Rw2G034570

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004413
Physical Location & Seq
Reverse (-)
56884360 .. 56887851
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G034570.1

Sequence Viewer

Length: 885 bp
ATGGAAATTCTGAATCCGCCAAGTCCGTATCCAAGTGGACTTAATGAAACAGAAAGTTCTCATCAAGATGACGAGCAAGTACTGAAATCTTGGTTATTGAATGAAGAGTGCCCAGAAGGAACAATCCCCATTCTTCGAACTCAGGCTTTTAATAGTTCTTTTCCTAAATCTAGCCCACCTTTCAATGGAAGCAACGTTGAGTTCACGCCTGCATCTGGTCATGAGTACGCCGTAGCTAGTCTTTCAGGTGGCCAATATTACGGAGCACACGCATCTGTGAACGTATGGAGCCCTTTATCAGTTTTTGAGGAGAATAGTATATCCCAGATATGGGTAGCGGGAGGTCATGAACAAGCACGAAGTACTATTAATTCGGCTGACCAGCAAACAAGGTTCTTCATATTCTGGACAGGCCATGGTTACCAAGATGGTTGCTACAATCTTCAATGTCCTGGTTTCGTCCAAGTAAACAGAAAGTTTGCCCTAGGTTTACCCATTAAACCTGTTTCAATCTACAACGGCAAACAATTTGAAATATTCATAAGTATATACAAGGATACTGGCCAGACAAATCTACCAAGTCTGAGAGGCAGTGCTGACGCGGTTAGCTGGGGTGGTGAAATTTACAATTCAGAAATTGGAGGTCGTCACACATCAACTCAGATGGGGAGTGGTCATTTCCCGAGTGAAGGCTATGGCAAAGCAAGCTATTTTCGAAATATTCAGTACTTGGCCAACTCTGGAAAGTTTGTAGATGCACAAGGGCTTATACCCTATGTCACAAAGCCTCCATGCTACAACCTTATTATGAAAAATAAAGCCCCAGGATTTGGAAACCATTTCTATTATGGGGGATTTGGATATTCAGAAAGATGCCCTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

294

Amino Acids

32.41

Weight (kDa)

6.06

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin PF03080 104 - 285 1.1e-45 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 830
AccII CGCG 1 cut(s) 602
AciI CCGC 3 cut(s) 17, 338, 602
AclI AACGTT 1 cut(s) 195
AcoI YGGCCR 3 cut(s) 250, 562, 732
AcsI RAATTY 2 cut(s) 6, 621
AfaI GTAC 4 cut(s) 81, 227, 364, 728
AfiI CCNNNNNNNGG 6 cut(s) 185, 215, 330, 331, 689, 830
AgsI TTSAA 5 cut(s) 100, 184, 446, 510, 533
AjnI CCWGG 2 cut(s) 451, 823
AluBI AGCT 3 cut(s) 236, 609, 708
AluI AGCT 3 cut(s) 236, 609, 708
Alw21I GWGCWC 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 682
AoxI GGCC 4 cut(s) 250, 412, 562, 732
ApoI RAATTY 2 cut(s) 6, 621
AseI ATTAAT 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 484
AsuHPI GGTGA 1 cut(s) 629
AsuII TTCGAA 2 cut(s) 136, 715
AvaI CYCGRG 1 cut(s) 682
AvrII CCTAGG 1 cut(s) 484
BaeGI GKGCMC 1 cut(s) 113
BalI TGGCCA 3 cut(s) 252, 564, 734
BanII GRGCYC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 268
BccI CCATC 2 cut(s) 422, 658
BceAI ACGGC 2 cut(s) 215, 535
BciT130I CCWGG 2 cut(s) 453, 825
BciVI GTATCC 2 cut(s) 39, 550
BfaI CTAG 3 cut(s) 171, 237, 485
BfuI GTATCC 2 cut(s) 39, 550
BlnI CCTAGG 1 cut(s) 484
BmcAI AGTACT 3 cut(s) 81, 364, 728
Bme1390I CCNGG 2 cut(s) 453, 825
BmeT110I CYCGRG 1 cut(s) 682
BmiI GGNNCC 1 cut(s) 290
BmrFI CCNGG 2 cut(s) 453, 825
BmsI GCATC 4 cut(s) 221, 281, 745, 863
Bpu14I TTCGAA 2 cut(s) 136, 715
BsaJI CCNNGG 3 cut(s) 415, 484, 823
BsaXI ACNNNNNCTCC 2 cut(s) 772, 802
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 215, 330, 331, 689, 830
Bse1I ACTGG 1 cut(s) 565
BseBI CCWGG 2 cut(s) 453, 825
BseDI CCNNGG 3 cut(s) 415, 484, 823
BseLI CCNNNNNNNGG 6 cut(s) 185, 215, 330, 331, 689, 830
BseMII CTCAG 3 cut(s) 155, 575, 674
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 113
BseYI CCCAGC 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 4 cut(s) 252, 414, 564, 734
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 682
BslI CCNNNNNNNGG 6 cut(s) 185, 215, 330, 331, 689, 830
BsnI GGCC 4 cut(s) 252, 414, 564, 734
BsoBI CYCGRG 1 cut(s) 682
Bsp119I TTCGAA 2 cut(s) 136, 715
Bsp1286I GDGCHC 3 cut(s) 113, 268, 293
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 3 cut(s) 17, 338, 602
BspANI GGCC 4 cut(s) 252, 414, 564, 734
BspCNI CTCAG 3 cut(s) 154, 576, 673
BspFNI CGCG 1 cut(s) 602
BspHI TCATGA 2 cut(s) 220, 346
BspLI GGNNCC 1 cut(s) 290
BspT104I TTCGAA 2 cut(s) 136, 715
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 3 cut(s) 415, 484, 823
BssT1I CCWWGG 2 cut(s) 415, 484
Bst2UI CCWGG 2 cut(s) 453, 825
Bst6I CTCTTC 1 cut(s) 99
BstBI TTCGAA 2 cut(s) 136, 715
BstC8I GCNNGC 2 cut(s) 210, 706
BstDEI CTNAG 3 cut(s) 141, 584, 660
BstDSI CCRYGG 1 cut(s) 415
BstEII GGTNACC 1 cut(s) 419
BstFNI CGCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 1 cut(s) 705
BstNI CCWGG 2 cut(s) 453, 825
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 2 cut(s) 451, 823
BstSLI GKGCMC 1 cut(s) 113
BstUI CGCG 1 cut(s) 602
BsuI GTATCC 2 cut(s) 39, 550
BsuRI GGCC 4 cut(s) 252, 414, 564, 734
BtgI CCRYGG 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 1 cut(s) 598
Cac8I GCNNGC 2 cut(s) 210, 706
CciI TCATGA 2 cut(s) 220, 346
CseI GACGC 1 cut(s) 608
Csp6I GTAC 4 cut(s) 80, 226, 363, 727
CviAII CATG 4 cut(s) 221, 347, 416, 792
CviQI GTAC 4 cut(s) 80, 226, 363, 727
DdeI CTNAG 3 cut(s) 141, 584, 660
EaeI YGGCCR 3 cut(s) 250, 562, 732
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 415, 484
Eco24I GRGCYC 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 682
Eco91I GGTNACC 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 451, 823
EcoT14I CCWWGG 2 cut(s) 415, 484
EcoT38I GRGCYC 1 cut(s) 293
ErhI CCWWGG 2 cut(s) 415, 484
FaeI CATG 4 cut(s) 224, 350, 419, 795
FatI CATG 4 cut(s) 220, 346, 415, 791
FauI CCCGC 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 293
FspBI CTAG 3 cut(s) 171, 237, 485
GsaI CCCAGC 1 cut(s) 613
HaeIII GGCC 4 cut(s) 252, 414, 564, 734
HgaI GACGC 1 cut(s) 608
Hin1II CATG 4 cut(s) 224, 350, 419, 795
HinfI GANTC 1 cut(s) 13
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 5 cut(s) 38, 204, 280, 469, 491
Hpy188I TCNGA 5 cut(s) 12, 585, 634, 663, 868
Hpy188III TCNNGA 6 cut(s) 65, 221, 347, 406, 682, 741
Hpy8I GTNNAC 5 cut(s) 38, 204, 280, 469, 491
HpyAV CCTTC 2 cut(s) 110, 683
HpyCH4IV ACGT 2 cut(s) 195, 282
HpyCH4V TGCA 2 cut(s) 212, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 705
HpyF3I CTNAG 3 cut(s) 141, 584, 660
HpySE526I ACGT 2 cut(s) 195, 282
Hsp92II CATG 4 cut(s) 224, 350, 419, 795
LmnI GCTCC 2 cut(s) 263, 288
LweI GCATC 4 cut(s) 221, 281, 745, 863
MaeI CTAG 3 cut(s) 171, 237, 485
MaeII ACGT 2 cut(s) 195, 282
MaeIII GTNAC 3 cut(s) 419, 647, 778
MboII GAAGA 4 cut(s) 116, 125, 388, 434
MhlI GDGCHC 3 cut(s) 113, 268, 293
MlsI TGGCCA 3 cut(s) 252, 564, 734
MluCI AATT 6 cut(s) 6, 370, 527, 621, 628, 636
MluNI TGGCCA 3 cut(s) 252, 564, 734
MnlI CCTC 5 cut(s) 301, 335, 581, 635, 798
Mox20I TGGCCA 3 cut(s) 252, 564, 734
MscI TGGCCA 3 cut(s) 252, 564, 734
MseI TTAA 4 cut(s) 42, 150, 369, 498
MslI CAYNNNNRTG 1 cut(s) 66
Msp20I TGGCCA 3 cut(s) 252, 564, 734
MspR9I CCNGG 2 cut(s) 453, 825
MvaI CCWGG 2 cut(s) 453, 825
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 705
NcoI CCATGG 1 cut(s) 415
NlaIII CATG 4 cut(s) 224, 350, 419, 795
NlaIV GGNNCC 1 cut(s) 290
NmuCI GTSAC 2 cut(s) 647, 778
NspV TTCGAA 2 cut(s) 136, 715
PagI TCATGA 2 cut(s) 220, 346
PfeI GAWTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 830
PshBI ATTAAT 1 cut(s) 369
Psp1406I AACGTT 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 451, 823
PspEI GGTNACC 1 cut(s) 419
PspFI CCCAGC 1 cut(s) 609
PspGI CCWGG 2 cut(s) 451, 823
PspN4I GGNNCC 1 cut(s) 290
RsaI GTAC 4 cut(s) 81, 227, 364, 728
RsaNI GTAC 4 cut(s) 80, 226, 363, 727
RseI CAYNNNNRTG 1 cut(s) 66
SaqAI TTAA 4 cut(s) 42, 150, 369, 498
ScaI AGTACT 3 cut(s) 81, 364, 728
ScrFI CCNGG 2 cut(s) 453, 825
SduI GDGCHC 3 cut(s) 113, 268, 293
SfaNI GCATC 4 cut(s) 221, 281, 745, 863
SfuI TTCGAA 2 cut(s) 136, 715
SmiMI CAYNNNNRTG 1 cut(s) 66
Sse9I AATT 6 cut(s) 6, 370, 527, 621, 628, 636
SsiI CCGC 3 cut(s) 17, 338, 602
SspI AATATT 3 cut(s) 257, 537, 721
SspMI CTAG 3 cut(s) 171, 237, 485
StyD4I CCNGG 2 cut(s) 451, 823
StyI CCWWGG 2 cut(s) 415, 484
TaiI ACGT 2 cut(s) 198, 285
TaqI TCGA 2 cut(s) 136, 715
TasI AATT 6 cut(s) 6, 370, 527, 621, 628, 636
TatI WGTACW 3 cut(s) 79, 362, 726
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 4 cut(s) 42, 150, 369, 498
Tru9I TTAA 4 cut(s) 42, 150, 369, 498
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 647, 778
Tsp45I GTSAC 2 cut(s) 647, 778
TspDTI ATGAA 6 cut(s) 60, 117, 363, 388, 529, 824
TspGWI ACGGA 2 cut(s) 15, 276
TspRI CASTG 1 cut(s) 598
Van91I CCANNNNNTGG 1 cut(s) 830
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 2 cut(s) 6, 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XmaJI CCTAGG 1 cut(s) 484
XspI CTAG 3 cut(s) 171, 237, 485
ZrmI AGTACT 3 cut(s) 81, 364, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.