Rh2CG453000

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002322
Physical Location & Seq
Forward (+)
61566248 .. 61569730
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG453000.1

Sequence Viewer

Length: 1197 bp
ATGGTGAAAATGGTGGACTTGGGCCGATTCCACGCCCTGTTTCAAGGCTCGTCGGAGGAGTTTTGGCGGGAAAACGTTTATGGGGGATGGGCAACTCTTGTGGCTGTGCTTATTATCTTGGCTTGGCACTGTTTCAAGGGTTTTTTGTATAGGAAAACGTCTTCTTCCTCTGCTTCTTCATCTACAGTTGGTGTCTCTGATTCAAATCCCATTGCTTCTGGCTCTGGGGGTTCACAGACTAGAGTCTCGGAAATGGTAACAGATGCAGATTTGAAGTTTCTGATTGAAAATTTGGACGAGAAGATCGGTCAGAATAAGAAATGGGACAATGTCATAGAGAAAGGAAATGATCTTCTATACTACAGTGCTAAGTGCTGCAAGCCTAAGGTTGGTCCTGTGAAGTACTTGAGTGTGACAATATTTGAGGACTGCACACCTGAGATGCTTAGAGACTTCTACATGGACAATGAATACAGAAAGCAATGGGATAAAATGCTGATTGAGCATGAACAGTTACATGTGGACAAAAACAATGGGGTTGAAGTTGGCCGGACAATAAAGAAGTTTCCACTGTTGACACCTAGAGAGTATGTACTAGCTTGGAGACTGTGGGAGGGAAAAGATAAAACATTCTACTGTTTTGTCAAGGAATGTGAACATTCTTTGGCACCACGGCAGAAGAAGTATGTGCGTGTTAGTACTTTCAGATCTGGTTGGCGAATCAGAAAAGTACCTGGTAGAAATGCCTGCGAGATCAAAATGTATCACCAAGAAGATGCTGGTTTAAACGTGGAGATGGCAAAACTGGCCTTCGCGAAGGGCATATGGAGTTACGTATGTAAGATGGATAGTGCACTACGTAAATATTCTACAATCAGCAATGATCAATCAAGTTCTGCTGCCACTGCAGTCACTTTGATAAAAAAGGTCCCCCCTGGATTACAGGCCATGGATAGCAATGGAGCAACCTCTGCAGAACCTATTGTTCACAGACCAGTTACTGGTGGGGAGAGGAAATTGTCAAGAAGACCATCAAAAAAATTGCTAGCCAATGGCTTGCTGCTTGTTGGTGGTCTGGTCTGCCTATCTCGTGGTCGCTCAAGCCTGGGTGCAAAGGTTGCCATGGCATATATCTTGACCAAACTGAACAAGCGTGGTGCTTCATCAAGTCAAAATGGGGATATTGCAGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

398

Amino Acids

44.49

Weight (kDa)

9.46

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 139 - 259 2.4e-10 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 667
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccII CGCG 1 cut(s) 815
AciI CCGC 1 cut(s) 67
AclI AACGTT 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 547
AcsI RAATTY 1 cut(s) 289
AfaI GTAC 4 cut(s) 404, 594, 700, 732
AfiI CCNNNNNNNGG 2 cut(s) 389, 1001
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 6 cut(s) 44, 136, 204, 274, 287, 542
AjnI CCWGG 3 cut(s) 733, 934, 1104
AluBI AGCT 1 cut(s) 599
AluI AGCT 1 cut(s) 599
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 4 cut(s) 199, 250, 444, 598
Alw44I GTGCAC 1 cut(s) 852
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 4 cut(s) 22, 547, 807, 945
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 3 cut(s) 375, 899, 1060
ApoI RAATTY 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 22, 392, 928
AsuHPI GGTGA 2 cut(s) 16, 758
AsuNHI GCTAGC 1 cut(s) 1045
AvaII GGWCC 2 cut(s) 392, 928
AxyI CCTNAGG 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 856
BanI GGYRCC 1 cut(s) 667
BauI CACGAG 1 cut(s) 1089
BbsI GAAGAC 2 cut(s) 153, 1033
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 3 cut(s) 362, 886, 1047
BccI CCATC 4 cut(s) 81, 790, 838, 1039
BceAI ACGGC 1 cut(s) 689
BciT130I CCWGG 3 cut(s) 735, 936, 1106
BclI TGATCA 1 cut(s) 883
BcoDI GTCTC 4 cut(s) 199, 250, 444, 598
BfaI CTAG 4 cut(s) 240, 582, 596, 1046
BfmI CTRYAG 4 cut(s) 183, 361, 906, 972
BglII AGATCT 1 cut(s) 707
BisI GCNGC 3 cut(s) 376, 900, 1061
BlsI GCNGC 3 cut(s) 377, 901, 1062
BmcAI AGTACT 2 cut(s) 404, 700
Bme1390I CCNGG 3 cut(s) 735, 936, 1106
Bme18I GGWCC 2 cut(s) 392, 928
BmgT120I GGNCC 3 cut(s) 22, 392, 928
BmiI GGNNCC 2 cut(s) 669, 930
BmrFI CCNGG 3 cut(s) 735, 936, 1106
BmsI GCATC 3 cut(s) 253, 432, 766
BmtI GCTAGC 1 cut(s) 1049
BoxI GACNNNNGTC 1 cut(s) 242
BpiI GAAGAC 2 cut(s) 153, 1033
BpuEI CTTGAG 2 cut(s) 427, 1084
BsaAI YACGTR 2 cut(s) 835, 860
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 5 cut(s) 671, 934, 948, 1105, 1122
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 1001
Bse1I ACTGG 3 cut(s) 810, 995, 1006
Bse21I CCTNAGG 1 cut(s) 384
Bse3DI GCAATG 4 cut(s) 210, 488, 886, 964
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 3 cut(s) 735, 936, 1106
BseDI CCNNGG 5 cut(s) 671, 934, 948, 1105, 1122
BseGI GGATG 1 cut(s) 92
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 2 cut(s) 389, 1001
BseMI GCAATG 4 cut(s) 210, 488, 886, 964
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 3 cut(s) 810, 995, 1006
BseRI GAGGAG 1 cut(s) 71
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 3 cut(s) 362, 886, 1047
BsgI GTGCAG 1 cut(s) 415
Bsh1236I CGCG 1 cut(s) 815
BshFI GGCC 4 cut(s) 24, 549, 809, 947
BshNI GGYRCC 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 856
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 2 cut(s) 338, 914
BslI CCNNNNNNNGG 2 cut(s) 389, 1001
BsmAI GTCTC 4 cut(s) 199, 250, 444, 598
BsmFI GGGAC 2 cut(s) 338, 914
BsnI GGCC 4 cut(s) 24, 549, 809, 947
Bsp1286I GDGCHC 1 cut(s) 856
Bsp143I GATC 5 cut(s) 303, 349, 707, 753, 883
Bsp19I CCATGG 2 cut(s) 948, 1122
Bsp68I TCGCGA 1 cut(s) 815
BspACI CCGC 1 cut(s) 67
BspANI GGCC 4 cut(s) 24, 549, 809, 947
BspCNI CTCAG 1 cut(s) 430
BspFNI CGCG 1 cut(s) 815
BspLI GGNNCC 2 cut(s) 669, 930
BspMAI CTGCAG 2 cut(s) 910, 976
BspOI GCTAGC 1 cut(s) 1049
BspT107I GGYRCC 1 cut(s) 667
BsrDI GCAATG 4 cut(s) 210, 488, 886, 964
BsrI ACTGG 3 cut(s) 810, 995, 1006
BssECI CCNNGG 5 cut(s) 671, 934, 948, 1105, 1122
BssMI GATC 5 cut(s) 303, 349, 707, 753, 883
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 2 cut(s) 948, 1122
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 3 cut(s) 735, 936, 1106
Bst4CI ACNGT 7 cut(s) 131, 187, 365, 513, 573, 609, 638
BstAPI GCANNNNNTGC 2 cut(s) 971, 1118
BstBAI YACGTR 2 cut(s) 835, 860
BstC8I GCNNGC 5 cut(s) 380, 748, 1047, 1058, 1189
BstDEI CTNAG 4 cut(s) 369, 384, 438, 446
BstDSI CCRYGG 3 cut(s) 671, 948, 1122
BstF5I GGATG 1 cut(s) 92
BstFNI CGCG 1 cut(s) 815
BstKTI GATC 5 cut(s) 306, 352, 710, 756, 886
BstMAI GTCTC 4 cut(s) 199, 250, 444, 598
BstMBI GATC 5 cut(s) 303, 349, 707, 753, 883
BstMWI GCNNNNNNNGC 5 cut(s) 502, 806, 905, 971, 1118
BstNI CCWGG 3 cut(s) 735, 936, 1106
BstNSI RCATGY 1 cut(s) 521
BstPAI GACNNNNGTC 1 cut(s) 242
BstSCI CCNGG 3 cut(s) 733, 934, 1104
BstSFI CTRYAG 4 cut(s) 183, 361, 906, 972
BstSLI GKGCMC 1 cut(s) 856
BstSNI TACGTA 2 cut(s) 835, 860
BstUI CGCG 1 cut(s) 815
BstV1I GCAGC 3 cut(s) 362, 886, 1047
BstV2I GAAGAC 2 cut(s) 153, 1033
BstX2I RGATCY 1 cut(s) 707
BstYI RGATCY 1 cut(s) 707
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 4 cut(s) 24, 549, 809, 947
BtgI CCRYGG 3 cut(s) 671, 948, 1122
BtsCI GGATG 1 cut(s) 92
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 4 cut(s) 127, 370, 569, 903
BtuMI TCGCGA 1 cut(s) 815
Cac8I GCNNGC 5 cut(s) 380, 748, 1047, 1058, 1189
CaiI CAGNNNCTG 1 cut(s) 1001
Cfr13I GGNCC 3 cut(s) 22, 392, 928
CsiI ACCWGGT 1 cut(s) 733
Csp6I GTAC 4 cut(s) 403, 593, 699, 731
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviAII CATG 5 cut(s) 460, 506, 518, 949, 1123
CviQI GTAC 4 cut(s) 403, 593, 699, 731
DdeI CTNAG 4 cut(s) 369, 384, 438, 446
DpnI GATC 5 cut(s) 305, 351, 709, 755, 885
DpnII GATC 5 cut(s) 303, 349, 707, 753, 883
DraI TTTAAA 1 cut(s) 786
EaeI YGGCCR 1 cut(s) 547
Eco105I TACGTA 2 cut(s) 835, 860
Eco130I CCWWGG 2 cut(s) 948, 1122
Eco47I GGWCC 2 cut(s) 392, 928
Eco81I CCTNAGG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 928
EcoRII CCWGG 3 cut(s) 733, 934, 1104
EcoT14I CCWWGG 2 cut(s) 948, 1122
ErhI CCWWGG 2 cut(s) 948, 1122
FaeI CATG 5 cut(s) 463, 509, 521, 952, 1126
FaqI GGGAC 2 cut(s) 338, 914
FatI CATG 5 cut(s) 459, 505, 517, 948, 1122
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 2 cut(s) 824, 1193
FbaI TGATCA 1 cut(s) 883
Fnu4HI GCNGC 3 cut(s) 376, 900, 1061
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 376, 900, 1061
FspBI CTAG 4 cut(s) 240, 582, 596, 1046
GluI GCNGC 3 cut(s) 376, 900, 1061
HaeIII GGCC 4 cut(s) 24, 549, 809, 947
HapII CCGG 1 cut(s) 550
Hin1II CATG 5 cut(s) 463, 509, 521, 952, 1126
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HinfI GANTC 4 cut(s) 27, 200, 243, 720
HpaII CCGG 1 cut(s) 550
HphI GGTGA 2 cut(s) 16, 758
Hpy166II GTNNAC 7 cut(s) 16, 233, 523, 576, 656, 854, 988
Hpy188I TCNGA 7 cut(s) 55, 199, 250, 282, 312, 707, 725
Hpy188III TCNNGA 3 cut(s) 814, 1023, 1135
Hpy8I GTNNAC 7 cut(s) 16, 233, 523, 576, 656, 854, 988
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 2 cut(s) 811, 820
HpyCH4III ACNGT 7 cut(s) 131, 187, 365, 513, 573, 609, 638
HpyCH4IV ACGT 5 cut(s) 75, 158, 789, 834, 859
HpyCH4V TGCA 8 cut(s) 266, 378, 432, 854, 908, 974, 1112, 1187
HpyF10VI GCNNNNNNNGC 5 cut(s) 502, 806, 905, 971, 1118
HpyF3I CTNAG 4 cut(s) 369, 384, 438, 446
HpySE526I ACGT 5 cut(s) 75, 158, 789, 834, 859
Hsp92II CATG 5 cut(s) 463, 509, 521, 952, 1126
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 5 cut(s) 303, 349, 707, 753, 883
LmnI GCTCC 1 cut(s) 962
Lsp1109I GCAGC 3 cut(s) 362, 886, 1047
LweI GCATC 3 cut(s) 253, 432, 766
MabI ACCWGGT 1 cut(s) 733
MaeI CTAG 4 cut(s) 240, 582, 596, 1046
MaeII ACGT 5 cut(s) 75, 158, 789, 834, 859
MaeIII GTNAC 6 cut(s) 256, 412, 513, 830, 910, 997
MalI GATC 5 cut(s) 305, 351, 709, 755, 885
MboI GATC 5 cut(s) 303, 349, 707, 753, 883
MboII GAAGA 8 cut(s) 153, 156, 168, 313, 344, 691, 785, 1038
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 1 cut(s) 856
MluCI AATT 3 cut(s) 289, 1016, 1040
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 6 cut(s) 49, 178, 418, 607, 979, 1005
MseI TTAA 1 cut(s) 785
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 3 cut(s) 735, 936, 1106
MssI GTTTAAAC 1 cut(s) 786
MvaI CCWGG 3 cut(s) 735, 936, 1106
MvnI CGCG 1 cut(s) 815
MwoI GCNNNNNNNGC 5 cut(s) 502, 806, 905, 971, 1118
NcoI CCATGG 2 cut(s) 948, 1122
NdeI CATATG 2 cut(s) 824, 1193
NdeII GATC 5 cut(s) 303, 349, 707, 753, 883
NheI GCTAGC 1 cut(s) 1045
NlaIII CATG 5 cut(s) 463, 509, 521, 952, 1126
NlaIV GGNNCC 2 cut(s) 669, 930
NmuCI GTSAC 2 cut(s) 412, 910
NruI TCGCGA 1 cut(s) 815
NspI RCATGY 1 cut(s) 521
PciI ACATGT 1 cut(s) 517
PfeI GAWTC 3 cut(s) 27, 200, 720
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 3 cut(s) 377, 901, 1062
PleI GAGTC 1 cut(s) 251
PmeI GTTTAAAC 1 cut(s) 786
PpsI GAGTC 1 cut(s) 251
Ppu21I YACGTR 2 cut(s) 835, 860
PpuMI RGGWCCY 1 cut(s) 928
PscI ACATGT 1 cut(s) 517
PshAI GACNNNNGTC 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 928
Psp6I CCWGG 3 cut(s) 733, 934, 1104
PspGI CCWGG 3 cut(s) 733, 934, 1104
PspN4I GGNNCC 2 cut(s) 669, 930
PspPI GGNCC 3 cut(s) 22, 392, 928
PspPPI RGGWCCY 1 cut(s) 928
PstI CTGCAG 2 cut(s) 910, 976
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 707
PsyI GACNNNGTC 1 cut(s) 329
RruI TCGCGA 1 cut(s) 815
RsaI GTAC 4 cut(s) 404, 594, 700, 732
RsaNI GTAC 4 cut(s) 403, 593, 699, 731
SaqAI TTAA 1 cut(s) 785
SatI GCNGC 3 cut(s) 376, 900, 1061
Sau3AI GATC 5 cut(s) 303, 349, 707, 753, 883
Sau96I GGNCC 3 cut(s) 22, 392, 928
ScaI AGTACT 2 cut(s) 404, 700
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 3 cut(s) 735, 936, 1106
SduI GDGCHC 1 cut(s) 856
SexAI ACCWGGT 1 cut(s) 733
SfaNI GCATC 3 cut(s) 253, 432, 766
SfcI CTRYAG 4 cut(s) 183, 361, 906, 972
SinI GGWCC 2 cut(s) 392, 928
SmlI CTYRAG 2 cut(s) 406, 1099
SmoI CTYRAG 2 cut(s) 406, 1099
SnaBI TACGTA 2 cut(s) 835, 860
Sse9I AATT 3 cut(s) 289, 1016, 1040
SsiI CCGC 1 cut(s) 67
SspI AATATT 2 cut(s) 420, 866
SspMI CTAG 4 cut(s) 240, 582, 596, 1046
StyD4I CCNGG 3 cut(s) 733, 934, 1104
StyI CCWWGG 2 cut(s) 948, 1122
TaaI ACNGT 7 cut(s) 131, 187, 365, 513, 573, 609, 638
TaiI ACGT 5 cut(s) 78, 161, 792, 837, 862
TaqII GACCGA 1 cut(s) 296
TasI AATT 3 cut(s) 289, 1016, 1040
TatI WGTACW 3 cut(s) 402, 592, 698
TfiI GAWTC 3 cut(s) 27, 200, 720
Tru1I TTAA 1 cut(s) 785
Tru9I TTAA 1 cut(s) 785
TscAI CASTG 4 cut(s) 134, 370, 576, 910
TseFI GTSAC 2 cut(s) 412, 910
TseI GCWGC 3 cut(s) 375, 899, 1060
Tsp45I GTSAC 2 cut(s) 412, 910
TspDTI ATGAA 4 cut(s) 168, 483, 522, 1152
TspRI CASTG 4 cut(s) 134, 370, 576, 910
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 1001
VneI GTGCAC 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 392, 928
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 521
XcmI CCANNNNNNNNNTGG 1 cut(s) 776
XspI CTAG 4 cut(s) 240, 582, 596, 1046
ZrmI AGTACT 2 cut(s) 404, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.