Rroxscaffold_2G00103720

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004413
Physical Location & Seq
Forward (+)
26703472 .. 26704882
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00103720.1

Sequence Viewer

Length: 414 bp
ATGATGGAGCCGAGGCAAGGGAGCATGAGAACGCTAGGAACTTCGATGGAGGAACACCAAGAAACGCCGACGGCTAGCGCTCGGATCTACACCGAGAATGCGATCCGGAAGCACACCAACACCAAATTCGAGAGGACGGCGGCGTCGGCCTTGTTAAGTACCGCCAAATGGGGGCTTAAAGTGCAAAATGAGGTATTAGGTTACTGGATTACTTGGGGTGGGGAGATTACTGATTCAAGAACAGGTGGTCGTCACACATCGACTCAAATGGAGAATGGTCATTTTCCTTATGAAGGCTACGGCAAAGCGAGCTATGTTGGCACTCTTCAGTACGCAGACAACGATAAAAACTTCAAAGATGTTCCTGTTGAAAGTCTTGAACATATGCCACAAAGCCTAGATGCTTCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

137

Amino Acids

15.35

Weight (kDa)

5.93

Isoelectric Point (pI)

22.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 70 - 127 1.5e-10 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 2 cut(s) 140, 162
AclWI GGATC 2 cut(s) 92, 97
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 2 cut(s) 160, 332
AfeI AGCGCT 1 cut(s) 79
AfiI CCNNNNNNNGG 4 cut(s) 17, 168, 171, 293
AgsI TTSAA 4 cut(s) 237, 355, 371, 380
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 1 cut(s) 312
AluI AGCT 1 cut(s) 312
AlwI GGATC 2 cut(s) 92, 97
Aor13HI TCCGGA 1 cut(s) 105
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 1 cut(s) 80
AsuNHI GCTAGC 1 cut(s) 74
BccI CCATC 1 cut(s) 40
BceAI ACGGC 3 cut(s) 87, 153, 316
BfaI CTAG 3 cut(s) 35, 75, 398
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 9
BmsI GCATC 1 cut(s) 391
BmtI GCTAGC 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 168, 171, 293
Bse1I ACTGG 1 cut(s) 209
BseAI TCCGGA 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 17, 168, 171, 293
BseNI ACTGG 1 cut(s) 209
BshFI GGCC 1 cut(s) 149
BsiSI CCGG 1 cut(s) 106
BslI CCNNNNNNNGG 4 cut(s) 17, 168, 171, 293
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 2 cut(s) 84, 102
BspACI CCGC 2 cut(s) 140, 162
BspANI GGCC 1 cut(s) 149
BspEI TCCGGA 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 9
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 92, 97
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 2 cut(s) 84, 102
BssNI GRCGYC 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 143
BstC8I GCNNGC 2 cut(s) 76, 310
BstENI CCTNNNNNAGG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 2 cut(s) 87, 105
BstMBI GATC 2 cut(s) 84, 102
BstMWI GCNNNNNNNGC 4 cut(s) 146, 181, 309, 318
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 149
Cac8I GCNNGC 2 cut(s) 76, 310
CfoI GCGC 1 cut(s) 80
CseI GACGC 1 cut(s) 132
Csp6I GTAC 2 cut(s) 159, 331
CviAII CATG 1 cut(s) 25
CviJI RGCY 7 cut(s) 10, 74, 149, 175, 297, 312, 396
CviKI_1 RGCY 7 cut(s) 10, 74, 149, 175, 297, 312, 396
CviQI GTAC 2 cut(s) 159, 331
DpnI GATC 2 cut(s) 86, 104
DpnII GATC 2 cut(s) 84, 102
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco47III AGCGCT 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 291
FaeI CATG 1 cut(s) 28
FaiI YATR 5 cut(s) 26, 291, 315, 384, 386
FatI CATG 1 cut(s) 24
FauNDI CATATG 1 cut(s) 384
Fnu4HI GCNGC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 3 cut(s) 35, 75, 398
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 106
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 1 cut(s) 28
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 2 cut(s) 233, 262
HpaII CCGG 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 4 cut(s) 106, 130, 237, 377
Hpy99I CGWCG 2 cut(s) 73, 148
HpyAV CCTTC 1 cut(s) 287
HpyCH4V TGCA 1 cut(s) 184
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 181, 309, 318
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 1 cut(s) 28
HspAI GCGC 1 cut(s) 78
Kpn2I TCCGGA 1 cut(s) 105
Kzo9I GATC 2 cut(s) 84, 102
LmnI GCTCC 2 cut(s) 7, 21
LpnPI CCDG 4 cut(s) 119, 190, 228, 378
LweI GCATC 1 cut(s) 391
MaeI CTAG 3 cut(s) 35, 75, 398
MaeIII GTNAC 2 cut(s) 200, 251
MalI GATC 2 cut(s) 86, 104
MboI GATC 2 cut(s) 84, 102
MboII GAAGA 1 cut(s) 317
MflI RGATCY 1 cut(s) 84
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 4 cut(s) 6, 43, 126, 184
MroI TCCGGA 1 cut(s) 105
MseI TTAA 2 cut(s) 155, 177
MspI CCGG 1 cut(s) 106
Mva1269I GAATGC 1 cut(s) 103
MwoI GCNNNNNNNGC 4 cut(s) 146, 181, 309, 318
NdeI CATATG 1 cut(s) 384
NdeII GATC 2 cut(s) 84, 102
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 1 cut(s) 9
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 9
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 2 cut(s) 160, 332
RsaNI GTAC 2 cut(s) 159, 331
SaqAI TTAA 2 cut(s) 155, 177
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 2 cut(s) 84, 102
SchI GAGTC 1 cut(s) 256
SetI ASST 4 cut(s) 195, 202, 247, 314
SfaNI GCATC 1 cut(s) 391
Sse9I AATT 1 cut(s) 125
SsiI CCGC 2 cut(s) 140, 162
SspMI CTAG 3 cut(s) 35, 75, 398
TaqI TCGA 3 cut(s) 44, 129, 260
TasI AATT 1 cut(s) 125
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 155, 177
Tru9I TTAA 2 cut(s) 155, 177
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 306
XagI CCTNNNNNAGG 1 cut(s) 291
XapI RAATTY 1 cut(s) 125
XspI CTAG 3 cut(s) 35, 75, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.