FvH4_6g32790

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004413
Physical Location & Seq
Reverse (-)
25881397 .. 25886892
5496 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32790.t1

Sequence Viewer

Length: 1824 bp
ATGGAGTATTATCGATCCCAAGGTTCCACCATAGTTAGGGCTTCCATTTTTATCATACTAGTAACAGTTTTCCTGCTGAGCAAGGGACTTGTTGATTGCAGAAAAGTTGAAGACAACAATTCGGTCATCAAAACTATAAAGGTCGATGGAGGTGAGGTGATAGATTGTGTTGACATCTATAAACAACCGGCTCTTAATCATCCACTTCTAAAAAATCATACAATACAGCTGAAACCAAGTGAAGAAATTGAAACGGAAAGTGTTCAAGATGATGACCTCTTACAGTCTTGGTTATTGAATGAAGAGTGTCCAGAGGGAACAATCCCCATTCTACTAACGCAGGCTTTCGAGAATATTTCCCTCCCTAAACCTAGCCCACATTTCAATGGCAGCAAACGCAGCTTTGCGCCTGGCGCACCTGGTCATGAGTATGCACAAGTTAGTTTGGTGAATGGGGGCTATTACGGAGCACACGCATATGTGAACGTATGGAGCCCTTTATCAGTGAATCAAGAGCTTACTTTAGCCCAGATTTGGGTTGTGGGAGGTGAAGGAAACGCAAGAAGTACTGTGGAAGCTGGGTGGACTATAAATTCTGGCAAGCCAAGGTTCTTCATATATTGGACTTTTGTGTTTTTGAAGGGCTATAACTACCTTGATGGTTGCTACAATCTTCAGTGTCCTGGTTTTATCCAACATCAATCAGCAGCTGGACAGTGGTGGCTTATGGTTCAAAACGAGGCATTAGGATACTGGCCGGAAAGCATATTACCAAGTCTACAAGACAGTGCTAATGTTGTTAGTTGGGGTGGTGAGGTTTACAATTCAGAAACTGGGGGTCGTCACACATCAACTCAAATGGGGAGTGGTCATTTTCCGAGTGAGGGCCTTGGCAAAGCGAGCTATTTTCGAAATCTCCAGTACTTGGCCAAGCCTGGAAAATTTGTTGATCCTCAAGGGCTTATAACATATGCAACAAAACCTTTATGCTACAATGGCGATGGAGGTGATGTGATAGATTGCATTGACATTTATAAGCAGCCTGCTCTTAGTCATCCACTTCTCAAAAATCATACTATTCAGCTGAATCCGCCAAGTTTGTTTCCAAATGGAACTAATGAAAGTTCTCATCAAGATGATGAAGTGCTGCAATCCTGGTTATTGAATGAAGAATGCCCGGAAGGAACAATCCCCATTCTTCGAACTCAGGCTTTTAATAGTTCTACTCCCAAATCTAGCCCACCGTTCAATGGCAGCGACGTTGAGTTCACACCTGCATCTGGTCATGAGGCCCATGGCTACAAAGATGGTTGCTACAATCTTCAATGTCCCGGTTTTGTCCAAGTAAACAGAAAGTTTGCCCTAGGTTTACCCATAAAACCTGTTTCAACCTACAATGGGAAGCAAGTTGAGATATTCATAAGTATATACAAGCACCAATCGACTGGACAGTGGTGGCGGCTTACGCTACAAGATCAGGCATTAGGATACTGGCCGGACAAAATCTTACCAAGTTTGAGAGGCAGTCTCGACACGATTAGCTGGGGTGGTGAGATTTTCAATTCAGAAGCTGAAGGTCATCACACATCATTGACATCAACTCAAATGGGGAGTGGTCATTTCCCAAGTGAAGGATATGGCAAAGCAAGCTATTTTCGAAATATCCAGTACTTGGCCAGCTCTGGAAAGTTTGTAGATGCACAAGGGCTTATCAACTATGTCACAAAGCCTCCATGCTACAACCTTATCATGAATAATAAAGCCCCAGGATTTGGAACCCATTTCTATTATGGGGGATCTGGATATTCAGCAAGATGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

608

Amino Acids

67.15

Weight (kDa)

5.77

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 45 - 114 7.3e-20 Neprosin activation peptide
Neprosin PF03080 171 - 235 2.1e-12 Neprosin
Neprosin PF03080 236 - 332 2.2e-29 Neprosin
Neprosin_AP PF14365 335 - 404 3.3e-21 Neprosin activation peptide
Neprosin PF03080 431 - 599 7.1e-52 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 965, 1035
AarI CACCTGC 1 cut(s) 1282
Acc36I ACCTGC 1 cut(s) 1282
AccB7I CCANNNNNTGG 3 cut(s) 925, 1672, 1772
AccI GTMKAC 1 cut(s) 778
AciI CCGC 2 cut(s) 1091, 1459
AclWI GGATC 3 cut(s) 9, 944, 1804
AcoI YGGCCR 4 cut(s) 755, 927, 1493, 1674
AcsI RAATTY 2 cut(s) 592, 941
AcuI CTGAAG 2 cut(s) 659, 1593
AfaI GTAC 3 cut(s) 568, 923, 1670
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 6 cut(s) 409, 418, 682, 934, 1154, 1765
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 1532
AlwI GGATC 3 cut(s) 9, 944, 1804
AlwNI CAGNNNCTG 3 cut(s) 710, 833, 1571
AoxI GGCC 6 cut(s) 755, 886, 927, 1290, 1493, 1674
ApeKI GCWGC 6 cut(s) 390, 399, 707, 1039, 1147, 1254
ApoI RAATTY 2 cut(s) 592, 941
Asp700I GAANNNNTTC 1 cut(s) 261
AspA2I CCTAGG 1 cut(s) 1363
AspLEI GCGC 2 cut(s) 409, 416
AspS9I GGNCC 2 cut(s) 886, 1291
AsuC2I CCSGG 2 cut(s) 1178, 1332
AsuHPI GGTGA 7 cut(s) 164, 169, 460, 560, 824, 1019, 1562
AsuII TTCGAA 3 cut(s) 910, 1201, 1657
AvrII CCTAGG 1 cut(s) 1363
BalI TGGCCA 2 cut(s) 929, 1676
BanII GRGCYC 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 6 cut(s) 402, 411, 719, 1051, 1134, 1266
BccI CCATC 4 cut(s) 140, 653, 995, 1301
BciT130I CCWGG 6 cut(s) 411, 420, 684, 936, 1156, 1767
BciVI GTATCC 2 cut(s) 743, 1481
BcnI CCSGG 2 cut(s) 1178, 1332
BcoDI GTCTC 1 cut(s) 1532
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 4 cut(s) 59, 372, 1236, 1364
BfuAI ACCTGC 1 cut(s) 1282
BfuI GTATCC 2 cut(s) 743, 1481
BisI GCNGC 7 cut(s) 391, 400, 708, 1040, 1148, 1255, 1460
BlnI CCTAGG 1 cut(s) 1363
BlpI GCTNAGC 1 cut(s) 77
BlsI GCNGC 7 cut(s) 392, 401, 709, 1041, 1149, 1256, 1461
BmcAI AGTACT 3 cut(s) 568, 923, 1670
Bme1390I CCNGG 8 cut(s) 411, 420, 684, 936, 1156, 1178, 1332, 1767
BmgT120I GGNCC 2 cut(s) 886, 1291
BmiI GGNNCC 3 cut(s) 25, 494, 1777
BmrFI CCNGG 8 cut(s) 411, 420, 684, 936, 1156, 1178, 1332, 1767
BmrI ACTGGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 1286, 1687, 1805
BmuI ACTGGG 1 cut(s) 843
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 1512, 1544
BpmI CTGGAG 1 cut(s) 902
Bpu1102I GCTNAGC 1 cut(s) 77
Bpu14I TTCGAA 3 cut(s) 910, 1201, 1657
BpuEI CTTGAG 1 cut(s) 939
BpuMI CCSGG 2 cut(s) 1178, 1332
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 6 cut(s) 19, 605, 889, 1294, 1363, 1765
BsaXI ACNNNNNCTCC 6 cut(s) 141, 171, 996, 1026, 1714, 1744
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 6 cut(s) 758, 838, 919, 1450, 1496, 1666
BseBI CCWGG 6 cut(s) 411, 420, 684, 936, 1156, 1767
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 6 cut(s) 19, 605, 889, 1294, 1363, 1765
BseGI GGATG 2 cut(s) 199, 1054
BseMII CTCAG 2 cut(s) 68, 1220
BseNI ACTGG 6 cut(s) 758, 838, 919, 1450, 1496, 1666
BseXI GCAGC 6 cut(s) 402, 411, 719, 1051, 1134, 1266
BseYI CCCAGC 2 cut(s) 578, 1542
BshFI GGCC 6 cut(s) 757, 888, 929, 1292, 1495, 1676
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 5 cut(s) 188, 758, 1178, 1332, 1496
BslFI GGGAC 2 cut(s) 99, 1314
BsmAI GTCTC 1 cut(s) 1532
BsmFI GGGAC 2 cut(s) 99, 1314
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 6 cut(s) 757, 888, 929, 1292, 1495, 1676
Bsp119I TTCGAA 3 cut(s) 910, 1201, 1657
Bsp1286I GDGCHC 2 cut(s) 472, 497
Bsp143I GATC 4 cut(s) 14, 949, 1474, 1796
Bsp1720I GCTNAGC 1 cut(s) 77
Bsp19I CCATGG 1 cut(s) 1294
BspACI CCGC 2 cut(s) 1091, 1459
BspANI GGCC 6 cut(s) 757, 888, 929, 1292, 1495, 1676
BspCNI CTCAG 2 cut(s) 69, 1219
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 3 cut(s) 424, 1285, 1749
BspLI GGNNCC 3 cut(s) 25, 494, 1777
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 3 cut(s) 9, 944, 1804
BspT104I TTCGAA 3 cut(s) 910, 1201, 1657
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 6 cut(s) 758, 838, 919, 1450, 1496, 1666
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 6 cut(s) 19, 605, 889, 1294, 1363, 1765
BssMI GATC 4 cut(s) 14, 949, 1474, 1796
BssT1I CCWWGG 5 cut(s) 19, 605, 889, 1294, 1363
Bst2UI CCWGG 6 cut(s) 411, 420, 684, 936, 1156, 1767
Bst4CI ACNGT 7 cut(s) 67, 285, 571, 717, 788, 1245, 1452
Bst6I CTCTTC 1 cut(s) 297
BstBI TTCGAA 3 cut(s) 910, 1201, 1657
BstC8I GCNNGC 6 cut(s) 342, 602, 901, 1044, 1648, 1678
BstDEI CTNAG 3 cut(s) 77, 1049, 1206
BstDSI CCRYGG 1 cut(s) 1294
BstF5I GGATG 2 cut(s) 199, 1054
BstHHI GCGC 2 cut(s) 409, 416
BstKTI GATC 4 cut(s) 17, 952, 1477, 1799
BstMAI GTCTC 1 cut(s) 1532
BstMBI GATC 4 cut(s) 14, 949, 1474, 1796
BstMWI GCNNNNNNNGC 8 cut(s) 396, 399, 413, 900, 996, 1090, 1465, 1647
BstNI CCWGG 6 cut(s) 411, 420, 684, 936, 1156, 1767
BstSCI CCNGG 8 cut(s) 409, 418, 682, 934, 1154, 1176, 1330, 1765
BstV1I GCAGC 6 cut(s) 402, 411, 719, 1051, 1134, 1266
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 1796
BstXI CCANNNNNNTGG 1 cut(s) 1445
BstYI RGATCY 1 cut(s) 1796
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 2 cut(s) 743, 1481
BsuRI GGCC 6 cut(s) 757, 888, 929, 1292, 1495, 1676
BsuTUI ATCGAT 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 1294
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 2 cut(s) 199, 1054
BtsIMutI CAGTG 5 cut(s) 510, 683, 722, 793, 1457
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 6 cut(s) 342, 602, 901, 1044, 1648, 1678
CaiI CAGNNNCTG 3 cut(s) 710, 833, 1571
CciI TCATGA 3 cut(s) 424, 1285, 1749
CfoI GCGC 2 cut(s) 409, 416
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 886, 1291
ClaI ATCGAT 1 cut(s) 13
CsiI ACCWGGT 1 cut(s) 418
Csp6I GTAC 3 cut(s) 567, 922, 1669
CviAII CATG 5 cut(s) 425, 1286, 1295, 1734, 1750
CviQI GTAC 3 cut(s) 567, 922, 1669
DdeI CTNAG 3 cut(s) 77, 1049, 1206
DpnI GATC 4 cut(s) 16, 951, 1476, 1798
DpnII GATC 4 cut(s) 14, 949, 1474, 1796
EaeI YGGCCR 4 cut(s) 755, 927, 1493, 1674
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
EciI GGCGGA 1 cut(s) 1080
Eco130I CCWWGG 5 cut(s) 19, 605, 889, 1294, 1363
Eco24I GRGCYC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 659, 1593
EcoO109I RGGNCCY 1 cut(s) 886
EcoRII CCWGG 6 cut(s) 409, 418, 682, 934, 1154, 1765
EcoT14I CCWWGG 5 cut(s) 19, 605, 889, 1294, 1363
EcoT38I GRGCYC 1 cut(s) 497
ErhI CCWWGG 5 cut(s) 19, 605, 889, 1294, 1363
FaeI CATG 5 cut(s) 428, 1289, 1298, 1737, 1753
FalI AAGNNNNNCTT 1 cut(s) 1804
FaqI GGGAC 2 cut(s) 99, 1314
FatI CATG 5 cut(s) 424, 1285, 1294, 1733, 1749
FauNDI CATATG 2 cut(s) 478, 970
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 7 cut(s) 391, 400, 708, 1040, 1148, 1255, 1460
FokI GGATG 2 cut(s) 186, 1041
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 7 cut(s) 391, 400, 708, 1040, 1148, 1255, 1460
FspBI CTAG 4 cut(s) 59, 372, 1236, 1364
GlaI GCGC 2 cut(s) 408, 415
GluI GCNGC 7 cut(s) 391, 400, 708, 1040, 1148, 1255, 1460
GsaI CCCAGC 2 cut(s) 582, 1546
GsuI CTGGAG 1 cut(s) 902
HaeIII GGCC 6 cut(s) 757, 888, 929, 1292, 1495, 1676
HapII CCGG 5 cut(s) 188, 758, 1178, 1332, 1496
HhaI GCGC 2 cut(s) 409, 416
Hin1II CATG 5 cut(s) 428, 1289, 1298, 1737, 1753
Hin6I GCGC 2 cut(s) 407, 414
HinP1I GCGC 2 cut(s) 407, 414
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 2 cut(s) 508, 1087
HpaII CCGG 5 cut(s) 188, 758, 1178, 1332, 1496
HphI GGTGA 7 cut(s) 164, 169, 460, 560, 824, 1019, 1562
Hpy166II GTNNAC 8 cut(s) 172, 484, 585, 779, 820, 1269, 1348, 1370
Hpy188I TCNGA 3 cut(s) 829, 879, 1567
Hpy8I GTNNAC 8 cut(s) 172, 484, 585, 779, 820, 1269, 1348, 1370
Hpy99I CGWCG 1 cut(s) 1262
HpyAV CCTTC 5 cut(s) 545, 634, 1175, 1568, 1625
HpyCH4III ACNGT 7 cut(s) 67, 285, 571, 717, 788, 1245, 1452
HpyCH4IV ACGT 2 cut(s) 486, 1260
HpyCH4V TGCA 7 cut(s) 99, 434, 974, 1023, 1150, 1277, 1700
HpyF10VI GCNNNNNNNGC 8 cut(s) 396, 399, 413, 900, 996, 1090, 1465, 1647
HpyF3I CTNAG 3 cut(s) 77, 1049, 1206
HpySE526I ACGT 2 cut(s) 486, 1260
Hsp92II CATG 5 cut(s) 428, 1289, 1298, 1737, 1753
HspAI GCGC 2 cut(s) 407, 414
Kzo9I GATC 4 cut(s) 14, 949, 1474, 1796
LmnI GCTCC 2 cut(s) 467, 492
Lsp1109I GCAGC 6 cut(s) 402, 411, 719, 1051, 1134, 1266
LweI GCATC 3 cut(s) 1286, 1687, 1805
MabI ACCWGGT 1 cut(s) 418
MaeI CTAG 4 cut(s) 59, 372, 1236, 1364
MaeII ACGT 2 cut(s) 486, 1260
MaeIII GTNAC 3 cut(s) 61, 842, 1720
MalI GATC 4 cut(s) 16, 951, 1476, 1798
MboI GATC 4 cut(s) 14, 949, 1474, 1796
MboII GAAGA 8 cut(s) 122, 254, 314, 604, 665, 1181, 1190, 1313
MflI RGATCY 1 cut(s) 1796
MhlI GDGCHC 2 cut(s) 472, 497
MlsI TGGCCA 2 cut(s) 929, 1676
MluCI AATT 6 cut(s) 118, 246, 592, 823, 941, 1561
MluNI TGGCCA 2 cut(s) 929, 1676
MmeI TCCRAC 1 cut(s) 718
Mox20I TGGCCA 2 cut(s) 929, 1676
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 929, 1676
MseI TTAA 3 cut(s) 195, 1215, 1822
MslI CAYNNNNRTG 4 cut(s) 384, 429, 477, 1134
Msp20I TGGCCA 2 cut(s) 929, 1676
MspA1I CMGCKG 3 cut(s) 229, 710, 1084
MspI CCGG 5 cut(s) 188, 758, 1178, 1332, 1496
MspR9I CCNGG 8 cut(s) 411, 420, 684, 936, 1156, 1178, 1332, 1767
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 6 cut(s) 411, 420, 684, 936, 1156, 1767
MwoI GCNNNNNNNGC 8 cut(s) 396, 399, 413, 900, 996, 1090, 1465, 1647
NciI CCSGG 2 cut(s) 1178, 1332
NcoI CCATGG 1 cut(s) 1294
NdeI CATATG 2 cut(s) 478, 970
NdeII GATC 4 cut(s) 14, 949, 1474, 1796
NlaIII CATG 5 cut(s) 428, 1289, 1298, 1737, 1753
NlaIV GGNNCC 3 cut(s) 25, 494, 1777
NmuCI GTSAC 2 cut(s) 842, 1720
NspV TTCGAA 3 cut(s) 910, 1201, 1657
PagI TCATGA 3 cut(s) 424, 1285, 1749
PaqCI CACCTGC 1 cut(s) 1282
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 508, 1087
PflMI CCANNNNNTGG 3 cut(s) 925, 1672, 1772
PkrI GCNGC 7 cut(s) 392, 401, 709, 1041, 1149, 1256, 1461
PsiI TTATAA 2 cut(s) 965, 1035
Psp6I CCWGG 6 cut(s) 409, 418, 682, 934, 1154, 1765
PspFI CCCAGC 2 cut(s) 578, 1542
PspGI CCWGG 6 cut(s) 409, 418, 682, 934, 1154, 1765
PspN4I GGNNCC 3 cut(s) 25, 494, 1777
PspPI GGNCC 2 cut(s) 886, 1291
PstNI CAGNNNCTG 3 cut(s) 710, 833, 1571
PsuI RGATCY 1 cut(s) 1796
PvuII CAGCTG 3 cut(s) 229, 710, 1084
RsaI GTAC 3 cut(s) 568, 923, 1670
RsaNI GTAC 3 cut(s) 567, 922, 1669
RseI CAYNNNNRTG 4 cut(s) 384, 429, 477, 1134
SaqAI TTAA 3 cut(s) 195, 1215, 1822
SatI GCNGC 7 cut(s) 391, 400, 708, 1040, 1148, 1255, 1460
Sau3AI GATC 4 cut(s) 14, 949, 1474, 1796
Sau96I GGNCC 2 cut(s) 886, 1291
ScaI AGTACT 3 cut(s) 568, 923, 1670
ScrFI CCNGG 8 cut(s) 411, 420, 684, 936, 1156, 1178, 1332, 1767
SduI GDGCHC 2 cut(s) 472, 497
SexAI ACCWGGT 1 cut(s) 418
SfaNI GCATC 3 cut(s) 1286, 1687, 1805
SfuI TTCGAA 3 cut(s) 910, 1201, 1657
SmiMI CAYNNNNRTG 4 cut(s) 384, 429, 477, 1134
SmlI CTYRAG 1 cut(s) 954
SmoI CTYRAG 1 cut(s) 954
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 6 cut(s) 118, 246, 592, 823, 941, 1561
SsiI CCGC 2 cut(s) 1091, 1459
SspI AATATT 1 cut(s) 355
SspMI CTAG 4 cut(s) 59, 372, 1236, 1364
StyD4I CCNGG 8 cut(s) 409, 418, 682, 934, 1154, 1176, 1330, 1765
StyI CCWWGG 5 cut(s) 19, 605, 889, 1294, 1363
TaaI ACNGT 7 cut(s) 67, 285, 571, 717, 788, 1245, 1452
TaiI ACGT 2 cut(s) 489, 1263
TaqI TCGA 8 cut(s) 13, 144, 348, 910, 1201, 1442, 1530, 1657
TaqII GACCGA 1 cut(s) 112
TasI AATT 6 cut(s) 118, 246, 592, 823, 941, 1561
TatI WGTACW 3 cut(s) 566, 921, 1668
TauI GCSGC 1 cut(s) 1462
TfiI GAWTC 2 cut(s) 508, 1087
Tru1I TTAA 3 cut(s) 195, 1215, 1822
Tru9I TTAA 3 cut(s) 195, 1215, 1822
TscAI CASTG 5 cut(s) 510, 683, 722, 793, 1457
TseFI GTSAC 2 cut(s) 842, 1720
TseI GCWGC 6 cut(s) 390, 399, 707, 1039, 1147, 1254
Tsp45I GTSAC 2 cut(s) 842, 1720
TspDTI ATGAA 7 cut(s) 315, 604, 1134, 1155, 1182, 1408, 1766
TspGWI ACGGA 2 cut(s) 269, 480
TspRI CASTG 5 cut(s) 510, 683, 722, 793, 1457
Van91I CCANNNNNTGG 3 cut(s) 925, 1672, 1772
XapI RAATTY 2 cut(s) 592, 941
XcmI CCANNNNNNNNNTGG 1 cut(s) 1787
XmaJI CCTAGG 1 cut(s) 1363
XmiI GTMKAC 1 cut(s) 778
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 4 cut(s) 59, 372, 1236, 1364
ZrmI AGTACT 3 cut(s) 568, 923, 1670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.