Rmu_sc0001287.1_g000036

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001287.1
OG0004413
Physical Location & Seq
Reverse (-)
185383 .. 185900
518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001287.1_g000036.1.cds

Sequence Viewer

Length: 387 bp
atgcagttcggcaatgactatgtgttggggtattggccttctttcctgttctcgtacttggccgacagtgcttccatgattgagtggggaggtgaggttgtaaattcgcagtcggatggccgacacacctcaactcaaatgggcagtggtcgttttccggaggagggttttggaaaatcaagttatttcaggaacattcaagttgttgatagctccaataatctcaaggcccccaaagggttaggcaccttcaccgagcagtcaaactgctatgatgttcagacaggaagtaatggggattggggtcactacttttactatggaggaccaggttgggctgtggctggtcttagtagtaaatcatgtgatggtcccttttctctctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

128

Amino Acids

13.98

Weight (kDa)

4.71

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccIII TCCGGA 1 cut(s) 157
AcoI YGGCCR 2 cut(s) 60, 118
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 164, 237
AgsI TTSAA 1 cut(s) 200
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 4 cut(s) 35, 60, 118, 228
ApoI RAATTY 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 229, 326, 371
AsuHPI GGTGA 2 cut(s) 104, 244
AvaII GGWCC 2 cut(s) 326, 371
BanI GGYRCC 1 cut(s) 245
BccI CCATC 2 cut(s) 110, 362
BciT130I CCWGG 1 cut(s) 330
BfaI CTAG 1 cut(s) 385
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 2 cut(s) 326, 371
BmgT120I GGNCC 3 cut(s) 229, 326, 371
BmiI GGNNCC 3 cut(s) 231, 247, 373
BmrFI CCNGG 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 237
Bse3DI GCAATG 1 cut(s) 19
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 1 cut(s) 330
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 164, 237
BseMI GCAATG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 4 cut(s) 37, 62, 120, 230
BshNI GGYRCC 1 cut(s) 245
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 164, 237
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 4 cut(s) 37, 62, 120, 230
Bsp13I TCCGGA 1 cut(s) 157
BspANI GGCC 4 cut(s) 37, 62, 120, 230
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 231, 247, 373
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BsuRI GGCC 4 cut(s) 37, 62, 120, 230
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 2 cut(s) 73, 151
Cfr13I GGNCC 3 cut(s) 229, 326, 371
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 1 cut(s) 55
CviAII CATG 2 cut(s) 76, 363
CviJI RGCY 7 cut(s) 37, 62, 120, 213, 230, 338, 344
CviKI_1 RGCY 7 cut(s) 37, 62, 120, 213, 230, 338, 344
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 350
EaeI YGGCCR 2 cut(s) 60, 118
Eco47I GGWCC 2 cut(s) 326, 371
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 2 cut(s) 79, 366
FaiI YATR 5 cut(s) 21, 77, 273, 321, 364
FaqI GGGAC 1 cut(s) 357
FatI CATG 2 cut(s) 75, 362
FokI GGATG 1 cut(s) 128
FspBI CTAG 1 cut(s) 385
HaeIII GGCC 4 cut(s) 37, 62, 120, 230
HapII CCGG 1 cut(s) 158
Hin1II CATG 2 cut(s) 79, 366
HpaII CCGG 1 cut(s) 158
HphI GGTGA 2 cut(s) 104, 244
Hpy188I TCNGA 2 cut(s) 115, 282
Hpy188III TCNNGA 2 cut(s) 158, 190
HpyAV CCTTC 2 cut(s) 48, 259
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 350
Hsp92II CATG 2 cut(s) 79, 366
Kpn2I TCCGGA 1 cut(s) 157
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 7 cut(s) 59, 171, 175, 270, 315, 330, 342
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 1 cut(s) 385
MaeIII GTNAC 1 cut(s) 305
MluCI AATT 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 6 cut(s) 83, 88, 139, 154, 157, 317
MroI TCCGGA 1 cut(s) 157
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 79, 366
NlaIV GGNNCC 3 cut(s) 231, 247, 373
NmuCI GTSAC 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 3 cut(s) 231, 247, 373
PspPI GGNCC 3 cut(s) 229, 326, 371
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
Sau96I GGNCC 3 cut(s) 229, 326, 371
ScrFI CCNGG 1 cut(s) 330
SetI ASST 6 cut(s) 94, 99, 131, 215, 251, 334
SexAI ACCWGGT 1 cut(s) 328
SinI GGWCC 2 cut(s) 326, 371
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 1 cut(s) 103
SspMI CTAG 1 cut(s) 385
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 68
TasI AATT 1 cut(s) 103
TscAI CASTG 2 cut(s) 73, 151
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspRI CASTG 2 cut(s) 73, 151
VpaK11BI GGWCC 2 cut(s) 326, 371
XapI RAATTY 1 cut(s) 103
XspI CTAG 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.