AT4G28070

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001671
Physical Location & Seq
Forward (+)
13945892 .. 13948627
2736 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28070.2

Sequence Viewer

Length: 1395 bp
ATGCGATTGCTTCTTCTCTCCCTTCGCCGTGTAACTCTATTCTCGAGGCATCAACGATACGCTTTCGCTCCCATTCATAATTGTTTGATCTCTATCAATCCCAGCACATCTTCTTCATCTCTATTCTCGGCTTCTTCCTTCTGTTCGTCTCCACATTCCAATGGAGATGGAAAGATTGCAGGGCCGTTAGTGGAGTATGAACGCAGAATCGTAGCTGGAGAGTTATTGGATGGAGATTTATGTCAGCTAGGTACACTAAGAGAACTTCAGAGACTCTACGACGAGCTTGTTCAATCTGCTGATGCTTGTCGATTGGATCGTTATTCTGCATCTGCAAAGTCCACTAGGAGTAATTGGTTCTGGAACAAATTTGTGTCTCACTCTTCTGTCTCTCCTGTCAAAGGCTTATACTTATATGGAGGAGTAGGAACTGGAAAGACAATGCTCATGGACTTGTTTTTTCATCAATTGCCAGCTAGCTGGAGAACACAAAGGATTCACTTTCACAATTTCATGTTGAGTGTGCATAGCCGCCTGCAAAAGCACAAGGGTCTTGAAGATCCACTTGAAGTCGTTGGCCTGGAAATAGCTGACGAGGCTATATTACTATGCTTAGATGAGTTCATGGTCAATGATGTAGCTGATGCACTGATACTAAACCGTCTCTTTAGACACTTGTTTAACAATGGCATTATTCTTGTTGCTACATCAAACCGTGCTCCAGATAATCTCTATGAAGGTGGTTTACAGAGGGACCTATTCCTTCCGTTTATTTCCACACTAAAGGAAAGATGTGTCGTTCGTGAGATTGGCTCATCGGTGGACTATCGGAAACTGACCTCTGCTGAAGAGGGATTTTACTTCATTGGAAAGGATATCTCAGGCCTTCTAAAACAGAAGTTTCAGCTGTTGGTTGGCGATCAACCGGCTGGTCCTCAAGTGGTTGAAGTAGTAATGGGAAGGAAATTGCAGGTTCCTCTAGCTGCTGATGGATGTGCCTACTTTCTTTTTGAAGAGCTGTGTGATAGACCCCTAGGCGCAGCCGATTATCTCGGATTATTCAGTGTACCCTTCTTCGGGCTCCATAATAGGACTGCAGCTTACCGCTTTGTGACACTGGTTGATGTAATGTATGAGAACAAGGCCAGGCTTCTATGCACGGCAGAAGGTAGCCCTCTAGAACTGCTGGAGAGAATAGTGACAATCTCTGATGCACAGCAAATAGCACCTAGAACCTCTTCAAGATCAAGGAAGAGTGATGATCCCGACCTTTGCGTGGACAACGAGCTTGGTTTTGCTAAAGACCGCACCATTAGCAGATTAACGGAGATGAACAGCAAAGAATACTTAGAACAACATTCCAGAATGTTGCAGGAGAAGCAGCCTTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

464

Amino Acids

52.21

Weight (kDa)

6.65

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 79 - 407 7.2e-88 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 961
AciI CCGC 3 cut(s) 532, 1105, 1306
AclWI GGATC 3 cut(s) 324, 554, 1256
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 2 cut(s) 251, 867
AfaI GTAC 2 cut(s) 253, 1068
AfiI CCNNNNNNNGG 4 cut(s) 401, 1076, 1077, 1276
AgsI TTSAA 6 cut(s) 293, 557, 569, 947, 1013, 1242
AjnI CCWGG 2 cut(s) 579, 1145
Alw21I GWGCWC 1 cut(s) 721
Alw26I GTCTC 5 cut(s) 153, 265, 381, 394, 668
AlwI GGATC 3 cut(s) 324, 554, 1256
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 4 cut(s) 182, 577, 883, 1143
ApeKI GCWGC 4 cut(s) 983, 1040, 1097, 1381
ApoI RAATTY 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 1237
AspA2I CCTAGG 1 cut(s) 1033
AspLEI GCGC 1 cut(s) 1040
AspS9I GGNCC 3 cut(s) 182, 754, 932
AsuNHI GCTAGC 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 2 cut(s) 754, 932
AvrII CCTAGG 1 cut(s) 1033
BanII GRGCYC 1 cut(s) 1083
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
Bbv12I GWGCWC 1 cut(s) 721
BbvI GCAGC 3 cut(s) 970, 1052, 1109
BccI CCATC 3 cut(s) 161, 224, 983
BceAI ACGGC 3 cut(s) 12, 169, 1176
BciT130I CCWGG 2 cut(s) 581, 1147
BcoDI GTCTC 5 cut(s) 153, 265, 381, 394, 668
BfaI CTAG 7 cut(s) 248, 345, 477, 980, 1034, 1178, 1230
BfmI CTRYAG 1 cut(s) 1095
BfuAI ACCTGC 1 cut(s) 961
BisI GCNGC 5 cut(s) 532, 984, 1041, 1098, 1382
BlnI CCTAGG 1 cut(s) 1033
BlsI GCNGC 5 cut(s) 533, 985, 1042, 1099, 1383
Bme1390I CCNGG 2 cut(s) 581, 1147
Bme18I GGWCC 2 cut(s) 754, 932
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 3 cut(s) 182, 754, 932
BmiI GGNNCC 3 cut(s) 755, 975, 1082
BmrFI CCNGG 2 cut(s) 581, 1147
BmsI GCATC 5 cut(s) 58, 292, 338, 634, 1201
BmtI GCTAGC 1 cut(s) 480
BplI GAGNNNNNCTC 2 cut(s) 797, 829
BpmI CTGGAG 4 cut(s) 237, 502, 705, 1208
BpuEI CTTGAG 1 cut(s) 921
BsaBI GATNNNNATC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 1033
Bsc4I CCNNNNNNNGG 4 cut(s) 401, 1076, 1077, 1276
Bse118I RCCGGY 1 cut(s) 925
Bse1I ACTGG 2 cut(s) 436, 1122
Bse8I GATNNNNATC 1 cut(s) 92
BseBI CCWGG 2 cut(s) 581, 1147
BseDI CCNNGG 1 cut(s) 1033
BseGI GGATG 2 cut(s) 235, 998
BseJI GATNNNNATC 1 cut(s) 92
BseLI CCNNNNNNNGG 4 cut(s) 401, 1076, 1077, 1276
BseMII CTCAG 1 cut(s) 894
BseNI ACTGG 2 cut(s) 436, 1122
BseRI GAGGAG 1 cut(s) 435
BseXI GCAGC 3 cut(s) 970, 1052, 1109
BseYI CCCAGC 1 cut(s) 101
BshFI GGCC 4 cut(s) 184, 579, 885, 1145
BsiHKAI GWGCWC 1 cut(s) 721
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 1 cut(s) 926
BslFI GGGAC 1 cut(s) 767
BslI CCNNNNNNNGG 4 cut(s) 401, 1076, 1077, 1276
BsmAI GTCTC 5 cut(s) 153, 265, 381, 394, 668
BsmBI CGTCTC 2 cut(s) 153, 668
BsmFI GGGAC 1 cut(s) 767
BsnI GGCC 4 cut(s) 184, 579, 885, 1145
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 721, 1083
Bsp143I GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
BspACI CCGC 3 cut(s) 532, 1105, 1306
BspANI GGCC 4 cut(s) 184, 579, 885, 1145
BspCNI CTCAG 1 cut(s) 893
BspLI GGNNCC 3 cut(s) 755, 975, 1082
BspMAI CTGCAG 1 cut(s) 1099
BspMI ACCTGC 1 cut(s) 961
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 3 cut(s) 324, 554, 1256
BspQI GCTCTTC 1 cut(s) 1008
BsrFI RCCGGY 1 cut(s) 925
BsrI ACTGG 2 cut(s) 436, 1122
BssAI RCCGGY 1 cut(s) 925
BssECI CCNNGG 1 cut(s) 1033
BssMI GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
BssT1I CCWWGG 1 cut(s) 1033
Bst2UI CCWGG 2 cut(s) 581, 1147
Bst4CI ACNGT 2 cut(s) 662, 716
Bst6I CTCTTC 5 cut(s) 388, 843, 1008, 1243, 1247
BstC8I GCNNGC 3 cut(s) 474, 478, 536
BstDEI CTNAG 5 cut(s) 257, 613, 880, 1348, 1392
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 2 cut(s) 235, 998
BstHHI GCGC 1 cut(s) 1040
BstKTI GATC 6 cut(s) 90, 319, 562, 922, 1247, 1264
BstMAI GTCTC 5 cut(s) 153, 265, 381, 394, 668
BstMBI GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 596, 1314, 1378
BstNI CCWGG 2 cut(s) 581, 1147
BstSCI CCNGG 2 cut(s) 579, 1145
BstSFI CTRYAG 1 cut(s) 1095
BstV1I GCAGC 3 cut(s) 970, 1052, 1109
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 480
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 4 cut(s) 184, 579, 885, 1145
BtsCI GGATG 2 cut(s) 235, 998
BtsIMutI CAGTG 3 cut(s) 647, 1069, 1115
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 3 cut(s) 474, 478, 536
CfoI GCGC 1 cut(s) 1040
Cfr10I RCCGGY 1 cut(s) 925
Cfr13I GGNCC 3 cut(s) 182, 754, 932
Csp6I GTAC 2 cut(s) 252, 1067
CviAII CATG 3 cut(s) 448, 514, 625
CviQI GTAC 2 cut(s) 252, 1067
DdeI CTNAG 5 cut(s) 257, 613, 880, 1348, 1392
DpnI GATC 6 cut(s) 89, 318, 561, 921, 1246, 1263
DpnII GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
Eam1104I CTCTTC 5 cut(s) 388, 843, 1008, 1243, 1247
EarI CTCTTC 5 cut(s) 388, 843, 1008, 1243, 1247
Eco130I CCWWGG 1 cut(s) 1033
Eco147I AGGCCT 1 cut(s) 885
Eco24I GRGCYC 1 cut(s) 1083
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 2 cut(s) 754, 932
Eco57I CTGAAG 2 cut(s) 251, 867
Eco88I CYCGRG 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 754
EcoRII CCWGG 2 cut(s) 579, 1145
EcoRV GATATC 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 1033
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 1033
Esp3I CGTCTC 2 cut(s) 153, 668
FaeI CATG 3 cut(s) 451, 517, 628
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FaqI GGGAC 1 cut(s) 767
FatI CATG 3 cut(s) 447, 513, 624
Fnu4HI GCNGC 5 cut(s) 532, 984, 1041, 1098, 1382
FokI GGATG 2 cut(s) 242, 1005
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 5 cut(s) 532, 984, 1041, 1098, 1382
FspBI CTAG 7 cut(s) 248, 345, 477, 980, 1034, 1178, 1230
GlaI GCGC 1 cut(s) 1039
GluI GCNGC 5 cut(s) 532, 984, 1041, 1098, 1382
GsaI CCCAGC 1 cut(s) 105
GsuI CTGGAG 4 cut(s) 237, 502, 705, 1208
HaeIII GGCC 4 cut(s) 184, 579, 885, 1145
HapII CCGG 1 cut(s) 926
HhaI GCGC 1 cut(s) 1040
Hin1II CATG 3 cut(s) 451, 517, 628
Hin6I GCGC 1 cut(s) 1038
HinP1I GCGC 1 cut(s) 1038
HinfI GANTC 3 cut(s) 207, 273, 496
HpaII CCGG 1 cut(s) 926
Hpy166II GTNNAC 6 cut(s) 254, 342, 746, 823, 1067, 1279
Hpy188I TCNGA 4 cut(s) 270, 831, 1055, 1210
Hpy188III TCNNGA 9 cut(s) 43, 361, 554, 722, 803, 1178, 1242, 1265, 1362
Hpy8I GTNNAC 6 cut(s) 254, 342, 746, 823, 1067, 1279
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 9 cut(s) 32, 148, 731, 773, 896, 954, 1081, 1160, 1395
HpyCH4III ACNGT 2 cut(s) 662, 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 1314, 1378
HpyF3I CTNAG 5 cut(s) 257, 613, 880, 1348, 1392
Hsp92II CATG 3 cut(s) 451, 517, 628
HspAI GCGC 1 cut(s) 1038
Kzo9I GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
LguI GCTCTTC 1 cut(s) 1008
LmnI GCTCC 3 cut(s) 73, 724, 1086
Lsp1109I GCAGC 3 cut(s) 970, 1052, 1109
LweI GCATC 5 cut(s) 58, 292, 338, 634, 1201
MaeI CTAG 7 cut(s) 248, 345, 477, 980, 1034, 1178, 1230
MaeIII GTNAC 3 cut(s) 31, 1111, 1198
MalI GATC 6 cut(s) 89, 318, 561, 921, 1246, 1263
MboI GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
MfeI CAATTG 1 cut(s) 467
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 721, 1083
MluCI AATT 6 cut(s) 79, 352, 368, 467, 508, 965
MlyI GAGTC 1 cut(s) 267
MroXI GAANNNNTTC 1 cut(s) 1237
MseI TTAA 2 cut(s) 681, 1322
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 907
MspI CCGG 1 cut(s) 926
MspR9I CCNGG 2 cut(s) 581, 1147
MunI CAATTG 1 cut(s) 467
MvaI CCWGG 2 cut(s) 581, 1147
MwoI GCNNNNNNNGC 3 cut(s) 596, 1314, 1378
NdeII GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 3 cut(s) 451, 517, 628
NlaIV GGNNCC 3 cut(s) 755, 975, 1082
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 2 cut(s) 1111, 1198
PaeR7I CTCGAG 1 cut(s) 43
PceI AGGCCT 1 cut(s) 885
PciSI GCTCTTC 1 cut(s) 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 1237
PfeI GAWTC 2 cut(s) 207, 496
PkrI GCNGC 5 cut(s) 533, 985, 1042, 1099, 1383
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
PpuMI RGGWCCY 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 754
Psp6I CCWGG 2 cut(s) 579, 1145
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 2 cut(s) 579, 1145
PspN4I GGNNCC 3 cut(s) 755, 975, 1082
PspPI GGNCC 3 cut(s) 182, 754, 932
PspPPI RGGWCCY 1 cut(s) 754
PstI CTGCAG 1 cut(s) 1099
PsuI RGATCY 1 cut(s) 559
PvuII CAGCTG 1 cut(s) 907
RsaI GTAC 2 cut(s) 253, 1068
RsaNI GTAC 2 cut(s) 252, 1067
RseI CAYNNNNRTG 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 1008
SaqAI TTAA 2 cut(s) 681, 1322
SatI GCNGC 5 cut(s) 532, 984, 1041, 1098, 1382
Sau3AI GATC 6 cut(s) 87, 316, 559, 919, 1244, 1261
Sau96I GGNCC 3 cut(s) 182, 754, 932
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 581, 1147
SduI GDGCHC 2 cut(s) 721, 1083
SfaNI GCATC 5 cut(s) 58, 292, 338, 634, 1201
SfcI CTRYAG 1 cut(s) 1095
Sfr274I CTCGAG 1 cut(s) 43
SinI GGWCC 2 cut(s) 754, 932
SlaI CTCGAG 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 43, 936
SmoI CTYRAG 2 cut(s) 43, 936
Sse9I AATT 6 cut(s) 79, 352, 368, 467, 508, 965
SseBI AGGCCT 1 cut(s) 885
SsiI CCGC 3 cut(s) 532, 1105, 1306
SspMI CTAG 7 cut(s) 248, 345, 477, 980, 1034, 1178, 1230
StuI AGGCCT 1 cut(s) 885
StyD4I CCNGG 2 cut(s) 579, 1145
StyI CCWWGG 1 cut(s) 1033
TaaI ACNGT 2 cut(s) 662, 716
TaqI TCGA 2 cut(s) 44, 310
TasI AATT 6 cut(s) 79, 352, 368, 467, 508, 965
TauI GCSGC 1 cut(s) 534
TfiI GAWTC 2 cut(s) 207, 496
Tru1I TTAA 2 cut(s) 681, 1322
Tru9I TTAA 2 cut(s) 681, 1322
TscAI CASTG 3 cut(s) 654, 1069, 1122
TseFI GTSAC 2 cut(s) 1111, 1198
TseI GCWGC 4 cut(s) 983, 1040, 1097, 1381
Tsp45I GTSAC 2 cut(s) 1111, 1198
TspDTI ATGAA 9 cut(s) 65, 105, 213, 452, 502, 613, 750, 853, 1346
TspGWI ACGGA 2 cut(s) 756, 1340
TspRI CASTG 3 cut(s) 654, 1069, 1122
VpaK11BI GGWCC 2 cut(s) 754, 932
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 1 cut(s) 368
XbaI TCTAGA 1 cut(s) 1177
XhoI CTCGAG 1 cut(s) 43
XmaJI CCTAGG 1 cut(s) 1033
XmnI GAANNNNTTC 1 cut(s) 1237
XspI CTAG 7 cut(s) 248, 345, 477, 980, 1034, 1178, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.