Rorug02G0369300

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004413
Physical Location & Seq
Forward (+)
47106440 .. 47109188
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0369300.1

Sequence Viewer

Length: 1038 bp
ATGGCTCCTCCTCCAGGGATTGTTGTCACCCTTTTGCTTCTTCTTGCTCTGCAGAGTTATGGGTTCGTTCTTTCTTTTGCTTCCACAAATTGTCCTCAAGAGTCAAGGCTCAAGCAGAGTTCTGGGTTCTTGAAGGCTTACTTGAAGGATTGCAAGATAGTAGTGTATGAATGGACAGGAAAGTGTAGGAGTTGCCAAGGCTCAGGATATGCTAGTTATTATAATAAAAGAGGGAAGAAAAATGTTTGCAAATGCATACCTTGCCAAGGAATCGATGGAACAGGAAGAATACACGAAGAAGAAATCAATTGGAGCTACATTGAGTTTGTTGATAACCAAGATGTGCTGGATTTGATTGAAAAGAAACCTGGAGGAGTAATTTCCCTCTTGGATGAAGCCTGTATGTTTCCTAAATCCACACATGAGACGTTCGCTCAGAAGTTATACCAGACATTCAAAAACAACAAGCGGTTTATCAAACCTAAGCTTTCTCGAACTAGTTTTACCATCTCTCACTACGCAGGAGAGGTGACTTATCTAGCTGATCAGTTCATTGATAAAAACAAAGATTATGTGGTGGCGGAACACCAGGATCTATTGACAGCCTCCAAATGCCCTTTTGTGGCAGGGCTATTTCCTCCACTTCCAGAGGAATCATCCAAGTCTTCAAAATTTTCATCCATTGGATCACGCTTCAAGTTACAACTTCAATCTTTGATGGAGACCTTGAACTCAATGGAACCTCACTCTATCAGATGTGTGAAGCCAAACAATGTCCTCAAGCCTGCTATTTTTGAGAACTTCAACATAATACAACAATTACGTTATGGTGGTGTTCTTGAGGCGATTAGGATCAGCTGTGCTGGATATCCTACCAGACGTACATTTTATGAGTTTCTTAACCGCTTTGTGGTTCTTGCTCCTGATGTTTTGGAAGGAAACTCTGACGACAAGGTTGCATGTCAAATGATCCTTGATAAGATGGGATTGACTGGTTATCAGGTAGGCAAGACAAAGGTCTTCCTAAGAGCTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

345

Amino Acids

38.81

Weight (kDa)

8.93

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_head PF00063 99 - 343 2e-74 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AciI CCGC 3 cut(s) 469, 581, 904
AclWI GGATC 4 cut(s) 600, 694, 860, 964
AcsI RAATTY 1 cut(s) 671
AfaI GTAC 1 cut(s) 883
AfiI CCNNNNNNNGG 4 cut(s) 14, 266, 622, 910
AgsI TTSAA 9 cut(s) 133, 145, 359, 457, 669, 697, 710, 730, 805
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 3 cut(s) 13, 367, 588
AluBI AGCT 5 cut(s) 315, 487, 542, 858, 1031
AluI AGCT 5 cut(s) 315, 487, 542, 858, 1031
Alw26I GTCTC 2 cut(s) 419, 716
AlwI GGATC 4 cut(s) 600, 694, 860, 964
ApoI RAATTY 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 19, 541
BbsI GAAGAC 2 cut(s) 657, 1012
BccI CCATC 4 cut(s) 269, 515, 712, 976
BcgI CGANNNNNNTGC 2 cut(s) 938, 972
BciT130I CCWGG 3 cut(s) 15, 369, 590
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 419, 716
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 3 cut(s) 213, 498, 539
BfmI CTRYAG 1 cut(s) 50
Bme1390I CCNGG 3 cut(s) 15, 369, 590
BmiI GGNNCC 2 cut(s) 6, 741
BmrFI CCNGG 3 cut(s) 15, 369, 590
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 2 cut(s) 657, 1012
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 2 cut(s) 202, 483
BpuEI CTTGAG 4 cut(s) 81, 95, 764, 860
Bsa29I ATCGAT 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 3 cut(s) 14, 196, 265
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 266, 622, 910
Bse1I ACTGG 1 cut(s) 997
Bse8I GATNNNNATC 1 cut(s) 851
BseBI CCWGG 3 cut(s) 15, 369, 590
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 3 cut(s) 14, 196, 265
BseGI GGATG 3 cut(s) 397, 656, 677
BseJI GATNNNNATC 1 cut(s) 851
BseLI CCNNNNNNNGG 4 cut(s) 14, 266, 622, 910
BseMII CTCAG 2 cut(s) 216, 449
BseNI ACTGG 1 cut(s) 997
BseRI GAGGAG 1 cut(s) 387
BshVI ATCGAT 1 cut(s) 273
BslI CCNNNNNNNGG 4 cut(s) 14, 266, 622, 910
BsmAI GTCTC 2 cut(s) 419, 716
BsmBI CGTCTC 1 cut(s) 419
Bso31I GGTCTC 1 cut(s) 716
Bsp143I GATC 5 cut(s) 544, 592, 686, 852, 969
BspACI CCGC 3 cut(s) 469, 581, 904
BspCNI CTCAG 2 cut(s) 215, 448
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 6, 741
BspMAI CTGCAG 1 cut(s) 54
BspPI GGATC 4 cut(s) 600, 694, 860, 964
BspTNI GGTCTC 1 cut(s) 716
BsrI ACTGG 1 cut(s) 997
BssECI CCNNGG 3 cut(s) 14, 196, 265
BssMI GATC 5 cut(s) 544, 592, 686, 852, 969
BssT1I CCWWGG 2 cut(s) 196, 265
Bst2UI CCWGG 3 cut(s) 15, 369, 590
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 4 cut(s) 202, 435, 483, 1025
BstENI CCTNNNNNAGG 2 cut(s) 12, 264
BstF5I GGATG 3 cut(s) 397, 656, 677
BstKTI GATC 5 cut(s) 547, 595, 689, 855, 972
BstMAI GTCTC 2 cut(s) 419, 716
BstMBI GATC 5 cut(s) 544, 592, 686, 852, 969
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 3 cut(s) 15, 369, 590
BstNSI RCATGY 1 cut(s) 963
BstPAI GACNNNNGTC 1 cut(s) 1016
BstSCI CCNGG 3 cut(s) 13, 367, 588
BstSFI CTRYAG 1 cut(s) 50
BstV2I GAAGAC 2 cut(s) 657, 1012
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
Bsu15I ATCGAT 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 3 cut(s) 397, 656, 677
Cac8I GCNNGC 1 cut(s) 786
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 882
CviAII CATG 2 cut(s) 422, 960
CviQI GTAC 1 cut(s) 882
DdeI CTNAG 4 cut(s) 202, 435, 483, 1025
DpnI GATC 5 cut(s) 546, 594, 688, 854, 971
DpnII GATC 5 cut(s) 544, 592, 686, 852, 969
EciI GGCGGA 1 cut(s) 596
Eco130I CCWWGG 2 cut(s) 196, 265
Eco31I GGTCTC 1 cut(s) 716
Eco32I GATATC 1 cut(s) 869
EcoNI CCTNNNNNAGG 2 cut(s) 12, 264
EcoRII CCWGG 3 cut(s) 13, 367, 588
EcoRV GATATC 1 cut(s) 869
EcoT14I CCWWGG 2 cut(s) 196, 265
EcoT22I ATGCAT 1 cut(s) 257
ErhI CCWWGG 2 cut(s) 196, 265
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 2 cut(s) 425, 963
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 2 cut(s) 421, 959
FbaI TGATCA 1 cut(s) 544
FokI GGATG 3 cut(s) 404, 643, 664
FspBI CTAG 3 cut(s) 213, 498, 539
GsuI CTGGAG 1 cut(s) 390
Hin1II CATG 2 cut(s) 425, 963
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 101, 270, 653
HphI GGTGA 2 cut(s) 19, 541
Hpy188I TCNGA 3 cut(s) 438, 755, 946
Hpy188III TCNNGA 7 cut(s) 98, 130, 204, 492, 647, 839, 923
HpyAV CCTTC 3 cut(s) 127, 139, 929
HpyCH4IV ACGT 3 cut(s) 428, 823, 880
HpyCH4V TGCA 5 cut(s) 52, 153, 249, 255, 959
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 4 cut(s) 202, 435, 483, 1025
HpySE526I ACGT 3 cut(s) 428, 823, 880
Hsp92II CATG 2 cut(s) 425, 963
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 5 cut(s) 544, 592, 686, 852, 969
LmnI GCTCC 3 cut(s) 10, 312, 925
MaeI CTAG 3 cut(s) 213, 498, 539
MaeII ACGT 3 cut(s) 428, 823, 880
MaeIII GTNAC 3 cut(s) 25, 529, 699
MalI GATC 5 cut(s) 546, 594, 688, 854, 971
MboI GATC 5 cut(s) 544, 592, 686, 852, 969
MboII GAAGA 7 cut(s) 32, 247, 297, 308, 311, 657, 1012
MfeI CAATTG 1 cut(s) 307
MflI RGATCY 1 cut(s) 592
MluCI AATT 5 cut(s) 88, 307, 378, 671, 818
MlyI GAGTC 1 cut(s) 110
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 1 cut(s) 900
MspA1I CMGCKG 1 cut(s) 858
MspR9I CCNGG 3 cut(s) 15, 369, 590
MunI CAATTG 1 cut(s) 307
MvaI CCWGG 3 cut(s) 15, 369, 590
MwoI GCNNNNNNNGC 1 cut(s) 261
NdeII GATC 5 cut(s) 544, 592, 686, 852, 969
NlaIII CATG 2 cut(s) 425, 963
NlaIV GGNNCC 2 cut(s) 6, 741
NmuCI GTSAC 2 cut(s) 25, 529
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 1 cut(s) 963
PfeI GAWTC 2 cut(s) 270, 653
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PshAI GACNNNNGTC 1 cut(s) 1016
PsiI TTATAA 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 13, 367, 588
PspGI CCWGG 3 cut(s) 13, 367, 588
PspN4I GGNNCC 2 cut(s) 6, 741
PsrI GAACNNNNNNTAC 2 cut(s) 487, 519
PstI CTGCAG 1 cut(s) 54
PsuI RGATCY 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 858
RsaI GTAC 1 cut(s) 883
RsaNI GTAC 1 cut(s) 882
SaqAI TTAA 1 cut(s) 900
Sau3AI GATC 5 cut(s) 544, 592, 686, 852, 969
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 15, 369, 590
SfcI CTRYAG 1 cut(s) 50
SmlI CTYRAG 4 cut(s) 96, 110, 779, 839
SmoI CTYRAG 4 cut(s) 96, 110, 779, 839
SpeI ACTAGT 1 cut(s) 497
Sse9I AATT 5 cut(s) 88, 307, 378, 671, 818
SsiI CCGC 3 cut(s) 469, 581, 904
SspMI CTAG 3 cut(s) 213, 498, 539
StyD4I CCNGG 3 cut(s) 13, 367, 588
StyI CCWWGG 2 cut(s) 196, 265
TaiI ACGT 3 cut(s) 431, 826, 883
TaqI TCGA 2 cut(s) 273, 493
TasI AATT 5 cut(s) 88, 307, 378, 671, 818
TfiI GAWTC 2 cut(s) 270, 653
Tru1I TTAA 1 cut(s) 900
Tru9I TTAA 1 cut(s) 900
TseFI GTSAC 2 cut(s) 25, 529
Tsp45I GTSAC 2 cut(s) 25, 529
TspDTI ATGAA 4 cut(s) 183, 408, 541, 666
XagI CCTNNNNNAGG 2 cut(s) 12, 264
XapI RAATTY 1 cut(s) 671
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XspI CTAG 3 cut(s) 213, 498, 539
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.