Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004413

Physical Location & Seq

Genomic Coordinates Forward (+)

18034561 .. 18039805

Gene Structure 5245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1198100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGGCAAATGGGATTAATTACACCAAAGTCGGAGGCTACTTTCTCATCGCAGCAGTGGCAAGTTTGATATTTGTTGATTGCAGAGAGATTGGAACAGTGCAAACTAAAGAGTCTCTGAAGCACAACAAAGCAGTCATCAAAACGATAAAGGGAGATGGCGGTCATGTGATAGATTGCGTTGATATCTATAAACAGCCAGCCCTTAATCACCCACTTCTCATAAATCACACCATAGAGCTGAAACCGAGTTCATATCCAAATGGAATTGAAGCCATAAGTCTTGAAGATGAGCTCTTTCAGTATTGGCGTCAGAATGGAGAGTGCCCCGAAGGAACAATCCCCATTGTACGAACACAGGGAGTTGATCATCATCGATCTAAACCACGGTTTAACCCTAAAGAAATTGGGTCCACTCCTTCAACTGGTCATGAGTATGCCCAGGTTGGTATGTACGATGGTGGATACTATGGAGCACAGGCAAGATTAAACGTATGGAACCCTGCATCGTTTAATGGAGAAATCAGTATAACCCAAACATGGATTGTGGGAGGCCAAGGACAAGAACTGAGCACTTTAGAAGCTGGCTGGCTTGTTAGATCGCCTGATAGCCAAACAAGGCTCTTCATATTTTGGACTGGTGATAATTACCAAGGTGGTTGCTACAATCTTGATTGTCCTGGTTTCGTCCAAATAAACAGCAAAGTTGCCATTGGCTATCCCATAACGCCTATTTCTAACTACAATGGGACTCAGTATGATATATTCTTAAGTATATACAAGAACACAGTGAGTGGACATTGGTGGCTTCAAGTTGGAAATGAGGCAGTTGGATATTGGCCGGACACCATCGTCCCAGGTTTAAGAGGCAGTGCTGAAATTATTAGTTGGGGTGGAGAGATTTTCAATTCAAAACCTGAGGGTCATCACACATCAACTCAAATGGGAAGTCATCATTTTCCTATCGAAGGATTTGGTAGGGCAAGCTATGTCCGCAATATCCAATATTTGGATAACTCTTCACAGTTCAAAGATGCTGAACATCTTAGAACATATGCCACAAATCCTAAATGCTACGATGTACTTGTTAAAGACAAAACCTCAAACTTTGGAACCCATATGTATTATGGAGGTCCTGGTTATTCAATACTATGTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

386

Amino Acids

42.79

Weight (kDa)

6.15

Isoelectric Point (pI)

26.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	48 - 134	4.7e-23	Neprosin activation peptide
Neprosin	PF03080	172 - 378	2.8e-70	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17750 AT2G44220 AT2G44240
fragaria_vesca	FvH4_6g32790
malus_domestica	MD09G1198100.v1.1
prunus_persica	Prupe.3G054100_v2.0.a1 Prupe.3G054200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0141911 RchiOBHm_Chr2g0141931 RchiOBHm_Chr2g0141971
rosa_laevigata	RLG00000019942
rosa_multiflora	Rmu_co8265735.1_g000001 Rmu_co8330971.1_g000001 Rmu_sc0001287.1_g000036 Rmu_sc0004757.1_g000021 Rmu_sc0009946.1_g000003 Rmu_sc0009946.1_g000009
rosa_roxburghii	Rroxscaffold_2G00103690 Rroxscaffold_2G00103720
rosa_rugosa	Rorug02G0369200 Rorug02G0369300 Rorug02G0369400
rosa_samantha	Rh2AG421400 Rh2AG421600 Rh2AG421700 Rh2AG422100 Rh2BG431900 Rh2CG408700 Rh2CG408900 Rh2DG441900
rosa_wichuraiana	Rw2G034570 Rw2G034580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	848
AccB7I	CCANNNNNTGG	1 cut(s)	1006
AciI	CCGC	2 cut(s)	159, 991
AcoI	YGGCCR	1 cut(s)	836
AcuI	CTGAAG	1 cut(s)	137
AcyI	GRCGYC	1 cut(s)	307
AfaI	GTAC	3 cut(s)	348, 452, 1080
AfiI	CCNNNNNNNGG	4 cut(s)	422, 537, 965, 1006
AflII	CTTAAG	1 cut(s)	766
AgsI	TTSAA	8 cut(s)	269, 284, 420, 809, 904, 909, 1027, 1143
AjnI	CCWGG	4 cut(s)	438, 676, 853, 1132
AluBI	AGCT	4 cut(s)	238, 292, 581, 984
AluI	AGCT	4 cut(s)	238, 292, 581, 984
Alw21I	GWGCWC	3 cut(s)	294, 475, 572
Alw26I	GTCTC	1 cut(s)	117
AoxI	GGCC	2 cut(s)	550, 836
ApeKI	GCWGC	1 cut(s)	50
ArsI	GACNNNNNNTTYG	2 cut(s)	931, 963
AseI	ATTAAT	1 cut(s)	15
AspS9I	GGNCC	2 cut(s)	408, 1130
AsuHPI	GGTGA	2 cut(s)	200, 650
AvaII	GGWCC	2 cut(s)	408, 1130
AxyI	CCTNAGG	1 cut(s)	915
BaeGI	GKGCMC	1 cut(s)	326
BanII	GRGCYC	1 cut(s)	294
Bbv12I	GWGCWC	3 cut(s)	294, 475, 572
BbvI	GCAGC	1 cut(s)	62
BccI	CCATC	3 cut(s)	149, 449, 854
BciT130I	CCWGG	4 cut(s)	440, 678, 855, 1134
BciVI	GTATCC	1 cut(s)	455
BclI	TGATCA	1 cut(s)	364
BcoDI	GTCTC	1 cut(s)	117
BfrI	CTTAAG	1 cut(s)	766
BfuI	GTATCC	1 cut(s)	455
BisI	GCNGC	1 cut(s)	51
BlsI	GCNGC	1 cut(s)	52
Bme1390I	CCNGG	4 cut(s)	440, 678, 855, 1134
Bme18I	GGWCC	2 cut(s)	408, 1130
BmgT120I	GGNCC	2 cut(s)	408, 1130
BmiI	GGNNCC	3 cut(s)	409, 497, 1111
BmrFI	CCNGG	4 cut(s)	440, 678, 855, 1134
BmsI	GCATC	2 cut(s)	512, 1021
Bsa29I	ATCGAT	1 cut(s)	373
BsaBI	GATNNNNATC	1 cut(s)	369
BsaHI	GRCGYC	1 cut(s)	307
BsaJI	CCNNGG	5 cut(s)	383, 438, 553, 649, 853
Bsc4I	CCNNNNNNNGG	4 cut(s)	422, 537, 965, 1006
Bse1I	ACTGG	2 cut(s)	427, 640
Bse21I	CCTNAGG	1 cut(s)	915
Bse8I	GATNNNNATC	1 cut(s)	369
BseBI	CCWGG	4 cut(s)	440, 678, 855, 1134
BseCI	ATCGAT	1 cut(s)	373
BseDI	CCNNGG	5 cut(s)	383, 438, 553, 649, 853
BseJI	GATNNNNATC	1 cut(s)	369
BseLI	CCNNNNNNNGG	4 cut(s)	422, 537, 965, 1006
BseMII	CTCAG	3 cut(s)	557, 764, 906
BseNI	ACTGG	2 cut(s)	427, 640
BseSI	GKGCMC	1 cut(s)	326
BseXI	GCAGC	1 cut(s)	62
BshFI	GGCC	2 cut(s)	552, 838
BshVI	ATCGAT	1 cut(s)	373
BsiHKAI	GWGCWC	3 cut(s)	294, 475, 572
BsiSI	CCGG	1 cut(s)	839
BslFI	GGGAC	3 cut(s)	760, 836, 1137
BslI	CCNNNNNNNGG	4 cut(s)	422, 537, 965, 1006
BsmAI	GTCTC	1 cut(s)	117
BsmFI	GGGAC	3 cut(s)	760, 836, 1137
BsnI	GGCC	2 cut(s)	552, 838
Bsp1286I	GDGCHC	4 cut(s)	294, 326, 475, 572
Bsp143I	GATC	3 cut(s)	364, 374, 596
BspACI	CCGC	2 cut(s)	159, 991
BspANI	GGCC	2 cut(s)	552, 838
BspCNI	CTCAG	3 cut(s)	558, 763, 907
BspDI	ATCGAT	1 cut(s)	373
BspHI	TCATGA	1 cut(s)	427
BspLI	GGNNCC	3 cut(s)	409, 497, 1111
BspQI	GCTCTTC	1 cut(s)	626
BspTI	CTTAAG	1 cut(s)	766
BsrI	ACTGG	2 cut(s)	427, 640
BssECI	CCNNGG	5 cut(s)	383, 438, 553, 649, 853
BssMI	GATC	3 cut(s)	364, 374, 596
BssNI	GRCGYC	1 cut(s)	307
BssT1I	CCWWGG	2 cut(s)	553, 649
Bst2UI	CCWGG	4 cut(s)	440, 678, 855, 1134
Bst4CI	ACNGT	4 cut(s)	97, 387, 787, 1023
Bst6I	CTCTTC	2 cut(s)	626, 1021
BstACI	GRCGYC	1 cut(s)	307
BstAFI	CTTAAG	1 cut(s)	766
BstC8I	GCNNGC	4 cut(s)	198, 583, 587, 982
BstDEI	CTNAG	4 cut(s)	566, 750, 915, 1043
BstDSI	CCRYGG	1 cut(s)	383
BstENI	CCTNNNNNAGG	1 cut(s)	963
BstKTI	GATC	3 cut(s)	367, 377, 599
BstMAI	GTCTC	1 cut(s)	117
BstMBI	GATC	3 cut(s)	364, 374, 596
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 990
BstNI	CCWGG	4 cut(s)	440, 678, 855, 1134
BstSCI	CCNGG	4 cut(s)	438, 676, 853, 1132
BstSLI	GKGCMC	1 cut(s)	326
BstV1I	GCAGC	1 cut(s)	62
Bsu15I	ATCGAT	1 cut(s)	373
Bsu36I	CCTNAGG	1 cut(s)	915
BsuI	GTATCC	1 cut(s)	455
BsuRI	GGCC	2 cut(s)	552, 838
BsuTUI	ATCGAT	1 cut(s)	373
BtgI	CCRYGG	1 cut(s)	383
BtgZI	GCGATG	1 cut(s)	31
BtsI	GCAGTG	2 cut(s)	60, 874
BtsIMutI	CAGTG	4 cut(s)	60, 102, 792, 874
Cac8I	GCNNGC	4 cut(s)	198, 583, 587, 982
CciI	TCATGA	1 cut(s)	427
Cfr13I	GGNCC	2 cut(s)	408, 1130
ClaI	ATCGAT	1 cut(s)	373
CseI	GACGC	1 cut(s)	296
Csp6I	GTAC	3 cut(s)	347, 451, 1079
CviAII	CATG	3 cut(s)	164, 428, 537
CviQI	GTAC	3 cut(s)	347, 451, 1079
DdeI	CTNAG	4 cut(s)	566, 750, 915, 1043
DpnI	GATC	3 cut(s)	366, 376, 598
DpnII	GATC	3 cut(s)	364, 374, 596
DrdI	GACNNNNNNGTC	1 cut(s)	848
DseDI	GACNNNNNNGTC	1 cut(s)	848
EaeI	YGGCCR	1 cut(s)	836
Eam1104I	CTCTTC	2 cut(s)	626, 1021
EarI	CTCTTC	2 cut(s)	626, 1021
Ecl136II	GAGCTC	1 cut(s)	292
Eco130I	CCWWGG	2 cut(s)	553, 649
Eco24I	GRGCYC	1 cut(s)	294
Eco32I	GATATC	1 cut(s)	184
Eco47I	GGWCC	2 cut(s)	408, 1130
Eco53kI	GAGCTC	1 cut(s)	292
Eco57I	CTGAAG	1 cut(s)	137
Eco81I	CCTNAGG	1 cut(s)	915
EcoICRI	GAGCTC	1 cut(s)	292
EcoNI	CCTNNNNNAGG	1 cut(s)	963
EcoO109I	RGGNCCY	1 cut(s)	1130
EcoRII	CCWGG	4 cut(s)	438, 676, 853, 1132
EcoRV	GATATC	1 cut(s)	184
EcoT14I	CCWWGG	2 cut(s)	553, 649
EcoT38I	GRGCYC	1 cut(s)	294
ErhI	CCWWGG	2 cut(s)	553, 649
FaeI	CATG	3 cut(s)	167, 431, 540
FaqI	GGGAC	3 cut(s)	760, 836, 1137
FatI	CATG	3 cut(s)	163, 427, 536
FauNDI	CATATG	2 cut(s)	1051, 1116
FbaI	TGATCA	1 cut(s)	364
Fnu4HI	GCNGC	1 cut(s)	51
FriOI	GRGCYC	1 cut(s)	294
Fsp4HI	GCNGC	1 cut(s)	51
GluI	GCNGC	1 cut(s)	51
HaeIII	GGCC	2 cut(s)	552, 838
HapII	CCGG	1 cut(s)	839
HgaI	GACGC	1 cut(s)	296
Hin1I	GRCGYC	1 cut(s)	307
Hin1II	CATG	3 cut(s)	167, 431, 540
HinfI	GANTC	2 cut(s)	110, 748
HpaII	CCGG	1 cut(s)	839
HphI	GGTGA	2 cut(s)	200, 650
Hpy166II	GTNNAC	2 cut(s)	411, 794
Hpy188I	TCNGA	3 cut(s)	32, 117, 312
Hpy188III	TCNNGA	3 cut(s)	281, 428, 668
Hpy8I	GTNNAC	2 cut(s)	411, 794
HpyAV	CCTTC	3 cut(s)	323, 426, 959
HpyCH4III	ACNGT	4 cut(s)	97, 387, 787, 1023
HpyCH4IV	ACGT	1 cut(s)	489
HpyCH4V	TGCA	3 cut(s)	81, 100, 503
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 990
HpyF3I	CTNAG	4 cut(s)	566, 750, 915, 1043
HpySE526I	ACGT	1 cut(s)	489
Hsp92I	GRCGYC	1 cut(s)	307
Hsp92II	CATG	3 cut(s)	167, 431, 540
Ksp22I	TGATCA	1 cut(s)	364
Kzo9I	GATC	3 cut(s)	364, 374, 596
LguI	GCTCTTC	1 cut(s)	626
LmnI	GCTCC	1 cut(s)	470
Lsp1109I	GCAGC	1 cut(s)	62
LweI	GCATC	2 cut(s)	512, 1021
MaeII	ACGT	1 cut(s)	489
MalI	GATC	3 cut(s)	366, 376, 598
MboI	GATC	3 cut(s)	364, 374, 596
MboII	GAAGA	3 cut(s)	296, 613, 1008
MhlI	GDGCHC	4 cut(s)	294, 326, 475, 572
MluCI	AATT	6 cut(s)	16, 264, 402, 643, 876, 904
MlyI	GAGTC	2 cut(s)	119, 742
MmeI	TCCRAC	3 cut(s)	10, 793, 808
MnlI	CCTC	7 cut(s)	26, 542, 814, 857, 910, 1108, 1121
MseI	TTAA	8 cut(s)	15, 204, 390, 485, 510, 767, 860, 1086
MslI	CAYNNNNRTG	1 cut(s)	432
MspCI	CTTAAG	1 cut(s)	766
MspI	CCGG	1 cut(s)	839
MspR9I	CCNGG	4 cut(s)	440, 678, 855, 1134
MvaI	CCWGG	4 cut(s)	440, 678, 855, 1134
MwoI	GCNNNNNNNGC	2 cut(s)	56, 990
NdeI	CATATG	2 cut(s)	1051, 1116
NdeII	GATC	3 cut(s)	364, 374, 596
NlaIII	CATG	3 cut(s)	167, 431, 540
NlaIV	GGNNCC	3 cut(s)	409, 497, 1111
PagI	TCATGA	1 cut(s)	427
PciSI	GCTCTTC	1 cut(s)	626
PflMI	CCANNNNNTGG	1 cut(s)	1006
PkrI	GCNGC	1 cut(s)	52
PleI	GAGTC	2 cut(s)	118, 742
PpsI	GAGTC	2 cut(s)	118, 742
PpuMI	RGGWCCY	1 cut(s)	1130
PshBI	ATTAAT	1 cut(s)	15
Psp124BI	GAGCTC	1 cut(s)	294
Psp5II	RGGWCCY	1 cut(s)	1130
Psp6I	CCWGG	4 cut(s)	438, 676, 853, 1132
PspGI	CCWGG	4 cut(s)	438, 676, 853, 1132
PspN4I	GGNNCC	3 cut(s)	409, 497, 1111
PspPI	GGNCC	2 cut(s)	408, 1130
PspPPI	RGGWCCY	1 cut(s)	1130
RsaI	GTAC	3 cut(s)	348, 452, 1080
RsaNI	GTAC	3 cut(s)	347, 451, 1079
RseI	CAYNNNNRTG	1 cut(s)	432
SacI	GAGCTC	1 cut(s)	294
SapI	GCTCTTC	1 cut(s)	626
SaqAI	TTAA	8 cut(s)	15, 204, 390, 485, 510, 767, 860, 1086
SatI	GCNGC	1 cut(s)	51
Sau3AI	GATC	3 cut(s)	364, 374, 596
Sau96I	GGNCC	2 cut(s)	408, 1130
SchI	GAGTC	2 cut(s)	119, 742
ScrFI	CCNGG	4 cut(s)	440, 678, 855, 1134
SduI	GDGCHC	4 cut(s)	294, 326, 475, 572
SfaNI	GCATC	2 cut(s)	512, 1021
SinI	GGWCC	2 cut(s)	408, 1130
SmiMI	CAYNNNNRTG	1 cut(s)	432
SmlI	CTYRAG	1 cut(s)	766
SmoI	CTYRAG	1 cut(s)	766
Sse9I	AATT	6 cut(s)	16, 264, 402, 643, 876, 904
SsiI	CCGC	2 cut(s)	159, 991
SspI	AATATT	1 cut(s)	1004
SstI	GAGCTC	1 cut(s)	294
StyD4I	CCNGG	4 cut(s)	438, 676, 853, 1132
StyI	CCWWGG	2 cut(s)	553, 649
TaaI	ACNGT	4 cut(s)	97, 387, 787, 1023
TaiI	ACGT	1 cut(s)	492
TaqI	TCGA	2 cut(s)	373, 963
TasI	AATT	6 cut(s)	16, 264, 402, 643, 876, 904
TatI	WGTACW	1 cut(s)	1078
Tru1I	TTAA	8 cut(s)	15, 204, 390, 485, 510, 767, 860, 1086
Tru9I	TTAA	8 cut(s)	15, 204, 390, 485, 510, 767, 860, 1086
TscAI	CASTG	4 cut(s)	60, 102, 792, 874
TseI	GCWGC	1 cut(s)	50
TspDTI	ATGAA	2 cut(s)	240, 613
TspRI	CASTG	4 cut(s)	60, 102, 792, 874
Van91I	CCANNNNNTGG	1 cut(s)	1006
Vha464I	CTTAAG	1 cut(s)	766
VpaK11BI	GGWCC	2 cut(s)	408, 1130
VspI	ATTAAT	1 cut(s)	15
XagI	CCTNNNNNAGG	1 cut(s)	963
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.