742, 1843, 3140
NcoI CCATGG 1 cut(s) 1491
NdeI CATATG 3 cut(s) 856, 1221, 2620
NheI GCTAGC 2 cut(s) 470, 1499
NlaIV GGNNCC 8 cut(s) 232, 837, 924, 1552, 1680, 1831, 2057, 2519
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 4 cut(s) 1839, 1851, 2599, 3002
NruI TCGCGA 1 cut(s) 123
NsiI ATGCAT 2 cut(s) 706, 3058
NspI RCATGY 6 cut(s) 1231, 1316, 1939, 2165, 2716, 2756
PciI ACATGT 1 cut(s) 2161
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 488
PctI GAATGC 2 cut(s) 190, 3058
PdmI GAANNNNTTC 3 cut(s) 636, 1564, 3024
Pfl23II CGTACG 1 cut(s) 2500
PflMI CCANNNNNTGG 1 cut(s) 832
PfoI TCCNGGA 3 cut(s) 250, 1945, 2757
PleI GAGTC 5 cut(s) 544, 730, 1039, 1659, 2835
PpsI GAGTC 5 cut(s) 544, 730, 1039, 1659, 2835
Ppu21I YACGTR 2 cut(s) 2308, 2504
PscI ACATGT 1 cut(s) 2161
PshAI GACNNNNGTC 1 cut(s) 651
PspFI CCCAGC 2 cut(s) 2807, 3116
PspLI CGTACG 1 cut(s) 2500
PspN4I GGNNCC 8 cut(s) 232, 837, 924, 1552, 1680, 1831, 2057, 2519
PspOMI GGGCCC 1 cut(s) 835
PspPI GGNCC 7 cut(s) 835, 836, 1292, 1598, 1982, 2449, 2879
PstI CTGCAG 2 cut(s) 823, 865
PstNI CAGNNNCTG 2 cut(s) 665, 1616
PsuI RGATCY 3 cut(s) 1039, 1364, 1550
RruI TCGCGA 1 cut(s) 123
RsaI GTAC 5 cut(s) 1466, 1709, 2382, 2502, 2982
RsaNI GTAC 5 cut(s) 1465, 1708, 2381, 2501, 2981
RseI CAYNNNNRTG 4 cut(s) 2061, 2619, 2623, 3051
SaqAI TTAA 7 cut(s) 1119, 1457, 1752, 1802, 2867, 3039, 3059
Sau96I GGNCC 7 cut(s) 835, 836, 1292, 1598, 1982, 2449, 2879
ScaI AGTACT 1 cut(s) 1709
SchI GAGTC 5 cut(s) 545, 731, 1040, 1660, 2836
SduI GDGCHC 4 cut(s) 839, 925, 2331, 2655
SexAI ACCWGGT 1 cut(s) 1984
SfaNI GCATC 5 cut(s) 72, 483, 685, 1411, 2901
SfcI CTRYAG 3 cut(s) 819, 861, 2229
SinI GGWCC 4 cut(s) 1598, 1982, 2449, 2879
SmiMI CAYNNNNRTG 4 cut(s) 2061, 2619, 2623, 3051
SmlI CTYRAG 4 cut(s) 511, 1711, 2268, 2648
SmoI CTYRAG 4 cut(s) 511, 1711, 2268, 2648
StyI CCWWGG 1 cut(s) 1491
TaaI ACNGT 4 cut(s) 169, 1857, 2370, 2483
TaiI ACGT 5 cut(s) 1588, 2310, 2506, 2893, 3019
TaqI TCGA 3 cut(s) 329, 371, 491
TaqII GACCGA 1 cut(s) 1145
TatI WGTACW 3 cut(s) 1707, 2380, 2980
TauI GCSGC 4 cut(s) 236, 499, 1616, 3134
Tru1I TTAA 7 cut(s) 1119, 1457, 1752, 1802, 2867, 3039, 3059
Tru9I TTAA 7 cut(s) 1119, 1457, 1752, 1802, 2867, 3039, 3059
TscAI CASTG 5 cut(s) 172, 229, 1519, 2182, 2660
TseFI GTSAC 4 cut(s) 1839, 1851, 2599, 3002
Tsp45I GTSAC 4 cut(s) 1839, 1851, 2599, 3002
TspGWI ACGGA 3 cut(s) 1926, 2346, 3090
TspRI CASTG 5 cut(s) 172, 229, 1519, 2182, 2660
Van91I CCANNNNNTGG 1 cut(s) 832
VpaK11BI GGWCC 4 cut(s) 1598, 1982, 2449, 2879
XagI CCTNNNNNAGG 2 cut(s) 948, 1306
XapI RAATTY 3 cut(s) 62, 1798, 2041
XbaI TCTAGA 1 cut(s) 884
XceI RCATGY 6 cut(s) 1231, 1316, 1939, 2165, 2716, 2756
XcmI CCANNNNNNNNNTGG 1 cut(s) 1511
XmnI GAANNNNTTC 3 cut(s) 636, 1564, 3024
ZrmI AGTACT 1 cut(s) 1709
Zsp2I ATGCAT 2 cut(s) 706, 3058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.