AT3G13062

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002322
Physical Location & Seq
Forward (+)
4184340 .. 4187453
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13062.3

Sequence Viewer

Length: 1212 bp
ATGAATAGTGGTGATACCTTATCGACGTTAGCTCAATTTATCTCCTCTTCCGAGAGATTTTTCCGGCGAGCTGACGGTGATGTAGTCTTTTGTTATTGCTGGGCCACCGTGCTCGCACTTGTCTTCATTCTTCTCTACCAATTCTTCCTTTCCCGGAAATTCCGATTCTTCAGTCATGCTTCTTCTTCTTCCCCTGTCGTCTCGGTTTCTCAATCTCTCAGCTCCCAATCAGGAATATCTACTACACTTGTTTCTGATGAAGATCTAAAGGGTCTGATTCAAAAATTGGGGGAAAGAAGTGAGGATGCTGAGATATGGGAAGATGTTATTAAGAAAAGCAACCCTCGTATCTCCTACACTGCTAAATGCTGCAAACCAACAGATGGTAGTCCCATGAAGTATCTGAGTACAACTGTGTTTGAGGATTGCTCCCCAGAGGTTTTGAGGGACTTTTACATGGACAATGAATACAGGAAACAATGGGACAAGACTGTGGTTGAGCATGAGCAATTGCAGGTTGACAGTAATAGTGGGATTGAGATTGGTCGCACTATAAAAAAGTTTCCCTTGTTGACTCCAAGGGAGTATGTATTAGCGTGGAAATTGTGGGAGGGGAAGGATAAGTTTTACTGTTTCATCAAGGAATGTGATCACAATATGGTGCCGCAACAGAGGAAGTATGTACGTGTAAGTTATTTCAGATCTGGTTGGCGAATTAGGAAAGTTCCTGGTAGGAACGCTTGTGAGATACACATGGTTCACCAGGAGGATGCTGGATTAAATGTTGAGATGGCAAAGCTTGCTTTCTCAAGAGGCATATGGAGTTACGTTTGTAAGATGGAAAATGCACTTCGCAAGTACATTGCAACCAGTCATCGACCCCAAGGGCCCACATTATCTGCTGTAAGCTTAATGAAAAAGATTCCATCGGAGTTAGAAAGTCAGACAGACGACATTACAAACTCCTCGGGAACTACCACTAGTGGTATGCACACAGGTGAAGGAGCCAAACGGAAGAAACTGTTGAGAAAGCCTTCAAAGAAACTGATAGCAAATGGTATGTTACTTGTGGGCGGTGCCGTTGGTGGTGCAATCTGCCTGTCTCGTGGTCACTCCGCCTTAGGGGCAAAGGTCGCGTTGGCTTACTTCTTGTCGAAGATGAGAAAGCGTGGAGCTCAACTGAGTCAAACTTCCCAAAACGCTGGTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

403

Amino Acids

45.31

Weight (kDa)

9.39

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 98 - 265 5.8e-12 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccB1I GGYRCC 2 cut(s) 661, 1076
AccB7I CCANNNNNTGG 1 cut(s) 383
AccII CGCG 1 cut(s) 1136
AciI CCGC 3 cut(s) 665, 1074, 1116
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 154
AfaI GTAC 3 cut(s) 409, 684, 860
AfiI CCNNNNNNNGG 2 cut(s) 383, 1122
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 2 cut(s) 281, 1038
AhlI ACTAGT 1 cut(s) 980
AjnI CCWGG 2 cut(s) 727, 762
AleI CACNNNNGTG 1 cut(s) 996
AluBI AGCT 6 cut(s) 32, 71, 222, 799, 909, 1175
AluI AGCT 6 cut(s) 32, 71, 222, 799, 909, 1175
Alw21I GWGCWC 2 cut(s) 114, 1177
Alw26I GTCTC 2 cut(s) 205, 1107
Ama87I CYCGRG 1 cut(s) 967
AoxI GGCC 2 cut(s) 102, 887
ApaI GGGCCC 1 cut(s) 891
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 1033
AspS9I GGNCC 3 cut(s) 102, 887, 888
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 4 cut(s) 23, 89, 752, 1010
AvaI CYCGRG 1 cut(s) 967
AxyI CCTNAGG 1 cut(s) 1120
BaeGI GKGCMC 1 cut(s) 891
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BanI GGYRCC 2 cut(s) 661, 1076
BanII GRGCYC 2 cut(s) 891, 1177
BauI CACGAG 1 cut(s) 1104
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 2 cut(s) 114, 1177
BbvI GCAGC 1 cut(s) 356
BccI CCATC 4 cut(s) 377, 784, 832, 934
BceAI ACGGC 1 cut(s) 1064
BciT130I CCWGG 2 cut(s) 729, 764
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 205, 1107
BcuI ACTAGT 1 cut(s) 980
BfaI CTAG 1 cut(s) 981
BfuAI ACCTGC 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 1124
BglII AGATCT 2 cut(s) 262, 701
BisI GCNGC 2 cut(s) 370, 665
BlsI GCNGC 2 cut(s) 371, 666
Bme1390I CCNGG 3 cut(s) 154, 729, 764
BmeT110I CYCGRG 1 cut(s) 967
BmgT120I GGNCC 3 cut(s) 102, 887, 888
BmiI GGNNCC 4 cut(s) 663, 889, 1006, 1078
BmrFI CCNGG 3 cut(s) 154, 729, 764
BmsI GCATC 2 cut(s) 295, 760
BpiI GAAGAC 1 cut(s) 115
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpuEI CTTGAG 1 cut(s) 793
BpuMI CCSGG 1 cut(s) 154
BsaAI YACGTR 1 cut(s) 686
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 3 cut(s) 578, 883, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 1122
Bse1I ACTGG 1 cut(s) 870
Bse21I CCTNAGG 1 cut(s) 1120
Bse3DI GCAATG 1 cut(s) 861
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 729, 764
BseDI CCNNGG 3 cut(s) 578, 883, 966
BseGI GGATG 2 cut(s) 310, 775
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 2 cut(s) 383, 1122
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 4 cut(s) 232, 300, 395, 1172
BseNI ACTGG 1 cut(s) 870
BseRI GAGGAG 2 cut(s) 34, 955
BseSI GKGCMC 1 cut(s) 891
BseXI GCAGC 1 cut(s) 356
BseYI CCCAGC 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 2 cut(s) 104, 889
BshNI GGYRCC 2 cut(s) 661, 1076
BsiHKAI GWGCWC 2 cut(s) 114, 1177
BsiHKCI CYCGRG 1 cut(s) 967
BsiSI CCGG 2 cut(s) 64, 154
BslFI GGGAC 3 cut(s) 375, 461, 497
BslI CCNNNNNNNGG 2 cut(s) 383, 1122
BsmAI GTCTC 2 cut(s) 205, 1107
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 3 cut(s) 375, 461, 497
BsnI GGCC 2 cut(s) 104, 889
BsoBI CYCGRG 1 cut(s) 967
Bsp120I GGGCCC 1 cut(s) 887
Bsp1286I GDGCHC 3 cut(s) 114, 891, 1177
Bsp143I GATC 3 cut(s) 262, 649, 701
BspACI CCGC 3 cut(s) 665, 1074, 1116
BspANI GGCC 2 cut(s) 104, 889
BspCNI CTCAG 4 cut(s) 231, 301, 396, 1173
BspFNI CGCG 1 cut(s) 1136
BspLI GGNNCC 4 cut(s) 663, 889, 1006, 1078
BspMI ACCTGC 1 cut(s) 505
BspT107I GGYRCC 2 cut(s) 661, 1076
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 1 cut(s) 870
BssECI CCNNGG 3 cut(s) 578, 883, 966
BssMI GATC 3 cut(s) 262, 649, 701
BssSI CACGAG 1 cut(s) 1104
BssT1I CCWWGG 2 cut(s) 578, 883
Bst2BI CACGAG 1 cut(s) 1104
Bst2UI CCWGG 2 cut(s) 729, 764
Bst4CI ACNGT 7 cut(s) 77, 109, 415, 493, 524, 632, 1023
Bst6I CTCTTC 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 69, 114, 801
BstDEI CTNAG 5 cut(s) 218, 309, 404, 1120, 1181
BstF5I GGATG 2 cut(s) 310, 775
BstFNI CGCG 1 cut(s) 1136
BstKTI GATC 3 cut(s) 265, 652, 704
BstMAI GTCTC 2 cut(s) 205, 1107
BstMBI GATC 3 cut(s) 262, 649, 701
BstMWI GCNNNNNNNGC 3 cut(s) 800, 1124, 1133
BstNI CCWGG 2 cut(s) 729, 764
BstSCI CCNGG 3 cut(s) 152, 727, 762
BstSLI GKGCMC 1 cut(s) 891
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 1 cut(s) 356
BstV2I GAAGAC 1 cut(s) 115
BstX2I RGATCY 2 cut(s) 262, 701
BstXI CCANNNNNNTGG 1 cut(s) 1202
BstYI RGATCY 2 cut(s) 262, 701
Bsu36I CCTNAGG 1 cut(s) 1120
BsuRI GGCC 2 cut(s) 104, 889
BtsCI GGATG 2 cut(s) 310, 775
BtsI GCAGTG 1 cut(s) 357
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 3 cut(s) 69, 114, 801
Cfr13I GGNCC 3 cut(s) 102, 887, 888
Csp6I GTAC 3 cut(s) 408, 683, 859
CviAII CATG 5 cut(s) 176, 394, 457, 503, 754
CviQI GTAC 3 cut(s) 408, 683, 859
DdeI CTNAG 5 cut(s) 218, 309, 404, 1120, 1181
DpnI GATC 3 cut(s) 264, 651, 703
DpnII GATC 3 cut(s) 262, 649, 701
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 1105
Ecl136II GAGCTC 1 cut(s) 1175
Eco130I CCWWGG 2 cut(s) 578, 883
Eco24I GRGCYC 2 cut(s) 891, 1177
Eco53kI GAGCTC 1 cut(s) 1175
Eco57I CTGAAG 1 cut(s) 154
Eco81I CCTNAGG 1 cut(s) 1120
Eco88I CYCGRG 1 cut(s) 967
EcoICRI GAGCTC 1 cut(s) 1175
EcoO109I RGGNCCY 1 cut(s) 887
EcoRII CCWGG 2 cut(s) 727, 762
EcoT14I CCWWGG 2 cut(s) 578, 883
EcoT38I GRGCYC 2 cut(s) 891, 1177
ErhI CCWWGG 2 cut(s) 578, 883
Esp3I CGTCTC 1 cut(s) 205
FaeI CATG 5 cut(s) 179, 397, 460, 506, 757
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 3 cut(s) 375, 461, 497
FatI CATG 5 cut(s) 175, 393, 456, 502, 753
FauNDI CATATG 1 cut(s) 818
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 2 cut(s) 370, 665
FokI GGATG 2 cut(s) 317, 782
FriOI GRGCYC 2 cut(s) 891, 1177
Fsp4HI GCNGC 2 cut(s) 370, 665
FspBI CTAG 1 cut(s) 981
GluI GCNGC 2 cut(s) 370, 665
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 2 cut(s) 104, 889
HapII CCGG 2 cut(s) 64, 154
Hin1II CATG 5 cut(s) 179, 397, 460, 506, 757
HincII GTYRAC 2 cut(s) 520, 573
HindII GTYRAC 2 cut(s) 520, 573
HindIII AAGCTT 2 cut(s) 797, 907
HinfI GANTC 5 cut(s) 165, 277, 574, 922, 1183
HpaII CCGG 2 cut(s) 64, 154
HphI GGTGA 4 cut(s) 23, 89, 752, 1010
Hpy166II GTNNAC 3 cut(s) 520, 573, 760
Hpy188I TCNGA 8 cut(s) 52, 164, 256, 276, 405, 701, 931, 945
Hpy188III TCNNGA 3 cut(s) 231, 810, 969
Hpy8I GTNNAC 3 cut(s) 520, 573, 760
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 610, 995, 1044
HpyCH4III ACNGT 7 cut(s) 77, 109, 415, 493, 524, 632, 1023
HpyCH4IV ACGT 3 cut(s) 26, 685, 828
HpyCH4V TGCA 6 cut(s) 372, 514, 848, 866, 991, 1091
HpyF10VI GCNNNNNNNGC 3 cut(s) 800, 1124, 1133
HpyF3I CTNAG 5 cut(s) 218, 309, 404, 1120, 1181
HpySE526I ACGT 3 cut(s) 26, 685, 828
Hsp92II CATG 5 cut(s) 179, 397, 460, 506, 757
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 3 cut(s) 262, 649, 701
LmnI GCTCC 4 cut(s) 227, 434, 1004, 1172
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 2 cut(s) 295, 760
MaeI CTAG 1 cut(s) 981
MaeII ACGT 3 cut(s) 26, 685, 828
MaeIII GTNAC 3 cut(s) 824, 1062, 1109
MalI GATC 3 cut(s) 264, 651, 703
MboI GATC 3 cut(s) 262, 649, 701
MfeI CAATTG 1 cut(s) 509
MflI RGATCY 2 cut(s) 262, 701
MhlI GDGCHC 3 cut(s) 114, 891, 1177
MluCI AATT 7 cut(s) 35, 140, 158, 284, 509, 602, 714
MlyI GAGTC 2 cut(s) 568, 1192
MroXI GAANNNNTTC 1 cut(s) 1033
MseI TTAA 4 cut(s) 330, 779, 911, 1210
MslI CAYNNNNRTG 1 cut(s) 996
MspI CCGG 2 cut(s) 64, 154
MspR9I CCNGG 3 cut(s) 154, 729, 764
MunI CAATTG 1 cut(s) 509
MvaI CCWGG 2 cut(s) 729, 764
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 3 cut(s) 800, 1124, 1133
NciI CCSGG 1 cut(s) 154
NdeI CATATG 1 cut(s) 818
NdeII GATC 3 cut(s) 262, 649, 701
NlaIII CATG 5 cut(s) 179, 397, 460, 506, 757
NlaIV GGNNCC 4 cut(s) 663, 889, 1006, 1078
NmuCI GTSAC 1 cut(s) 1109
OliI CACNNNNGTG 1 cut(s) 996
PdmI GAANNNNTTC 1 cut(s) 1033
PfeI GAWTC 3 cut(s) 165, 277, 922
PflMI CCANNNNNTGG 1 cut(s) 383
PfoI TCCNGGA 1 cut(s) 152
PkrI GCNGC 2 cut(s) 371, 666
PleI GAGTC 2 cut(s) 568, 1191
PpsI GAGTC 2 cut(s) 568, 1191
Ppu21I YACGTR 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 1177
Psp6I CCWGG 2 cut(s) 727, 762
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 2 cut(s) 727, 762
PspN4I GGNNCC 4 cut(s) 663, 889, 1006, 1078
PspOMI GGGCCC 1 cut(s) 887
PspPI GGNCC 3 cut(s) 102, 887, 888
PsuI RGATCY 2 cut(s) 262, 701
RsaI GTAC 3 cut(s) 409, 684, 860
RsaNI GTAC 3 cut(s) 408, 683, 859
RseI CAYNNNNRTG 1 cut(s) 996
SacI GAGCTC 1 cut(s) 1177
SaqAI TTAA 4 cut(s) 330, 779, 911, 1210
SatI GCNGC 2 cut(s) 370, 665
Sau3AI GATC 3 cut(s) 262, 649, 701
Sau96I GGNCC 3 cut(s) 102, 887, 888
SchI GAGTC 2 cut(s) 568, 1192
ScrFI CCNGG 3 cut(s) 154, 729, 764
SduI GDGCHC 3 cut(s) 114, 891, 1177
SfaNI GCATC 2 cut(s) 295, 760
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
SpeI ACTAGT 1 cut(s) 980
Sse9I AATT 7 cut(s) 35, 140, 158, 284, 509, 602, 714
SsiI CCGC 3 cut(s) 665, 1074, 1116
SspMI CTAG 1 cut(s) 981
SstI GAGCTC 1 cut(s) 1177
StyD4I CCNGG 3 cut(s) 152, 727, 762
StyI CCWWGG 2 cut(s) 578, 883
TaaI ACNGT 7 cut(s) 77, 109, 415, 493, 524, 632, 1023
TaiI ACGT 3 cut(s) 29, 688, 831
TaqI TCGA 3 cut(s) 23, 877, 1154
TasI AATT 7 cut(s) 35, 140, 158, 284, 509, 602, 714
TatI WGTACW 2 cut(s) 407, 858
TauI GCSGC 1 cut(s) 667
TfiI GAWTC 3 cut(s) 165, 277, 922
Tru1I TTAA 4 cut(s) 330, 779, 911, 1210
Tru9I TTAA 4 cut(s) 330, 779, 911, 1210
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 1 cut(s) 1109
TseI GCWGC 1 cut(s) 369
Tsp45I GTSAC 1 cut(s) 1109
TspDTI ATGAA 7 cut(s) 17, 115, 273, 410, 480, 625, 929
TspGWI ACGGA 1 cut(s) 1027
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 383
XapI RAATTY 1 cut(s) 158
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XmnI GAANNNNTTC 1 cut(s) 1033
XspI CTAG 1 cut(s) 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.