Rroxscaffold_1G00014920

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002776
Physical Location & Seq
Reverse (-)
18508896 .. 18515664
6769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014920.1

Sequence Viewer

Length: 2973 bp
ATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGCTGGCGGTGGAAGTGGAGGTGGAGGAGGGGGTGTTGGCGGTGGTGGGGTTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGCGGCTTCCGCCTCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCAGGGTTTAACTCGAGGAAGCAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACAACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCGCCGTGACTTGATGACATCTGATGCTCTACGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAAATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAACTCCTTTAATGCTGTCTCATCTGGCATAAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCAGCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCATTTGCCATCACAGAACAAACATGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCATACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCTCCACAGTCGGCAAAAGGTTCGTACTCAGATTTGGGAAAGAGCAATGGTGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGCAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGAGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACTGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGAAGTCCCATGGCAAGTCCCGTTATTCCCAACTCTCCAGTTGGCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATCTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATGCAGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCGGAAATTAGAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCACTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGACTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTCGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAGTTACTGGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

990

Amino Acids

105.86

Weight (kDa)

5.7

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 310 - 469 1.3e-44 Nucleic acid binding protein NABP
NABP PF07990 470 - 577 1.3e-36 Nucleic acid binding protein NABP
NABP PF07990 574 - 652 1.9e-35 Nucleic acid binding protein NABP
PUF PF00806 657 - 691 2.6e-10 Pumilio-family RNA binding repeat
PUF PF00806 695 - 726 1.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 729 - 762 1.7e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 765 - 845 1.6e-07 NOP9-like PUF repeat domain
PUF PF00806 771 - 795 9.4e-06 Pumilio-family RNA binding repeat
PUF PF00806 801 - 836 6.6e-13 Pumilio-family RNA binding repeat
PUF PF00806 841 - 871 7.6e-10 Pumilio-family RNA binding repeat
PUF PF00806 875 - 908 3.8e-07 Pumilio-family RNA binding repeat
PUF PF00806 920 - 949 1.8e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2294
Acc36I ACCTGC 1 cut(s) 1865
Acc65I GGTACC 1 cut(s) 1492
AccB1I GGYRCC 3 cut(s) 130, 1492, 1621
AccB7I CCANNNNNTGG 1 cut(s) 841
AccII CGCG 2 cut(s) 358, 2826
AclI AACGTT 1 cut(s) 2390
AclWI GGATC 7 cut(s) 305, 483, 905, 1387, 1628, 1694, 1998
AcoI YGGCCR 3 cut(s) 814, 2166, 2290
AcsI RAATTY 5 cut(s) 35, 1075, 1828, 1834, 1979
AcuI CTGAAG 4 cut(s) 768, 963, 2382, 2735
AcyI GRCGYC 2 cut(s) 131, 1622
AfaI GTAC 7 cut(s) 667, 1268, 1441, 1494, 1518, 2565, 2790
AfiI CCNNNNNNNGG 7 cut(s) 608, 680, 841, 959, 1326, 1604, 2365
AflIII ACRYGT 1 cut(s) 1969
AjuI GAANNNNNNNTTGG 2 cut(s) 1820, 1852
AloI GAACNNNNNNTCC 2 cut(s) 1301, 1333
Alw21I GWGCWC 3 cut(s) 1110, 2139, 2463
Alw26I GTCTC 5 cut(s) 1027, 1619, 1886, 2389, 2798
AlwI GGATC 7 cut(s) 305, 483, 905, 1387, 1628, 1694, 1998
AlwNI CAGNNNCTG 1 cut(s) 1559
Ama87I CYCGRG 1 cut(s) 520
AoxI GGCC 6 cut(s) 814, 844, 1768, 2166, 2290, 2367
ApaI GGGCCC 1 cut(s) 848
ApoI RAATTY 5 cut(s) 35, 1075, 1828, 1834, 1979
Asp700I GAANNNNTTC 2 cut(s) 645, 1507
Asp718I GGTACC 1 cut(s) 1492
AspLEI GCGC 5 cut(s) 133, 185, 771, 1624, 2304
AspS9I GGNCC 7 cut(s) 844, 845, 1541, 1769, 1856, 2257, 2687
AsuHPI GGTGA 4 cut(s) 1139, 2236, 2722, 2954
AsuNHI GCTAGC 1 cut(s) 1427
AvaI CYCGRG 1 cut(s) 520
AvaII GGWCC 4 cut(s) 1541, 1856, 2257, 2687
BaeGI GKGCMC 1 cut(s) 848
BalI TGGCCA 2 cut(s) 816, 2168
BanI GGYRCC 3 cut(s) 130, 1492, 1621
BanII GRGCYC 2 cut(s) 848, 934
BauI CACGAG 1 cut(s) 2901
BbsI GAAGAC 1 cut(s) 1074
Bbv12I GWGCWC 3 cut(s) 1110, 2139, 2463
BbvCI CCTCAGC 1 cut(s) 302
BccI CCATC 3 cut(s) 578, 1126, 2155
BceAI ACGGC 3 cut(s) 690, 756, 2305
BcgI CGANNNNNNTGC 2 cut(s) 1245, 1279
BclI TGATCA 2 cut(s) 1144, 2398
BcoDI GTCTC 5 cut(s) 1027, 1619, 1886, 2389, 2798
BfaI CTAG 8 cut(s) 477, 878, 927, 936, 999, 1362, 1428, 1550
BfmI CTRYAG 4 cut(s) 828, 870, 1476, 1674
BfoI RGCGCY 3 cut(s) 134, 186, 1625
BfuAI ACCTGC 1 cut(s) 1865
BglII AGATCT 2 cut(s) 1048, 1303
BlpI GCTNAGC 3 cut(s) 565, 1191, 2674
BmcAI AGTACT 1 cut(s) 1518
Bme18I GGWCC 4 cut(s) 1541, 1856, 2257, 2687
BmeT110I CYCGRG 1 cut(s) 520
BmgT120I GGNCC 7 cut(s) 844, 845, 1541, 1769, 1856, 2257, 2687
BmtI GCTAGC 1 cut(s) 1431
BpiI GAAGAC 1 cut(s) 1074
BpmI CTGGAG 5 cut(s) 282, 1065, 1601, 1746, 2588
Bpu10I CCTNAGC 2 cut(s) 62, 302
Bpu1102I GCTNAGC 3 cut(s) 565, 1191, 2674
BpuEI CTTGAG 3 cut(s) 1675, 2061, 2477
BsaHI GRCGYC 2 cut(s) 131, 1622
BsaI GGTCTC 2 cut(s) 1619, 1886
BsaJI CCNNGG 9 cut(s) 356, 509, 680, 817, 1410, 1419, 1734, 1771, 1859
BsaXI ACNNNNNCTCC 8 cut(s) 201, 231, 290, 320, 954, 984, 1038, 1068
Bsc4I CCNNNNNNNGG 7 cut(s) 608, 680, 841, 959, 1326, 1604, 2365
Bse1I ACTGG 5 cut(s) 1447, 1615, 1763, 2571, 2712
Bse3DI GCAATG 3 cut(s) 1294, 2473, 2763
BseDI CCNNGG 9 cut(s) 356, 509, 680, 817, 1410, 1419, 1734, 1771, 1859
BseLI CCNNNNNNNGG 7 cut(s) 608, 680, 841, 959, 1326, 1604, 2365
BseMI GCAATG 3 cut(s) 1294, 2473, 2763
BseMII CTCAG 5 cut(s) 293, 579, 1284, 2495, 2963
BseNI ACTGG 5 cut(s) 1447, 1615, 1763, 2571, 2712
BseRI GAGGAG 7 cut(s) 222, 308, 311, 975, 1446, 1539, 2949
BseSI GKGCMC 1 cut(s) 848
BseYI CCCAGC 3 cut(s) 236, 2615, 2924
Bsh1236I CGCG 2 cut(s) 358, 2826
BshFI GGCC 6 cut(s) 816, 846, 1770, 2168, 2292, 2369
BshNI GGYRCC 3 cut(s) 130, 1492, 1621
BsiHKAI GWGCWC 3 cut(s) 1110, 2139, 2463
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 7 cut(s) 458, 747, 1621, 1716, 1728, 1869, 2342
BslI CCNNNNNNNGG 7 cut(s) 608, 680, 841, 959, 1326, 1604, 2365
BsmAI GTCTC 5 cut(s) 1027, 1619, 1886, 2389, 2798
BsmFI GGGAC 7 cut(s) 458, 747, 1621, 1716, 1728, 1869, 2342
BsmI GAATGC 2 cut(s) 163, 2866
BsnI GGCC 6 cut(s) 816, 846, 1770, 2168, 2292, 2369
Bso31I GGTCTC 2 cut(s) 1619, 1886
BsoBI CYCGRG 1 cut(s) 520
Bsp120I GGGCCC 1 cut(s) 844
Bsp1286I GDGCHC 5 cut(s) 848, 934, 1110, 2139, 2463
Bsp1407I TGTACA 1 cut(s) 2788
Bsp1720I GCTNAGC 3 cut(s) 565, 1191, 2674
Bsp19I CCATGG 3 cut(s) 1419, 1734, 1859
BspANI GGCC 6 cut(s) 816, 846, 1770, 2168, 2292, 2369
BspCNI CTCAG 5 cut(s) 294, 578, 1283, 2494, 2962
BspFNI CGCG 2 cut(s) 358, 2826
BspMAI CTGCAG 4 cut(s) 832, 874, 1480, 1678
BspMI ACCTGC 1 cut(s) 1865
BspOI GCTAGC 1 cut(s) 1431
BspPI GGATC 7 cut(s) 305, 483, 905, 1387, 1628, 1694, 1998
BspT107I GGYRCC 3 cut(s) 130, 1492, 1621
BspTNI GGTCTC 2 cut(s) 1619, 1886
BsrDI GCAATG 3 cut(s) 1294, 2473, 2763
BsrGI TGTACA 1 cut(s) 2788
BsrI ACTGG 5 cut(s) 1447, 1615, 1763, 2571, 2712
BssECI CCNNGG 9 cut(s) 356, 509, 680, 817, 1410, 1419, 1734, 1771, 1859
BssNI GRCGYC 2 cut(s) 131, 1622
BssSI CACGAG 1 cut(s) 2901
BssT1I CCWWGG 4 cut(s) 1410, 1419, 1734, 1859
Bst2BI CACGAG 1 cut(s) 2901
Bst4CI ACNGT 2 cut(s) 142, 1251
Bst6I CTCTTC 4 cut(s) 961, 1911, 2358, 2754
BstACI GRCGYC 2 cut(s) 131, 1622
BstAPI GCANNNNNTGC 3 cut(s) 1114, 1559, 1685
BstAUI TGTACA 1 cut(s) 2788
BstC8I GCNNGC 8 cut(s) 181, 187, 703, 1429, 1686, 2522, 2526, 2828
BstDSI CCRYGG 4 cut(s) 356, 1419, 1734, 1859
BstENI CCTNNNNNAGG 1 cut(s) 957
BstFNI CGCG 2 cut(s) 358, 2826
BstH2I RGCGCY 3 cut(s) 134, 186, 1625
BstHHI GCGC 5 cut(s) 133, 185, 771, 1624, 2304
BstMAI GTCTC 5 cut(s) 1027, 1619, 1886, 2389, 2798
BstNSI RCATGY 4 cut(s) 826, 1973, 2524, 2564
BstSFI CTRYAG 4 cut(s) 828, 870, 1476, 1674
BstSLI GKGCMC 1 cut(s) 848
BstUI CGCG 2 cut(s) 358, 2826
BstV2I GAAGAC 1 cut(s) 1074
BstX2I RGATCY 3 cut(s) 488, 1048, 1303
BstYI RGATCY 3 cut(s) 488, 1048, 1303
BsuRI GGCC 6 cut(s) 816, 846, 1770, 2168, 2292, 2369
BtgI CCRYGG 4 cut(s) 356, 1419, 1734, 1859
BtsI GCAGTG 1 cut(s) 2461
BtsIMutI CAGTG 2 cut(s) 1990, 2461
BveI ACCTGC 1 cut(s) 1865
Cac8I GCNNGC 8 cut(s) 181, 187, 703, 1429, 1686, 2522, 2526, 2828
CaiI CAGNNNCTG 1 cut(s) 1559
CfoI GCGC 5 cut(s) 133, 185, 771, 1624, 2304
Cfr13I GGNCC 7 cut(s) 844, 845, 1541, 1769, 1856, 2257, 2687
Cfr42I CCGCGG 1 cut(s) 359
CseI GACGC 2 cut(s) 62, 276
Csp6I GTAC 7 cut(s) 666, 1267, 1440, 1493, 1517, 2564, 2789
CspCI CAANNNNNGTGG 4 cut(s) 541, 576, 1276, 1311
CviQI GTAC 7 cut(s) 666, 1267, 1440, 1493, 1517, 2564, 2789
DinI GGCGCC 2 cut(s) 132, 1623
DrdI GACNNNNNNGTC 1 cut(s) 2294
DseDI GACNNNNNNGTC 1 cut(s) 2294
EaeI YGGCCR 3 cut(s) 814, 2166, 2290
Eam1104I CTCTTC 4 cut(s) 961, 1911, 2358, 2754
EarI CTCTTC 4 cut(s) 961, 1911, 2358, 2754
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 4 cut(s) 1410, 1419, 1734, 1859
Eco24I GRGCYC 2 cut(s) 848, 934
Eco31I GGTCTC 2 cut(s) 1619, 1886
Eco47I GGWCC 4 cut(s) 1541, 1856, 2257, 2687
Eco57I CTGAAG 4 cut(s) 768, 963, 2382, 2735
Eco88I CYCGRG 1 cut(s) 520
EcoNI CCTNNNNNAGG 1 cut(s) 957
EcoRI GAATTC 1 cut(s) 1979
EcoT14I CCWWGG 4 cut(s) 1410, 1419, 1734, 1859
EcoT22I ATGCAT 2 cut(s) 715, 2866
EcoT38I GRGCYC 2 cut(s) 848, 934
EgeI GGCGCC 2 cut(s) 132, 1623
EheI GGCGCC 2 cut(s) 132, 1623
ErhI CCWWGG 4 cut(s) 1410, 1419, 1734, 1859
FaqI GGGAC 7 cut(s) 458, 747, 1621, 1716, 1728, 1869, 2342
FauI CCCGC 1 cut(s) 1597
FauNDI CATATG 2 cut(s) 1236, 2428
FbaI TGATCA 2 cut(s) 1144, 2398
FriOI GRGCYC 2 cut(s) 848, 934
FspBI CTAG 8 cut(s) 477, 878, 927, 936, 999, 1362, 1428, 1550
GlaI GCGC 5 cut(s) 132, 184, 770, 1623, 2303
GsaI CCCAGC 3 cut(s) 240, 2619, 2928
GsuI CTGGAG 5 cut(s) 282, 1065, 1601, 1746, 2588
HaeII RGCGCY 3 cut(s) 134, 186, 1625
HaeIII GGCC 6 cut(s) 816, 846, 1770, 2168, 2292, 2369
HapII CCGG 1 cut(s) 548
HgaI GACGC 2 cut(s) 62, 276
HhaI GCGC 5 cut(s) 133, 185, 771, 1624, 2304
Hin1I GRCGYC 2 cut(s) 131, 1622
Hin6I GCGC 5 cut(s) 131, 183, 769, 1622, 2302
HinP1I GCGC 5 cut(s) 131, 183, 769, 1622, 2302
HincII GTYRAC 1 cut(s) 2639
HindII GTYRAC 1 cut(s) 2639
HindIII AAGCTT 1 cut(s) 2174
HpaII CCGG 1 cut(s) 548
HphI GGTGA 4 cut(s) 1139, 2236, 2722, 2954
Hpy166II GTNNAC 3 cut(s) 460, 2059, 2639
Hpy8I GTNNAC 3 cut(s) 460, 2059, 2639
Hpy99I CGWCG 2 cut(s) 78, 292
HpyCH4III ACNGT 2 cut(s) 142, 1251
HpyCH4IV ACGT 2 cut(s) 2390, 2784
HpySE526I ACGT 2 cut(s) 2390, 2784
Hsp92I GRCGYC 2 cut(s) 131, 1622
HspAI GCGC 5 cut(s) 131, 183, 769, 1622, 2302
KasI GGCGCC 2 cut(s) 130, 1621
KpnI GGTACC 1 cut(s) 1496
Ksp22I TGATCA 2 cut(s) 1144, 2398
KspI CCGCGG 1 cut(s) 359
LmnI GCTCC 9 cut(s) 67, 263, 298, 544, 937, 1406, 2510, 2569, 2962
MaeI CTAG 8 cut(s) 477, 878, 927, 936, 999, 1362, 1428, 1550
MaeII ACGT 2 cut(s) 2390, 2784
MaeIII GTNAC 7 cut(s) 11, 101, 773, 1880, 2312, 2407, 2703
MflI RGATCY 3 cut(s) 488, 1048, 1303
MhlI GDGCHC 5 cut(s) 848, 934, 1110, 2139, 2463
MlsI TGGCCA 2 cut(s) 816, 2168
MluNI TGGCCA 2 cut(s) 816, 2168
Mly113I GGCGCC 2 cut(s) 131, 1622
MlyI GAGTC 4 cut(s) 1049, 1603, 2011, 2644
MmeI TCCRAC 2 cut(s) 270, 2781
Mox20I TGGCCA 2 cut(s) 816, 2168
Mph1103I ATGCAT 2 cut(s) 715, 2866
MroXI GAANNNNTTC 2 cut(s) 645, 1507
MscI TGGCCA 2 cut(s) 816, 2168
MseI TTAA 7 cut(s) 516, 1014, 1208, 1398, 1800, 1923, 2847
MslI CAYNNNNRTG 5 cut(s) 1869, 2427, 2431, 2466, 2859
Msp20I TGGCCA 2 cut(s) 816, 2168
MspA1I CMGCKG 3 cut(s) 358, 569, 1556
MspI CCGG 1 cut(s) 548
Mva1269I GAATGC 2 cut(s) 163, 2866
MvnI CGCG 2 cut(s) 358, 2826
NarI GGCGCC 2 cut(s) 131, 1622
NcoI CCATGG 3 cut(s) 1419, 1734, 1859
NdeI CATATG 2 cut(s) 1236, 2428
NheI GCTAGC 1 cut(s) 1427
NmuCI GTSAC 4 cut(s) 101, 773, 2312, 2407
NsiI ATGCAT 2 cut(s) 715, 2866
NspI RCATGY 4 cut(s) 826, 1973, 2524, 2564
PaeI GCATGC 1 cut(s) 2524
PaeR7I CTCGAG 1 cut(s) 520
PciI ACATGT 1 cut(s) 1969
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PctI GAATGC 2 cut(s) 163, 2866
PdmI GAANNNNTTC 2 cut(s) 645, 1507
PfeI GAWTC 8 cut(s) 346, 352, 1220, 1393, 1508, 1568, 2119, 2833
PflMI CCANNNNNTGG 1 cut(s) 841
PfoI TCCNGGA 2 cut(s) 259, 1498
PleI GAGTC 4 cut(s) 1048, 1602, 2010, 2643
PluTI GGCGCC 2 cut(s) 134, 1625
PpsI GAGTC 4 cut(s) 1048, 1602, 2010, 2643
PscI ACATGT 1 cut(s) 1969
Psp1406I AACGTT 1 cut(s) 2390
PspFI CCCAGC 3 cut(s) 236, 2615, 2924
PspOMI GGGCCC 1 cut(s) 844
PspPI GGNCC 7 cut(s) 844, 845, 1541, 1769, 1856, 2257, 2687
PspXI VCTCGAGB 1 cut(s) 520
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 4 cut(s) 832, 874, 1480, 1678
PstNI CAGNNNCTG 1 cut(s) 1559
PsuI RGATCY 3 cut(s) 488, 1048, 1303
PvuII CAGCTG 1 cut(s) 1556
RsaI GTAC 7 cut(s) 667, 1268, 1441, 1494, 1518, 2565, 2790
RsaNI GTAC 7 cut(s) 666, 1267, 1440, 1493, 1517, 2564, 2789
RseI CAYNNNNRTG 5 cut(s) 1869, 2427, 2431, 2466, 2859
SacII CCGCGG 1 cut(s) 359
SaqAI TTAA 7 cut(s) 516, 1014, 1208, 1398, 1800, 1923, 2847
Sau96I GGNCC 7 cut(s) 844, 845, 1541, 1769, 1856, 2257, 2687
ScaI AGTACT 1 cut(s) 1518
SchI GAGTC 4 cut(s) 1049, 1603, 2011, 2644
SduI GDGCHC 5 cut(s) 848, 934, 1110, 2139, 2463
SfcI CTRYAG 4 cut(s) 828, 870, 1476, 1674
SfoI GGCGCC 2 cut(s) 132, 1623
Sfr274I CTCGAG 1 cut(s) 520
Sfr303I CCGCGG 1 cut(s) 359
SgrBI CCGCGG 1 cut(s) 359
SinI GGWCC 4 cut(s) 1541, 1856, 2257, 2687
SlaI CTCGAG 1 cut(s) 520
SmiMI CAYNNNNRTG 5 cut(s) 1869, 2427, 2431, 2466, 2859
SmlI CTYRAG 4 cut(s) 520, 1654, 2076, 2456
SmoI CTYRAG 4 cut(s) 520, 1654, 2076, 2456
SphI GCATGC 1 cut(s) 2524
SspDI GGCGCC 2 cut(s) 130, 1621
SspI AATATT 1 cut(s) 112
SspMI CTAG 8 cut(s) 477, 878, 927, 936, 999, 1362, 1428, 1550
StyI CCWWGG 4 cut(s) 1410, 1419, 1734, 1859
TaaI ACNGT 2 cut(s) 142, 1251
TaiI ACGT 2 cut(s) 2393, 2787
TaqI TCGA 6 cut(s) 338, 380, 500, 521, 1391, 2277
TatI WGTACW 3 cut(s) 1516, 2563, 2788
TauI GCSGC 3 cut(s) 136, 361, 508
TfiI GAWTC 8 cut(s) 346, 352, 1220, 1393, 1508, 1568, 2119, 2833
Tru1I TTAA 7 cut(s) 516, 1014, 1208, 1398, 1800, 1923, 2847
Tru9I TTAA 7 cut(s) 516, 1014, 1208, 1398, 1800, 1923, 2847
TscAI CASTG 2 cut(s) 1990, 2468
TseFI GTSAC 4 cut(s) 101, 773, 2312, 2407
Tsp45I GTSAC 4 cut(s) 101, 773, 2312, 2407
TspDTI ATGAA 9 cut(s) 48, 425, 735, 846, 1088, 2363, 2751, 2777, 2843
TspGWI ACGGA 2 cut(s) 2054, 2154
TspRI CASTG 2 cut(s) 1990, 2468
Van91I CCANNNNNTGG 1 cut(s) 841
VpaK11BI GGWCC 4 cut(s) 1541, 1856, 2257, 2687
XagI CCTNNNNNAGG 1 cut(s) 957
XapI RAATTY 5 cut(s) 35, 1075, 1828, 1834, 1979
XceI RCATGY 4 cut(s) 826, 1973, 2524, 2564
XcmI CCANNNNNNNNNTGG 1 cut(s) 1439
XhoI CTCGAG 1 cut(s) 520
XmnI GAANNNNTTC 2 cut(s) 645, 1507
XspI CTAG 8 cut(s) 477, 878, 927, 936, 999, 1362, 1428, 1550
ZrmI AGTACT 1 cut(s) 1518
Zsp2I ATGCAT 2 cut(s) 715, 2866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.