MD09G1168600.v1.1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002322
Physical Location & Seq
Reverse (-)
13960876 .. 13964966
4091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1168600.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGTGAAATTCGCGGATTCCGGTAGATTTCACGGCCTATTCCTTGGTTCGTCGGAGGAATTTTGGCGCGAAAACATTCATGGCGGTTTCGCCACTCTCGTCGCTGTGCTCTTCGTCTTGGCTTGCCACTGTGCCAAGAGGTTTTTCTTTCGGAAATCCTCTGCCTCCAGTGCTTCTCCGGTTCTATCGGATTCTGATCCCCCCACTTCTGGCTCTCGAAGTCCGCAGCTCAGAACCTCAGAACCAGTGACAGATGCAGATTTGAAGTTTCTGATTGATAACTTGGAGGAGAAGACAGGCCAAAATGTGAAATGGGAAAATGTCATAGACAAAAGAAATGATATTCTATCTTACTATGCTAAGTGCTGCAAGCCTAAGGATGGTCCTTTGACATACTTGAGTGTGACGATATTTGAGAATTGCTCTCCTGAGAAGCTGAGAGACTTCTACATGGACAATGATTACAGAAAACAATGGGACAAGATGCTGATTCAGCATGAGCAGTTACAGATGGACAAAAACAATGGGGTTGAAGTTGGTCGGACAATAAAAAAGTTTCCACTGTTGACAGCTAGAGAATATGTATTAGCTTGGAGATTGTGGGAGGGAAAAGATAAAACCTTTTATTGTTTTATCAAGGAATGTGAACATCCTTTGGCACCACGCCAGAAGAAGTATGTGCGTGTTCGTACCTTCAGATCTGGTTGGCGAATCCGAAAAGTGCCTAGTACTAATGCTTGTGAGATCAAGATGTTTCACCAAGAAGATGCTGGTTTAAATGTGGAGATGGCAAAATTGGCCTTTTCTAGGGGCATATGGAGCTATGTGTGCAAGATGGGCAGCGCACTTAACAGATATTCGACAATCAGCTCTCATCAATCAAGTTCTGCTGCTTCTGCTGTCACGTTAATTAAAAGGGTTCCCCCTGGATTAGAAGCCACGGATGAAATGACCTCTCAAGCAACCTCAGTAGCAACGTCTGTTTGCAGACCAACCACGGGCGAGGGAAGGAAATTGTCAAGAACACCTTCAACGAAATTGTTGGCCAATGGCCTGCTGCTTCTTGGGGGAGTGGTCTGCCTGTCTCGTGGTCACTCTAGCCTGGGCGCTAAGGTTGCCATGGCTTACATCTTAACGAAGTGGAGTAAGCGTGGCGCTTCATCAAGTCAAAGAAGTGAAATTTCTGGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

398

Amino Acids

44.51

Weight (kDa)

9.48

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 98 - 253 5.2e-09 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 658
AccII CGCG 2 cut(s) 14, 69
AciI CCGC 3 cut(s) 14, 84, 224
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 3 cut(s) 8, 59, 1179
AcuI CTGAAG 1 cut(s) 679
AfaI GTAC 2 cut(s) 691, 730
AfiI CCNNNNNNNGG 4 cut(s) 209, 380, 807, 998
AgsI TTSAA 3 cut(s) 265, 533, 1032
AjnI CCWGG 2 cut(s) 925, 1101
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 6 cut(s) 229, 436, 572, 590, 822, 870
AluI AGCT 6 cut(s) 229, 436, 572, 590, 822, 870
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 2 cut(s) 435, 1089
AlwI GGATC 1 cut(s) 191
AoxI GGCC 5 cut(s) 34, 298, 798, 1044, 1051
ApeKI GCWGC 5 cut(s) 226, 366, 840, 890, 1057
ApoI RAATTY 3 cut(s) 8, 59, 1179
Asp700I GAANNNNTTC 2 cut(s) 75, 1027
AspLEI GCGC 4 cut(s) 69, 845, 1109, 1157
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 2 cut(s) 16, 749
AvaII GGWCC 1 cut(s) 383
AxyI CCTNAGG 1 cut(s) 375
BalI TGGCCA 1 cut(s) 1046
BanI GGYRCC 1 cut(s) 658
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 5 cut(s) 238, 353, 852, 877, 1044
BccI CCATC 4 cut(s) 374, 505, 781, 829
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 2 cut(s) 927, 1103
BcoDI GTCTC 2 cut(s) 435, 1089
BfaI CTAG 4 cut(s) 573, 726, 807, 1098
BfoI RGCGCY 2 cut(s) 1110, 1158
BglII AGATCT 1 cut(s) 698
BisI GCNGC 5 cut(s) 227, 367, 841, 891, 1058
BlsI GCNGC 5 cut(s) 228, 368, 842, 892, 1059
BmcAI AGTACT 1 cut(s) 730
Bme1390I CCNGG 2 cut(s) 927, 1103
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 2 cut(s) 660, 921
BmrFI CCNGG 2 cut(s) 927, 1103
BmsI GCATC 3 cut(s) 244, 474, 757
BpiI GAAGAC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 1 cut(s) 151
Bpu10I CCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 418, 942
BsaBI GATNNNNATC 1 cut(s) 195
BsaJI CCNNGG 6 cut(s) 43, 925, 939, 996, 1102, 1119
BsaWI WCCGGW 2 cut(s) 20, 178
BsaXI ACNNNNNCTCC 2 cut(s) 1135, 1165
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 380, 807, 998
Bse1I ACTGG 2 cut(s) 168, 245
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 2 cut(s) 927, 1103
BseDI CCNNGG 6 cut(s) 43, 925, 939, 996, 1102, 1119
BseGI GGATG 3 cut(s) 385, 649, 949
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 4 cut(s) 209, 380, 807, 998
BseMII CTCAG 5 cut(s) 244, 252, 420, 428, 981
BseNI ACTGG 2 cut(s) 168, 245
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 5 cut(s) 238, 353, 852, 877, 1044
Bsh1236I CGCG 2 cut(s) 14, 69
BshFI GGCC 5 cut(s) 36, 300, 800, 1046, 1053
BshNI GGYRCC 1 cut(s) 658
BsiHKAI GWGCWC 1 cut(s) 111
BsiSI CCGG 2 cut(s) 21, 179
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 4 cut(s) 209, 380, 807, 998
BsmAI GTCTC 2 cut(s) 435, 1089
BsmFI GGGAC 1 cut(s) 491
BsnI GGCC 5 cut(s) 36, 300, 800, 1046, 1053
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 3 cut(s) 196, 698, 744
Bsp19I CCATGG 1 cut(s) 1119
BspACI CCGC 3 cut(s) 14, 84, 224
BspANI GGCC 5 cut(s) 36, 300, 800, 1046, 1053
BspCNI CTCAG 5 cut(s) 243, 251, 421, 429, 980
BspFNI CGCG 2 cut(s) 14, 69
BspLI GGNNCC 2 cut(s) 660, 921
BspPI GGATC 1 cut(s) 191
BspQI GCTCTTC 1 cut(s) 116
BspT107I GGYRCC 1 cut(s) 658
BsrI ACTGG 2 cut(s) 168, 245
BssECI CCNNGG 6 cut(s) 43, 925, 939, 996, 1102, 1119
BssMI GATC 3 cut(s) 196, 698, 744
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 2 cut(s) 43, 1119
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 2 cut(s) 927, 1103
Bst4CI ACNGT 2 cut(s) 131, 564
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 3 cut(s) 124, 371, 1055
BstDEI CTNAG 8 cut(s) 230, 238, 360, 375, 429, 437, 967, 1110
BstDSI CCRYGG 3 cut(s) 939, 996, 1119
BstENI CCTNNNNNAGG 1 cut(s) 805
BstF5I GGATG 3 cut(s) 385, 649, 949
BstFNI CGCG 2 cut(s) 14, 69
BstH2I RGCGCY 2 cut(s) 1110, 1158
BstHHI GCGC 4 cut(s) 69, 845, 1109, 1157
BstKTI GATC 3 cut(s) 199, 701, 747
BstMAI GTCTC 2 cut(s) 435, 1089
BstMBI GATC 3 cut(s) 196, 698, 744
BstMWI GCNNNNNNNGC 8 cut(s) 170, 493, 797, 819, 828, 837, 896, 1115
BstNI CCWGG 2 cut(s) 927, 1103
BstSCI CCNGG 2 cut(s) 925, 1101
BstUI CGCG 2 cut(s) 14, 69
BstV1I GCAGC 5 cut(s) 238, 353, 852, 877, 1044
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 5 cut(s) 36, 300, 800, 1046, 1053
BtgI CCRYGG 3 cut(s) 939, 996, 1119
BtsCI GGATG 3 cut(s) 385, 649, 949
BtsIMutI CAGTG 4 cut(s) 127, 175, 252, 560
Cac8I GCNNGC 3 cut(s) 124, 371, 1055
CfoI GCGC 4 cut(s) 69, 845, 1109, 1157
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 690, 729
CviAII CATG 5 cut(s) 80, 451, 497, 1120, 1191
CviQI GTAC 2 cut(s) 690, 729
DdeI CTNAG 8 cut(s) 230, 238, 360, 375, 429, 437, 967, 1110
DpnI GATC 3 cut(s) 198, 700, 746
DpnII GATC 3 cut(s) 196, 698, 744
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 43, 1119
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 679
Eco81I CCTNAGG 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 805
EcoRII CCWGG 2 cut(s) 925, 1101
EcoT14I CCWWGG 2 cut(s) 43, 1119
ErhI CCWWGG 2 cut(s) 43, 1119
FaeI CATG 5 cut(s) 83, 454, 500, 1123, 1194
FalI AAGNNNNNCTT 2 cut(s) 1012, 1044
FaqI GGGAC 1 cut(s) 491
FatI CATG 5 cut(s) 79, 450, 496, 1119, 1190
FauNDI CATATG 1 cut(s) 815
Fnu4HI GCNGC 5 cut(s) 227, 367, 841, 891, 1058
FokI GGATG 3 cut(s) 392, 636, 956
Fsp4HI GCNGC 5 cut(s) 227, 367, 841, 891, 1058
FspBI CTAG 4 cut(s) 573, 726, 807, 1098
GlaI GCGC 4 cut(s) 68, 844, 1108, 1156
GluI GCNGC 5 cut(s) 227, 367, 841, 891, 1058
GsuI CTGGAG 1 cut(s) 151
HaeII RGCGCY 2 cut(s) 1110, 1158
HaeIII GGCC 5 cut(s) 36, 300, 800, 1046, 1053
HapII CCGG 2 cut(s) 21, 179
HhaI GCGC 4 cut(s) 69, 845, 1109, 1157
Hin1II CATG 5 cut(s) 83, 454, 500, 1123, 1194
Hin6I GCGC 4 cut(s) 67, 843, 1107, 1155
HinP1I GCGC 4 cut(s) 67, 843, 1107, 1155
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HinfI GANTC 4 cut(s) 17, 191, 490, 711
HpaII CCGG 2 cut(s) 21, 179
HphI GGTGA 2 cut(s) 16, 749
Hpy166II GTNNAC 2 cut(s) 567, 647
Hpy188III TCNNGA 4 cut(s) 216, 428, 748, 1020
Hpy8I GTNNAC 2 cut(s) 567, 647
Hpy99I CGWCG 2 cut(s) 55, 104
HpyAV CCTTC 3 cut(s) 703, 1002, 1038
HpyCH4III ACNGT 2 cut(s) 131, 564
HpyCH4IV ACGT 2 cut(s) 905, 977
HpyCH4V TGCA 5 cut(s) 257, 369, 831, 987, 1190
HpyF10VI GCNNNNNNNGC 8 cut(s) 170, 493, 797, 819, 828, 837, 896, 1115
HpyF3I CTNAG 8 cut(s) 230, 238, 360, 375, 429, 437, 967, 1110
HpySE526I ACGT 2 cut(s) 905, 977
Hsp92II CATG 5 cut(s) 83, 454, 500, 1123, 1194
HspAI GCGC 4 cut(s) 67, 843, 1107, 1155
Kzo9I GATC 3 cut(s) 196, 698, 744
LguI GCTCTTC 1 cut(s) 116
LmnI GCTCC 1 cut(s) 819
Lsp1109I GCAGC 5 cut(s) 238, 353, 852, 877, 1044
LweI GCATC 3 cut(s) 244, 474, 757
MaeI CTAG 4 cut(s) 573, 726, 807, 1098
MaeII ACGT 2 cut(s) 905, 977
MaeIII GTNAC 5 cut(s) 247, 403, 504, 901, 1091
MalI GATC 3 cut(s) 198, 700, 746
MboI GATC 3 cut(s) 196, 698, 744
MboII GAAGA 4 cut(s) 103, 304, 682, 776
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 1 cut(s) 111
MlsI TGGCCA 1 cut(s) 1046
MluCI AATT 8 cut(s) 8, 59, 418, 794, 909, 1013, 1037, 1179
MluNI TGGCCA 1 cut(s) 1046
MmeI TCCRAC 2 cut(s) 33, 521
Mox20I TGGCCA 1 cut(s) 1046
MroXI GAANNNNTTC 2 cut(s) 75, 1027
MscI TGGCCA 1 cut(s) 1046
MseI TTAA 5 cut(s) 776, 849, 908, 912, 1133
Msp20I TGGCCA 1 cut(s) 1046
MspI CCGG 2 cut(s) 21, 179
MspR9I CCNGG 2 cut(s) 927, 1103
MvaI CCWGG 2 cut(s) 927, 1103
MvnI CGCG 2 cut(s) 14, 69
MwoI GCNNNNNNNGC 8 cut(s) 170, 493, 797, 819, 828, 837, 896, 1115
NcoI CCATGG 1 cut(s) 1119
NdeI CATATG 1 cut(s) 815
NdeII GATC 3 cut(s) 196, 698, 744
NlaIII CATG 5 cut(s) 83, 454, 500, 1123, 1194
NlaIV GGNNCC 2 cut(s) 660, 921
NmuCI GTSAC 4 cut(s) 247, 403, 901, 1091
PacI TTAATTAA 1 cut(s) 912
PciSI GCTCTTC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdmI GAANNNNTTC 2 cut(s) 75, 1027
PfeI GAWTC 4 cut(s) 17, 191, 490, 711
PkrI GCNGC 5 cut(s) 228, 368, 842, 892, 1059
Psp6I CCWGG 2 cut(s) 925, 1101
PspGI CCWGG 2 cut(s) 925, 1101
PspN4I GGNNCC 2 cut(s) 660, 921
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 698
RsaI GTAC 2 cut(s) 691, 730
RsaNI GTAC 2 cut(s) 690, 729
SapI GCTCTTC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 776, 849, 908, 912, 1133
SatI GCNGC 5 cut(s) 227, 367, 841, 891, 1058
Sau3AI GATC 3 cut(s) 196, 698, 744
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 730
ScrFI CCNGG 2 cut(s) 927, 1103
SduI GDGCHC 1 cut(s) 111
SfaNI GCATC 3 cut(s) 244, 474, 757
SinI GGWCC 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 397, 957
SmoI CTYRAG 2 cut(s) 397, 957
Sse9I AATT 8 cut(s) 8, 59, 418, 794, 909, 1013, 1037, 1179
SsiI CCGC 3 cut(s) 14, 84, 224
SspMI CTAG 4 cut(s) 573, 726, 807, 1098
StyD4I CCNGG 2 cut(s) 925, 1101
StyI CCWWGG 2 cut(s) 43, 1119
TaaI ACNGT 2 cut(s) 131, 564
TaiI ACGT 2 cut(s) 908, 980
TaqI TCGA 2 cut(s) 217, 860
TasI AATT 8 cut(s) 8, 59, 418, 794, 909, 1013, 1037, 1179
TatI WGTACW 1 cut(s) 728
TfiI GAWTC 4 cut(s) 17, 191, 490, 711
Tru1I TTAA 5 cut(s) 776, 849, 908, 912, 1133
Tru9I TTAA 5 cut(s) 776, 849, 908, 912, 1133
TscAI CASTG 4 cut(s) 134, 175, 252, 567
TseFI GTSAC 4 cut(s) 247, 403, 901, 1091
TseI GCWGC 5 cut(s) 226, 366, 840, 890, 1057
Tsp45I GTSAC 4 cut(s) 247, 403, 901, 1091
TspDTI ATGAA 3 cut(s) 68, 960, 1149
TspGWI ACGGA 1 cut(s) 956
TspRI CASTG 4 cut(s) 134, 175, 252, 567
VpaK11BI GGWCC 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 805
XapI RAATTY 3 cut(s) 8, 59, 1179
XcmI CCANNNNNNNNNTGG 1 cut(s) 767
XmnI GAANNNNTTC 2 cut(s) 75, 1027
XspI CTAG 4 cut(s) 573, 726, 807, 1098
ZrmI AGTACT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.