Rh1CG323300

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002776
Physical Location & Seq
Forward (+)
59867006 .. 59874694
7689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG323300.1

Sequence Viewer

Length: 2985 bp
ATGGTTACTGATACGTATTCGAAGATGATGTCCGAGATGTCAATGCGATCCATGCTTAAGAACAACGGCGATTACAGTGAAGATTTGAGCCTTCTGATTCGTCAGCAGAGGCAAGAAGTGAGTGAGCGAGAGAAGGAGCTCAATCCATACAGAAGTGGGTCGGCCCCACCAACCGTAGAAGGGTCGCTGAACGCCGTCGGCGGTCTTTTTGACGCCGTTAACGGCTTTGCGAGCGAGGAGGAGCTAAGGGCTGATCCGGCTTATCATAGCTACTACTACGCCAATGTCAATCTCAATCCGCGGCTACCGCCGCCGCTGAGGTCCAAGGAGGACTGGCGGTTTGCGCAGCGGGGCGGCGCCGGAGGAGGTTCTGGGGTCGGAGGCATTGGGGATAGGAGGAAAGGCGGGAGGGGTGGTGGTGGTGAAGGGTCGCTTTTTTCGGTGCAGCCGGAGAATGGGGCGGCGGCGAGGAATGGAGAGTGGGGCGGAGATGGGCTGATTGGGTTGCCCGGGTTGGGCCTTGGGAGTAGGCAGAAGAGTATTGCCGAGATTCTTCAGGATGATATACAGAATACAACTGGCTCAAGGCACCCTTCTCGTCCAGCTAGCCGGAATGCATTTGATGATGGTGTTGAAACTTCAGATACCCAGTATGCTCAGATGCATCGTGATTTGGCATCTTTAGATGCTTTGCGCTCTGGTGGAAATAAGCAGGGTATGTCTACTGCCCAGAACTTTGGTTCCTCAGGCTCTCATACTTATGCTTCTGCTCTAGGTGGCTCACTGTCAAGAAGCACCACACCAGATCCTCAGCTTGTATCTAGGGCTCCTAGTCCCCGGATTCCACCAGTTGGAGGAGGAAGAGCTCCTTCCACTGATAAAAATAGTGTGGGTGGTCAAAATTCATATAATGGTATCGCATCCAATGTGAAAGAGTCTGCAGATCTGGTAGCTGCCTTATCTGGTATGAACCTGTCAAAAAACAGTAGGATGCATGAAGAGAATCATGCACATTCACAGATTCAAGGTGATAACCACTTTGATATGCAAGGGGATCGGAACCATATTAAACAAAATTCATATATGAACAGGCCTGATTCAGGGAATTATCATTTGCATTCTGTTTCCCAGTCTACCAAAAACTCTTATCAGAATATGGCCAAAGGCAGTGGATTTGGGAGGGACCAGAACAACCCTTCACTGATGTCTGATGGGCTAGCTGAAATTAACAATCCTGCTGTATCTTCTGCTAACTCATACTTGAGAGGACCCTCTGCCCCAGCTCTTAATGGCCGAGGAAGCTCAGTCTCTCACTATCAGAATGTAGACGGTATGAATTCCTCATTTGCAAACTATGGGTTAGCTGGGTATTCTGTTAGTCCATCGTCACCCTCCATGATGGGGAGCCCACTGGGTAATGGTAATTTACCACCCTTGTTTGAAAATGCTGCTGCTGTTTCTGCAATGGGTGGCTTGGACTCTGGAGCCTTTGGAGGAGGTATGTCTCTGGGACCAAATTTGTTAGCAGCAGCTGCTGAATTGCAGAATATGGGGAGGGGCGGAAATCACACTGCCGGAGGTGCTCTTCAGATGCCCCTGATGGACCCCTTGTATCTGCAGTATTTAAGATCAAATGAGTATGCTGCTGCACAGCTTGCATCCCATCATGACCCCACAGCAGACAGGGAAGGCATGTATGTGGACTTTCTGGGGTTGCAAAAAGCTTATCTGGGACAATTACTTTCGCCTCAAAAATCACAGTTTGGTGCTCCCTACATGGGTAAGTCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATTTGGGCTTGGTATGTCTTATTCTGGAAGTCCCCTTCTTCCAAACTCTCCCATTGGACCAGGTAGTCCTGTCAGACACAGTGACAGGAACTTGCGTTTTTCTTCGGGGATGAGGAACATGAGTGGAGGTCTCATGGGAGCTTGGCACTCTGAAACTGGTGGAAATTTCGATGAAAGTTTTGCATCCTCTTTGCTAGATGAGTTCAAGAGCAATAAGACTAAATGTTTTGAACTTTCAGAAATTTCAGGCCATGTTGTTGAGTTCAGTGCGGACCAGTATGGGAGTAGGTTCATTCAACAGAAACTTGAAACTGCCACAACCGAAGAAAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCCCTTTCTTTGATGACTGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTCGAGCACGGTTCTGCAGCACAAATTAGAGAACTGGCTGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGATCTGGACCAGCAGACTCAAATGGTCACAGAACTGGATGGTCACATTATGCGTTGTGTTCGTGATCAAAATGGGAACCATGTCATCCAGAAGTGTATTGAATGTATACCTGAAGATGCTATCCAGTTTGTTGTTTCAACCTTTTATGATCAAGTTGTGACACTGTCAACTCATCCATATGGTTGTCGTGTCATACAGAGAATTTTGGAGCACTGCCATGATCCCAAAACGCAGCAGATAATGATGGATGAGATTTTGCATGCTGTTTGCACTTTGGCACAAGATCAGTATGGAAATTATGTTGTGCAGCATGTGCTGGAGCATGGAAAGCCAGACGAGCGCTCAGATATAATTAGGAAACTAACTGGGCAGATTGTTCAAATGAGCCAGCAGAAATTTGCCTCCAATGTTATAGAAAAGTGTTTAACTTTTGGAACTCTTGCTGAGCGCCAGGCCCTTGTAACTGAGATGCTCGGTACCACTGATGAAAACGAACCCCTTCAGGTTTGTCTTTTATTTGGCTCTATAAGTTTAAAGCACGAACTTTTTCTGAACATATTCACTTATTGTAATTATATTTTTAATTCCTGTCTTTTCTCATTGATCAATCTTCAATATTTGACTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

994

Amino Acids

107.55

Weight (kDa)

5.76

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 275 - 687 4e-149 Nucleic acid binding protein NABP
PUF PF00806 692 - 726 7.7e-12 Pumilio-family RNA binding repeat
PUF PF00806 729 - 761 4.5e-08 Pumilio-family RNA binding repeat
PUF PF00806 764 - 797 1.9e-09 Pumilio-family RNA binding repeat
PUF PF00806 836 - 871 2.3e-13 Pumilio-family RNA binding repeat
PUF PF00806 874 - 906 1.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 909 - 942 2e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1887
Acc16I TGCGCA 1 cut(s) 345
Acc65I GGTACC 1 cut(s) 2831
AccB1I GGYRCC 3 cut(s) 356, 588, 2831
AccB7I CCANNNNNTGG 2 cut(s) 851, 1826
AccI GTMKAC 4 cut(s) 722, 1133, 1326, 2461
AccII CGCG 1 cut(s) 301
AclWI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
AcoI YGGCCR 2 cut(s) 1158, 1291
AcsI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
AcuI CTGAAG 5 cut(s) 539, 624, 1571, 2487, 2840
AcyI GRCGYC 2 cut(s) 213, 357
AfaI GTAC 1 cut(s) 2833
AfeI AGCGCT 1 cut(s) 2696
AfiI CCNNNNNNNGG 9 cut(s) 180, 455, 515, 851, 854, 1100, 1401, 1778, 1826
AflII CTTAAG 1 cut(s) 56
AjnI CCWGG 2 cut(s) 1882, 2805
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
Alw21I GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
Alw26I GTCTC 3 cut(s) 1312, 1509, 1958
AlwI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
AlwNI CAGNNNCTG 2 cut(s) 1532, 1535
Ama87I CYCGRG 1 cut(s) 509
Aor51HI AGCGCT 1 cut(s) 2696
AoxI GGCC 7 cut(s) 162, 517, 1091, 1158, 1291, 2071, 2808
ApoI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
ArsI GACNNNNNNTTYG 4 cut(s) 14, 46, 323, 355
Asp700I GAANNNNTTC 4 cut(s) 2234, 2853, 2901, 2912
Asp718I GGTACC 1 cut(s) 2831
AspLEI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
AsuC2I CCSGG 3 cut(s) 510, 511, 838
AsuHPI GGTGA 3 cut(s) 434, 1040, 1380
AsuII TTCGAA 1 cut(s) 20
AsuNHI GCTAGC 2 cut(s) 605, 1216
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 8 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362
AxyI CCTNAGG 1 cut(s) 745
BalI TGGCCA 1 cut(s) 1160
BanI GGYRCC 3 cut(s) 356, 588, 2831
BanII GRGCYC 4 cut(s) 141, 829, 868, 1409
Bbv12I GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
BbvCI CCTCAGC 2 cut(s) 317, 810
BccI CCATC 9 cut(s) 485, 620, 1205, 1390, 1393, 1594, 1671, 2387, 2593
BceAI ACGGC 4 cut(s) 82, 179, 200, 238
BcgI CGANNNNNNTGC 4 cut(s) 578, 612, 1037, 1071
BciT130I CCWGG 2 cut(s) 1884, 2807
BclI TGATCA 3 cut(s) 2419, 2503, 2958
BcnI CCSGG 3 cut(s) 510, 511, 838
BcoDI GTCTC 3 cut(s) 1312, 1509, 1958
BfaI CTAG 6 cut(s) 606, 773, 822, 831, 1217, 2018
BfmI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
BfoI RGCGCY 3 cut(s) 360, 2698, 2806
BfrI CTTAAG 1 cut(s) 56
BglII AGATCT 1 cut(s) 943
BlpI GCTNAGC 1 cut(s) 2799
Bme1390I CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
Bme18I GGWCC 8 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362
BmeT110I CYCGRG 1 cut(s) 509
BmrFI CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
BmrI ACTGGG 5 cut(s) 643, 1123, 1421, 2295, 2730
BmtI GCTAGC 2 cut(s) 609, 1220
BmuI ACTGGG 5 cut(s) 643, 1123, 1421, 2295, 2730
BpmI CTGGAG 2 cut(s) 1503, 2693
Bpu10I CCTNAGC 4 cut(s) 245, 317, 810, 2303
Bpu1102I GCTNAGC 1 cut(s) 2799
Bpu14I TTCGAA 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 568, 1282
BpuMI CCSGG 3 cut(s) 510, 511, 838
BsaAI YACGTR 1 cut(s) 15
BsaHI GRCGYC 2 cut(s) 213, 357
BsaI GGTCTC 1 cut(s) 1958
BsaJI CCNNGG 6 cut(s) 299, 324, 509, 520, 836, 1294
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 9 cut(s) 180, 455, 515, 851, 854, 1100, 1401, 1778, 1826
Bse21I CCTNAGG 1 cut(s) 745
Bse3DI GCAATG 1 cut(s) 1470
BseBI CCWGG 2 cut(s) 1884, 2807
BseDI CCNNGG 6 cut(s) 299, 324, 509, 520, 836, 1294
BseLI CCNNNNNNNGG 9 cut(s) 180, 455, 515, 851, 854, 1100, 1401, 1778, 1826
BseMI GCAATG 1 cut(s) 1470
BseMII CTCAG 8 cut(s) 308, 671, 759, 824, 1317, 2712, 2790, 2811
BseRI GAGGAG 5 cut(s) 251, 254, 378, 870, 1509
BseYI CCCAGC 2 cut(s) 1279, 1364
BsgI GTGCAG 3 cut(s) 464, 1632, 2681
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 7 cut(s) 164, 519, 1093, 1160, 1293, 2073, 2810
BshNI GGYRCC 3 cut(s) 356, 588, 2831
BsiHKAI GWGCWC 6 cut(s) 141, 868, 1585, 1771, 2244, 2568
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
BslFI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
BslI CCNNNNNNNGG 9 cut(s) 180, 455, 515, 851, 854, 1100, 1401, 1778, 1826
BsmAI GTCTC 3 cut(s) 1312, 1509, 1958
BsmFI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
BsmI GAATGC 2 cut(s) 619, 1117
BsnI GGCC 7 cut(s) 164, 519, 1093, 1160, 1293, 2073, 2810
Bso31I GGTCTC 1 cut(s) 1958
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 8 cut(s) 141, 829, 868, 1409, 1585, 1771, 2244, 2568
Bsp1720I GCTNAGC 1 cut(s) 2799
BspANI GGCC 7 cut(s) 164, 519, 1093, 1160, 1293, 2073, 2810
BspCNI CTCAG 8 cut(s) 309, 670, 758, 823, 1316, 2711, 2791, 2812
BspFNI CGCG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 1666
BspMAI CTGCAG 3 cut(s) 943, 1620, 2254
BspOI GCTAGC 2 cut(s) 609, 1220
BspPI GGATC 6 cut(s) 42, 248, 800, 1062, 2326, 2570
BspQI GCTCTTC 2 cut(s) 856, 1590
BspT104I TTCGAA 1 cut(s) 20
BspT107I GGYRCC 3 cut(s) 356, 588, 2831
BspTI CTTAAG 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 1958
BsrDI GCAATG 1 cut(s) 1470
BssECI CCNNGG 6 cut(s) 299, 324, 509, 520, 836, 1294
BssNAI GTATAC 1 cut(s) 2462
BssNI GRCGYC 2 cut(s) 213, 357
BssT1I CCWWGG 2 cut(s) 324, 520
Bst1107I GTATAC 1 cut(s) 2462
Bst2UI CCWGG 2 cut(s) 1884, 2807
Bst6I CTCTTC 4 cut(s) 530, 856, 993, 1590
BstACI GRCGYC 2 cut(s) 213, 357
BstAFI CTTAAG 1 cut(s) 56
BstAPI GCANNNNNTGC 3 cut(s) 1532, 1655, 2668
BstBAI YACGTR 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 6 cut(s) 232, 607, 1218, 1656, 2616, 2744
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 1098
BstFNI CGCG 1 cut(s) 301
BstH2I RGCGCY 3 cut(s) 360, 2698, 2806
BstHHI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
BstMAI GTCTC 3 cut(s) 1312, 1509, 1958
BstNI CCWGG 2 cut(s) 1884, 2807
BstNSI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
BstSCI CCNGG 5 cut(s) 508, 509, 836, 1882, 2805
BstSFI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
BstSNI TACGTA 1 cut(s) 15
BstUI CGCG 1 cut(s) 301
BstX2I RGATCY 2 cut(s) 805, 943
BstXI CCANNNNNNTGG 2 cut(s) 737, 2285
BstYI RGATCY 2 cut(s) 805, 943
BstZ17I GTATAC 1 cut(s) 2462
Bsu36I CCTNAGG 1 cut(s) 745
BsuRI GGCC 7 cut(s) 164, 519, 1093, 1160, 1293, 2073, 2810
BtgI CCRYGG 1 cut(s) 299
BtsI GCAGTG 3 cut(s) 1174, 1569, 2566
Cac8I GCNNGC 6 cut(s) 232, 607, 1218, 1656, 2616, 2744
CaiI CAGNNNCTG 2 cut(s) 1532, 1535
CciI TCATGA 1 cut(s) 1666
CfoI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
Cfr42I CCGCGG 1 cut(s) 302
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 1882
Csp6I GTAC 1 cut(s) 2832
CspCI CAANNNNNGTGG 2 cut(s) 1150, 1185
CviQI GTAC 1 cut(s) 2832
DinI GGCGCC 1 cut(s) 358
DraI TTTAAA 1 cut(s) 2889
DrdI GACNNNNNNGTC 1 cut(s) 1887
DseDI GACNNNNNNGTC 1 cut(s) 1887
EaeI YGGCCR 2 cut(s) 1158, 1291
Eam1104I CTCTTC 4 cut(s) 530, 856, 993, 1590
EarI CTCTTC 4 cut(s) 530, 856, 993, 1590
EciI GGCGGA 2 cut(s) 501, 1575
Ecl136II GAGCTC 2 cut(s) 139, 866
Eco105I TACGTA 1 cut(s) 15
Eco130I CCWWGG 2 cut(s) 324, 520
Eco147I AGGCCT 1 cut(s) 1093
Eco24I GRGCYC 4 cut(s) 141, 829, 868, 1409
Eco31I GGTCTC 1 cut(s) 1958
Eco47I GGWCC 8 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362
Eco47III AGCGCT 1 cut(s) 2696
Eco53kI GAGCTC 2 cut(s) 139, 866
Eco57I CTGAAG 5 cut(s) 539, 624, 1571, 2487, 2840
Eco81I CCTNAGG 1 cut(s) 745
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 2 cut(s) 139, 866
EcoNI CCTNNNNNAGG 1 cut(s) 1098
EcoO109I RGGNCCY 2 cut(s) 1268, 2809
EcoRI GAATTC 1 cut(s) 1336
EcoRII CCWGG 2 cut(s) 1882, 2805
EcoT14I CCWWGG 2 cut(s) 324, 520
EcoT22I ATGCAT 3 cut(s) 619, 666, 996
EcoT38I GRGCYC 4 cut(s) 141, 829, 868, 1409
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 324, 520
FalI AAGNNNNNCTT 6 cut(s) 417, 449, 577, 609, 853, 885
FaqI GGGAC 5 cut(s) 819, 1196, 1524, 1746, 1839
FauI CCCGC 2 cut(s) 342, 398
FauNDI CATATG 1 cut(s) 2533
FbaI TGATCA 3 cut(s) 2419, 2503, 2958
FblI GTMKAC 4 cut(s) 722, 1133, 1326, 2461
FriOI GRGCYC 4 cut(s) 141, 829, 868, 1409
FspBI CTAG 6 cut(s) 606, 773, 822, 831, 1217, 2018
FspI TGCGCA 1 cut(s) 345
GlaI GCGC 5 cut(s) 345, 358, 695, 2696, 2804
GsaI CCCAGC 2 cut(s) 1283, 1368
GsuI CTGGAG 2 cut(s) 1503, 2693
HaeII RGCGCY 3 cut(s) 360, 2698, 2806
HaeIII GGCC 7 cut(s) 164, 519, 1093, 1160, 1293, 2073, 2810
HapII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HgaI GACGC 1 cut(s) 221
HhaI GCGC 5 cut(s) 346, 359, 696, 2697, 2805
Hin1I GRCGYC 2 cut(s) 213, 357
Hin6I GCGC 5 cut(s) 344, 357, 694, 2695, 2803
HinP1I GCGC 5 cut(s) 344, 357, 694, 2695, 2803
HincII GTYRAC 2 cut(s) 220, 2523
HindII GTYRAC 2 cut(s) 220, 2523
HindIII AAGCTT 1 cut(s) 1722
HpaI GTTAAC 1 cut(s) 220
HpaII CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
HphI GGTGA 3 cut(s) 434, 1040, 1380
Hpy166II GTNNAC 7 cut(s) 220, 723, 1134, 1327, 1702, 2462, 2523
Hpy8I GTNNAC 7 cut(s) 220, 723, 1134, 1327, 1702, 2462, 2523
Hpy99I CGWCG 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 2 cut(s) 213, 357
HspAI GCGC 5 cut(s) 344, 357, 694, 2695, 2803
KasI GGCGCC 1 cut(s) 356
KpnI GGTACC 1 cut(s) 2835
Ksp22I TGATCA 3 cut(s) 2419, 2503, 2958
KspAI GTTAAC 1 cut(s) 220
KspI CCGCGG 1 cut(s) 302
LguI GCTCTTC 2 cut(s) 856, 1590
MabI ACCWGGT 1 cut(s) 1882
MaeI CTAG 6 cut(s) 606, 773, 822, 831, 1217, 2018
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 7 cut(s) 4, 1386, 1904, 2380, 2396, 2512, 2815
MflI RGATCY 2 cut(s) 805, 943
MhlI GDGCHC 8 cut(s) 141, 829, 868, 1409, 1585, 1771, 2244, 2568
MlsI TGGCCA 1 cut(s) 1160
MluNI TGGCCA 1 cut(s) 1160
Mly113I GGCGCC 1 cut(s) 357
MlyI GAGTC 3 cut(s) 944, 1472, 2365
MmeI TCCRAC 2 cut(s) 358, 832
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 3 cut(s) 619, 666, 996
MroXI GAANNNNTTC 4 cut(s) 2234, 2853, 2901, 2912
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2888, 2937
MslI CAYNNNNRTG 4 cut(s) 759, 1697, 2532, 2571
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 4 cut(s) 301, 316, 349, 1532
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 7 cut(s) 257, 360, 449, 510, 610, 838, 1575
MspR9I CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
Mva1269I GAATGC 2 cut(s) 619, 1117
MvaI CCWGG 2 cut(s) 1884, 2807
MvnI CGCG 1 cut(s) 301
NarI GGCGCC 1 cut(s) 357
NciI CCSGG 3 cut(s) 510, 511, 838
NdeI CATATG 1 cut(s) 2533
NheI GCTAGC 2 cut(s) 605, 1216
NmeAIII GCCGAG 2 cut(s) 571, 1319
NmuCI GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
NsbI TGCGCA 1 cut(s) 345
NsiI ATGCAT 3 cut(s) 619, 666, 996
NspI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
NspV TTCGAA 1 cut(s) 20
PaeI GCATGC 1 cut(s) 2618
PagI TCATGA 1 cut(s) 1666
PceI AGGCCT 1 cut(s) 1093
PciSI GCTCTTC 2 cut(s) 856, 1590
PctI GAATGC 2 cut(s) 619, 1117
PdmI GAANNNNTTC 4 cut(s) 2234, 2853, 2901, 2912
PfeI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1798, 2224
PflFI GACNNNGTC 1 cut(s) 2518
PflMI CCANNNNNTGG 2 cut(s) 851, 1826
PleI GAGTC 3 cut(s) 943, 1472, 2365
PluTI GGCGCC 1 cut(s) 360
PpsI GAGTC 3 cut(s) 943, 1472, 2365
Ppu21I YACGTR 1 cut(s) 15
PpuMI RGGWCCY 1 cut(s) 1268
Psp124BI GAGCTC 2 cut(s) 141, 868
Psp5II RGGWCCY 1 cut(s) 1268
Psp6I CCWGG 2 cut(s) 1882, 2805
PspFI CCCAGC 2 cut(s) 1279, 1364
PspGI CCWGG 2 cut(s) 1882, 2805
PspPPI RGGWCCY 1 cut(s) 1268
PstI CTGCAG 3 cut(s) 943, 1620, 2254
PstNI CAGNNNCTG 2 cut(s) 1532, 1535
PsuI RGATCY 2 cut(s) 805, 943
PsyI GACNNNGTC 1 cut(s) 2518
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 1 cut(s) 2833
RsaNI GTAC 1 cut(s) 2832
RseI CAYNNNNRTG 4 cut(s) 759, 1697, 2532, 2571
SacI GAGCTC 2 cut(s) 141, 868
SacII CCGCGG 1 cut(s) 302
SapI GCTCTTC 2 cut(s) 856, 1590
SaqAI TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2888, 2937
SchI GAGTC 3 cut(s) 944, 1472, 2365
ScrFI CCNGG 5 cut(s) 510, 511, 838, 1884, 2807
SduI GDGCHC 8 cut(s) 141, 829, 868, 1409, 1585, 1771, 2244, 2568
SexAI ACCWGGT 1 cut(s) 1882
SfcI CTRYAG 4 cut(s) 939, 1616, 2250, 2343
SfoI GGCGCC 1 cut(s) 358
Sfr303I CCGCGG 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 302
SinI GGWCC 8 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 4 cut(s) 759, 1697, 2532, 2571
SmlI CTYRAG 3 cut(s) 56, 583, 1261
SmoI CTYRAG 3 cut(s) 56, 583, 1261
SnaBI TACGTA 1 cut(s) 15
SphI GCATGC 1 cut(s) 2618
SseBI AGGCCT 1 cut(s) 1093
SspDI GGCGCC 1 cut(s) 356
SspI AATATT 1 cut(s) 2972
SspMI CTAG 6 cut(s) 606, 773, 822, 831, 1217, 2018
SstI GAGCTC 2 cut(s) 141, 868
StuI AGGCCT 1 cut(s) 1093
StyD4I CCNGG 5 cut(s) 508, 509, 836, 1882, 2805
StyI CCWWGG 2 cut(s) 324, 520
TaiI ACGT 1 cut(s) 17
TaqI TCGA 4 cut(s) 20, 1992, 2238, 2326
TauI GCSGC 6 cut(s) 304, 313, 316, 357, 464, 467
TfiI GAWTC 8 cut(s) 97, 550, 841, 1003, 1021, 1097, 1798, 2224
Tru1I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2888, 2937
Tru9I TTAA 9 cut(s) 57, 219, 1068, 1227, 1287, 1625, 2780, 2888, 2937
TseFI GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
Tsp45I GTSAC 5 cut(s) 1386, 1904, 2380, 2396, 2512
TspMI CCCGGG 1 cut(s) 509
Tth111I GACNNNGTC 1 cut(s) 2518
Van91I CCANNNNNTGG 2 cut(s) 851, 1826
Vha464I CTTAAG 1 cut(s) 56
VpaK11BI GGWCC 8 cut(s) 321, 1183, 1268, 1511, 1603, 1880, 2095, 2362
XagI CCTNNNNNAGG 1 cut(s) 1098
XapI RAATTY 8 cut(s) 901, 1075, 1336, 1516, 1987, 2064, 2556, 2750
XceI RCATGY 4 cut(s) 1696, 2294, 2618, 2669
XmaI CCCGGG 1 cut(s) 509
XmiI GTMKAC 4 cut(s) 722, 1133, 1326, 2461
XmnI GAANNNNTTC 4 cut(s) 2234, 2853, 2901, 2912
XspI CTAG 6 cut(s) 606, 773, 822, 831, 1217, 2018
Zsp2I ATGCAT 3 cut(s) 619, 666, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.