Rmu_sc0009946.1_g000009

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009946.1
OG0004413
Physical Location & Seq
Forward (+)
36960 .. 37349
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009946.1_g000009.1.cds

Sequence Viewer

Length: 390 bp
atgtttcagcaccaatcgactggacagtggtgggttatgttgcaagatcaagcattaggatactggccagacaaaatcttaccaagtctgagaggcagtgctgacgcggttagctggggtggtgaaatttacaattcagaaattggaggtcgtcacacatcaactcagatggggagtggtcatttcccgagtgaaggctatggcaaagcaagctattttcgaaatattcagtacttggccaactctggaaagtttgtagatgcacaaggccttataccctatgtcacaaagcctccatgctacaaccttattatgaaaaataaagccccaggatttggaaaccatttctattatgggggatttggatattcagaaagatgccctaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

129

Amino Acids

14.44

Weight (kDa)

8.87

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 335
AccII CGCG 1 cut(s) 107
AciI CCGC 1 cut(s) 107
AcoI YGGCCR 2 cut(s) 65, 237
AcsI RAATTY 1 cut(s) 126
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 194, 335
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 2 cut(s) 114, 213
AluI AGCT 2 cut(s) 114, 213
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 3 cut(s) 65, 237, 268
ApoI RAATTY 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 134
AsuII TTCGAA 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 187
BalI TGGCCA 2 cut(s) 67, 239
BccI CCATC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 330
BciVI GTATCC 1 cut(s) 53
BfuI GTATCC 1 cut(s) 53
BmcAI AGTACT 1 cut(s) 233
Bme1390I CCNGG 1 cut(s) 330
BmeT110I CYCGRG 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 250, 368
Bpu14I TTCGAA 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 277, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 335
Bse1I ACTGG 2 cut(s) 25, 68
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 328
BseLI CCNNNNNNNGG 2 cut(s) 194, 335
BseMII CTCAG 2 cut(s) 80, 179
BseNI ACTGG 2 cut(s) 25, 68
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 3 cut(s) 67, 239, 270
BsiHKCI CYCGRG 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 194, 335
BsnI GGCC 3 cut(s) 67, 239, 270
BsoBI CYCGRG 1 cut(s) 187
Bsp119I TTCGAA 1 cut(s) 220
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 1 cut(s) 107
BspANI GGCC 3 cut(s) 67, 239, 270
BspCNI CTCAG 2 cut(s) 81, 178
BspFNI CGCG 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 220
BsrI ACTGG 2 cut(s) 25, 68
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 27
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 89, 165
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BstUI CGCG 1 cut(s) 107
BstXI CCANNNNNNTGG 1 cut(s) 20
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 3 cut(s) 67, 239, 270
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 2 cut(s) 32, 103
Cac8I GCNNGC 1 cut(s) 211
CseI GACGC 1 cut(s) 113
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 297
CviJI RGCY 8 cut(s) 67, 114, 198, 213, 239, 270, 292, 326
CviKI_1 RGCY 8 cut(s) 67, 114, 198, 213, 239, 270, 292, 326
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 2 cut(s) 89, 165
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 65, 237
Eco147I AGGCCT 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 328
FaeI CATG 1 cut(s) 300
FaiI YATR 7 cut(s) 38, 201, 275, 282, 298, 314, 354
FatI CATG 1 cut(s) 296
GsaI CCCAGC 1 cut(s) 118
HaeIII GGCC 3 cut(s) 67, 239, 270
HgaI GACGC 1 cut(s) 113
Hin1II CATG 1 cut(s) 300
HphI GGTGA 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 90, 139, 168, 373
Hpy188III TCNNGA 2 cut(s) 187, 246
HpyAV CCTTC 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4V TGCA 2 cut(s) 43, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpyF3I CTNAG 2 cut(s) 89, 165
Hsp92II CATG 1 cut(s) 300
Kzo9I GATC 1 cut(s) 46
LpnPI CCDG 7 cut(s) 6, 49, 81, 100, 231, 315, 342
LweI GCATC 2 cut(s) 250, 368
MaeIII GTNAC 2 cut(s) 152, 283
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MlsI TGGCCA 2 cut(s) 67, 239
MluCI AATT 3 cut(s) 126, 133, 141
MluNI TGGCCA 2 cut(s) 67, 239
MnlI CCTC 3 cut(s) 86, 140, 303
Mox20I TGGCCA 2 cut(s) 67, 239
MscI TGGCCA 2 cut(s) 67, 239
Msp20I TGGCCA 2 cut(s) 67, 239
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 210
NdeII GATC 1 cut(s) 46
NlaIII CATG 1 cut(s) 300
NmuCI GTSAC 2 cut(s) 152, 283
NspV TTCGAA 1 cut(s) 220
PceI AGGCCT 1 cut(s) 270
PflMI CCANNNNNTGG 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 1 cut(s) 328
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
Sau3AI GATC 1 cut(s) 46
ScaI AGTACT 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 330
SetI ASST 4 cut(s) 116, 151, 215, 309
SfaNI GCATC 2 cut(s) 250, 368
SfuI TTCGAA 1 cut(s) 220
Sse9I AATT 3 cut(s) 126, 133, 141
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 1 cut(s) 107
SspI AATATT 1 cut(s) 226
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 27
TaqI TCGA 2 cut(s) 17, 220
TasI AATT 3 cut(s) 126, 133, 141
TatI WGTACW 1 cut(s) 231
TscAI CASTG 2 cut(s) 32, 103
TseFI GTSAC 2 cut(s) 152, 283
Tsp45I GTSAC 2 cut(s) 152, 283
TspDTI ATGAA 1 cut(s) 329
TspRI CASTG 2 cut(s) 32, 103
Van91I CCANNNNNTGG 1 cut(s) 335
XapI RAATTY 1 cut(s) 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.