Rroxscaffold_2G00108990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001671
Physical Location & Seq
Reverse (-)
33067258 .. 33067857
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108990.1

Sequence Viewer

Length: 600 bp
ATGACACTCAGAAGCCGTTCTTGTGCGGTGACCACCCATCTTAGCCCTAGGTTTCTCATCATCTCTTGCCGAAAGGAAATAGGTGATGTGAGGGATATGAATCAGTGTTCCAGTCCAATCAGATTCAATGGTGATTGGGTGGACGAATGGCCGTGGTGGATCAGGCCGAGGCGAGGTAGGCTCAAGGCTAGGTTGTACGCAGTCGGGAATGATCGGTGCCTACAGGCTGGAAGCCAAGAGTGGAGAGGTATTGTGTGGCGGACGTGCGACTTCTGGACCCGGATCAAAGCGACCAAATTTAGATTGATAGCGGCGAGAGCCTTTGCACACCAACCCGTGGATGTGGAGGAGATAGTCGCTGTGCACCGTGCAGCGAACCAGCATCTGGAGCATGCAGAGCTCGCCAGTGGGTTGTCTGAGTCGGTGATGGTGGCTACATCTGGCATCCGGATTGGCAAGGGAGTGGAAACGGCGGCGTCTGGCGTACTTGGCAAAGGAGCGGCAAGTGGGGTGTCCAGAACATTTTGGGGGTCCGAATGGATTGGGTGCCTAAATCATTTTGGAAGTCCAAAAGGCTGTGGTGGCCCAAATCAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

22.09

Weight (kDa)

9.76

Isoelectric Point (pI)

31.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 216, 546
AccB7I CCANNNNNTGG 2 cut(s) 337, 385
AccBSI CCGCTC 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 5 cut(s) 26, 259, 311, 473, 500
AclWI GGATC 2 cut(s) 167, 290
AcoI YGGCCR 1 cut(s) 149
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 1 cut(s) 476
AfaI GTAC 2 cut(s) 197, 486
AfiI CCNNNNNNNGG 3 cut(s) 173, 337, 385
AgsI TTSAA 1 cut(s) 127
AjiI CACGTC 1 cut(s) 264
AluBI AGCT 2 cut(s) 400, 595
AluI AGCT 2 cut(s) 400, 595
Alw21I GWGCWC 2 cut(s) 366, 402
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 2 cut(s) 167, 290
AlwNI CAGNNNCTG 1 cut(s) 385
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 3 cut(s) 149, 164, 583
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 1 cut(s) 371
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 16
AspA2I CCTAGG 1 cut(s) 47
AspS9I GGNCC 3 cut(s) 276, 531, 584
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 4 cut(s) 40, 95, 143, 436
AvaII GGWCC 2 cut(s) 276, 531
AvrII CCTAGG 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 216, 546
BanII GRGCYC 1 cut(s) 402
Bbv12I GWGCWC 2 cut(s) 366, 402
BbvI GCAGC 1 cut(s) 383
BccI CCATC 2 cut(s) 45, 421
BceAI ACGGC 2 cut(s) 136, 486
BcnI CCSGG 1 cut(s) 280
BfaI CTAG 2 cut(s) 48, 189
BfmI CTRYAG 1 cut(s) 221
BisI GCNGC 4 cut(s) 312, 372, 474, 501
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 4 cut(s) 313, 373, 475, 502
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 2 cut(s) 276, 531
BmgBI CACGTC 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 276, 531, 584
BmiI GGNNCC 4 cut(s) 218, 278, 532, 548
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 391, 453
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 280
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 47, 152, 167, 336
BsaWI WCCGGW 1 cut(s) 447
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 337, 385
Bse1I ACTGG 2 cut(s) 111, 405
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 447
BseDI CCNNGG 4 cut(s) 47, 152, 167, 336
BseGI GGATG 2 cut(s) 346, 444
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 173, 337, 385
BseMII CTCAG 2 cut(s) 22, 408
BseNI ACTGG 2 cut(s) 111, 405
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 3 cut(s) 151, 166, 585
BshNI GGYRCC 2 cut(s) 216, 546
BsiHKAI GWGCWC 2 cut(s) 366, 402
BsiSI CCGG 2 cut(s) 280, 448
BslI CCNNNNNNNGG 3 cut(s) 173, 337, 385
BsnI GGCC 3 cut(s) 151, 166, 585
Bsp1286I GDGCHC 2 cut(s) 366, 402
Bsp13I TCCGGA 1 cut(s) 447
Bsp143I GATC 3 cut(s) 159, 211, 282
BspACI CCGC 5 cut(s) 26, 259, 311, 473, 500
BspANI GGCC 3 cut(s) 151, 166, 585
BspCNI CTCAG 2 cut(s) 21, 409
BspEI TCCGGA 1 cut(s) 447
BspLI GGNNCC 4 cut(s) 218, 278, 532, 548
BspPI GGATC 2 cut(s) 167, 290
BspT107I GGYRCC 2 cut(s) 216, 546
BsrBI CCGCTC 1 cut(s) 500
BsrI ACTGG 2 cut(s) 111, 405
BssECI CCNNGG 4 cut(s) 47, 152, 167, 336
BssMI GATC 3 cut(s) 159, 211, 282
BssNI GRCGYC 1 cut(s) 476
BssT1I CCWWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 368
BstACI GRCGYC 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 393, 402
BstDEI CTNAG 3 cut(s) 8, 41, 417
BstDSI CCRYGG 2 cut(s) 152, 336
BstEII GGTNACC 1 cut(s) 28
BstF5I GGATG 2 cut(s) 346, 444
BstKTI GATC 3 cut(s) 162, 214, 285
BstMBI GATC 3 cut(s) 159, 211, 282
BstMWI GCNNNNNNNGC 7 cut(s) 178, 317, 388, 397, 401, 489, 582
BstNSI RCATGY 1 cut(s) 395
BstPI GGTNACC 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 221
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 1 cut(s) 383
BsuRI GGCC 3 cut(s) 151, 166, 585
BtgI CCRYGG 2 cut(s) 152, 336
BtrI CACGTC 1 cut(s) 264
BtsCI GGATG 2 cut(s) 346, 444
BtsIMutI CAGTG 2 cut(s) 110, 412
Cac8I GCNNGC 2 cut(s) 393, 402
CaiI CAGNNNCTG 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 276, 531, 584
CseI GACGC 1 cut(s) 465
Csp6I GTAC 2 cut(s) 196, 485
CviAII CATG 1 cut(s) 392
CviQI GTAC 2 cut(s) 196, 485
DdeI CTNAG 3 cut(s) 8, 41, 417
DpnI GATC 3 cut(s) 161, 213, 284
DpnII GATC 3 cut(s) 159, 211, 282
EaeI YGGCCR 1 cut(s) 149
EciI GGCGGA 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 402
Eco47I GGWCC 2 cut(s) 276, 531
Eco53kI GAGCTC 1 cut(s) 400
Eco91I GGTNACC 1 cut(s) 28
EcoICRI GAGCTC 1 cut(s) 400
EcoO65I GGTNACC 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 1 cut(s) 395
FaiI YATR 2 cut(s) 98, 393
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 1 cut(s) 391
Fnu4HI GCNGC 4 cut(s) 312, 372, 474, 501
FokI GGATG 2 cut(s) 353, 431
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 4 cut(s) 312, 372, 474, 501
FspBI CTAG 2 cut(s) 48, 189
GluI GCNGC 4 cut(s) 312, 372, 474, 501
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 3 cut(s) 151, 166, 585
HapII CCGG 2 cut(s) 280, 448
HgaI GACGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 1 cut(s) 395
HinfI GANTC 3 cut(s) 100, 123, 419
HpaII CCGG 2 cut(s) 280, 448
HphI GGTGA 4 cut(s) 40, 95, 143, 436
Hpy166II GTNNAC 2 cut(s) 142, 364
Hpy188I TCNGA 4 cut(s) 11, 122, 418, 535
Hpy188III TCNNGA 5 cut(s) 205, 274, 386, 448, 516
Hpy8I GTNNAC 2 cut(s) 142, 364
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 4 cut(s) 326, 364, 371, 395
HpyF10VI GCNNNNNNNGC 7 cut(s) 178, 317, 388, 397, 401, 489, 582
HpyF3I CTNAG 3 cut(s) 8, 41, 417
HpySE526I ACGT 1 cut(s) 263
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 1 cut(s) 395
Kpn2I TCCGGA 1 cut(s) 447
Kzo9I GATC 3 cut(s) 159, 211, 282
LmnI GCTCC 2 cut(s) 388, 497
Lsp1109I GCAGC 1 cut(s) 383
LweI GCATC 2 cut(s) 391, 453
MaeI CTAG 2 cut(s) 48, 189
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 161, 213, 284
MbiI CCGCTC 1 cut(s) 500
MboI GATC 3 cut(s) 159, 211, 282
MhlI GDGCHC 2 cut(s) 366, 402
MluCI AATT 1 cut(s) 296
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 5 cut(s) 84, 162, 167, 239, 340
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 1 cut(s) 598
MspI CCGG 2 cut(s) 280, 448
MspR9I CCNGG 1 cut(s) 280
MwoI GCNNNNNNNGC 7 cut(s) 178, 317, 388, 397, 401, 489, 582
NciI CCSGG 1 cut(s) 280
NdeII GATC 3 cut(s) 159, 211, 282
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 4 cut(s) 218, 278, 532, 548
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 28
NspI RCATGY 1 cut(s) 395
PaeI GCATGC 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 2 cut(s) 100, 123
PflMI CCANNNNNTGG 2 cut(s) 337, 385
PkrI GCNGC 4 cut(s) 313, 373, 475, 502
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 402
PspEI GGTNACC 1 cut(s) 28
PspN4I GGNNCC 4 cut(s) 218, 278, 532, 548
PspPI GGNCC 3 cut(s) 276, 531, 584
PstNI CAGNNNCTG 1 cut(s) 385
RsaI GTAC 2 cut(s) 197, 486
RsaNI GTAC 2 cut(s) 196, 485
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 1 cut(s) 598
SatI GCNGC 4 cut(s) 312, 372, 474, 501
Sau3AI GATC 3 cut(s) 159, 211, 282
Sau96I GGNCC 3 cut(s) 276, 531, 584
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 366, 402
SetI ASST 8 cut(s) 53, 85, 178, 194, 250, 266, 402, 597
SfaNI GCATC 2 cut(s) 391, 453
SfcI CTRYAG 1 cut(s) 221
SinI GGWCC 2 cut(s) 276, 531
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SphI GCATGC 1 cut(s) 395
Sse9I AATT 1 cut(s) 296
SsiI CCGC 5 cut(s) 26, 259, 311, 473, 500
SspMI CTAG 2 cut(s) 48, 189
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 1 cut(s) 266
TasI AATT 1 cut(s) 296
TauI GCSGC 3 cut(s) 314, 476, 503
TfiI GAWTC 2 cut(s) 100, 123
Tru1I TTAA 1 cut(s) 598
Tru9I TTAA 1 cut(s) 598
TscAI CASTG 2 cut(s) 110, 412
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 2 cut(s) 110, 412
Van91I CCANNNNNTGG 2 cut(s) 337, 385
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 276, 531
XapI RAATTY 1 cut(s) 296
XceI RCATGY 1 cut(s) 395
XmaJI CCTAGG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 2 cut(s) 48, 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.