MD17G1158100.v1.1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002322
Physical Location & Seq
Forward (+)
15295583 .. 15306020
10438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1158100.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGTCAAAATCGCGGATTCGAGCAGATTTCACGGCCTATTCGTACGGTCGTCGGAGGAATTTTGGCGCGAACATATCCATGGCGGTTTCGCCACTCTCGTCGCCGTGCTCTTCGTCTTTGCTTGCCACTGTGCCAAGAGGTTCTTCTTTGGAAAACCCTCTGGCTCCAGTGCTTCTCCGGTCGGTCTTTCGGATTCTGATCTCCCCACTTCTGGGTCTCGGATTCCGCCGCACAGCACCCTGGAACTGGTGACAGATGCAGATTTGAAGTTTCTGATTGATAACTTGGAGGAGAAAATGGGCGAAAATGAGAAATGGGAAAAGGTCATAGACAAAAGAAATGATATTCTATCCTACTATGCGAAGTGCTGCAAGCCTAAGGATAGTTCTTTGACATACTTGAGCGTGACGATATTTGAGAATTGCTCTCCTGAGAAGCTGAGAGACTTCTACATGGACAATGATTACAGGAAGCAATGGGATAAGATGCTGATTCAGCATGAACAGTTGCAGATGGACAAAAACAAGGGGGTTGAAGTTGCTTGGAGATTGTGGGAGGGGAAAGATAACACCTTTTATTGTTATATCAAGGAATGTGAACATCCTTTGGCACCACCCCAGAAGAAGTATGTGCGTGTTAGTACTTTCAGATCTGGTTGGCGAATCCAAAAAGTGCCTGATACTAATGCCTGTGAGATCAAGATGTTTCATCAAGAAGATGCTGGTTTAAATGTGGAGATGGCAAAATTGGCTTTTTCTAGGGGCATATGGAGCTATGTGTGCAAGATGGGCAGTGCACTTAACAGATACGCTACAATCAGCTCTCATCAATCAAGGGCTGCTGCTTCTGCTGTCACGTTGATTAAAAAGGTCCCCCCTGGATTAGAAGCCACGGATGACATGACCTCTCAAGCAACCTCAGTAGGAACTTCTGTTCGCAGACCAATTGCGGGCGAGGGAAGGAAATTGTCAATAAGACCTTCAAAGAAATTGTTAGCCAATGGCCTGCTGCTTCTTGGGGGAGTGGTCTTCCTGTCTCGTGGTCACTCTAGCCTGGGCGCGAAGGTTGCCATGGCTTACATCTTAACGAAGTGGAGTAAGCGTGGCGCTTCATCAAGTCAAAGCAGTGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

379

Amino Acids

42.42

Weight (kDa)

9.17

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 610
AccII CGCG 3 cut(s) 14, 69, 1061
AciI CCGC 5 cut(s) 14, 84, 227, 230, 950
AcsI RAATTY 2 cut(s) 59, 1131
AfaI GTAC 2 cut(s) 45, 643
AfiI CCNNNNNNNGG 4 cut(s) 212, 213, 247, 950
AgsI TTSAA 3 cut(s) 268, 536, 984
AjnI CCWGG 3 cut(s) 240, 877, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AloI GAACNNNNNNTCC 2 cut(s) 918, 950
AluBI AGCT 3 cut(s) 439, 774, 822
AluI AGCT 3 cut(s) 439, 774, 822
Alw21I GWGCWC 2 cut(s) 111, 799
Alw26I GTCTC 3 cut(s) 222, 438, 1041
Alw44I GTGCAC 1 cut(s) 795
AoxI GGCC 2 cut(s) 34, 1003
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 4 cut(s) 369, 839, 842, 1009
ApoI RAATTY 2 cut(s) 59, 1131
AspLEI GCGC 3 cut(s) 69, 1061, 1109
AspS9I GGNCC 1 cut(s) 871
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 871
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 799
BanI GGYRCC 1 cut(s) 610
BauI CACGAG 1 cut(s) 1038
BbsI GAAGAC 1 cut(s) 1021
Bbv12I GWGCWC 2 cut(s) 111, 799
BbvI GCAGC 4 cut(s) 356, 826, 829, 996
BccI CCATC 3 cut(s) 508, 733, 781
BceAI ACGGC 2 cut(s) 49, 89
BciT130I CCWGG 3 cut(s) 242, 879, 1055
BcoDI GTCTC 3 cut(s) 222, 438, 1041
BfaI CTAG 2 cut(s) 759, 1050
BfoI RGCGCY 1 cut(s) 1110
BglII AGATCT 1 cut(s) 650
BisI GCNGC 5 cut(s) 230, 370, 840, 843, 1010
BlsI GCNGC 5 cut(s) 231, 371, 841, 844, 1011
BmcAI AGTACT 1 cut(s) 643
Bme1390I CCNGG 3 cut(s) 242, 879, 1055
Bme18I GGWCC 1 cut(s) 871
BmgT120I GGNCC 1 cut(s) 871
BmiI GGNNCC 3 cut(s) 166, 612, 873
BmrFI CCNGG 3 cut(s) 242, 879, 1055
BmsI GCATC 3 cut(s) 247, 477, 709
BpiI GAAGAC 1 cut(s) 1021
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpmI CTGGAG 1 cut(s) 151
BpuEI CTTGAG 2 cut(s) 421, 894
BsaBI GATNNNNATC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 6 cut(s) 79, 240, 877, 891, 1054, 1071
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 213, 247, 950
Bse1I ACTGG 2 cut(s) 168, 252
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 198
BseBI CCWGG 3 cut(s) 242, 879, 1055
BseDI CCNNGG 6 cut(s) 79, 240, 877, 891, 1054, 1071
BseGI GGATG 2 cut(s) 601, 901
BseJI GATNNNNATC 1 cut(s) 198
BseLI CCNNNNNNNGG 4 cut(s) 212, 213, 247, 950
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 3 cut(s) 423, 431, 933
BseNI ACTGG 2 cut(s) 168, 252
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 4 cut(s) 356, 826, 829, 996
Bsh1236I CGCG 3 cut(s) 14, 69, 1061
Bsh1285I CGRYCG 2 cut(s) 50, 183
BshFI GGCC 2 cut(s) 36, 1005
BshNI GGYRCC 1 cut(s) 610
BsiEI CGRYCG 2 cut(s) 50, 183
BsiHKAI GWGCWC 2 cut(s) 111, 799
BsiSI CCGG 1 cut(s) 179
BsiWI CGTACG 1 cut(s) 43
BslFI GGGAC 1 cut(s) 857
BslI CCNNNNNNNGG 4 cut(s) 212, 213, 247, 950
BsmAI GTCTC 3 cut(s) 222, 438, 1041
BsmFI GGGAC 1 cut(s) 857
BsnI GGCC 2 cut(s) 36, 1005
Bso31I GGTCTC 1 cut(s) 222
Bsp1286I GDGCHC 2 cut(s) 111, 799
Bsp143I GATC 3 cut(s) 199, 650, 696
Bsp19I CCATGG 2 cut(s) 79, 1071
BspACI CCGC 5 cut(s) 14, 84, 227, 230, 950
BspANI GGCC 2 cut(s) 36, 1005
BspCNI CTCAG 3 cut(s) 424, 432, 932
BspFNI CGCG 3 cut(s) 14, 69, 1061
BspLI GGNNCC 3 cut(s) 166, 612, 873
BspQI GCTCTTC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 610
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 482
BsrI ACTGG 2 cut(s) 168, 252
BssECI CCNNGG 6 cut(s) 79, 240, 877, 891, 1054, 1071
BssMI GATC 3 cut(s) 199, 650, 696
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 2 cut(s) 79, 1071
Bst2BI CACGAG 1 cut(s) 1038
Bst2UI CCWGG 3 cut(s) 242, 879, 1055
Bst4CI ACNGT 3 cut(s) 48, 131, 507
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 4 cut(s) 124, 374, 952, 1007
BstDEI CTNAG 4 cut(s) 378, 432, 440, 919
BstDSI CCRYGG 3 cut(s) 79, 891, 1071
BstF5I GGATG 2 cut(s) 601, 901
BstFNI CGCG 3 cut(s) 14, 69, 1061
BstH2I RGCGCY 1 cut(s) 1110
BstHHI GCGC 3 cut(s) 69, 1061, 1109
BstKTI GATC 3 cut(s) 202, 653, 699
BstMAI GTCTC 3 cut(s) 222, 438, 1041
BstMBI GATC 3 cut(s) 199, 650, 696
BstMCI CGRYCG 2 cut(s) 50, 183
BstMWI GCNNNNNNNGC 7 cut(s) 496, 749, 771, 780, 789, 848, 1067
BstNI CCWGG 3 cut(s) 242, 879, 1055
BstSCI CCNGG 3 cut(s) 240, 877, 1053
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 3 cut(s) 14, 69, 1061
BstV1I GCAGC 4 cut(s) 356, 826, 829, 996
BstV2I GAAGAC 1 cut(s) 1021
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 2 cut(s) 36, 1005
BtgI CCRYGG 3 cut(s) 79, 891, 1071
BtsCI GGATG 2 cut(s) 601, 901
BtsI GCAGTG 2 cut(s) 799, 1132
BtsIMutI CAGTG 4 cut(s) 127, 175, 799, 1132
Cac8I GCNNGC 4 cut(s) 124, 374, 952, 1007
CfoI GCGC 3 cut(s) 69, 1061, 1109
Cfr13I GGNCC 1 cut(s) 871
Csp6I GTAC 2 cut(s) 44, 642
CviAII CATG 5 cut(s) 80, 454, 500, 901, 1072
CviQI GTAC 2 cut(s) 44, 642
DdeI CTNAG 4 cut(s) 378, 432, 440, 919
DpnI GATC 3 cut(s) 201, 652, 698
DpnII GATC 3 cut(s) 199, 650, 696
DraI TTTAAA 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 79, 1071
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 871
Eco81I CCTNAGG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 871
EcoRII CCWGG 3 cut(s) 240, 877, 1053
EcoT14I CCWWGG 2 cut(s) 79, 1071
ErhI CCWWGG 2 cut(s) 79, 1071
FaeI CATG 5 cut(s) 83, 457, 503, 904, 1075
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 1 cut(s) 857
FatI CATG 5 cut(s) 79, 453, 499, 900, 1071
FauI CCCGC 1 cut(s) 943
FauNDI CATATG 1 cut(s) 767
Fnu4HI GCNGC 5 cut(s) 230, 370, 840, 843, 1010
FokI GGATG 2 cut(s) 588, 908
Fsp4HI GCNGC 5 cut(s) 230, 370, 840, 843, 1010
FspBI CTAG 2 cut(s) 759, 1050
GlaI GCGC 3 cut(s) 68, 1060, 1108
GluI GCNGC 5 cut(s) 230, 370, 840, 843, 1010
GsuI CTGGAG 1 cut(s) 151
HaeII RGCGCY 1 cut(s) 1110
HaeIII GGCC 2 cut(s) 36, 1005
HapII CCGG 1 cut(s) 179
HhaI GCGC 3 cut(s) 69, 1061, 1109
Hin1II CATG 5 cut(s) 83, 457, 503, 904, 1075
Hin6I GCGC 3 cut(s) 67, 1059, 1107
HinP1I GCGC 3 cut(s) 67, 1059, 1107
HinfI GANTC 5 cut(s) 17, 194, 223, 493, 663
HpaII CCGG 1 cut(s) 179
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 2 cut(s) 599, 797
Hpy188I TCNGA 6 cut(s) 55, 193, 199, 222, 276, 650
Hpy188III TCNNGA 3 cut(s) 431, 700, 713
Hpy8I GTNNAC 2 cut(s) 599, 797
Hpy99I CGWCG 2 cut(s) 55, 104
HpyAV CCTTC 3 cut(s) 954, 990, 1057
HpyCH4III ACNGT 3 cut(s) 48, 131, 507
HpyCH4IV ACGT 1 cut(s) 857
HpyCH4V TGCA 5 cut(s) 260, 372, 511, 783, 797
HpyF10VI GCNNNNNNNGC 7 cut(s) 496, 749, 771, 780, 789, 848, 1067
HpyF3I CTNAG 4 cut(s) 378, 432, 440, 919
HpySE526I ACGT 1 cut(s) 857
Hsp92II CATG 5 cut(s) 83, 457, 503, 904, 1075
HspAI GCGC 3 cut(s) 67, 1059, 1107
Kzo9I GATC 3 cut(s) 199, 650, 696
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 2 cut(s) 170, 771
Lsp1109I GCAGC 4 cut(s) 356, 826, 829, 996
LweI GCATC 3 cut(s) 247, 477, 709
MaeI CTAG 2 cut(s) 759, 1050
MaeII ACGT 1 cut(s) 857
MaeIII GTNAC 4 cut(s) 250, 406, 853, 1043
MalI GATC 3 cut(s) 201, 652, 698
MboI GATC 3 cut(s) 199, 650, 696
MboII GAAGA 5 cut(s) 103, 136, 634, 728, 1021
MfeI CAATTG 1 cut(s) 945
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 111, 799
MluCI AATT 7 cut(s) 59, 421, 746, 945, 965, 989, 1131
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 8 cut(s) 49, 132, 169, 283, 550, 916, 928, 949
MseI TTAA 4 cut(s) 728, 801, 864, 1085
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 3 cut(s) 242, 879, 1055
MunI CAATTG 1 cut(s) 945
MvaI CCWGG 3 cut(s) 242, 879, 1055
MvnI CGCG 3 cut(s) 14, 69, 1061
MwoI GCNNNNNNNGC 7 cut(s) 496, 749, 771, 780, 789, 848, 1067
NcoI CCATGG 2 cut(s) 79, 1071
NdeI CATATG 1 cut(s) 767
NdeII GATC 3 cut(s) 199, 650, 696
NlaIII CATG 5 cut(s) 83, 457, 503, 904, 1075
NlaIV GGNNCC 3 cut(s) 166, 612, 873
NmuCI GTSAC 4 cut(s) 250, 406, 853, 1043
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 96
PfeI GAWTC 5 cut(s) 17, 194, 223, 493, 663
Pfl23II CGTACG 1 cut(s) 43
PkrI GCNGC 5 cut(s) 231, 371, 841, 844, 1011
PpuMI RGGWCCY 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 3 cut(s) 240, 877, 1053
PspGI CCWGG 3 cut(s) 240, 877, 1053
PspLI CGTACG 1 cut(s) 43
PspN4I GGNNCC 3 cut(s) 166, 612, 873
PspPI GGNCC 1 cut(s) 871
PspPPI RGGWCCY 1 cut(s) 871
PsuI RGATCY 1 cut(s) 650
RsaI GTAC 2 cut(s) 45, 643
RsaNI GTAC 2 cut(s) 44, 642
RseI CAYNNNNRTG 1 cut(s) 78
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 4 cut(s) 728, 801, 864, 1085
SatI GCNGC 5 cut(s) 230, 370, 840, 843, 1010
Sau3AI GATC 3 cut(s) 199, 650, 696
Sau96I GGNCC 1 cut(s) 871
ScaI AGTACT 1 cut(s) 643
ScrFI CCNGG 3 cut(s) 242, 879, 1055
SduI GDGCHC 2 cut(s) 111, 799
SfaNI GCATC 3 cut(s) 247, 477, 709
SinI GGWCC 1 cut(s) 871
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 2 cut(s) 400, 909
SmoI CTYRAG 2 cut(s) 400, 909
Sse9I AATT 7 cut(s) 59, 421, 746, 945, 965, 989, 1131
SsiI CCGC 5 cut(s) 14, 84, 227, 230, 950
SspMI CTAG 2 cut(s) 759, 1050
StyD4I CCNGG 3 cut(s) 240, 877, 1053
StyI CCWWGG 2 cut(s) 79, 1071
TaaI ACNGT 3 cut(s) 48, 131, 507
TaiI ACGT 1 cut(s) 860
TaqI TCGA 1 cut(s) 20
TaqII GACCGA 1 cut(s) 173
TasI AATT 7 cut(s) 59, 421, 746, 945, 965, 989, 1131
TatI WGTACW 1 cut(s) 641
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 17, 194, 223, 493, 663
Tru1I TTAA 4 cut(s) 728, 801, 864, 1085
Tru9I TTAA 4 cut(s) 728, 801, 864, 1085
TscAI CASTG 4 cut(s) 134, 175, 799, 1132
TseFI GTSAC 4 cut(s) 250, 406, 853, 1043
TseI GCWGC 4 cut(s) 369, 839, 842, 1009
Tsp45I GTSAC 4 cut(s) 250, 406, 853, 1043
TspDTI ATGAA 3 cut(s) 516, 698, 1101
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 4 cut(s) 134, 175, 799, 1132
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 871
XapI RAATTY 2 cut(s) 59, 1131
XspI CTAG 2 cut(s) 759, 1050
ZrmI AGTACT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.