RLG00000035840

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002776
Physical Location & Seq
Forward (+)
72512233 .. 72519146
6914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035840

Sequence Viewer

Length: 3255 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTCAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGGTGGCGGTGGAGGTGGAGGTGGAGGAGGGGGTGTTGGCGGTGGTGGGGTTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGAAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGAGGCTTCCGCCTCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCTGGTTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCAGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCGTCGTGACTTGATGACATCTGATGCTGTACGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAAATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATCAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCAGCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCATTTGCCATCACAGATCAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCTCCACAGTCGGCAAAAAGTTCGTACTCGGATTTGGGTAAGAGCAATGGGGTTGGGTCAGATCTGAACAACTCATCCCCTGATAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTACATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGAGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACTGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGTGTTGGTGTTCCCTTGGGGGGTAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCCGTTATTCCCAACTCTCCAGTTGGCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATCCAGCTGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCGGAAATCGTAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCATGGACTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTCGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGCATTGAATGTGTCCCTGAAGACGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAGCGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTATGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1085

Amino Acids

115.9

Weight (kDa)

5.83

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 319 - 746 3.6e-172 Nucleic acid binding protein NABP
PUF PF00806 751 - 785 2.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 789 - 820 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 823 - 856 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 859 - 938 2e-07 NOP9-like PUF repeat domain
PUF PF00806 895 - 930 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 935 - 965 8.4e-10 Pumilio-family RNA binding repeat
PUF PF00806 969 - 1002 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1014 - 1043 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2576
Acc65I GGTACC 1 cut(s) 1606
AccB1I GGYRCC 3 cut(s) 157, 1606, 1735
AccB7I CCANNNNNTGG 2 cut(s) 32, 868
AccII CGCG 1 cut(s) 3108
AciI CCGC 9 cut(s) 161, 188, 210, 216, 249, 392, 533, 1718, 2270
AclI AACGTT 1 cut(s) 2672
AclWI GGATC 9 cut(s) 332, 510, 932, 1414, 1742, 1808, 2144, 2157, 2280
AcoI YGGCCR 3 cut(s) 841, 2448, 2572
AcsI RAATTY 7 cut(s) 62, 1102, 1474, 1855, 2107, 2113, 2261
AcuI CTGAAG 4 cut(s) 795, 990, 2664, 3017
AcyI GRCGYC 3 cut(s) 158, 1736, 2649
AfaI GTAC 8 cut(s) 694, 825, 1295, 1555, 1608, 1632, 2847, 3072
AfiI CCNNNNNNNGG 8 cut(s) 32, 635, 707, 868, 986, 1353, 1718, 1937
AflIII ACRYGT 1 cut(s) 2251
AhlI ACTAGT 1 cut(s) 2989
AjuI GAANNNNNNNTTGG 4 cut(s) 1508, 1540, 2099, 2131
AloI GAACNNNNNNTCC 2 cut(s) 1328, 1360
Alw21I GWGCWC 2 cut(s) 1137, 2745
Alw26I GTCTC 5 cut(s) 1054, 1733, 2168, 2671, 3080
AlwI GGATC 9 cut(s) 332, 510, 932, 1414, 1742, 1808, 2144, 2157, 2280
AlwNI CAGNNNCTG 1 cut(s) 1673
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 6 cut(s) 23, 841, 871, 2047, 2448, 2572
ApaI GGGCCC 1 cut(s) 875
ApoI RAATTY 7 cut(s) 62, 1102, 1474, 1855, 2107, 2113, 2261
Asp700I GAANNNNTTC 2 cut(s) 672, 1621
Asp718I GGTACC 1 cut(s) 1606
AspLEI GCGC 4 cut(s) 160, 1738, 2586, 3108
AspS9I GGNCC 7 cut(s) 871, 872, 1655, 2048, 2135, 2539, 2969
AsuHPI GGTGA 4 cut(s) 1166, 1890, 2518, 3236
AsuNHI GCTAGC 1 cut(s) 1541
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 4 cut(s) 1655, 2135, 2539, 2969
BaeGI GKGCMC 1 cut(s) 875
BalI TGGCCA 2 cut(s) 843, 2450
BamHI GGATCC 1 cut(s) 2149
BanI GGYRCC 3 cut(s) 157, 1606, 1735
BanII GRGCYC 2 cut(s) 875, 961
BauI CACGAG 1 cut(s) 3183
BbsI GAAGAC 2 cut(s) 1101, 2652
Bbv12I GWGCWC 2 cut(s) 1137, 2745
BbvCI CCTCAGC 1 cut(s) 329
BccI CCATC 3 cut(s) 605, 1153, 2437
BceAI ACGGC 2 cut(s) 717, 2587
BcgI CGANNNNNNTGC 2 cut(s) 1272, 1306
BclI TGATCA 2 cut(s) 1171, 2680
BcoDI GTCTC 5 cut(s) 1054, 1733, 2168, 2671, 3080
BcuI ACTAGT 1 cut(s) 2989
BfaI CTAG 9 cut(s) 504, 905, 954, 963, 1026, 1389, 1542, 1664, 2990
BfmI CTRYAG 4 cut(s) 855, 897, 1590, 1788
BfoI RGCGCY 2 cut(s) 161, 1739
BglII AGATCT 2 cut(s) 1075, 1330
BlpI GCTNAGC 3 cut(s) 592, 1218, 2956
BmcAI AGTACT 1 cut(s) 1632
Bme18I GGWCC 4 cut(s) 1655, 2135, 2539, 2969
BmeT110I CYCGRG 1 cut(s) 547
BmgT120I GGNCC 7 cut(s) 871, 872, 1655, 2048, 2135, 2539, 2969
BmtI GCTAGC 1 cut(s) 1545
BpiI GAAGAC 2 cut(s) 1101, 2652
BpmI CTGGAG 5 cut(s) 309, 1092, 1715, 2025, 2870
Bpu10I CCTNAGC 2 cut(s) 89, 329
Bpu1102I GCTNAGC 3 cut(s) 592, 1218, 2956
BpuEI CTTGAG 3 cut(s) 1789, 2343, 2759
BsaHI GRCGYC 3 cut(s) 158, 1736, 2649
BsaI GGTCTC 2 cut(s) 1733, 2168
BsaJI CCNNGG 9 cut(s) 383, 707, 844, 1524, 1533, 1932, 2013, 2050, 2138
BsaXI ACNNNNNCTCC 8 cut(s) 228, 258, 317, 347, 981, 1011, 1065, 1095
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 635, 707, 868, 986, 1353, 1718, 1937
Bse1I ACTGG 5 cut(s) 1561, 1729, 1951, 2042, 2853
Bse3DI GCAATG 3 cut(s) 1321, 2755, 3045
BseDI CCNNGG 9 cut(s) 383, 707, 844, 1524, 1533, 1932, 2013, 2050, 2138
BseLI CCNNNNNNNGG 8 cut(s) 32, 635, 707, 868, 986, 1353, 1718, 1937
BseMI GCAATG 3 cut(s) 1321, 2755, 3045
BseMII CTCAG 6 cut(s) 103, 320, 606, 1916, 2777, 3245
BseNI ACTGG 5 cut(s) 1561, 1729, 1951, 2042, 2853
BseRI GAGGAG 7 cut(s) 249, 335, 338, 1002, 1560, 1653, 3231
BseSI GKGCMC 1 cut(s) 875
BseYI CCCAGC 3 cut(s) 263, 2897, 3206
Bsh1236I CGCG 1 cut(s) 3108
BshFI GGCC 6 cut(s) 25, 843, 873, 2049, 2450, 2574
BshNI GGYRCC 3 cut(s) 157, 1606, 1735
BsiHKAI GWGCWC 2 cut(s) 1137, 2745
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 1 cut(s) 575
BsiWI CGTACG 1 cut(s) 692
BslFI GGGAC 7 cut(s) 485, 774, 1735, 1995, 2007, 2148, 2624
BslI CCNNNNNNNGG 8 cut(s) 32, 635, 707, 868, 986, 1353, 1718, 1937
BsmAI GTCTC 5 cut(s) 1054, 1733, 2168, 2671, 3080
BsmFI GGGAC 7 cut(s) 485, 774, 1735, 1995, 2007, 2148, 2624
BsmI GAATGC 2 cut(s) 190, 3148
BsnI GGCC 6 cut(s) 25, 843, 873, 2049, 2450, 2574
Bso31I GGTCTC 2 cut(s) 1733, 2168
BsoBI CYCGRG 1 cut(s) 547
Bsp120I GGGCCC 1 cut(s) 871
Bsp1286I GDGCHC 4 cut(s) 875, 961, 1137, 2745
Bsp1407I TGTACA 1 cut(s) 3070
Bsp1720I GCTNAGC 3 cut(s) 592, 1218, 2956
Bsp19I CCATGG 3 cut(s) 1533, 2013, 2138
BspACI CCGC 9 cut(s) 161, 188, 210, 216, 249, 392, 533, 1718, 2270
BspANI GGCC 6 cut(s) 25, 843, 873, 2049, 2450, 2574
BspCNI CTCAG 6 cut(s) 102, 321, 605, 1915, 2776, 3244
BspFNI CGCG 1 cut(s) 3108
BspMAI CTGCAG 4 cut(s) 859, 901, 1594, 1792
BspOI GCTAGC 1 cut(s) 1545
BspPI GGATC 9 cut(s) 332, 510, 932, 1414, 1742, 1808, 2144, 2157, 2280
BspT107I GGYRCC 3 cut(s) 157, 1606, 1735
BspTNI GGTCTC 2 cut(s) 1733, 2168
BsrDI GCAATG 3 cut(s) 1321, 2755, 3045
BsrGI TGTACA 1 cut(s) 3070
BsrI ACTGG 5 cut(s) 1561, 1729, 1951, 2042, 2853
BssECI CCNNGG 9 cut(s) 383, 707, 844, 1524, 1533, 1932, 2013, 2050, 2138
BssNI GRCGYC 3 cut(s) 158, 1736, 2649
BssSI CACGAG 1 cut(s) 3183
BssT1I CCWWGG 5 cut(s) 1524, 1533, 1932, 2013, 2138
Bst2BI CACGAG 1 cut(s) 3183
Bst4CI ACNGT 3 cut(s) 169, 1278, 1914
Bst6I CTCTTC 3 cut(s) 988, 2193, 3036
BstACI GRCGYC 3 cut(s) 158, 1736, 2649
BstAPI GCANNNNNTGC 3 cut(s) 1141, 1673, 1799
BstAUI TGTACA 1 cut(s) 3070
BstC8I GCNNGC 7 cut(s) 208, 730, 1543, 1800, 2804, 2808, 3110
BstDSI CCRYGG 3 cut(s) 1533, 2013, 2138
BstENI CCTNNNNNAGG 1 cut(s) 984
BstFNI CGCG 1 cut(s) 3108
BstH2I RGCGCY 2 cut(s) 161, 1739
BstHHI GCGC 4 cut(s) 160, 1738, 2586, 3108
BstMAI GTCTC 5 cut(s) 1054, 1733, 2168, 2671, 3080
BstNSI RCATGY 5 cut(s) 853, 1996, 2255, 2806, 2846
BstSFI CTRYAG 4 cut(s) 855, 897, 1590, 1788
BstSLI GKGCMC 1 cut(s) 875
BstUI CGCG 1 cut(s) 3108
BstV2I GAAGAC 2 cut(s) 1101, 2652
BstX2I RGATCY 4 cut(s) 515, 1075, 1330, 2149
BstYI RGATCY 4 cut(s) 515, 1075, 1330, 2149
BsuRI GGCC 6 cut(s) 25, 843, 873, 2049, 2450, 2574
BtgI CCRYGG 3 cut(s) 1533, 2013, 2138
BtsI GCAGTG 1 cut(s) 2743
BtsIMutI CAGTG 2 cut(s) 2272, 2743
Cac8I GCNNGC 7 cut(s) 208, 730, 1543, 1800, 2804, 2808, 3110
CaiI CAGNNNCTG 1 cut(s) 1673
CfoI GCGC 4 cut(s) 160, 1738, 2586, 3108
Cfr13I GGNCC 7 cut(s) 871, 872, 1655, 2048, 2135, 2539, 2969
CseI GACGC 4 cut(s) 89, 303, 785, 2657
Csp6I GTAC 8 cut(s) 693, 824, 1294, 1554, 1607, 1631, 2846, 3071
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviQI GTAC 8 cut(s) 693, 824, 1294, 1554, 1607, 1631, 2846, 3071
DinI GGCGCC 2 cut(s) 159, 1737
DrdI GACNNNNNNGTC 1 cut(s) 2576
DseDI GACNNNNNNGTC 1 cut(s) 2576
EaeI YGGCCR 3 cut(s) 841, 2448, 2572
Eam1104I CTCTTC 3 cut(s) 988, 2193, 3036
EarI CTCTTC 3 cut(s) 988, 2193, 3036
EciI GGCGGA 1 cut(s) 381
Eco130I CCWWGG 5 cut(s) 1524, 1533, 1932, 2013, 2138
Eco24I GRGCYC 2 cut(s) 875, 961
Eco31I GGTCTC 2 cut(s) 1733, 2168
Eco47I GGWCC 4 cut(s) 1655, 2135, 2539, 2969
Eco57I CTGAAG 4 cut(s) 795, 990, 2664, 3017
Eco88I CYCGRG 1 cut(s) 547
EcoNI CCTNNNNNAGG 1 cut(s) 984
EcoRI GAATTC 1 cut(s) 2261
EcoT14I CCWWGG 5 cut(s) 1524, 1533, 1932, 2013, 2138
EcoT22I ATGCAT 2 cut(s) 742, 3148
EcoT38I GRGCYC 2 cut(s) 875, 961
EgeI GGCGCC 2 cut(s) 159, 1737
EheI GGCGCC 2 cut(s) 159, 1737
ErhI CCWWGG 5 cut(s) 1524, 1533, 1932, 2013, 2138
FaqI GGGAC 7 cut(s) 485, 774, 1735, 1995, 2007, 2148, 2624
FauI CCCGC 1 cut(s) 1711
FauNDI CATATG 2 cut(s) 1263, 2710
FbaI TGATCA 2 cut(s) 1171, 2680
FriOI GRGCYC 2 cut(s) 875, 961
FspBI CTAG 9 cut(s) 504, 905, 954, 963, 1026, 1389, 1542, 1664, 2990
GlaI GCGC 4 cut(s) 159, 1737, 2585, 3107
GsaI CCCAGC 3 cut(s) 267, 2901, 3210
GsuI CTGGAG 5 cut(s) 309, 1092, 1715, 2025, 2870
HaeII RGCGCY 2 cut(s) 161, 1739
HaeIII GGCC 6 cut(s) 25, 843, 873, 2049, 2450, 2574
HapII CCGG 1 cut(s) 575
HgaI GACGC 4 cut(s) 89, 303, 785, 2657
HhaI GCGC 4 cut(s) 160, 1738, 2586, 3108
Hin1I GRCGYC 3 cut(s) 158, 1736, 2649
Hin6I GCGC 4 cut(s) 158, 1736, 2584, 3106
HinP1I GCGC 4 cut(s) 158, 1736, 2584, 3106
HincII GTYRAC 1 cut(s) 2921
HindII GTYRAC 1 cut(s) 2921
HindIII AAGCTT 1 cut(s) 2456
HpaII CCGG 1 cut(s) 575
HphI GGTGA 4 cut(s) 1166, 1890, 2518, 3236
Hpy166II GTNNAC 3 cut(s) 487, 2341, 2921
Hpy8I GTNNAC 3 cut(s) 487, 2341, 2921
Hpy99I CGWCG 3 cut(s) 105, 319, 801
HpyCH4III ACNGT 3 cut(s) 169, 1278, 1914
HpyCH4IV ACGT 2 cut(s) 2672, 3066
HpySE526I ACGT 2 cut(s) 2672, 3066
Hsp92I GRCGYC 3 cut(s) 158, 1736, 2649
HspAI GCGC 4 cut(s) 158, 1736, 2584, 3106
KasI GGCGCC 2 cut(s) 157, 1735
KpnI GGTACC 1 cut(s) 1610
Ksp22I TGATCA 2 cut(s) 1171, 2680
MaeI CTAG 9 cut(s) 504, 905, 954, 963, 1026, 1389, 1542, 1664, 2990
MaeII ACGT 2 cut(s) 2672, 3066
MaeIII GTNAC 9 cut(s) 38, 128, 800, 1391, 1896, 1908, 2162, 2594, 2689
MflI RGATCY 4 cut(s) 515, 1075, 1330, 2149
MhlI GDGCHC 4 cut(s) 875, 961, 1137, 2745
MlsI TGGCCA 2 cut(s) 843, 2450
MluNI TGGCCA 2 cut(s) 843, 2450
Mly113I GGCGCC 2 cut(s) 158, 1736
MlyI GAGTC 5 cut(s) 691, 1076, 1717, 2293, 2926
MmeI TCCRAC 2 cut(s) 297, 3063
Mox20I TGGCCA 2 cut(s) 843, 2450
Mph1103I ATGCAT 2 cut(s) 742, 3148
MroXI GAANNNNTTC 2 cut(s) 672, 1621
MscI TGGCCA 2 cut(s) 843, 2450
MseI TTAA 8 cut(s) 543, 1041, 1235, 1425, 1499, 2079, 2205, 3129
MslI CAYNNNNRTG 4 cut(s) 2709, 2713, 2748, 3141
Msp20I TGGCCA 2 cut(s) 843, 2450
MspA1I CMGCKG 2 cut(s) 1670, 2156
MspI CCGG 1 cut(s) 575
Mva1269I GAATGC 2 cut(s) 190, 3148
MvnI CGCG 1 cut(s) 3108
NarI GGCGCC 2 cut(s) 158, 1736
NcoI CCATGG 3 cut(s) 1533, 2013, 2138
NdeI CATATG 2 cut(s) 1263, 2710
NheI GCTAGC 1 cut(s) 1541
NmuCI GTSAC 6 cut(s) 128, 800, 1896, 1908, 2594, 2689
NsiI ATGCAT 2 cut(s) 742, 3148
NspI RCATGY 5 cut(s) 853, 1996, 2255, 2806, 2846
PaeI GCATGC 1 cut(s) 2806
PaeR7I CTCGAG 1 cut(s) 547
PciI ACATGT 1 cut(s) 2251
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3148
PdmI GAANNNNTTC 2 cut(s) 672, 1621
PfeI GAWTC 9 cut(s) 373, 379, 1247, 1420, 1516, 1622, 1682, 2401, 3115
Pfl23II CGTACG 1 cut(s) 692
PflMI CCANNNNNTGG 2 cut(s) 32, 868
PfoI TCCNGGA 3 cut(s) 286, 1612, 2002
PleI GAGTC 5 cut(s) 691, 1075, 1716, 2292, 2925
PluTI GGCGCC 2 cut(s) 161, 1739
PpsI GAGTC 5 cut(s) 691, 1075, 1716, 2292, 2925
PscI ACATGT 1 cut(s) 2251
Psp1406I AACGTT 1 cut(s) 2672
PspFI CCCAGC 3 cut(s) 263, 2897, 3206
PspLI CGTACG 1 cut(s) 692
PspOMI GGGCCC 1 cut(s) 871
PspPI GGNCC 7 cut(s) 871, 872, 1655, 2048, 2135, 2539, 2969
PspXI VCTCGAGB 1 cut(s) 547
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 859, 901, 1594, 1792
PstNI CAGNNNCTG 1 cut(s) 1673
PsuI RGATCY 4 cut(s) 515, 1075, 1330, 2149
PvuII CAGCTG 2 cut(s) 1670, 2156
RsaI GTAC 8 cut(s) 694, 825, 1295, 1555, 1608, 1632, 2847, 3072
RsaNI GTAC 8 cut(s) 693, 824, 1294, 1554, 1607, 1631, 2846, 3071
RseI CAYNNNNRTG 4 cut(s) 2709, 2713, 2748, 3141
SaqAI TTAA 8 cut(s) 543, 1041, 1235, 1425, 1499, 2079, 2205, 3129
Sau96I GGNCC 7 cut(s) 871, 872, 1655, 2048, 2135, 2539, 2969
ScaI AGTACT 1 cut(s) 1632
SchI GAGTC 5 cut(s) 691, 1076, 1717, 2293, 2926
SduI GDGCHC 4 cut(s) 875, 961, 1137, 2745
SfcI CTRYAG 4 cut(s) 855, 897, 1590, 1788
SfoI GGCGCC 2 cut(s) 159, 1737
Sfr274I CTCGAG 1 cut(s) 547
SinI GGWCC 4 cut(s) 1655, 2135, 2539, 2969
SlaI CTCGAG 1 cut(s) 547
SmiMI CAYNNNNRTG 4 cut(s) 2709, 2713, 2748, 3141
SmlI CTYRAG 4 cut(s) 547, 1768, 2358, 2738
SmoI CTYRAG 4 cut(s) 547, 1768, 2358, 2738
SpeI ACTAGT 1 cut(s) 2989
SphI GCATGC 1 cut(s) 2806
SsiI CCGC 9 cut(s) 161, 188, 210, 216, 249, 392, 533, 1718, 2270
SspDI GGCGCC 2 cut(s) 157, 1735
SspI AATATT 1 cut(s) 139
SspMI CTAG 9 cut(s) 504, 905, 954, 963, 1026, 1389, 1542, 1664, 2990
StyI CCWWGG 5 cut(s) 1524, 1533, 1932, 2013, 2138
TaaI ACNGT 3 cut(s) 169, 1278, 1914
TaiI ACGT 2 cut(s) 2675, 3069
TaqI TCGA 6 cut(s) 365, 407, 527, 548, 1418, 2559
TatI WGTACW 3 cut(s) 1630, 2845, 3070
TauI GCSGC 2 cut(s) 163, 535
TfiI GAWTC 9 cut(s) 373, 379, 1247, 1420, 1516, 1622, 1682, 2401, 3115
Tru1I TTAA 8 cut(s) 543, 1041, 1235, 1425, 1499, 2079, 2205, 3129
Tru9I TTAA 8 cut(s) 543, 1041, 1235, 1425, 1499, 2079, 2205, 3129
TscAI CASTG 2 cut(s) 2272, 2750
TseFI GTSAC 6 cut(s) 128, 800, 1896, 1908, 2594, 2689
Tsp45I GTSAC 6 cut(s) 128, 800, 1896, 1908, 2594, 2689
TspGWI ACGGA 1 cut(s) 2336
TspRI CASTG 2 cut(s) 2272, 2750
Van91I CCANNNNNTGG 2 cut(s) 32, 868
VpaK11BI GGWCC 4 cut(s) 1655, 2135, 2539, 2969
XagI CCTNNNNNAGG 1 cut(s) 984
XapI RAATTY 7 cut(s) 62, 1102, 1474, 1855, 2107, 2113, 2261
XceI RCATGY 5 cut(s) 853, 1996, 2255, 2806, 2846
XcmI CCANNNNNNNNNTGG 1 cut(s) 1553
XhoI CTCGAG 1 cut(s) 547
XmnI GAANNNNTTC 2 cut(s) 672, 1621
XspI CTAG 9 cut(s) 504, 905, 954, 963, 1026, 1389, 1542, 1664, 2990
ZrmI AGTACT 1 cut(s) 1632
Zsp2I ATGCAT 2 cut(s) 742, 3148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.