Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0002322

Physical Location & Seq

Genomic Coordinates Reverse (-)

32467403 .. 32478436

Gene Structure 11034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0283300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGGAGGAGGAAATGGACGAAACCGAAGCTCTACCTCAGGTGTACATGGCATGCATTCGACAAGGTCATCGAGTGGGAGTTTCTTATTATGATTCCAGTATTCGCCAACTTCACGTGATGGAAGTTTGGGAAGATGGCAGCATGGACTTCCCCCTAATTGATATGGTGAAATATCAAGCTAAGCCACTGATCATCTACACAAGCACAAAAAGCGAGGAAGCTTTCTTGTCTGCTTTACAACGAAGTGATGGAACAACAGAGGCTTTTACTGTGAAGCTTGTGAAAAGTTCAGTATTTAGCTATGAGCAAGCATGGCACAGATTGATATACCTTCGAGTAACAGGAATGGATGATGGGTTAGACATCAAGGAGAGAATTTGTTATTTGAGTTCTATGATGGACATGGGAAGTGATGTTCAAGTTCGTACCAGTGGAGGCCTTCTTGCCATATTAGAGAATGAAAGGATTGTAGACACACTTGAACAAATGGAATGCGGCAATGCATCTATTGCAATTGATTCTGTTATAGAAGTTTCATTAAACAATTTTCTAAAACTTGATGCTGCAGCTCATGAGGCGTTGCAGATATTCCAAACAGATAAGCATCCTAGCCATATGGGCATTGGTAGAGCAAAAGAAGGGTTTTCTGTATTTGGCATGTTGAATAAGTGTGTAACTCCTATTGGGAGGCGCCTCTTGAGGAACTGGTTCCTAAGGCCAATAATTGACCTTGAAATTTTGAACAGTCGTCTTGATACCATATCATTCTTTATATCTTCAGAAGAACTAATTATGTCTTTACGGTCAACTCTGAAATCTGTTAAGGATATTCCTCACATACTCAAGAAATTCAACTCCCCAAGCTCCGTATGTACCAGTACCGATTGGACAGCTTTTTTAAAGAGTGTTTGCTCACTCTTGCACGTGAACAAGATATTTGAAGTGGGTGTTTCTGAGAGTCTTCAAGAGCATGCCAAGTACCTGAATTTGGACATTTTTGAGAAGGCTGCTTCATGCATTACAACTGAGCTTGCTTATGTATATGAATTGGTAATTGGTGTTCTTGACGTAAACAGAAGTAAAGAGAAGGGATATGAGACAATTGTAAAAGATGACTTCTGTACTGAGTTAGATGAGCTGAGGCAAATATATGAGGAATTGCCCGAATTTCTGGAAGAGGTTTCAAAGATGGAACTTGGAAGACTTCCTCAGCTGTGCAAAGACAAGCTTGTTCCTTGTATCGTGTATATACACCAGATAGGATACTTGATGTGCATTTTTGAAGAAAAACTTGATGAAGCTACCCTGGAGACACTTCAAGATTTTGAGTTTGCTTTCTCTGATGCAGAAGGAGAAACAAAAAGATTCTTTTATCGTACTCCAAAGACACAAGAGTTGGATAATCTTCTGGGAGATATCTATCATAAAATACTAGATATGGAGAGGGCGATCACTCGAGATCTGGTGTCACATATACTTCTATTTTCAACGCATCTGCTTAAAGCTGTAAATTTTGCAGCTGAACTTGATTGCTTTTTGTCATTGGCGCTGGTTGCCCGTCAAAACAATTATGTAAGGCCTACTTTGACCACGGACTGCTTGCTTGATATACAAAATGGAAGACATGTTTTGCAGGAAATGACGGTAGACACTTTTGTTCCCAATGATACAAAGATTCAAAATGATGGGAGAATTAATATCATCACTGGTCCCAACTATTCAGGGAAAAGCATCTACATAAAGCAGGTAGCTTTGATTGTTTTCCTAGCCCATATAGGAAGTTTTGTTCCGGCAGATGCTGCAACTGTGGGTTTGACTGATAGAATATTCTGCGCAATGGGAAGCAAGCTCATGGCTGCAGAACAATCAACGTTCATGATTGATCTGCATCAAGTAGGGATGATGCTAAGGCAGGCAACTTCACGGTCTTTGTGTCTCTTGGATGAATTTGGTAAAGGTACTCTTACAGAAGATGGCGTTGGTCTGCTTGGTGGAACCATAAACTACTTGGTGTCCTATGATGACCCCCCAAAGGTTCTAGTGTGCACACATTTGACTGAGTTATTCAGCGAGAGTTGCTTACCTAAATCCAAGAAGATCACATTTTACACTATGAGTGTGCTGAGGCCTGATAACTCAACAGATATTGAGGATATTGTTTTCCTATATAGGTTAGTTCCTGGGCATGCACTACGTAGTTATGGTCTTCATTGTGCTCTACTTGCTGGTGTCCCAGAGGAAGTTATCAAGAGAGCAGCATTTGTATTGGAAGCGATTGGAAATAATCAGCATGTCGAGCGATTGTGCAATGAGAATTTATCAGTTCAGGATCAGCAATACCAGACAGTAGTGGACAAAATGTTGGCATTTGATGTTCACAATGGCGATTTAGGCCTGTTCTTTCAGGACTTATTTTCTCCTGCGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

YTH

Protein Analysis

809

Amino Acids

91.34

Weight (kDa)

4.99

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_III	PF05192	190 - 516	3.3e-31	MutS domain III
MutS_V	PF00488	567 - 758	1.5e-48	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002322)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55960 AT1G55960 AT3G13062 AT3G13062 AT3G13062
fragaria_vesca	FvH4_6g36480 FvH4_6g36480
malus_domestica	MD09G1168600.v1.1 MD10G1168000.v1.1 MD17G1158100.v1.1
prunus_persica	Prupe.3G027000_v2.0.a1
pyrus_communis	pycom09g08630 pycom17g15160
rosa_chinensis	RchiOBHm_Chr1g0356291 RchiOBHm_Chr1g0356301 RchiOBHm_Chr2g0148611
rosa_laevigata	RLG00000020355
rosa_multiflora	Rmu_sc0000048.1_g000024 Rmu_sc0007842.1_g000001 Rmu_ssc0000368.1_g000067
rosa_roxburghii	Rroxscaffold_2G00098580 Rroxscaffold_5G00343750
rosa_rugosa	Rorug02G0303100 Rorug02G0303200 Rorug02G0408100 Rorug05G0283200 Rorug05G0283300
rosa_samantha	Rh2AG466500 Rh2BG479600 Rh2CG453000 Rh2DG488400 Rh3DG124100 Rh4AG089800 Rh4AG118200 Rh4AG189300 Rh4CG097800 Rh5DG503900 Rh6CG162100 Rh6DG153700 Rh7BG309800 Rh7DG365700
rosa_wichuraiana	Rw2G038040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1834
Acc36I	ACCTGC	1 cut(s)	1735
AccB1I	GGYRCC	1 cut(s)	690
AccI	GTMKAC	2 cut(s)	471, 1647
AciI	CCGC	1 cut(s)	495
AclI	AACGTT	1 cut(s)	1871
AclWI	GGATC	1 cut(s)	2337
AcsI	RAATTY	8 cut(s)	375, 735, 848, 985, 1166, 1510, 1946, 2314
AcuI	CTGAAG	1 cut(s)	762
AcvI	CACGTG	2 cut(s)	115, 925
AcyI	GRCGYC	1 cut(s)	691
AfaI	GTAC	8 cut(s)	44, 427, 874, 880, 980, 1123, 1378, 1960
AfiI	CCNNNNNNNGG	2 cut(s)	988, 2032
AflIII	ACRYGT	1 cut(s)	1624
AjnI	CCWGG	2 cut(s)	1305, 2179
AjuI	GAANNNNNNNTTGG	4 cut(s)	1038, 1070, 1962, 1994
AloI	GAACNNNNNNTCC	4 cut(s)	399, 431, 1770, 1802
Alw21I	GWGCWC	2 cut(s)	2048, 2218
Alw26I	GTCTC	3 cut(s)	1091, 1304, 1940
Alw44I	GTGCAC	1 cut(s)	2044
AlwI	GGATC	1 cut(s)	2337
AlwNI	CAGNNNCTG	1 cut(s)	1799
Ama87I	CYCGRG	1 cut(s)	1455
AoxI	GGCC	5 cut(s)	436, 716, 1577, 2126, 2392
ApaLI	GTGCAC	1 cut(s)	2044
ApeKI	GCWGC	8 cut(s)	138, 563, 566, 1007, 1517, 1799, 1856, 2255
ApoI	RAATTY	8 cut(s)	375, 735, 848, 985, 1166, 1510, 1946, 2314
AseI	ATTAAT	1 cut(s)	1695
Asp700I	GAANNNNTTC	1 cut(s)	707
AspLEI	GCGC	3 cut(s)	693, 1549, 1835
AspS9I	GGNCC	1 cut(s)	1709
AsuHPI	GGTGA	1 cut(s)	178
AvaI	CYCGRG	1 cut(s)	1455
AvaII	GGWCC	1 cut(s)	1709
AxyI	CCTNAGG	2 cut(s)	36, 713
BaeGI	GKGCMC	1 cut(s)	2048
BanI	GGYRCC	1 cut(s)	690
BbrPI	CACGTG	2 cut(s)	115, 925
BbsI	GAAGAC	4 cut(s)	953, 1207, 1627, 2198
Bbv12I	GWGCWC	2 cut(s)	2048, 2218
BbvCI	CCTCAGC	3 cut(s)	1139, 1209, 2123
BbvI	GCAGC	8 cut(s)	150, 550, 578, 994, 1529, 1786, 1843, 2267
BccI	CCATC	8 cut(s)	112, 128, 242, 347, 391, 1183, 1679, 1967
BciT130I	CCWGG	2 cut(s)	1307, 2181
BciVI	GTATCC	1 cut(s)	1256
BclI	TGATCA	1 cut(s)	189
BcoDI	GTCTC	3 cut(s)	1091, 1304, 1940
BfaI	CTAG	5 cut(s)	609, 1433, 1766, 2039, 2428
BfmI	CTRYAG	2 cut(s)	564, 1857
BfoI	RGCGCY	2 cut(s)	694, 1550
BfuAI	ACCTGC	1 cut(s)	1735
BfuI	GTATCC	1 cut(s)	1256
BglI	GCCNNNNNGGC	1 cut(s)	618
BglII	AGATCT	1 cut(s)	1459
BisI	GCNGC	9 cut(s)	139, 496, 564, 567, 1008, 1518, 1800, 1857, 2256
BlpI	GCTNAGC	1 cut(s)	180
BlsI	GCNGC	9 cut(s)	140, 497, 565, 568, 1009, 1519, 1801, 1858, 2257
Bme1390I	CCNGG	2 cut(s)	1307, 2181
Bme18I	GGWCC	1 cut(s)	1709
BmeT110I	CYCGRG	1 cut(s)	1455
BmgT120I	GGNCC	1 cut(s)	1709
BmiI	GGNNCC	4 cut(s)	692, 710, 1711, 1996
BmrFI	CCNGG	2 cut(s)	1307, 2181
BmsI	GCATC	9 cut(s)	512, 550, 613, 1333, 1501, 1740, 1786, 1893, 1897
BpiI	GAAGAC	4 cut(s)	953, 1207, 1627, 2198
BpmI	CTGGAG	1 cut(s)	1328
Bpu10I	CCTNAGC	4 cut(s)	1139, 1209, 1907, 2123
Bpu1102I	GCTNAGC	1 cut(s)	180
BpuEI	CTTGAG	2 cut(s)	718, 827
BsaAI	YACGTR	3 cut(s)	115, 925, 2195
BsaBI	GATNNNNATC	3 cut(s)	603, 1419, 1887
BsaHI	GRCGYC	1 cut(s)	691
BsaJI	CCNNGG	3 cut(s)	1305, 1590, 2180
Bsc4I	CCNNNNNNNGG	2 cut(s)	988, 2032
Bse1I	ACTGG	5 cut(s)	96, 429, 710, 876, 1711
Bse21I	CCTNAGG	2 cut(s)	36, 713
Bse3DI	GCAATG	3 cut(s)	505, 1842, 2314
Bse8I	GATNNNNATC	3 cut(s)	603, 1419, 1887
BseBI	CCWGG	2 cut(s)	1307, 2181
BseDI	CCNNGG	3 cut(s)	1305, 1590, 2180
BseGI	GGATG	4 cut(s)	355, 604, 1905, 1948
BseJI	GATNNNNATC	3 cut(s)	603, 1419, 1887
BseLI	CCNNNNNNNGG	2 cut(s)	988, 2032
BseMI	GCAATG	3 cut(s)	505, 1842, 2314
BseMII	CTCAG	8 cut(s)	50, 945, 1017, 1116, 1130, 1223, 2049, 2114
BseNI	ACTGG	5 cut(s)	96, 429, 710, 876, 1711
BseRI	GAGGAG	1 cut(s)	20
BseSI	GKGCMC	1 cut(s)	2048
BseXI	GCAGC	8 cut(s)	150, 550, 578, 994, 1529, 1786, 1843, 2267
BshFI	GGCC	5 cut(s)	438, 718, 1579, 2128, 2394
BshNI	GGYRCC	1 cut(s)	690
BsiHKAI	GWGCWC	2 cut(s)	2048, 2218
BsiHKCI	CYCGRG	1 cut(s)	1455
BsiSI	CCGG	1 cut(s)	1790
BslFI	GGGAC	2 cut(s)	1695, 2216
BslI	CCNNNNNNNGG	2 cut(s)	988, 2032
BsmAI	GTCTC	3 cut(s)	1091, 1304, 1940
BsmFI	GGGAC	2 cut(s)	1695, 2216
BsmI	GAATGC	2 cut(s)	54, 497
BsnI	GGCC	5 cut(s)	438, 718, 1579, 2128, 2394
BsoBI	CYCGRG	1 cut(s)	1455
Bsp1286I	GDGCHC	2 cut(s)	2048, 2218
Bsp1407I	TGTACA	1 cut(s)	42
Bsp143I	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
Bsp1720I	GCTNAGC	1 cut(s)	180
BspACI	CCGC	1 cut(s)	495
BspANI	GGCC	5 cut(s)	438, 718, 1579, 2128, 2394
BspCNI	CTCAG	8 cut(s)	49, 946, 1018, 1117, 1131, 1222, 2050, 2115
BspHI	TCATGA	2 cut(s)	571, 1875
BspLI	GGNNCC	4 cut(s)	692, 710, 1711, 1996
BspMAI	CTGCAG	2 cut(s)	568, 1861
BspMI	ACCTGC	1 cut(s)	1735
BspPI	GGATC	1 cut(s)	2337
BspT107I	GGYRCC	1 cut(s)	690
BsrDI	GCAATG	3 cut(s)	505, 1842, 2314
BsrGI	TGTACA	1 cut(s)	42
BsrI	ACTGG	5 cut(s)	96, 429, 710, 876, 1711
BssECI	CCNNGG	3 cut(s)	1305, 1590, 2180
BssMI	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
BssNI	GRCGYC	1 cut(s)	691
Bst2UI	CCWGG	2 cut(s)	1307, 2181
Bst4CI	ACNGT	7 cut(s)	271, 746, 804, 1645, 1807, 1926, 2347
Bst6I	CTCTTC	1 cut(s)	1170
BstACI	GRCGYC	1 cut(s)	691
BstAPI	GCANNNNNTGC	2 cut(s)	509, 1799
BstAUI	TGTACA	1 cut(s)	42
BstBAI	YACGTR	3 cut(s)	115, 925, 2195
BstC8I	GCNNGC	8 cut(s)	52, 309, 972, 1032, 1601, 1847, 1914, 2187
BstDSI	CCRYGG	1 cut(s)	1590
BstF5I	GGATG	4 cut(s)	355, 604, 1905, 1948
BstH2I	RGCGCY	2 cut(s)	694, 1550
BstHHI	GCGC	3 cut(s)	693, 1549, 1835
BstKTI	GATC	6 cut(s)	192, 1452, 1462, 1885, 2100, 2332
BstMAI	GTCTC	3 cut(s)	1091, 1304, 1940
BstMBI	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
BstNI	CCWGG	2 cut(s)	1307, 2181
BstNSI	RCATGY	6 cut(s)	54, 661, 974, 1628, 2189, 2294
BstSCI	CCNGG	2 cut(s)	1305, 2179
BstSFI	CTRYAG	2 cut(s)	564, 1857
BstSLI	GKGCMC	1 cut(s)	2048
BstSNI	TACGTA	1 cut(s)	2195
BstV1I	GCAGC	8 cut(s)	150, 550, 578, 994, 1529, 1786, 1843, 2267
BstV2I	GAAGAC	4 cut(s)	953, 1207, 1627, 2198
BstX2I	RGATCY	1 cut(s)	1459
BstYI	RGATCY	1 cut(s)	1459
Bsu36I	CCTNAGG	2 cut(s)	36, 713
BsuI	GTATCC	1 cut(s)	1256
BsuRI	GGCC	5 cut(s)	438, 718, 1579, 2128, 2394
BtgI	CCRYGG	1 cut(s)	1590
BtsCI	GGATG	4 cut(s)	355, 604, 1905, 1948
BtsIMutI	CAGTG	3 cut(s)	185, 436, 1704
BveI	ACCTGC	1 cut(s)	1735
Cac8I	GCNNGC	8 cut(s)	52, 309, 972, 1032, 1601, 1847, 1914, 2187
CaiI	CAGNNNCTG	1 cut(s)	1799
CciI	TCATGA	2 cut(s)	571, 1875
CfoI	GCGC	3 cut(s)	693, 1549, 1835
Cfr13I	GGNCC	1 cut(s)	1709
CseI	GACGC	1 cut(s)	2412
Csp6I	GTAC	8 cut(s)	43, 426, 873, 879, 979, 1122, 1377, 1959
CviQI	GTAC	8 cut(s)	43, 426, 873, 879, 979, 1122, 1377, 1959
DinI	GGCGCC	1 cut(s)	692
DpnI	GATC	6 cut(s)	191, 1451, 1461, 1884, 2099, 2331
DpnII	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
DraI	TTTAAA	1 cut(s)	900
Eam1104I	CTCTTC	1 cut(s)	1170
EarI	CTCTTC	1 cut(s)	1170
Eco105I	TACGTA	1 cut(s)	2195
Eco147I	AGGCCT	4 cut(s)	438, 1579, 2128, 2394
Eco32I	GATATC	1 cut(s)	1417
Eco47I	GGWCC	1 cut(s)	1709
Eco57I	CTGAAG	1 cut(s)	762
Eco72I	CACGTG	2 cut(s)	115, 925
Eco81I	CCTNAGG	2 cut(s)	36, 713
Eco88I	CYCGRG	1 cut(s)	1455
EcoRII	CCWGG	2 cut(s)	1305, 2179
EcoRV	GATATC	1 cut(s)	1417
EcoT22I	ATGCAT	3 cut(s)	56, 505, 1019
EgeI	GGCGCC	1 cut(s)	692
EheI	GGCGCC	1 cut(s)	692
FalI	AAGNNNNNCTT	8 cut(s)	1212, 1244, 1275, 1307, 1567, 1599, 1947, 1979
FaqI	GGGAC	2 cut(s)	1695, 2216
FauNDI	CATATG	1 cut(s)	615
FbaI	TGATCA	1 cut(s)	189
FblI	GTMKAC	2 cut(s)	471, 1647
Fnu4HI	GCNGC	9 cut(s)	139, 496, 564, 567, 1008, 1518, 1800, 1857, 2256
FokI	GGATG	4 cut(s)	362, 591, 1912, 1955
Fsp4HI	GCNGC	9 cut(s)	139, 496, 564, 567, 1008, 1518, 1800, 1857, 2256
FspBI	CTAG	5 cut(s)	609, 1433, 1766, 2039, 2428
FspI	TGCGCA	1 cut(s)	1834
GlaI	GCGC	3 cut(s)	692, 1548, 1834
GluI	GCNGC	9 cut(s)	139, 496, 564, 567, 1008, 1518, 1800, 1857, 2256
GsuI	CTGGAG	1 cut(s)	1328
HaeII	RGCGCY	2 cut(s)	694, 1550
HaeIII	GGCC	5 cut(s)	438, 718, 1579, 2128, 2394
HapII	CCGG	1 cut(s)	1790
HgaI	GACGC	1 cut(s)	2412
HhaI	GCGC	3 cut(s)	693, 1549, 1835
Hin1I	GRCGYC	1 cut(s)	691
Hin6I	GCGC	3 cut(s)	691, 1547, 1833
HinP1I	GCGC	3 cut(s)	691, 1547, 1833
HincII	GTYRAC	1 cut(s)	807
HindII	GTYRAC	1 cut(s)	807
HindIII	AAGCTT	3 cut(s)	219, 275, 1226
HinfI	GANTC	5 cut(s)	92, 518, 958, 1365, 1675
HpaII	CCGG	1 cut(s)	1790
HphI	GGTGA	1 cut(s)	178
Hpy166II	GTNNAC	9 cut(s)	43, 472, 807, 928, 1072, 1648, 2046, 2353, 2377
Hpy188I	TCNGA	4 cut(s)	781, 813, 955, 1342
Hpy8I	GTNNAC	9 cut(s)	43, 472, 807, 928, 1072, 1648, 2046, 2353, 2377
HpyAV	CCTTC	6 cut(s)	341, 449, 632, 997, 1081, 1343
HpyCH4III	ACNGT	7 cut(s)	271, 746, 804, 1645, 1807, 1926, 2347
HpyCH4IV	ACGT	5 cut(s)	114, 924, 1068, 1871, 2194
HpySE526I	ACGT	5 cut(s)	114, 924, 1068, 1871, 2194
Hsp92I	GRCGYC	1 cut(s)	691
HspAI	GCGC	3 cut(s)	691, 1547, 1833
KasI	GGCGCC	1 cut(s)	690
Ksp22I	TGATCA	1 cut(s)	189
Kzo9I	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
LmnI	GCTCC	1 cut(s)	869
Lsp1109I	GCAGC	8 cut(s)	150, 550, 578, 994, 1529, 1786, 1843, 2267
LweI	GCATC	9 cut(s)	512, 550, 613, 1333, 1501, 1740, 1786, 1893, 1897
MaeI	CTAG	5 cut(s)	609, 1433, 1766, 2039, 2428
MaeII	ACGT	5 cut(s)	114, 924, 1068, 1871, 2194
MaeIII	GTNAC	3 cut(s)	337, 673, 1467
MalI	GATC	6 cut(s)	191, 1451, 1461, 1884, 2099, 2331
MboI	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
MfeI	CAATTG	2 cut(s)	513, 1101
MflI	RGATCY	1 cut(s)	1459
MhlI	GDGCHC	2 cut(s)	2048, 2218
Mly113I	GGCGCC	1 cut(s)	691
MlyI	GAGTC	1 cut(s)	967
MmeI	TCCRAC	1 cut(s)	1377
Mph1103I	ATGCAT	3 cut(s)	56, 505, 1019
MroXI	GAANNNNTTC	1 cut(s)	707
MseI	TTAA	5 cut(s)	539, 822, 899, 1500, 1695
MspA1I	CMGCKG	2 cut(s)	1213, 1520
MspI	CCGG	1 cut(s)	1790
MspR9I	CCNGG	2 cut(s)	1307, 2181
MunI	CAATTG	2 cut(s)	513, 1101
Mva1269I	GAATGC	2 cut(s)	54, 497
MvaI	CCWGG	2 cut(s)	1307, 2181
NarI	GGCGCC	1 cut(s)	691
NdeI	CATATG	1 cut(s)	615
NdeII	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
NlaIV	GGNNCC	4 cut(s)	692, 710, 1711, 1996
NmuCI	GTSAC	1 cut(s)	1467
NsbI	TGCGCA	1 cut(s)	1834
NsiI	ATGCAT	3 cut(s)	56, 505, 1019
NspI	RCATGY	6 cut(s)	54, 661, 974, 1628, 2189, 2294
PaeI	GCATGC	3 cut(s)	54, 974, 2189
PaeR7I	CTCGAG	1 cut(s)	1455
PagI	TCATGA	2 cut(s)	571, 1875
PceI	AGGCCT	4 cut(s)	438, 1579, 2128, 2394
PciI	ACATGT	1 cut(s)	1624
PctI	GAATGC	2 cut(s)	54, 497
PdmI	GAANNNNTTC	1 cut(s)	707
PfeI	GAWTC	4 cut(s)	92, 518, 1365, 1675
PflFI	GACNNNGTC	1 cut(s)	63
PkrI	GCNGC	9 cut(s)	140, 497, 565, 568, 1009, 1519, 1801, 1858, 2257
PleI	GAGTC	1 cut(s)	966
PluTI	GGCGCC	1 cut(s)	694
PmaCI	CACGTG	2 cut(s)	115, 925
PmlI	CACGTG	2 cut(s)	115, 925
PpsI	GAGTC	1 cut(s)	966
Ppu21I	YACGTR	3 cut(s)	115, 925, 2195
PscI	ACATGT	1 cut(s)	1624
PshBI	ATTAAT	1 cut(s)	1695
Psp1406I	AACGTT	1 cut(s)	1871
Psp6I	CCWGG	2 cut(s)	1305, 2179
PspCI	CACGTG	2 cut(s)	115, 925
PspGI	CCWGG	2 cut(s)	1305, 2179
PspN4I	GGNNCC	4 cut(s)	692, 710, 1711, 1996
PspPI	GGNCC	1 cut(s)	1709
PsrI	GAACNNNNNNTAC	2 cut(s)	1044, 1076
PstI	CTGCAG	2 cut(s)	568, 1861
PstNI	CAGNNNCTG	1 cut(s)	1799
PsuI	RGATCY	1 cut(s)	1459
PsyI	GACNNNGTC	1 cut(s)	63
PvuII	CAGCTG	2 cut(s)	1213, 1520
RsaI	GTAC	8 cut(s)	44, 427, 874, 880, 980, 1123, 1378, 1960
RsaNI	GTAC	8 cut(s)	43, 426, 873, 879, 979, 1122, 1377, 1959
SaqAI	TTAA	5 cut(s)	539, 822, 899, 1500, 1695
SatI	GCNGC	9 cut(s)	139, 496, 564, 567, 1008, 1518, 1800, 1857, 2256
Sau3AI	GATC	6 cut(s)	189, 1449, 1459, 1882, 2097, 2329
Sau96I	GGNCC	1 cut(s)	1709
SchI	GAGTC	1 cut(s)	967
ScrFI	CCNGG	2 cut(s)	1307, 2181
SduI	GDGCHC	2 cut(s)	2048, 2218
SfaNI	GCATC	9 cut(s)	512, 550, 613, 1333, 1501, 1740, 1786, 1893, 1897
SfcI	CTRYAG	2 cut(s)	564, 1857
SfoI	GGCGCC	1 cut(s)	692
Sfr274I	CTCGAG	1 cut(s)	1455
SinI	GGWCC	1 cut(s)	1709
SlaI	CTCGAG	1 cut(s)	1455
SmlI	CTYRAG	3 cut(s)	697, 842, 1455
SmoI	CTYRAG	3 cut(s)	697, 842, 1455
SnaBI	TACGTA	1 cut(s)	2195
SphI	GCATGC	3 cut(s)	54, 974, 2189
SseBI	AGGCCT	4 cut(s)	438, 1579, 2128, 2394
SsiI	CCGC	1 cut(s)	495
SspDI	GGCGCC	1 cut(s)	690
SspI	AATATT	1 cut(s)	1827
SspMI	CTAG	5 cut(s)	609, 1433, 1766, 2039, 2428
StuI	AGGCCT	4 cut(s)	438, 1579, 2128, 2394
StyD4I	CCNGG	2 cut(s)	1305, 2179
TaaI	ACNGT	7 cut(s)	271, 746, 804, 1645, 1807, 1926, 2347
TaiI	ACGT	5 cut(s)	117, 927, 1071, 1874, 2197
TaqI	TCGA	5 cut(s)	58, 70, 334, 1456, 2295
TatI	WGTACW	2 cut(s)	42, 1121
TauI	GCSGC	1 cut(s)	498
TfiI	GAWTC	4 cut(s)	92, 518, 1365, 1675
Tru1I	TTAA	5 cut(s)	539, 822, 899, 1500, 1695
Tru9I	TTAA	5 cut(s)	539, 822, 899, 1500, 1695
TscAI	CASTG	3 cut(s)	192, 436, 1711
TseFI	GTSAC	1 cut(s)	1467
TseI	GCWGC	8 cut(s)	138, 563, 566, 1007, 1517, 1799, 1856, 2255
Tsp45I	GTSAC	1 cut(s)	1467
TspDTI	ATGAA	8 cut(s)	474, 525, 1002, 1059, 1311, 1864, 1959, 2198
TspGWI	ACGGA	2 cut(s)	856, 1607
TspRI	CASTG	3 cut(s)	192, 436, 1711
Tth111I	GACNNNGTC	1 cut(s)	63
VneI	GTGCAC	1 cut(s)	2044
VpaK11BI	GGWCC	1 cut(s)	1709
VspI	ATTAAT	1 cut(s)	1695
XapI	RAATTY	8 cut(s)	375, 735, 848, 985, 1166, 1510, 1946, 2314
XceI	RCATGY	6 cut(s)	54, 661, 974, 1628, 2189, 2294
XcmI	CCANNNNNNNNNTGG	2 cut(s)	620, 2005
XhoI	CTCGAG	1 cut(s)	1455
XmiI	GTMKAC	2 cut(s)	471, 1647
XmnI	GAANNNNTTC	1 cut(s)	707
XspI	CTAG	5 cut(s)	609, 1433, 1766, 2039, 2428
Zsp2I	ATGCAT	3 cut(s)	56, 505, 1019

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.