AT2G44220

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004413
Physical Location & Seq
Forward (+)
18283789 .. 18285837
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44220.1

Sequence Viewer

Length: 1212 bp
ATGATGAGAATGACCGGTTTCATAGTAGCTATGATGATAGCAGCGGCGATATTCACTACTTGCGTTGATGGAAACAAGTTCTTTTATCACCGGGAAATTAAAGTACTTCGACATTTGAAACGGTTCAACAAGCCTGCTCTTAAATCCATTAAGAGTGAAGATGGAGATGTAATAGATTGTGTTCCTATCACTAATCAACCAGCTTTTGATCATCATTTACTTAAAAACCATACCATCCAGATGAGACCGAGTTTCTACCCGGTAAGTGATTCTACGTACACCAAGAGGGAGGCAAAGGCTGTAACTCAGGTTTGGCACAAGGCTGGAGAGTGTCCCAAAAACACTGTTCCAATCAGAAGAACAAAGAAAGAAGATCTCTTGAGACCAAAATCCATCAGGAGTTTTGGGAGAAAGTCTCATCAAAGCATTCCAAGAACTACAACCTTTGATCCAACATTAGGTCATCAGTACGCGCTAATGGGTGTGAGAAATGGAAAATTTTATGGGACAGAAGTTGCAATAAATCTGTGGAAACCATATGTCCAAATTCCTAAAGAGTTTAGCTTGGCTCAGACTTGGGTTGTGTCCGGAAATGGCTCTAGTCTAAACACCATTGAAGCTGGTTGGCAGGTTTATCCAGAACTATATGATGATAATAATCCTAGATTCTTTGTTTATTGGACGCGTGATGGGTACCGAAAAACGGGATGCTACAACCTTCTCTGCTCAGGTTTTGTTCAAACGAGTAATCGATACACCGTAGGTGGATCAATCACCACCATGTCACGCTACCGTGGAACTCAATATGATCTCTCCGTACTTATATGGAAGGACCAAAAAACAGGAAACTGGTGGCTTAGAGTCAATGAAAAAGACGTTATTGGGTACTGGCCAGGCTCGTTGTTCAATTCTCTAGGAAGAGAAGCTACGAGAGTTGAATGGGGAGGTGAAATCATTAACTCAAAGACCGGTGGGAGACACACTACCACAGATATGGGAAGTGGGCATTTTGCAGATGAAGGTTTTAAGAAAGCAAGCTATTTCAGGAATCTTAAGATAGTTGATGGAACCAATACTCTTAGAGAACCACAAGGACTTTACTTCTTTGCTGATAAACATAACTGTTACAATGTTAAGACAGGAAATGGTGGAACTTCTTGGGGGGCTCATTTCTTCTATGGTGGTCCTGGTCGAAACGTTAAATGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

403

Amino Acids

45.97

Weight (kDa)

9.66

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 49 - 160 2e-42 Neprosin activation peptide
Neprosin PF03080 185 - 396 9.5e-87 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 619
Acc65I GGTACC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 693
AccII CGCG 2 cut(s) 473, 685
AccIII TCCGGA 1 cut(s) 587
AciI CCGC 1 cut(s) 44
AclI AACGTT 1 cut(s) 1197
AclWI GGATC 2 cut(s) 443, 775
AcoI YGGCCR 1 cut(s) 890
AcsI RAATTY 2 cut(s) 497, 546
AfaI GTAC 6 cut(s) 105, 278, 470, 695, 819, 887
AfiI CCNNNNNNNGG 2 cut(s) 458, 703
AflII CTTAAG 1 cut(s) 1052
AflIII ACRYGT 1 cut(s) 683
AgeI ACCGGT 2 cut(s) 14, 968
AgsI TTSAA 6 cut(s) 118, 127, 617, 740, 907, 938
AjnI CCWGG 2 cut(s) 892, 1186
AluBI AGCT 6 cut(s) 29, 203, 564, 620, 926, 1038
AluI AGCT 6 cut(s) 29, 203, 564, 620, 926, 1038
Alw26I GTCTC 4 cut(s) 238, 376, 420, 970
AlwI GGATC 2 cut(s) 443, 775
Aor13HI TCCGGA 1 cut(s) 587
AoxI GGCC 1 cut(s) 890
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 2 cut(s) 497, 546
AsiGI ACCGGT 2 cut(s) 14, 968
Asp700I GAANNNNTTC 1 cut(s) 122
Asp718I GGTACC 1 cut(s) 693
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 832, 1184
AsuC2I CCSGG 2 cut(s) 92, 260
AsuHPI GGTGA 3 cut(s) 80, 766, 959
AvaII GGWCC 2 cut(s) 832, 1184
BalI TGGCCA 1 cut(s) 892
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 1168
BarI GAAGNNNNNNTAC 2 cut(s) 910, 942
BbvI GCAGC 1 cut(s) 53
BccI CCATC 6 cut(s) 62, 155, 242, 401, 683, 1058
BciT130I CCWGG 2 cut(s) 894, 1188
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 2 cut(s) 92, 260
BcoDI GTCTC 4 cut(s) 238, 376, 420, 970
BfaI CTAG 3 cut(s) 600, 663, 914
BfrI CTTAAG 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 619
BglII AGATCT 1 cut(s) 373
BisI GCNGC 2 cut(s) 42, 45
BlsI GCNGC 2 cut(s) 43, 46
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 4 cut(s) 92, 260, 894, 1188
Bme18I GGWCC 2 cut(s) 832, 1184
BmgT120I GGNCC 2 cut(s) 832, 1184
BmiI GGNNCC 2 cut(s) 695, 1069
BmrFI CCNGG 4 cut(s) 92, 260, 894, 1188
BmsI GCATC 1 cut(s) 698
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 400
BpuMI CCSGG 2 cut(s) 92, 260
Bsa29I ATCGAT 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 657
BsaI GGTCTC 2 cut(s) 238, 376
BsaJI CCNNGG 1 cut(s) 793
BsaWI WCCGGW 3 cut(s) 14, 587, 968
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 703
Bse118I RCCGGY 2 cut(s) 14, 968
Bse1I ACTGG 2 cut(s) 854, 893
Bse8I GATNNNNATC 1 cut(s) 657
BseAI TCCGGA 1 cut(s) 587
BseBI CCWGG 2 cut(s) 894, 1188
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 1 cut(s) 793
BseGI GGATG 2 cut(s) 234, 713
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 2 cut(s) 458, 703
BseMII CTCAG 3 cut(s) 320, 584, 741
BseNI ACTGG 2 cut(s) 854, 893
BseXI GCAGC 1 cut(s) 53
Bsh1236I CGCG 2 cut(s) 473, 685
BshFI GGCC 1 cut(s) 892
BshNI GGYRCC 1 cut(s) 693
BshTI ACCGGT 2 cut(s) 14, 968
BshVI ATCGAT 1 cut(s) 751
BsiSI CCGG 5 cut(s) 15, 91, 260, 588, 969
BslFI GGGAC 2 cut(s) 318, 520
BslI CCNNNNNNNGG 2 cut(s) 458, 703
BsmAI GTCTC 4 cut(s) 238, 376, 420, 970
BsmFI GGGAC 2 cut(s) 318, 520
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 1 cut(s) 892
Bso31I GGTCTC 2 cut(s) 238, 376
Bsp1286I GDGCHC 1 cut(s) 1168
Bsp13I TCCGGA 1 cut(s) 587
Bsp143I GATC 5 cut(s) 208, 373, 448, 767, 808
BspACI CCGC 1 cut(s) 44
BspANI GGCC 1 cut(s) 892
BspCNI CTCAG 3 cut(s) 319, 583, 740
BspDI ATCGAT 1 cut(s) 751
BspEI TCCGGA 1 cut(s) 587
BspFNI CGCG 2 cut(s) 473, 685
BspLI GGNNCC 2 cut(s) 695, 1069
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 2 cut(s) 443, 775
BspT107I GGYRCC 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 1052
BspTNI GGTCTC 2 cut(s) 238, 376
BsrFI RCCGGY 2 cut(s) 14, 968
BsrI ACTGG 2 cut(s) 854, 893
BssAI RCCGGY 2 cut(s) 14, 968
BssECI CCNNGG 1 cut(s) 793
BssMI GATC 5 cut(s) 208, 373, 448, 767, 808
Bst2UI CCWGG 2 cut(s) 894, 1188
Bst4CI ACNGT 5 cut(s) 123, 346, 760, 794, 1124
Bst6I CTCTTC 1 cut(s) 913
BstAFI CTTAAG 1 cut(s) 1052
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 135, 1036
BstDEI CTNAG 5 cut(s) 306, 570, 727, 857, 1079
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 2 cut(s) 234, 713
BstFNI CGCG 2 cut(s) 473, 685
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 5 cut(s) 211, 376, 451, 770, 811
BstMAI GTCTC 4 cut(s) 238, 376, 420, 970
BstMBI GATC 5 cut(s) 208, 373, 448, 767, 808
BstNI CCWGG 2 cut(s) 894, 1188
BstSCI CCNGG 4 cut(s) 90, 258, 892, 1186
BstSNI TACGTA 1 cut(s) 276
BstUI CGCG 2 cut(s) 473, 685
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 373
BstXI CCANNNNNNTGG 1 cut(s) 994
BstYI RGATCY 1 cut(s) 373
Bsu15I ATCGAT 1 cut(s) 751
BsuRI GGCC 1 cut(s) 892
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 2 cut(s) 234, 713
BtsIMutI CAGTG 1 cut(s) 342
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 135, 1036
CfoI GCGC 1 cut(s) 475
Cfr10I RCCGGY 2 cut(s) 14, 968
Cfr13I GGNCC 2 cut(s) 832, 1184
ClaI ATCGAT 1 cut(s) 751
CseI GACGC 1 cut(s) 691
Csp6I GTAC 6 cut(s) 104, 277, 469, 694, 818, 886
CspAI ACCGGT 2 cut(s) 14, 968
CviAII CATG 1 cut(s) 781
CviQI GTAC 6 cut(s) 104, 277, 469, 694, 818, 886
DdeI CTNAG 5 cut(s) 306, 570, 727, 857, 1079
DpnI GATC 5 cut(s) 210, 375, 450, 769, 810
DpnII GATC 5 cut(s) 208, 373, 448, 767, 808
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 1 cut(s) 913
EarI CTCTTC 1 cut(s) 913
Eco105I TACGTA 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 1168
Eco31I GGTCTC 2 cut(s) 238, 376
Eco47I GGWCC 2 cut(s) 832, 1184
EcoRII CCWGG 2 cut(s) 892, 1186
EcoT38I GRGCYC 1 cut(s) 1168
FaeI CATG 1 cut(s) 784
FaqI GGGAC 2 cut(s) 318, 520
FatI CATG 1 cut(s) 780
FauNDI CATATG 1 cut(s) 538
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 2 cut(s) 42, 45
FokI GGATG 2 cut(s) 221, 720
FriOI GRGCYC 1 cut(s) 1168
Fsp4HI GCNGC 2 cut(s) 42, 45
FspBI CTAG 3 cut(s) 600, 663, 914
GlaI GCGC 1 cut(s) 474
GluI GCNGC 2 cut(s) 42, 45
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 1 cut(s) 892
HapII CCGG 5 cut(s) 15, 91, 260, 588, 969
HgaI GACGC 1 cut(s) 691
HhaI GCGC 1 cut(s) 475
Hin1II CATG 1 cut(s) 784
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 4 cut(s) 269, 666, 861, 1048
HpaII CCGG 5 cut(s) 15, 91, 260, 588, 969
HphI GGTGA 3 cut(s) 80, 766, 959
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 2 cut(s) 356, 573
Hpy188III TCNNGA 6 cut(s) 238, 379, 397, 588, 638, 1045
Hpy8I GTNNAC 1 cut(s) 279
HpyAV CCTTC 3 cut(s) 728, 823, 1013
HpyCH4III ACNGT 5 cut(s) 123, 346, 760, 794, 1124
HpyCH4IV ACGT 3 cut(s) 275, 876, 1197
HpyCH4V TGCA 2 cut(s) 518, 1013
HpyF3I CTNAG 5 cut(s) 306, 570, 727, 857, 1079
HpySE526I ACGT 3 cut(s) 275, 876, 1197
Hsp92II CATG 1 cut(s) 784
HspAI GCGC 1 cut(s) 473
Kpn2I TCCGGA 1 cut(s) 587
KpnI GGTACC 1 cut(s) 697
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 5 cut(s) 208, 373, 448, 767, 808
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 1 cut(s) 698
MaeI CTAG 3 cut(s) 600, 663, 914
MaeII ACGT 3 cut(s) 275, 876, 1197
MaeIII GTNAC 3 cut(s) 301, 783, 1124
MalI GATC 5 cut(s) 210, 375, 450, 769, 810
MboI GATC 5 cut(s) 208, 373, 448, 767, 808
MboII GAAGA 5 cut(s) 170, 369, 383, 930, 1165
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 1168
MlsI TGGCCA 1 cut(s) 892
MluCI AATT 4 cut(s) 96, 497, 546, 907
MluI ACGCGT 1 cut(s) 683
MluNI TGGCCA 1 cut(s) 892
MlyI GAGTC 1 cut(s) 870
MmeI TCCRAC 1 cut(s) 476
MnlI CCTC 3 cut(s) 279, 283, 938
Mox20I TGGCCA 1 cut(s) 892
MroI TCCGGA 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 122
MscI TGGCCA 1 cut(s) 892
MseI TTAA 9 cut(s) 99, 141, 150, 222, 957, 1026, 1053, 1134, 1200
MslI CAYNNNNRTG 3 cut(s) 239, 779, 992
Msp20I TGGCCA 1 cut(s) 892
MspA1I CMGCKG 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 1052
MspI CCGG 5 cut(s) 15, 91, 260, 588, 969
MspR9I CCNGG 4 cut(s) 92, 260, 894, 1188
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 2 cut(s) 894, 1188
MvnI CGCG 2 cut(s) 473, 685
NciI CCSGG 2 cut(s) 92, 260
NdeI CATATG 1 cut(s) 538
NdeII GATC 5 cut(s) 208, 373, 448, 767, 808
NlaIII CATG 1 cut(s) 784
NlaIV GGNNCC 2 cut(s) 695, 1069
NmuCI GTSAC 1 cut(s) 783
PctI GAATGC 1 cut(s) 426
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 269, 666, 1048
PinAI ACCGGT 2 cut(s) 14, 968
PkrI GCNGC 2 cut(s) 43, 46
PleI GAGTC 1 cut(s) 869
PpsI GAGTC 1 cut(s) 869
Ppu21I YACGTR 1 cut(s) 276
Psp1406I AACGTT 1 cut(s) 1197
Psp6I CCWGG 2 cut(s) 892, 1186
PspGI CCWGG 2 cut(s) 892, 1186
PspN4I GGNNCC 2 cut(s) 695, 1069
PspPI GGNCC 2 cut(s) 832, 1184
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 6 cut(s) 105, 278, 470, 695, 819, 887
RsaNI GTAC 6 cut(s) 104, 277, 469, 694, 818, 886
RseI CAYNNNNRTG 3 cut(s) 239, 779, 992
SaqAI TTAA 9 cut(s) 99, 141, 150, 222, 957, 1026, 1053, 1134, 1200
SatI GCNGC 2 cut(s) 42, 45
Sau3AI GATC 5 cut(s) 208, 373, 448, 767, 808
Sau96I GGNCC 2 cut(s) 832, 1184
ScaI AGTACT 1 cut(s) 105
SchI GAGTC 1 cut(s) 870
ScrFI CCNGG 4 cut(s) 92, 260, 894, 1188
SduI GDGCHC 1 cut(s) 1168
SfaNI GCATC 1 cut(s) 698
SinI GGWCC 2 cut(s) 832, 1184
SmiMI CAYNNNNRTG 3 cut(s) 239, 779, 992
SmlI CTYRAG 2 cut(s) 379, 1052
SmoI CTYRAG 2 cut(s) 379, 1052
SnaBI TACGTA 1 cut(s) 276
Sse9I AATT 4 cut(s) 96, 497, 546, 907
SsiI CCGC 1 cut(s) 44
SspMI CTAG 3 cut(s) 600, 663, 914
StyD4I CCNGG 4 cut(s) 90, 258, 892, 1186
TaaI ACNGT 5 cut(s) 123, 346, 760, 794, 1124
TaiI ACGT 3 cut(s) 278, 879, 1200
TaqI TCGA 3 cut(s) 109, 751, 1192
TaqII GACCGA 1 cut(s) 262
TasI AATT 4 cut(s) 96, 497, 546, 907
TatI WGTACW 1 cut(s) 103
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 269, 666, 1048
Tru1I TTAA 9 cut(s) 99, 141, 150, 222, 957, 1026, 1053, 1134, 1200
Tru9I TTAA 9 cut(s) 99, 141, 150, 222, 957, 1026, 1053, 1134, 1200
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 783
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 783
TspDTI ATGAA 3 cut(s) 10, 882, 1032
TspGWI ACGGA 1 cut(s) 805
TspRI CASTG 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 1052
VpaK11BI GGWCC 2 cut(s) 832, 1184
XapI RAATTY 2 cut(s) 497, 546
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 3 cut(s) 600, 663, 914
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.