MD01G1136900.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002776
Physical Location & Seq
Forward (+)
24613607 .. 24621191
7585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1136900.v1.1.491

Sequence Viewer

Length: 3084 bp
ATGGTCACCGATGCTTATTCAAAGATGATGTCTGAAATGTCAATGCGATCGATGTCAAAGAGTGGCGATTACAGCGAAGATTTGAGTACGTTGATTCGTGAACAGAGGCGGCAGCATGAAGCAAGTGAGCGGGAGAAGGAGCTGAACATATACAGGAGTGGGTCAGCGCCGCCCACCGTGGAAGGCTCGTTGAATGCTGTCGGAGGCTTGTTTGAGGCGTCTGCACTGTCGGGTTTCACGAAAAACGGCACTAAAGGGTTTGCGACCGAAGAGGAGCTTCTCGCTGATCCAGCTTATGTGAATTACTACTATTCCAATGTTAATCTCAATCCACGGCTTCCGCCGCCGCTGCTTTCGAAGGAGGACTGGCGTTTTTCCCAGCGGTTGCAAGGCGGAGGTGGGGGTTCGGCAGTTGGAGATAGGAGGATAGGGGGTAGGAGTGGCGGTGAAGGGTCACTTTTTTCTGTGCAGCCGGGGATTGGAGGGAAGGAGGAGAATGGTGTGCAGGCGAGGAAGGGTGCGGCAGAGTGGGGCGGTGATGGGTTGATTGGGTTGCCAGGGTTGGGACTTGGGAGTAGGCAGAAGAGTATTGCAGAGATTATTCAGGATGATATACATAATACAAATGTCTCAAGGCACCCTTCTCGTCCTGCTAGCCGGAATGCGTTTGATGATGGTGTTGAAGCTTCAGATACCCAGTTTGCTCATTTACAGGGAATGTCAGCTGTTCAGAACGGCGGTTCATCTAGTTCTCATACATATGCTTCTGCTCTGGGTGCTTCATTGTCGAGAAGCAACACACCAGACCCTCAGCTTGTAGCTAGGGCTCCTAGTCCTCGAATTCCACCAGTTGGAAGGGTTTCCTCCATGGATAAAAAAATTGGCAATGGTCAAAACTCATTCAATGGTGCCTCACTTAATGTGAATGAGTCTGCAGATCTGGCAGCTGCTTTATCTGGTATGAACCTGTCAACAAATGGTAGGATTGATGAGGACAATCGTGCACGCTCACAAATGCAACATGAAATTGATAATCACCATAACCTTTATGACATGCAAGGTGATTGGAACCATATTAAACAAAATTCGTACTTGAATAAGTCTGATTCTGGGAATTTTCATCTGCATTCTGCTTCCCAATCTTCTAACAAATCTTATCAGAATATGGGAAGAGGCAGTGGATTTGGTAGGGATCTGAACAGCCCTTCTTATATGTCTGACAATCTAGTTGATATTAATAATCCTGCTGTATCTTCTGGTAACTCATACTTAAGAGGACCTGTGCCAGCTCTTAATGGCCGAGGAAGCTCACACTCCCACTATCAGAATGTAGAAAATACCTCATTTCCGAACTATGGCTATTCTGGTAGTCCTTCATCTCCATCAATGATGGGTAGTCCACTTGGTAATGGTAGTTTACCACCCTTATTTGAAAATGCTGCTGCTGCGTCTGCAATGGGTGGGTTGGACTCTGGAGCTTTTGGAGGGATGTCTCTAGGACCAAATCTGTTGGCTGCAGCTGCTGAATTGCAGAATATTAGGGTTGGAAATCATGGTGGTGGGAGTGCTCTTCAGGTTCCCATGATGGACCCATTGTATGTTCAGTACTTGAGATCAAATGAATATGCAGCTGCACAGCTTGCAGCCCTTAATGATCCCACAAAAGATAGGGAAGGCATGGGTAATACGTACATGGATTTACTTGGTTTGCAAAAAGCTTATTTGGGGCAGTTGCTTTCGCCCCAAAAATCACAATTTGGTGCTCCCTACATTGGCAAGTCTGGTAGCTTGAATCATGGTTATTATGGAAATCCAGCATATGGGCATGGGATGTCTTATTCTGGAACACCATTGGGTGGTCCCCTTCTTCCAAACTCTCCTGTTGGACCTGGTAGTCCTGCCAGGCATGGTGAGCGGAACTTGCGTTTTTCTTCTGGGTTGAGGAACATGGGTGGAAGTCTGATGGGAGCTTGGCACTCCGAAAATGGTGGAAATTTGGATGAAAGTTTTGCCTCCTCTTTACTAGATGAGTTCAAAAGCAATAAGACTAAATGTTTTGAACTTTCAGAGATTGCAGGGCATGTTGTTGAGTTCAGTGCCGACCAGTATGGAAGTCGGTTTATTCAACAAAAACTTGAAACTGCCACAATTGAAGAGAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCACCATCTCTTATGACCGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTTGAGCATGGAACTCCAGCACAAATAAGAGAACTTGCAGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATCCAGAAGGCTATAGAAGTTGTTGAGCTGGACCAGCAGACTAAAATGGTAGCTGAACTGGATGGTCATGTTATGCGTTGTGTTCGTGATCAAAATGGCAACCATGTTGTCCAGAAGTGTATTGAATGTGTACCTGAAGATGCCATCCAGTTTGTTGTTTCAACTTTTTATGACCAAGTCGTGACACTGTCTACTCATCCATATGGTTGTCGTGTCATACAGAGAGTTTTGGAGCACTGCCGTGACCCCAAAACGCAGCAAATAATGATGGATGAGATTTTGCAGTCTGTTTGCATTTTGGCACAAGACCAATATGGAAATTATGTTGTCCAGCATGTGCTGGAACATGGAAAGCCACATGAACGGTCATGTATAATTAAGGAACTAACTGGGCAAATCGTTCAAATGAGCCAGCAGAAGTTTGCGTCAAATGTCATAGAAAAGTGTTTAAGTTTTGGAACTCTTGCTGAACGCCAGGCCCTTGTAACTGAGATGCTTGGTACCACTGACGAAAATGAGCCCCTTCAGGCGATGATGAAGGATCAGTTCGCAAACTATGTTGTACAGAAAGTGTTGGAAACTTGTGACGATCAGCAACTTGAACTAATCCTTAACCGAATTAAGGTTCACCTGAATGCCCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCCCGTGTAGAGAAACTTGTTGCTGCTGGGGAGAGGAGGATTAGCATTCTGGCTCCCCAGGCTTCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1028

Amino Acids

111.27

Weight (kDa)

5.94

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 275 - 689 2.5e-152 Nucleic acid binding protein NABP
PUF PF00806 694 - 728 2.5e-11 Pumilio-family RNA binding repeat
PUF PF00806 733 - 763 2.1e-08 Pumilio-family RNA binding repeat
PUF PF00806 766 - 799 9.4e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 798 - 876 1e-05 NOP9-like PUF repeat domain
PUF PF00806 838 - 873 3.9e-13 Pumilio-family RNA binding repeat
PUF PF00806 876 - 909 8.7e-10 Pumilio-family RNA binding repeat
PUF PF00806 911 - 944 2.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 957 - 986 9.3e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1893
Acc65I GGTACC 1 cut(s) 2837
AccB1I GGYRCC 3 cut(s) 636, 908, 2837
AccB7I CCANNNNNTGG 3 cut(s) 851, 1820, 1856
AccBSI CCGCTC 2 cut(s) 130, 1915
AccI GTMKAC 2 cut(s) 2528, 2987
AclWI GGATC 5 cut(s) 281, 1200, 1649, 2332, 2886
AcoI YGGCCR 1 cut(s) 1297
AcsI RAATTY 4 cut(s) 840, 1084, 1114, 1993
AcuI CTGAAG 5 cut(s) 672, 1556, 2493, 2846, 2999
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 7 cut(s) 88, 1091, 1607, 1691, 2469, 2839, 2901
AfiI CCNNNNNNNGG 8 cut(s) 479, 563, 851, 1355, 1772, 1820, 1856, 2959
AflII CTTAAG 1 cut(s) 1270
AjnI CCWGG 5 cut(s) 556, 1888, 1901, 2811, 3066
AleI CACNNNNGTG 1 cut(s) 2577
Alw21I GWGCWC 4 cut(s) 1006, 1570, 1765, 2574
Alw26I GTCTC 2 cut(s) 634, 1497
Alw44I GTGCAC 1 cut(s) 1002
AlwI GGATC 5 cut(s) 281, 1200, 1649, 2332, 2886
AlwNI CAGNNNCTG 1 cut(s) 1523
AoxI GGCC 2 cut(s) 1297, 2814
ApaLI GTGCAC 1 cut(s) 1002
ApoI RAATTY 4 cut(s) 840, 1084, 1114, 1993
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AseI ATTAAT 1 cut(s) 1236
Asp700I GAANNNNTTC 1 cut(s) 859
Asp718I GGTACC 1 cut(s) 2837
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 7 cut(s) 1277, 1499, 1588, 1859, 1886, 2368, 2815
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 6 cut(s) 458, 548, 1028, 1073, 1922, 2957
AsuII TTCGAA 1 cut(s) 356
AsuNHI GCTAGC 1 cut(s) 653
AvaII GGWCC 6 cut(s) 1277, 1499, 1588, 1859, 1886, 2368
BaeGI GKGCMC 1 cut(s) 1006
BanI GGYRCC 3 cut(s) 636, 908, 2837
BanII GRGCYC 2 cut(s) 829, 2859
Bbv12I GWGCWC 4 cut(s) 1006, 1570, 1765, 2574
BbvCI CCTCAGC 1 cut(s) 810
BceAI ACGGC 4 cut(s) 262, 350, 751, 2562
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 5 cut(s) 558, 1890, 1903, 2813, 3068
BclI TGATCA 1 cut(s) 2425
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 634, 1497
BfaI CTAG 7 cut(s) 654, 747, 822, 831, 1226, 1496, 2024
BfmI CTRYAG 3 cut(s) 933, 1515, 2349
BfoI RGCGCY 1 cut(s) 170
BfrI CTTAAG 1 cut(s) 1270
BglII AGATCT 1 cut(s) 937
BmcAI AGTACT 1 cut(s) 1607
Bme1390I CCNGG 6 cut(s) 474, 558, 1890, 1903, 2813, 3068
Bme18I GGWCC 6 cut(s) 1277, 1499, 1588, 1859, 1886, 2368
BmgT120I GGNCC 7 cut(s) 1277, 1499, 1588, 1859, 1886, 2368, 2815
BmiI GGNNCC 9 cut(s) 638, 828, 910, 1070, 1578, 1590, 1861, 2839, 3063
BmrFI CCNGG 6 cut(s) 474, 558, 1890, 1903, 2813, 3068
BmrI ACTGGG 3 cut(s) 691, 2301, 2736
BmsI GCATC 2 cut(s) 2467, 2820
BmtI GCTAGC 1 cut(s) 657
BmuI ACTGGG 3 cut(s) 691, 2301, 2736
BpmI CTGGAG 2 cut(s) 1494, 2241
Bpu10I CCTNAGC 2 cut(s) 810, 2309
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 616, 1630
BpuMI CCSGG 1 cut(s) 474
Bsa29I ATCGAT 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 1689
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 7 cut(s) 177, 332, 473, 557, 867, 1300, 3066
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 8 cut(s) 479, 563, 851, 1355, 1772, 1820, 1856, 2959
Bse1I ACTGG 8 cut(s) 371, 697, 848, 2104, 2296, 2400, 2485, 2731
Bse3DI GCAATG 2 cut(s) 892, 1461
BseBI CCWGG 5 cut(s) 558, 1890, 1903, 2813, 3068
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 7 cut(s) 177, 332, 473, 557, 867, 1300, 3066
BseGI GGATG 8 cut(s) 613, 1494, 1836, 2005, 2404, 2481, 2533, 2614
BseLI CCNNNNNNNGG 8 cut(s) 479, 563, 851, 1355, 1772, 1820, 1856, 2959
BseMI GCAATG 2 cut(s) 892, 1461
BseMII CTCAG 2 cut(s) 824, 2817
BseNI ACTGG 8 cut(s) 371, 697, 848, 2104, 2296, 2400, 2485, 2731
BseRI GAGGAG 4 cut(s) 287, 506, 2005, 3058
BseSI GKGCMC 1 cut(s) 1006
BseYI CCCAGC 2 cut(s) 378, 3035
BsgI GTGCAG 4 cut(s) 207, 488, 524, 1617
Bsh1285I CGRYCG 2 cut(s) 50, 267
BshFI GGCC 2 cut(s) 1299, 2816
BshNI GGYRCC 3 cut(s) 636, 908, 2837
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 2 cut(s) 50, 267
BsiHKAI GWGCWC 4 cut(s) 1006, 1570, 1765, 2574
BsiSI CCGG 2 cut(s) 473, 658
BslFI GGGAC 2 cut(s) 579, 1845
BslI CCNNNNNNNGG 8 cut(s) 479, 563, 851, 1355, 1772, 1820, 1856, 2959
BsmAI GTCTC 2 cut(s) 634, 1497
BsmFI GGGAC 2 cut(s) 579, 1845
BsmI GAATGC 5 cut(s) 199, 667, 1126, 2977, 3054
BsnI GGCC 2 cut(s) 1299, 2816
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 6 cut(s) 829, 1006, 1570, 1765, 2574, 2859
Bsp1407I TGTACA 1 cut(s) 2899
Bsp19I CCATGG 1 cut(s) 867
BspANI GGCC 2 cut(s) 1299, 2816
BspCNI CTCAG 2 cut(s) 823, 2818
BspDI ATCGAT 1 cut(s) 50
BspLI GGNNCC 9 cut(s) 638, 828, 910, 1070, 1578, 1590, 1861, 2839, 3063
BspMAI CTGCAG 2 cut(s) 937, 1519
BspOI GCTAGC 1 cut(s) 657
BspPI GGATC 5 cut(s) 281, 1200, 1649, 2332, 2886
BspQI GCTCTTC 1 cut(s) 1575
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 3 cut(s) 636, 908, 2837
BspTI CTTAAG 1 cut(s) 1270
BsrBI CCGCTC 2 cut(s) 130, 1915
BsrDI GCAATG 2 cut(s) 892, 1461
BsrGI TGTACA 1 cut(s) 2899
BsrI ACTGG 8 cut(s) 371, 697, 848, 2104, 2296, 2400, 2485, 2731
BssECI CCNNGG 7 cut(s) 177, 332, 473, 557, 867, 1300, 3066
BssNAI GTATAC 1 cut(s) 2988
BssNI GRCGYC 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 867
Bst1107I GTATAC 1 cut(s) 2988
Bst2UI CCWGG 5 cut(s) 558, 1890, 1903, 2813, 3068
Bst4CI ACNGT 4 cut(s) 178, 228, 2526, 2703
Bst6I CTCTTC 5 cut(s) 264, 578, 1165, 1575, 2148
BstACI GRCGYC 1 cut(s) 218
BstAFI CTTAAG 1 cut(s) 1270
BstAPI GCANNNNNTGC 1 cut(s) 1640
BstAUI TGTACA 1 cut(s) 2899
BstBAI YACGTR 1 cut(s) 1689
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 6 cut(s) 507, 655, 1006, 1287, 1641, 2750
BstDEI CTNAG 4 cut(s) 810, 2309, 2826, 3081
BstDSI CCRYGG 3 cut(s) 177, 332, 867
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 8 cut(s) 613, 1494, 1836, 2005, 2404, 2481, 2533, 2614
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstMAI GTCTC 2 cut(s) 634, 1497
BstMCI CGRYCG 2 cut(s) 50, 267
BstNI CCWGG 5 cut(s) 558, 1890, 1903, 2813, 3068
BstNSI RCATGY 4 cut(s) 1057, 2084, 2300, 2675
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 6 cut(s) 472, 556, 1888, 1901, 2811, 3066
BstSFI CTRYAG 3 cut(s) 933, 1515, 2349
BstSLI GKGCMC 1 cut(s) 1006
BstSNI TACGTA 1 cut(s) 1689
BstX2I RGATCY 2 cut(s) 937, 1192
BstXI CCANNNNNNTGG 1 cut(s) 2291
BstYI RGATCY 2 cut(s) 937, 1192
BstZ17I GTATAC 1 cut(s) 2988
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 2 cut(s) 1299, 2816
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 3 cut(s) 177, 332, 867
BtgZI GCGATG 1 cut(s) 2882
BtsCI GGATG 8 cut(s) 613, 1494, 1836, 2005, 2404, 2481, 2533, 2614
BtsI GCAGTG 2 cut(s) 1183, 2572
BtsIMutI CAGTG 7 cut(s) 224, 1183, 2101, 2289, 2522, 2572, 2841
Cac8I GCNNGC 6 cut(s) 507, 655, 1006, 1287, 1641, 2750
CaiI CAGNNNCTG 1 cut(s) 1523
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 7 cut(s) 1277, 1499, 1588, 1859, 1886, 2368, 2815
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 3 cut(s) 207, 1437, 2751
CsiI ACCWGGT 1 cut(s) 1888
Csp6I GTAC 7 cut(s) 87, 1090, 1606, 1690, 2468, 2838, 2900
CviQI GTAC 7 cut(s) 87, 1090, 1606, 1690, 2468, 2838, 2900
DdeI CTNAG 4 cut(s) 810, 2309, 2826, 3081
DrdI GACNNNNNNGTC 1 cut(s) 1893
DseDI GACNNNNNNGTC 1 cut(s) 1893
EaeI YGGCCR 1 cut(s) 1297
Eam1104I CTCTTC 5 cut(s) 264, 578, 1165, 1575, 2148
EarI CTCTTC 5 cut(s) 264, 578, 1165, 1575, 2148
EciI GGCGGA 2 cut(s) 330, 408
Eco105I TACGTA 1 cut(s) 1689
Eco130I CCWWGG 1 cut(s) 867
Eco24I GRGCYC 2 cut(s) 829, 2859
Eco47I GGWCC 6 cut(s) 1277, 1499, 1588, 1859, 1886, 2368
Eco57I CTGAAG 5 cut(s) 672, 1556, 2493, 2846, 2999
Eco91I GGTNACC 1 cut(s) 4
EcoO109I RGGNCCY 2 cut(s) 1277, 2815
EcoO65I GGTNACC 1 cut(s) 4
EcoRI GAATTC 1 cut(s) 840
EcoRII CCWGG 5 cut(s) 556, 1888, 1901, 2811, 3066
EcoT14I CCWWGG 1 cut(s) 867
EcoT38I GRGCYC 2 cut(s) 829, 2859
ErhI CCWWGG 1 cut(s) 867
FalI AAGNNNNNCTT 6 cut(s) 261, 293, 441, 473, 625, 657
FaqI GGGAC 2 cut(s) 579, 1845
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 3 cut(s) 760, 1819, 2539
FbaI TGATCA 1 cut(s) 2425
FblI GTMKAC 2 cut(s) 2528, 2987
FokI GGATG 8 cut(s) 620, 1501, 1843, 2012, 2411, 2468, 2520, 2621
FriOI GRGCYC 2 cut(s) 829, 2859
FspBI CTAG 7 cut(s) 654, 747, 822, 831, 1226, 1496, 2024
GlaI GCGC 1 cut(s) 168
GsaI CCCAGC 2 cut(s) 382, 3039
GsuI CTGGAG 2 cut(s) 1494, 2241
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 2 cut(s) 1299, 2816
HapII CCGG 2 cut(s) 473, 658
HgaI GACGC 3 cut(s) 207, 1437, 2751
HhaI GCGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 218
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HindIII AAGCTT 2 cut(s) 684, 1716
HinfI GANTC 6 cut(s) 94, 929, 1106, 1469, 1792, 2230
HpaII CCGG 2 cut(s) 473, 658
HphI GGTGA 6 cut(s) 458, 548, 1028, 1073, 1922, 2957
Hpy166II GTNNAC 9 cut(s) 101, 972, 1004, 1400, 1418, 2468, 2529, 2965, 2988
Hpy8I GTNNAC 9 cut(s) 101, 972, 1004, 1400, 1418, 2468, 2529, 2965, 2988
HpyCH4III ACNGT 4 cut(s) 178, 228, 2526, 2703
HpyCH4IV ACGT 2 cut(s) 89, 1688
HpyF3I CTNAG 4 cut(s) 810, 2309, 2826, 3081
HpySE526I ACGT 2 cut(s) 89, 1688
Hsp92I GRCGYC 1 cut(s) 218
HspAI GCGC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 2841
Ksp22I TGATCA 1 cut(s) 2425
LguI GCTCTTC 1 cut(s) 1575
LmnI GCTCC 8 cut(s) 139, 274, 832, 1475, 1768, 1967, 2569, 3067
LweI GCATC 2 cut(s) 2467, 2820
MabI ACCWGGT 1 cut(s) 1888
MaeI CTAG 7 cut(s) 654, 747, 822, 831, 1226, 1496, 2024
MaeII ACGT 2 cut(s) 89, 1688
MaeIII GTNAC 7 cut(s) 4, 453, 1259, 2518, 2579, 2821, 2921
MbiI CCGCTC 2 cut(s) 130, 1915
MfeI CAATTG 1 cut(s) 2148
MflI RGATCY 2 cut(s) 937, 1192
MhlI GDGCHC 6 cut(s) 829, 1006, 1570, 1765, 2574, 2859
MlyI GAGTC 2 cut(s) 938, 1463
MmeI TCCRAC 7 cut(s) 181, 394, 832, 1446, 1525, 1864, 2892
MroXI GAANNNNTTC 1 cut(s) 859
MslI CAYNNNNRTG 5 cut(s) 759, 1557, 2538, 2577, 2970
MspA1I CMGCKG 6 cut(s) 349, 382, 725, 947, 1520, 1631
MspCI CTTAAG 1 cut(s) 1270
MspI CCGG 2 cut(s) 473, 658
MspR9I CCNGG 6 cut(s) 474, 558, 1890, 1903, 2813, 3068
MunI CAATTG 1 cut(s) 2148
Mva1269I GAATGC 5 cut(s) 199, 667, 1126, 2977, 3054
MvaI CCWGG 5 cut(s) 558, 1890, 1903, 2813, 3068
NciI CCSGG 1 cut(s) 474
NcoI CCATGG 1 cut(s) 867
NdeI CATATG 3 cut(s) 760, 1819, 2539
NheI GCTAGC 1 cut(s) 653
NlaIV GGNNCC 9 cut(s) 638, 828, 910, 1070, 1578, 1590, 1861, 2839, 3063
NmeAIII GCCGAG 1 cut(s) 1325
NmuCI GTSAC 5 cut(s) 4, 453, 2518, 2579, 2921
NspI RCATGY 4 cut(s) 1057, 2084, 2300, 2675
NspV TTCGAA 1 cut(s) 356
OliI CACNNNNGTG 1 cut(s) 2577
PciSI GCTCTTC 1 cut(s) 1575
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PctI GAATGC 5 cut(s) 199, 667, 1126, 2977, 3054
PdmI GAANNNNTTC 1 cut(s) 859
PfeI GAWTC 4 cut(s) 94, 1106, 1792, 2230
PflFI GACNNNGTC 2 cut(s) 2513, 2524
PflMI CCANNNNNTGG 3 cut(s) 851, 1820, 1856
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 2 cut(s) 937, 1463
PpsI GAGTC 2 cut(s) 937, 1463
Ppu21I YACGTR 1 cut(s) 1689
PpuMI RGGWCCY 1 cut(s) 1277
PshBI ATTAAT 1 cut(s) 1236
Psp5II RGGWCCY 1 cut(s) 1277
Psp6I CCWGG 5 cut(s) 556, 1888, 1901, 2811, 3066
PspEI GGTNACC 1 cut(s) 4
PspFI CCCAGC 2 cut(s) 378, 3035
PspGI CCWGG 5 cut(s) 556, 1888, 1901, 2811, 3066
PspN4I GGNNCC 9 cut(s) 638, 828, 910, 1070, 1578, 1590, 1861, 2839, 3063
PspPI GGNCC 7 cut(s) 1277, 1499, 1588, 1859, 1886, 2368, 2815
PspPPI RGGWCCY 1 cut(s) 1277
PstI CTGCAG 2 cut(s) 937, 1519
PstNI CAGNNNCTG 1 cut(s) 1523
PsuI RGATCY 2 cut(s) 937, 1192
PsyI GACNNNGTC 2 cut(s) 2513, 2524
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 4 cut(s) 725, 947, 1520, 1631
RsaI GTAC 7 cut(s) 88, 1091, 1607, 1691, 2469, 2839, 2901
RsaNI GTAC 7 cut(s) 87, 1090, 1606, 1690, 2468, 2838, 2900
RseI CAYNNNNRTG 5 cut(s) 759, 1557, 2538, 2577, 2970
SapI GCTCTTC 1 cut(s) 1575
Sau96I GGNCC 7 cut(s) 1277, 1499, 1588, 1859, 1886, 2368, 2815
ScaI AGTACT 1 cut(s) 1607
SchI GAGTC 2 cut(s) 938, 1463
ScrFI CCNGG 6 cut(s) 474, 558, 1890, 1903, 2813, 3068
SduI GDGCHC 6 cut(s) 829, 1006, 1570, 1765, 2574, 2859
SexAI ACCWGGT 1 cut(s) 1888
SfaNI GCATC 2 cut(s) 2467, 2820
SfcI CTRYAG 3 cut(s) 933, 1515, 2349
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 6 cut(s) 1277, 1499, 1588, 1859, 1886, 2368
SmiMI CAYNNNNRTG 5 cut(s) 759, 1557, 2538, 2577, 2970
SmlI CTYRAG 3 cut(s) 631, 1270, 1609
SmoI CTYRAG 3 cut(s) 631, 1270, 1609
SnaBI TACGTA 1 cut(s) 1689
SspI AATATT 1 cut(s) 1537
SspMI CTAG 7 cut(s) 654, 747, 822, 831, 1226, 1496, 2024
StyD4I CCNGG 6 cut(s) 472, 556, 1888, 1901, 2811, 3066
StyI CCWWGG 1 cut(s) 867
TaaI ACNGT 4 cut(s) 178, 228, 2526, 2703
TaiI ACGT 2 cut(s) 92, 1691
TaqI TCGA 5 cut(s) 50, 356, 788, 838, 2332
TaqII GACCGA 2 cut(s) 281, 2222
TatI WGTACW 2 cut(s) 1605, 2899
TauI GCSGC 5 cut(s) 112, 172, 346, 349, 524
TfiI GAWTC 4 cut(s) 94, 1106, 1792, 2230
TscAI CASTG 7 cut(s) 231, 1183, 2101, 2296, 2529, 2579, 2848
TseFI GTSAC 5 cut(s) 4, 453, 2518, 2579, 2921
Tsp45I GTSAC 5 cut(s) 4, 453, 2518, 2579, 2921
TspRI CASTG 7 cut(s) 231, 1183, 2101, 2296, 2529, 2579, 2848
Tth111I GACNNNGTC 2 cut(s) 2513, 2524
Van91I CCANNNNNTGG 3 cut(s) 851, 1820, 1856
Vha464I CTTAAG 1 cut(s) 1270
VneI GTGCAC 1 cut(s) 1002
VpaK11BI GGWCC 6 cut(s) 1277, 1499, 1588, 1859, 1886, 2368
VspI ATTAAT 1 cut(s) 1236
XapI RAATTY 4 cut(s) 840, 1084, 1114, 1993
XceI RCATGY 4 cut(s) 1057, 2084, 2300, 2675
XmiI GTMKAC 2 cut(s) 2528, 2987
XmnI GAANNNNTTC 1 cut(s) 859
XspI CTAG 7 cut(s) 654, 747, 822, 831, 1226, 1496, 2024
ZrmI AGTACT 1 cut(s) 1607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.