Rorug02G0369200

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004413
Physical Location & Seq
Reverse (-)
47098328 .. 47102978
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0369200.1

Sequence Viewer

Length: 1008 bp
ATGAAAAACATTTATTCATGTTTGTTCCTTTTTGGCAGGTCTACTTCTCATGTCAACCACACTCCCTCTTGTTTGTCTCTCCTTCCGGCCCAGCCCATTCTACTCATCACAGCCACAGAAAACGACATCCAACGGTCCGTAGTCTTCTTCCACATCCCCTTCCATCAGCCTCAGGTTCATCGGAAACCCCCAATTCTCTTCCTCTCTCGCTCGCTCAGTGTGAGTGACTTCCGAGCTGAAACCAAAATGCTGGGCCGACTCGCCTCCACTCGGCTCCAAGAGATCCGCAGAATCTTCACTCAGCCTTCTTCGGTTCGATCCTTATCCACTGCTCTCAACTACCATATTGATTCGCCGGAGAACAATCCAGAACTTCCATGGGAATTTAGCGATGTTAACAAAGAGAAGGCGAAAGATATACTTTCTCATTATCCATCCAACTACAAGCAGTCTGCAGTGATTCCTCTGCTGGATCTTGCACAACAGCAGCATGGAGGATGGCTCCCTGTTTCAGCAATGAATGCAGTAGCAAAGGTTATAGAAGTTGCTCCTATACGTGTATATGAAGTTGCAACGTTTTATTCGATGTTCAACCGGACAAAGGTGGGCAAATATCATCTTTTGGTATGCGGCACAACACCATGTATGATTCGTGGCTCACGCGATATTGAATCAGCTTTACTGAAACACCTAGGAGTGAAACGCAATGAAGTAACCAAGGATGGATTATTCTCTGTCGGGGAAATGGAATGCATGGGATGCTGTGTGAATGCTCCTATGATTACAGTGGCAGATTACTCAAATGGATCGGAGGGATATACATACAACTATTATGAAGATGTTACTCCCGAGCGAGTTGTGGAAATAGTTGAGAAGTTAAGAAAAGGCGAGAAGCCTCCGGCCGGCACTCAAAATCCTAAGCGCATTAAGGGTGGACCAGAAGGAGGAAATACTACGTTGCTCGGTGAGCCAAAACCTCCTCCATGCCGGGATCTTGATGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

335

Amino Acids

37.43

Weight (kDa)

8.64

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2Fe-2S_thioredx PF01257 138 - 293 1e-50 Thioredoxin-like [2Fe-2S] ferredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 41
AccII CGCG 1 cut(s) 663
AciI CCGC 2 cut(s) 286, 630
AclI AACGTT 1 cut(s) 575
AclWI GGATC 5 cut(s) 277, 312, 480, 814, 999
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 1 cut(s) 383
AfiI CCNNNNNNNGG 4 cut(s) 270, 601, 902, 944
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 2 cut(s) 592, 671
AluBI AGCT 2 cut(s) 236, 677
AluI AGCT 2 cut(s) 236, 677
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 5 cut(s) 277, 312, 480, 814, 999
Ama87I CYCGRG 1 cut(s) 848
AoxI GGCC 3 cut(s) 87, 253, 900
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 1 cut(s) 383
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 1 cut(s) 924
AspS9I GGNCC 4 cut(s) 88, 135, 253, 935
AsuC2I CCSGG 1 cut(s) 989
AsuHPI GGTGA 1 cut(s) 977
AvaI CYCGRG 1 cut(s) 848
AvaII GGWCC 2 cut(s) 135, 935
AvrII CCTAGG 1 cut(s) 691
AxyI CCTNAGG 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 499
BccI CCATC 4 cut(s) 171, 442, 492, 716
BcnI CCSGG 1 cut(s) 989
BcoDI GTCTC 1 cut(s) 81
BfaI CTAG 2 cut(s) 692, 1006
BfmI CTRYAG 1 cut(s) 453
BfuAI ACCTGC 1 cut(s) 27
BisI GCNGC 2 cut(s) 488, 631
BlnI CCTAGG 1 cut(s) 691
BlsI GCNGC 2 cut(s) 489, 632
Bme1390I CCNGG 1 cut(s) 989
Bme18I GGWCC 2 cut(s) 135, 935
BmeT110I CYCGRG 1 cut(s) 848
BmgT120I GGNCC 4 cut(s) 88, 135, 253, 935
BmiI GGNNCC 2 cut(s) 275, 503
BmrFI CCNGG 1 cut(s) 989
BmsI GCATC 2 cut(s) 749, 988
BpiI GAAGAC 1 cut(s) 136
BplI GAGNNNNNCTC 2 cut(s) 486, 518
Bpu10I CCTNAGC 1 cut(s) 918
BpuMI CCSGG 1 cut(s) 989
BsaAI YACGTR 1 cut(s) 557
BsaBI GATNNNNATC 1 cut(s) 322
BsaJI CCNNGG 3 cut(s) 377, 691, 717
BsaWI WCCGGW 1 cut(s) 594
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 601, 902, 944
Bse118I RCCGGY 1 cut(s) 902
Bse21I CCTNAGG 1 cut(s) 171
Bse3DI GCAATG 2 cut(s) 522, 712
Bse8I GATNNNNATC 1 cut(s) 322
BseDI CCNNGG 3 cut(s) 377, 691, 717
BseGI GGATG 6 cut(s) 126, 153, 434, 503, 727, 764
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 4 cut(s) 270, 601, 902, 944
BseMI GCAATG 2 cut(s) 522, 712
BseMII CTCAG 3 cut(s) 185, 229, 314
BseRI GAGGAG 1 cut(s) 969
BseX3I CGGCCG 1 cut(s) 900
BseXI GCAGC 1 cut(s) 499
BseYI CCCAGC 2 cut(s) 90, 250
Bsh1236I CGCG 1 cut(s) 663
Bsh1285I CGRYCG 1 cut(s) 903
BshFI GGCC 3 cut(s) 89, 255, 902
BsiEI CGRYCG 1 cut(s) 903
BsiHKCI CYCGRG 1 cut(s) 848
BsiSI CCGG 6 cut(s) 86, 356, 595, 899, 903, 988
BslI CCNNNNNNNGG 4 cut(s) 270, 601, 902, 944
BsmAI GTCTC 1 cut(s) 81
BsmI GAATGC 3 cut(s) 526, 755, 775
BsnI GGCC 3 cut(s) 89, 255, 902
BsoBI CYCGRG 1 cut(s) 848
Bsp143I GATC 5 cut(s) 282, 317, 472, 806, 991
Bsp19I CCATGG 1 cut(s) 377
BspACI CCGC 2 cut(s) 286, 630
BspANI GGCC 3 cut(s) 89, 255, 902
BspCNI CTCAG 3 cut(s) 184, 228, 313
BspFNI CGCG 1 cut(s) 663
BspLI GGNNCC 2 cut(s) 275, 503
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 27
BspPI GGATC 5 cut(s) 277, 312, 480, 814, 999
BsrDI GCAATG 2 cut(s) 522, 712
BsrFI RCCGGY 1 cut(s) 902
BssAI RCCGGY 1 cut(s) 902
BssECI CCNNGG 3 cut(s) 377, 691, 717
BssMI GATC 5 cut(s) 282, 317, 472, 806, 991
BssT1I CCWWGG 3 cut(s) 377, 691, 717
Bst4CI ACNGT 2 cut(s) 135, 787
Bst6I CTCTTC 1 cut(s) 203
BstAPI GCANNNNNTGC 2 cut(s) 521, 759
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 3 cut(s) 212, 904, 1003
BstDEI CTNAG 4 cut(s) 171, 215, 300, 918
BstDSI CCRYGG 1 cut(s) 377
BstF5I GGATG 6 cut(s) 126, 153, 434, 503, 727, 764
BstFNI CGCG 1 cut(s) 663
BstHHI GCGC 1 cut(s) 924
BstKTI GATC 5 cut(s) 285, 320, 475, 809, 994
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 5 cut(s) 282, 317, 472, 806, 991
BstMCI CGRYCG 1 cut(s) 903
BstMWI GCNNNNNNNGC 3 cut(s) 521, 759, 967
BstNSI RCATGY 1 cut(s) 1005
BstSCI CCNGG 1 cut(s) 987
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 3 cut(s) 282, 472, 991
BstXI CCANNNNNNTGG 1 cut(s) 250
BstYI RGATCY 3 cut(s) 282, 472, 991
BstZI CGGCCG 1 cut(s) 900
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 3 cut(s) 89, 255, 902
BtgI CCRYGG 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 6 cut(s) 126, 153, 434, 503, 727, 764
BtsI GCAGTG 2 cut(s) 327, 462
BtsIMutI CAGTG 4 cut(s) 223, 327, 462, 792
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 3 cut(s) 212, 904, 1003
CfoI GCGC 1 cut(s) 924
Cfr10I RCCGGY 1 cut(s) 902
Cfr13I GGNCC 4 cut(s) 88, 135, 253, 935
CpoI CGGWCCG 1 cut(s) 135
CspI CGGWCCG 1 cut(s) 135
CviAII CATG 8 cut(s) 18, 50, 378, 491, 642, 754, 984, 1002
DdeI CTNAG 4 cut(s) 171, 215, 300, 918
DpnI GATC 5 cut(s) 284, 319, 474, 808, 993
DpnII GATC 5 cut(s) 282, 317, 472, 806, 991
EaeI YGGCCR 1 cut(s) 900
EagI CGGCCG 1 cut(s) 900
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
EclXI CGGCCG 1 cut(s) 900
Eco130I CCWWGG 3 cut(s) 377, 691, 717
Eco47I GGWCC 2 cut(s) 135, 935
Eco52I CGGCCG 1 cut(s) 900
Eco81I CCTNAGG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 848
EcoT14I CCWWGG 3 cut(s) 377, 691, 717
EcoT22I ATGCAT 2 cut(s) 755, 1003
ErhI CCWWGG 3 cut(s) 377, 691, 717
FaeI CATG 8 cut(s) 21, 53, 381, 494, 645, 757, 987, 1005
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 8 cut(s) 17, 49, 377, 490, 641, 753, 983, 1001
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 2 cut(s) 488, 631
FokI GGATG 6 cut(s) 113, 140, 421, 510, 734, 771
Fsp4HI GCNGC 2 cut(s) 488, 631
FspBI CTAG 2 cut(s) 692, 1006
GlaI GCGC 1 cut(s) 923
GluI GCNGC 2 cut(s) 488, 631
GsaI CCCAGC 2 cut(s) 94, 254
HaeIII GGCC 3 cut(s) 89, 255, 902
HapII CCGG 6 cut(s) 86, 356, 595, 899, 903, 988
HhaI GCGC 1 cut(s) 924
Hin1II CATG 8 cut(s) 21, 53, 381, 494, 645, 757, 987, 1005
Hin6I GCGC 1 cut(s) 922
HinP1I GCGC 1 cut(s) 922
HincII GTYRAC 2 cut(s) 55, 397
HindII GTYRAC 2 cut(s) 55, 397
HinfI GANTC 6 cut(s) 258, 291, 350, 460, 649, 671
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 6 cut(s) 86, 356, 595, 899, 903, 988
HphI GGTGA 1 cut(s) 977
Hpy166II GTNNAC 4 cut(s) 42, 55, 397, 935
Hpy188I TCNGA 3 cut(s) 183, 233, 811
Hpy188III TCNNGA 3 cut(s) 368, 848, 995
Hpy8I GTNNAC 4 cut(s) 42, 55, 397, 935
HpyAV CCTTC 5 cut(s) 92, 169, 315, 400, 935
HpyCH4III ACNGT 2 cut(s) 135, 787
HpyCH4IV ACGT 3 cut(s) 556, 575, 956
HpyCH4V TGCA 6 cut(s) 455, 479, 524, 572, 753, 1001
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 759, 967
HpyF3I CTNAG 4 cut(s) 171, 215, 300, 918
HpySE526I ACGT 3 cut(s) 556, 575, 956
Hsp92II CATG 8 cut(s) 21, 53, 381, 494, 645, 757, 987, 1005
HspAI GCGC 1 cut(s) 922
KroI GCCGGC 1 cut(s) 902
KroNI GCCGGC 1 cut(s) 904
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 5 cut(s) 282, 317, 472, 806, 991
LmnI GCTCC 4 cut(s) 279, 507, 553, 778
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 2 cut(s) 749, 988
MaeI CTAG 2 cut(s) 692, 1006
MaeII ACGT 3 cut(s) 556, 575, 956
MaeIII GTNAC 3 cut(s) 224, 712, 841
MalI GATC 5 cut(s) 284, 319, 474, 808, 993
MboI GATC 5 cut(s) 282, 317, 472, 806, 991
MboII GAAGA 6 cut(s) 136, 139, 190, 286, 300, 848
MflI RGATCY 3 cut(s) 282, 472, 991
MluCI AATT 2 cut(s) 192, 383
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 154, 462
Mph1103I ATGCAT 2 cut(s) 755, 1003
MroNI GCCGGC 1 cut(s) 902
MseI TTAA 3 cut(s) 396, 878, 927
MspI CCGG 6 cut(s) 86, 356, 595, 899, 903, 988
MspR9I CCNGG 1 cut(s) 989
Mva1269I GAATGC 3 cut(s) 526, 755, 775
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 3 cut(s) 521, 759, 967
NaeI GCCGGC 1 cut(s) 904
NciI CCSGG 1 cut(s) 989
NcoI CCATGG 1 cut(s) 377
NdeII GATC 5 cut(s) 282, 317, 472, 806, 991
NgoMIV GCCGGC 1 cut(s) 902
NlaIII CATG 8 cut(s) 21, 53, 381, 494, 645, 757, 987, 1005
NlaIV GGNNCC 2 cut(s) 275, 503
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 224
NsiI ATGCAT 2 cut(s) 755, 1003
NspI RCATGY 1 cut(s) 1005
PaeI GCATGC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 581
PctI GAATGC 3 cut(s) 526, 755, 775
PdiI GCCGGC 1 cut(s) 904
PfeI GAWTC 5 cut(s) 291, 350, 460, 649, 671
PkrI GCNGC 2 cut(s) 489, 632
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
Ppu21I YACGTR 1 cut(s) 557
Psp1406I AACGTT 1 cut(s) 575
PspFI CCCAGC 2 cut(s) 90, 250
PspN4I GGNNCC 2 cut(s) 275, 503
PspPI GGNCC 4 cut(s) 88, 135, 253, 935
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 3 cut(s) 282, 472, 991
Rsr2I CGGWCCG 1 cut(s) 135
RsrII CGGWCCG 1 cut(s) 135
SaqAI TTAA 3 cut(s) 396, 878, 927
SatI GCNGC 2 cut(s) 488, 631
Sau3AI GATC 5 cut(s) 282, 317, 472, 806, 991
Sau96I GGNCC 4 cut(s) 88, 135, 253, 935
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 989
SfaNI GCATC 2 cut(s) 749, 988
SfcI CTRYAG 1 cut(s) 453
SinI GGWCC 2 cut(s) 135, 935
SphI GCATGC 1 cut(s) 1005
Sse9I AATT 2 cut(s) 192, 383
SsiI CCGC 2 cut(s) 286, 630
SspMI CTAG 2 cut(s) 692, 1006
StyD4I CCNGG 1 cut(s) 987
StyI CCWWGG 3 cut(s) 377, 691, 717
TaaI ACNGT 2 cut(s) 135, 787
TaiI ACGT 3 cut(s) 559, 578, 959
TaqI TCGA 2 cut(s) 316, 584
TasI AATT 2 cut(s) 192, 383
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 5 cut(s) 291, 350, 460, 649, 671
Tru1I TTAA 3 cut(s) 396, 878, 927
Tru9I TTAA 3 cut(s) 396, 878, 927
TscAI CASTG 4 cut(s) 223, 334, 462, 792
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 7 cut(s) 6, 17, 167, 533, 579, 723, 849
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 4 cut(s) 223, 334, 462, 792
VpaK11BI GGWCC 2 cut(s) 135, 935
XapI RAATTY 1 cut(s) 383
XceI RCATGY 1 cut(s) 1005
XcmI CCANNNNNNNNNTGG 1 cut(s) 375
XmaJI CCTAGG 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 2 cut(s) 692, 1006
Zsp2I ATGCAT 2 cut(s) 755, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.