Rh2AG422100

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004413
Physical Location & Seq
Reverse (-)
62612645 .. 62614451
1807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG422100.1

Sequence Viewer

Length: 753 bp
ATGTTGGTCTTACTAGCTTTAGCTTGCTCATTGTTGGGTGCATTAGTTGATGGAACAAGACTTGTGACTGCCAATAATTTTGAATCAGTTAAGCACAAGGGAACAGTCAAAACCATAGAGAGTGACAGCGGTGATGTTATAGATTGTGTTGAGATTTACAAACAACCTGCTTTTGACCATCCCCTTCTCAGGAATCACACTATAGAGTTCAACTCCAACTCTCCAAGTGGAGTAAAACCAATAAACAACCAAGCCAAGCTTATTCAAGATTGGTTCAAGAATGGAGAATGCCCTGACGGAACGATCCCCATCATTAGGCCACAAAGATATGATTATAATTGGAGAGCCCCAAGGAAGCTTGATAACAACTTTGGTGATAATCCTTATGAAAATCATGAGTACGCCAAAGTTTTCTTGCCTGATGGAACCTACTATGGAGCACATGCAAGTATGAATGTGTGGACACCTGCAACTTACGATACTGACATTAGCCTAGCTCAGATTTGGGTTGTCTCTGGTGCAGGCGAACAACTGAACACTATCGAAGCAGGGTGGATCACTCATTCAGGTCGGAATCAAACAAGGATGTTCTTATACTGGACGAGGGACAATTACAATAGCACCGGTTGCTACAATCTTGACTGCCCTGGTTTCGTGCAAACCACCCGTAAATATGCAGTTGGTGCTGTTATACCACGTGTTTCCAGCTACAATGGGCCACAATGTTCGTTAGTTTTCACCATAGACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

250

Amino Acids

28.02

Weight (kDa)

5.84

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 38 - 112 4.3e-23 Neprosin activation peptide
Neprosin PF03080 161 - 248 2.3e-27 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 336
AarI CACCTGC 1 cut(s) 475
Acc36I ACCTGC 2 cut(s) 175, 475
AciI CCGC 1 cut(s) 129
AclWI GGATC 2 cut(s) 298, 563
AcvI CACGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 2 cut(s) 189, 315
AflIII ACRYGT 1 cut(s) 697
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 4 cut(s) 83, 211, 266, 277
AjnI CCWGG 1 cut(s) 646
AluBI AGCT 6 cut(s) 17, 23, 259, 358, 497, 708
AluI AGCT 6 cut(s) 17, 23, 259, 358, 497, 708
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 517
AlwI GGATC 2 cut(s) 298, 563
AoxI GGCC 2 cut(s) 317, 716
AsiGI ACCGGT 1 cut(s) 623
AspS9I GGNCC 1 cut(s) 716
AsuHPI GGTGA 3 cut(s) 143, 386, 730
BanII GRGCYC 1 cut(s) 349
BbrPI CACGTG 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 442
BccI CCATC 4 cut(s) 44, 186, 317, 416
BciT130I CCWGG 1 cut(s) 648
BcoDI GTCTC 1 cut(s) 517
BfaI CTAG 2 cut(s) 14, 494
BfmI CTRYAG 1 cut(s) 201
BfuAI ACCTGC 2 cut(s) 175, 475
Bme1390I CCNGG 1 cut(s) 648
BmgT120I GGNCC 1 cut(s) 716
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 648
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BsaAI YACGTR 1 cut(s) 698
BsaBI GATNNNNATC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 350, 646
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 315
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 1 cut(s) 602
Bse8I GATNNNNATC 1 cut(s) 308
BseBI CCWGG 1 cut(s) 648
BseDI CCNNGG 2 cut(s) 350, 646
BseGI GGATG 2 cut(s) 178, 591
BseJI GATNNNNATC 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 189, 315
BseMII CTCAG 2 cut(s) 202, 512
BseNI ACTGG 1 cut(s) 602
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 2 cut(s) 319, 718
BshTI ACCGGT 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 442
BsiSI CCGG 1 cut(s) 624
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 2 cut(s) 189, 315
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 1 cut(s) 620
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 319, 718
Bsp1286I GDGCHC 2 cut(s) 349, 442
Bsp143I GATC 2 cut(s) 303, 555
BspACI CCGC 1 cut(s) 129
BspANI GGCC 2 cut(s) 319, 718
BspCNI CTCAG 2 cut(s) 201, 511
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 427
BspMI ACCTGC 2 cut(s) 175, 475
BspPI GGATC 2 cut(s) 298, 563
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 1 cut(s) 602
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 350, 646
BssMI GATC 2 cut(s) 303, 555
BssT1I CCWWGG 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 648
Bst4CI ACNGT 1 cut(s) 106
BstAPI GCANNNNNTGC 2 cut(s) 627, 683
BstBAI YACGTR 1 cut(s) 698
BstC8I GCNNGC 2 cut(s) 25, 523
BstDEI CTNAG 2 cut(s) 188, 498
BstF5I GGATG 2 cut(s) 178, 591
BstKTI GATC 2 cut(s) 306, 558
BstMAI GTCTC 1 cut(s) 517
BstMBI GATC 2 cut(s) 303, 555
BstMWI GCNNNNNNNGC 2 cut(s) 627, 683
BstNI CCWGG 1 cut(s) 648
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 1 cut(s) 646
BstSFI CTRYAG 1 cut(s) 201
BsuRI GGCC 2 cut(s) 319, 718
BtsCI GGATG 2 cut(s) 178, 591
BveI ACCTGC 2 cut(s) 175, 475
Cac8I GCNNGC 2 cut(s) 25, 523
CciI TCATGA 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 1 cut(s) 716
Csp6I GTAC 1 cut(s) 400
CspAI ACCGGT 1 cut(s) 623
CviAII CATG 2 cut(s) 395, 443
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 188, 498
DpnI GATC 2 cut(s) 305, 557
DpnII GATC 2 cut(s) 303, 555
Eco130I CCWWGG 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 349
Eco72I CACGTG 1 cut(s) 698
EcoRII CCWGG 1 cut(s) 646
EcoT14I CCWWGG 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 2 cut(s) 398, 446
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 1 cut(s) 620
FatI CATG 2 cut(s) 394, 442
FokI GGATG 2 cut(s) 165, 598
FriOI GRGCYC 1 cut(s) 349
FspBI CTAG 2 cut(s) 14, 494
HaeIII GGCC 2 cut(s) 319, 718
HapII CCGG 1 cut(s) 624
Hin1II CATG 2 cut(s) 398, 446
HindIII AAGCTT 2 cut(s) 257, 356
HinfI GANTC 3 cut(s) 83, 193, 574
HpaII CCGG 1 cut(s) 624
HphI GGTGA 3 cut(s) 143, 386, 730
Hpy166II GTNNAC 1 cut(s) 462
Hpy188I TCNGA 2 cut(s) 501, 573
Hpy188III TCNNGA 5 cut(s) 190, 266, 277, 395, 638
Hpy8I GTNNAC 1 cut(s) 462
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 6 cut(s) 41, 446, 470, 521, 658, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 627, 683
HpyF3I CTNAG 2 cut(s) 188, 498
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 2 cut(s) 398, 446
Kzo9I GATC 2 cut(s) 303, 555
LmnI GCTCC 1 cut(s) 437
MaeI CTAG 2 cut(s) 14, 494
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 2 cut(s) 64, 122
MalI GATC 2 cut(s) 305, 557
MboI GATC 2 cut(s) 303, 555
MhlI GDGCHC 2 cut(s) 349, 442
MluCI AATT 3 cut(s) 76, 337, 610
MmeI TCCRAC 2 cut(s) 240, 551
MnlI CCTC 1 cut(s) 597
MseI TTAA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 1 cut(s) 624
MspR9I CCNGG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 1 cut(s) 648
MwoI GCNNNNNNNGC 2 cut(s) 627, 683
NdeII GATC 2 cut(s) 303, 555
NlaIII CATG 2 cut(s) 398, 446
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 64, 122
NspI RCATGY 1 cut(s) 446
PagI TCATGA 1 cut(s) 394
PaqCI CACCTGC 1 cut(s) 475
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 3 cut(s) 83, 193, 574
PinAI ACCGGT 1 cut(s) 623
PmaCI CACGTG 1 cut(s) 698
PmlI CACGTG 1 cut(s) 698
Ppu21I YACGTR 1 cut(s) 698
PsiI TTATAA 1 cut(s) 336
Psp6I CCWGG 1 cut(s) 646
PspCI CACGTG 1 cut(s) 698
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 1 cut(s) 716
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SaqAI TTAA 1 cut(s) 90
Sau3AI GATC 2 cut(s) 303, 555
Sau96I GGNCC 1 cut(s) 716
ScrFI CCNGG 1 cut(s) 648
SduI GDGCHC 2 cut(s) 349, 442
SfcI CTRYAG 1 cut(s) 201
Sse9I AATT 3 cut(s) 76, 337, 610
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 14, 494
StyD4I CCNGG 1 cut(s) 646
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 1 cut(s) 700
TaqI TCGA 1 cut(s) 543
TasI AATT 3 cut(s) 76, 337, 610
TfiI GAWTC 3 cut(s) 83, 193, 574
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TseFI GTSAC 2 cut(s) 64, 122
Tsp45I GTSAC 2 cut(s) 64, 122
TspDTI ATGAA 2 cut(s) 402, 467
TspGWI ACGGA 1 cut(s) 312
XceI RCATGY 1 cut(s) 446
XspI CTAG 2 cut(s) 14, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.