Rw5G040710

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002776
Physical Location & Seq
Forward (+)
71957167 .. 71965263
8097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040710.1

Sequence Viewer

Length: 3270 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCTGGCGGTGGCGGTGGAAGTGGAGGTGGAGGGGGAGGTGGAGGAGGAGGAGGGGGTGTTGGCGGTGGTGGGGGTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGAGGCTTCCGCCCCCTTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCAGGGTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTGCGCCGTGACTTGATGACATCTGATGCTCTAAGATCTGGTGCAAATGGCCAGGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAGATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCAATAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCACCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCACTTGCCATCACAGATCAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCTAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATATGCCTTCTCCACAGTCGGCAAAAGGTTCATACTCAGATTTGGGTAAGAGCAATGGGGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGGCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGCTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGGGGTTTGGCTTCTGGACCAAACCTAGCAGCTGCTGCATCTGATTCACATAGTCTTGGTAGATTAGGGAGTCCGATTGCAGGGACCGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCACAGTATGGCGTTGGTGTTCCCTTGGGGGGTAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCTGTTATTCCCAACTCTCCAGTTGCCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATTCTGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTTGGAAATCGGAGGACATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGGCTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTGGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTTGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCATTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCCGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGCCACATGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAGCATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1089

Amino Acids

116.06

Weight (kDa)

5.83

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 325 - 750 3.4e-171 Nucleic acid binding protein NABP
PUF PF00806 756 - 790 2.9e-10 Pumilio-family RNA binding repeat
PUF PF00806 794 - 825 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 828 - 861 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 864 - 945 1.6e-07 NOP9-like PUF repeat domain
PUF PF00806 870 - 894 1e-05 Pumilio-family RNA binding repeat
PUF PF00806 900 - 935 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 940 - 970 8.4e-10 Pumilio-family RNA binding repeat
PUF PF00806 974 - 1007 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1019 - 1048 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2591
Acc65I GGTACC 1 cut(s) 1624
AccB1I GGYRCC 3 cut(s) 157, 1624, 1753
AccB7I CCANNNNNTGG 2 cut(s) 32, 886
AccII CGCG 1 cut(s) 3123
AciI CCGC 9 cut(s) 161, 188, 210, 216, 267, 410, 551, 614, 2285
AclI AACGTT 1 cut(s) 2687
AclWI GGATC 7 cut(s) 350, 528, 950, 1432, 1760, 1826, 2295
AcoI YGGCCR 3 cut(s) 859, 2463, 2587
AcsI RAATTY 7 cut(s) 62, 1120, 1492, 1873, 2125, 2131, 2276
AcuI CTGAAG 4 cut(s) 813, 1008, 2679, 3032
AcyI GRCGYC 2 cut(s) 158, 1754
AfaI GTAC 6 cut(s) 712, 1573, 1626, 1650, 2862, 3087
AfiI CCNNNNNNNGG 9 cut(s) 32, 653, 725, 886, 1004, 1371, 1736, 1955, 2662
AflIII ACRYGT 1 cut(s) 2266
AgeI ACCGGT 1 cut(s) 1742
AhlI ACTAGT 1 cut(s) 3004
AjuI GAANNNNNNNTTGG 4 cut(s) 1526, 1558, 2117, 2149
AloI GAACNNNNNNTCC 2 cut(s) 1346, 1378
Alw21I GWGCWC 3 cut(s) 1155, 2436, 2760
Alw26I GTCTC 5 cut(s) 1072, 1751, 2183, 2686, 3095
AlwI GGATC 7 cut(s) 350, 528, 950, 1432, 1760, 1826, 2295
AlwNI CAGNNNCTG 1 cut(s) 1691
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 6 cut(s) 23, 859, 889, 2463, 2587, 2664
ApaI GGGCCC 1 cut(s) 893
ApoI RAATTY 7 cut(s) 62, 1120, 1492, 1873, 2125, 2131, 2276
AsiGI ACCGGT 1 cut(s) 1742
Asp700I GAANNNNTTC 2 cut(s) 690, 1639
Asp718I GGTACC 1 cut(s) 1624
AspLEI GCGC 4 cut(s) 160, 816, 1756, 2601
AspS9I GGNCC 7 cut(s) 889, 890, 1673, 1740, 2153, 2554, 2984
AsuHPI GGTGA 4 cut(s) 1184, 1908, 2533, 3251
AsuNHI GCTAGC 1 cut(s) 1559
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 5 cut(s) 1673, 1740, 2153, 2554, 2984
BaeGI GKGCMC 1 cut(s) 893
BalI TGGCCA 2 cut(s) 861, 2465
BanI GGYRCC 3 cut(s) 157, 1624, 1753
BanII GRGCYC 2 cut(s) 893, 979
BauI CACGAG 1 cut(s) 3198
BbsI GAAGAC 1 cut(s) 1119
Bbv12I GWGCWC 3 cut(s) 1155, 2436, 2760
BbvCI CCTCAGC 1 cut(s) 347
BccI CCATC 3 cut(s) 623, 1171, 2452
BceAI ACGGC 4 cut(s) 735, 801, 2602, 2795
BclI TGATCA 2 cut(s) 1189, 2695
BcoDI GTCTC 5 cut(s) 1072, 1751, 2183, 2686, 3095
BcuI ACTAGT 1 cut(s) 3004
BfaI CTAG 9 cut(s) 522, 923, 972, 981, 1044, 1407, 1560, 1682, 3005
BfmI CTRYAG 4 cut(s) 873, 915, 1608, 1806
BfoI RGCGCY 2 cut(s) 161, 1757
BglII AGATCT 3 cut(s) 845, 1093, 1348
BlpI GCTNAGC 3 cut(s) 610, 1236, 2971
BmcAI AGTACT 1 cut(s) 1650
Bme18I GGWCC 5 cut(s) 1673, 1740, 2153, 2554, 2984
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 7 cut(s) 889, 890, 1673, 1740, 2153, 2554, 2984
BmtI GCTAGC 1 cut(s) 1563
BpiI GAAGAC 1 cut(s) 1119
BpmI CTGGAG 5 cut(s) 327, 1110, 1733, 2043, 2885
Bpu10I CCTNAGC 2 cut(s) 89, 347
Bpu1102I GCTNAGC 3 cut(s) 610, 1236, 2971
BpuEI CTTGAG 3 cut(s) 1807, 2358, 2774
BsaHI GRCGYC 2 cut(s) 158, 1754
BsaI GGTCTC 2 cut(s) 1751, 2183
BsaWI WCCGGW 1 cut(s) 1742
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 653, 725, 886, 1004, 1371, 1736, 1955, 2662
Bse118I RCCGGY 1 cut(s) 1742
Bse1I ACTGG 4 cut(s) 1579, 1969, 2060, 2868
Bse3DI GCAATG 3 cut(s) 1339, 2770, 3060
BseLI CCNNNNNNNGG 9 cut(s) 32, 653, 725, 886, 1004, 1371, 1736, 1955, 2662
BseMI GCAATG 3 cut(s) 1339, 2770, 3060
BseMII CTCAG 7 cut(s) 338, 624, 974, 1329, 1934, 2792, 3260
BseNI ACTGG 4 cut(s) 1579, 1969, 2060, 2868
BseRI GAGGAG 8 cut(s) 261, 264, 267, 353, 356, 1020, 1578, 3246
BseSI GKGCMC 1 cut(s) 893
BseYI CCCAGC 3 cut(s) 281, 2912, 3221
Bsh1236I CGCG 1 cut(s) 3123
BshFI GGCC 6 cut(s) 25, 861, 891, 2465, 2589, 2666
BshNI GGYRCC 3 cut(s) 157, 1624, 1753
BshTI ACCGGT 1 cut(s) 1742
BsiHKAI GWGCWC 3 cut(s) 1155, 2436, 2760
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 2 cut(s) 593, 1743
BsiWI CGTACG 1 cut(s) 710
BslFI GGGAC 6 cut(s) 503, 792, 1753, 2013, 2166, 2639
BslI CCNNNNNNNGG 9 cut(s) 32, 653, 725, 886, 1004, 1371, 1736, 1955, 2662
BsmAI GTCTC 5 cut(s) 1072, 1751, 2183, 2686, 3095
BsmFI GGGAC 6 cut(s) 503, 792, 1753, 2013, 2166, 2639
BsmI GAATGC 2 cut(s) 190, 3163
BsnI GGCC 6 cut(s) 25, 861, 891, 2465, 2589, 2666
Bso31I GGTCTC 2 cut(s) 1751, 2183
BsoBI CYCGRG 1 cut(s) 565
Bsp120I GGGCCC 1 cut(s) 889
Bsp1286I GDGCHC 5 cut(s) 893, 979, 1155, 2436, 2760
Bsp1407I TGTACA 1 cut(s) 3085
Bsp1720I GCTNAGC 3 cut(s) 610, 1236, 2971
Bsp19I CCATGG 3 cut(s) 1551, 2031, 2156
BspACI CCGC 9 cut(s) 161, 188, 210, 216, 267, 410, 551, 614, 2285
BspANI GGCC 6 cut(s) 25, 861, 891, 2465, 2589, 2666
BspCNI CTCAG 7 cut(s) 339, 623, 973, 1328, 1933, 2791, 3259
BspFNI CGCG 1 cut(s) 3123
BspMAI CTGCAG 4 cut(s) 877, 919, 1612, 1810
BspOI GCTAGC 1 cut(s) 1563
BspPI GGATC 7 cut(s) 350, 528, 950, 1432, 1760, 1826, 2295
BspT107I GGYRCC 3 cut(s) 157, 1624, 1753
BspTNI GGTCTC 2 cut(s) 1751, 2183
BsrDI GCAATG 3 cut(s) 1339, 2770, 3060
BsrFI RCCGGY 1 cut(s) 1742
BsrGI TGTACA 1 cut(s) 3085
BsrI ACTGG 4 cut(s) 1579, 1969, 2060, 2868
BssAI RCCGGY 1 cut(s) 1742
BssNI GRCGYC 2 cut(s) 158, 1754
BssSI CACGAG 1 cut(s) 3198
BssT1I CCWWGG 5 cut(s) 1542, 1551, 1950, 2031, 2156
Bst2BI CACGAG 1 cut(s) 3198
Bst4CI ACNGT 3 cut(s) 169, 1296, 1932
Bst6I CTCTTC 4 cut(s) 1006, 2208, 2655, 3051
BstACI GRCGYC 2 cut(s) 158, 1754
BstAPI GCANNNNNTGC 3 cut(s) 1159, 1691, 1817
BstAUI TGTACA 1 cut(s) 3085
BstC8I GCNNGC 7 cut(s) 208, 748, 1561, 1818, 2819, 2823, 3125
BstDSI CCRYGG 3 cut(s) 1551, 2031, 2156
BstENI CCTNNNNNAGG 1 cut(s) 1002
BstFNI CGCG 1 cut(s) 3123
BstH2I RGCGCY 2 cut(s) 161, 1757
BstHHI GCGC 4 cut(s) 160, 816, 1756, 2601
BstMAI GTCTC 5 cut(s) 1072, 1751, 2183, 2686, 3095
BstNSI RCATGY 5 cut(s) 871, 2014, 2270, 2821, 2861
BstSFI CTRYAG 4 cut(s) 873, 915, 1608, 1806
BstSLI GKGCMC 1 cut(s) 893
BstUI CGCG 1 cut(s) 3123
BstV2I GAAGAC 1 cut(s) 1119
BstX2I RGATCY 4 cut(s) 533, 845, 1093, 1348
BstYI RGATCY 4 cut(s) 533, 845, 1093, 1348
BsuRI GGCC 6 cut(s) 25, 861, 891, 2465, 2589, 2666
BtgI CCRYGG 3 cut(s) 1551, 2031, 2156
BtsI GCAGTG 1 cut(s) 2758
BtsIMutI CAGTG 2 cut(s) 2287, 2758
Cac8I GCNNGC 7 cut(s) 208, 748, 1561, 1818, 2819, 2823, 3125
CaiI CAGNNNCTG 1 cut(s) 1691
CfoI GCGC 4 cut(s) 160, 816, 1756, 2601
Cfr10I RCCGGY 1 cut(s) 1742
Cfr13I GGNCC 7 cut(s) 889, 890, 1673, 1740, 2153, 2554, 2984
CseI GACGC 2 cut(s) 89, 321
Csp6I GTAC 6 cut(s) 711, 1572, 1625, 1649, 2861, 3086
CspAI ACCGGT 1 cut(s) 1742
CspCI CAANNNNNGTGG 4 cut(s) 586, 621, 1321, 1356
CviQI GTAC 6 cut(s) 711, 1572, 1625, 1649, 2861, 3086
DinI GGCGCC 2 cut(s) 159, 1755
DrdI GACNNNNNNGTC 1 cut(s) 2591
DseDI GACNNNNNNGTC 1 cut(s) 2591
EaeI YGGCCR 3 cut(s) 859, 2463, 2587
Eam1104I CTCTTC 4 cut(s) 1006, 2208, 2655, 3051
EarI CTCTTC 4 cut(s) 1006, 2208, 2655, 3051
EciI GGCGGA 1 cut(s) 399
Eco130I CCWWGG 5 cut(s) 1542, 1551, 1950, 2031, 2156
Eco24I GRGCYC 2 cut(s) 893, 979
Eco31I GGTCTC 2 cut(s) 1751, 2183
Eco47I GGWCC 5 cut(s) 1673, 1740, 2153, 2554, 2984
Eco57I CTGAAG 4 cut(s) 813, 1008, 2679, 3032
Eco88I CYCGRG 1 cut(s) 565
EcoNI CCTNNNNNAGG 1 cut(s) 1002
EcoRI GAATTC 1 cut(s) 2276
EcoT14I CCWWGG 5 cut(s) 1542, 1551, 1950, 2031, 2156
EcoT22I ATGCAT 2 cut(s) 760, 3163
EcoT38I GRGCYC 2 cut(s) 893, 979
EgeI GGCGCC 2 cut(s) 159, 1755
EheI GGCGCC 2 cut(s) 159, 1755
ErhI CCWWGG 5 cut(s) 1542, 1551, 1950, 2031, 2156
FaqI GGGAC 6 cut(s) 503, 792, 1753, 2013, 2166, 2639
FauNDI CATATG 2 cut(s) 1281, 2725
FbaI TGATCA 2 cut(s) 1189, 2695
FriOI GRGCYC 2 cut(s) 893, 979
FspBI CTAG 9 cut(s) 522, 923, 972, 981, 1044, 1407, 1560, 1682, 3005
GlaI GCGC 4 cut(s) 159, 815, 1755, 2600
GsaI CCCAGC 3 cut(s) 285, 2916, 3225
GsuI CTGGAG 5 cut(s) 327, 1110, 1733, 2043, 2885
HaeII RGCGCY 2 cut(s) 161, 1757
HaeIII GGCC 6 cut(s) 25, 861, 891, 2465, 2589, 2666
HapII CCGG 2 cut(s) 593, 1743
HgaI GACGC 2 cut(s) 89, 321
HhaI GCGC 4 cut(s) 160, 816, 1756, 2601
Hin1I GRCGYC 2 cut(s) 158, 1754
Hin6I GCGC 4 cut(s) 158, 814, 1754, 2599
HinP1I GCGC 4 cut(s) 158, 814, 1754, 2599
HincII GTYRAC 1 cut(s) 2936
HindII GTYRAC 1 cut(s) 2936
HindIII AAGCTT 1 cut(s) 2471
HpaII CCGG 2 cut(s) 593, 1743
HphI GGTGA 4 cut(s) 1184, 1908, 2533, 3251
Hpy166II GTNNAC 3 cut(s) 505, 2356, 2936
Hpy8I GTNNAC 3 cut(s) 505, 2356, 2936
Hpy99I CGWCG 2 cut(s) 105, 337
HpyCH4III ACNGT 3 cut(s) 169, 1296, 1932
HpyCH4IV ACGT 2 cut(s) 2687, 3081
HpySE526I ACGT 2 cut(s) 2687, 3081
Hsp92I GRCGYC 2 cut(s) 158, 1754
HspAI GCGC 4 cut(s) 158, 814, 1754, 2599
KasI GGCGCC 2 cut(s) 157, 1753
KpnI GGTACC 1 cut(s) 1628
Ksp22I TGATCA 2 cut(s) 1189, 2695
MaeI CTAG 9 cut(s) 522, 923, 972, 981, 1044, 1407, 1560, 1682, 3005
MaeII ACGT 2 cut(s) 2687, 3081
MaeIII GTNAC 9 cut(s) 38, 128, 818, 1409, 1914, 1926, 2177, 2609, 2704
MflI RGATCY 4 cut(s) 533, 845, 1093, 1348
MhlI GDGCHC 5 cut(s) 893, 979, 1155, 2436, 2760
MlsI TGGCCA 2 cut(s) 861, 2465
MluNI TGGCCA 2 cut(s) 861, 2465
Mly113I GGCGCC 2 cut(s) 158, 1754
MlyI GAGTC 5 cut(s) 709, 1094, 1735, 2308, 2941
MmeI TCCRAC 2 cut(s) 315, 3078
Mox20I TGGCCA 2 cut(s) 861, 2465
Mph1103I ATGCAT 2 cut(s) 760, 3163
MroXI GAANNNNTTC 2 cut(s) 690, 1639
MscI TGGCCA 2 cut(s) 861, 2465
MseI TTAA 8 cut(s) 561, 1059, 1253, 1443, 1517, 2097, 2220, 3144
MslI CAYNNNNRTG 4 cut(s) 2724, 2728, 2763, 3156
Msp20I TGGCCA 2 cut(s) 861, 2465
MspA1I CMGCKG 2 cut(s) 614, 1688
MspI CCGG 2 cut(s) 593, 1743
Mva1269I GAATGC 2 cut(s) 190, 3163
MvnI CGCG 1 cut(s) 3123
NarI GGCGCC 2 cut(s) 158, 1754
NcoI CCATGG 3 cut(s) 1551, 2031, 2156
NdeI CATATG 2 cut(s) 1281, 2725
NheI GCTAGC 1 cut(s) 1559
NmuCI GTSAC 6 cut(s) 128, 818, 1914, 1926, 2609, 2704
NsiI ATGCAT 2 cut(s) 760, 3163
NspI RCATGY 5 cut(s) 871, 2014, 2270, 2821, 2861
PaeI GCATGC 1 cut(s) 2821
PaeR7I CTCGAG 1 cut(s) 565
PciI ACATGT 1 cut(s) 2266
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3163
PdmI GAANNNNTTC 2 cut(s) 690, 1639
Pfl23II CGTACG 1 cut(s) 710
PflMI CCANNNNNTGG 2 cut(s) 32, 886
PfoI TCCNGGA 3 cut(s) 304, 1630, 2020
PinAI ACCGGT 1 cut(s) 1742
PleI GAGTC 5 cut(s) 709, 1093, 1734, 2307, 2940
PluTI GGCGCC 2 cut(s) 161, 1757
PpsI GAGTC 5 cut(s) 709, 1093, 1734, 2307, 2940
PscI ACATGT 1 cut(s) 2266
Psp1406I AACGTT 1 cut(s) 2687
PspFI CCCAGC 3 cut(s) 281, 2912, 3221
PspLI CGTACG 1 cut(s) 710
PspOMI GGGCCC 1 cut(s) 889
PspPI GGNCC 7 cut(s) 889, 890, 1673, 1740, 2153, 2554, 2984
PspXI VCTCGAGB 1 cut(s) 565
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 877, 919, 1612, 1810
PstNI CAGNNNCTG 1 cut(s) 1691
PsuI RGATCY 4 cut(s) 533, 845, 1093, 1348
PvuII CAGCTG 1 cut(s) 1688
RsaI GTAC 6 cut(s) 712, 1573, 1626, 1650, 2862, 3087
RsaNI GTAC 6 cut(s) 711, 1572, 1625, 1649, 2861, 3086
RseI CAYNNNNRTG 4 cut(s) 2724, 2728, 2763, 3156
SaqAI TTAA 8 cut(s) 561, 1059, 1253, 1443, 1517, 2097, 2220, 3144
Sau96I GGNCC 7 cut(s) 889, 890, 1673, 1740, 2153, 2554, 2984
ScaI AGTACT 1 cut(s) 1650
SchI GAGTC 5 cut(s) 709, 1094, 1735, 2308, 2941
SduI GDGCHC 5 cut(s) 893, 979, 1155, 2436, 2760
SfcI CTRYAG 4 cut(s) 873, 915, 1608, 1806
SfoI GGCGCC 2 cut(s) 159, 1755
Sfr274I CTCGAG 1 cut(s) 565
SinI GGWCC 5 cut(s) 1673, 1740, 2153, 2554, 2984
SlaI CTCGAG 1 cut(s) 565
SmiMI CAYNNNNRTG 4 cut(s) 2724, 2728, 2763, 3156
SmlI CTYRAG 4 cut(s) 565, 1786, 2373, 2753
SmoI CTYRAG 4 cut(s) 565, 1786, 2373, 2753
SpeI ACTAGT 1 cut(s) 3004
SphI GCATGC 1 cut(s) 2821
SsiI CCGC 9 cut(s) 161, 188, 210, 216, 267, 410, 551, 614, 2285
SspDI GGCGCC 2 cut(s) 157, 1753
SspI AATATT 1 cut(s) 139
SspMI CTAG 9 cut(s) 522, 923, 972, 981, 1044, 1407, 1560, 1682, 3005
StyI CCWWGG 5 cut(s) 1542, 1551, 1950, 2031, 2156
TaaI ACNGT 3 cut(s) 169, 1296, 1932
TaiI ACGT 2 cut(s) 2690, 3084
TaqI TCGA 5 cut(s) 383, 425, 545, 566, 1436
TatI WGTACW 3 cut(s) 1648, 2860, 3085
TauI GCSGC 2 cut(s) 163, 553
Tru1I TTAA 8 cut(s) 561, 1059, 1253, 1443, 1517, 2097, 2220, 3144
Tru9I TTAA 8 cut(s) 561, 1059, 1253, 1443, 1517, 2097, 2220, 3144
TscAI CASTG 2 cut(s) 2287, 2765
TseFI GTSAC 6 cut(s) 128, 818, 1914, 1926, 2609, 2704
Tsp45I GTSAC 6 cut(s) 128, 818, 1914, 1926, 2609, 2704
TspGWI ACGGA 1 cut(s) 2351
TspRI CASTG 2 cut(s) 2287, 2765
Van91I CCANNNNNTGG 2 cut(s) 32, 886
VpaK11BI GGWCC 5 cut(s) 1673, 1740, 2153, 2554, 2984
XagI CCTNNNNNAGG 1 cut(s) 1002
XapI RAATTY 7 cut(s) 62, 1120, 1492, 1873, 2125, 2131, 2276
XceI RCATGY 5 cut(s) 871, 2014, 2270, 2821, 2861
XcmI CCANNNNNNNNNTGG 1 cut(s) 1571
XhoI CTCGAG 1 cut(s) 565
XmnI GAANNNNTTC 2 cut(s) 690, 1639
XspI CTAG 9 cut(s) 522, 923, 972, 981, 1044, 1407, 1560, 1682, 3005
ZrmI AGTACT 1 cut(s) 1650
Zsp2I ATGCAT 2 cut(s) 760, 3163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.