Rroxscaffold_1G00033980

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001671
Physical Location & Seq
Forward (+)
49116236 .. 49117689
1454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00033980.1

Sequence Viewer

Length: 693 bp
ATGGCGTCGGCGTCGGCGTCGTCTTCGGCTCTCTCAAGACTGTCCGGTATCGGCGGACACTTCAATCCCTCCCACGCAAACAAGGACGGTGTGGCGTGTGATGATGATGATAAGACCCTCTTGAAGTTGTTGCGGGAGAGATATCCCGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTACCGATGCATTATTACTAAATCGTCTATTTAGACATCTATTCGACAATGGCATTATTCTTGTTGCCACCTCCAATCGTGCTCCGGATAACCCGTATGAACCGAGAGACTACCGAAGACCGACTTCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCACGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCCGAGGAGGCGAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTACGAGAACAAGGGCAGATCGATGTGTACAGGCGAGGGGACTCCATTTGAACTTTTTGAAAGAATAGTGACAATCCCCGATGCCCAACAAGTGGCACCTAGAACCTCTTCGAGATCAAGGAAAAATGATGATTCGGAGCTATGTGTAGATAATGAATTGGGGTTTGCTAAAGACCGCACCATTAGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

230

Amino Acids

25.82

Weight (kDa)

5.5

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 40 - 99 4.2e-18 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 595
AccB7I CCANNNNNTGG 1 cut(s) 592
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 3 cut(s) 54, 133, 676
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 3 cut(s) 5, 11, 17
AfaI GTAC 2 cut(s) 503, 529
AfiI CCNNNNNNNGG 2 cut(s) 592, 687
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 5 cut(s) 64, 124, 383, 551, 560
AluBI AGCT 2 cut(s) 407, 640
AluI AGCT 2 cut(s) 407, 640
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 1 cut(s) 294
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 176
Asp700I GAANNNNTTC 2 cut(s) 378, 607
AsuHPI GGTGA 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 413
BanI GGYRCC 1 cut(s) 595
BbsI GAAGAC 2 cut(s) 15, 316
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 294
BfaI CTAG 2 cut(s) 459, 600
BfrI CTTAAG 1 cut(s) 368
BisI GCNGC 1 cut(s) 468
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 413
BmiI GGNNCC 2 cut(s) 454, 597
BmsI GCATC 2 cut(s) 190, 571
BpiI GAAGAC 2 cut(s) 15, 316
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 19
Bsa29I ATCGAT 1 cut(s) 521
BsaHI GRCGYC 3 cut(s) 5, 11, 17
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 2 cut(s) 44, 277
Bsc4I CCNNNNNNNGG 2 cut(s) 592, 687
BseAI TCCGGA 1 cut(s) 277
BseCI ATCGAT 1 cut(s) 521
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 2 cut(s) 592, 687
BseMII CTCAG 1 cut(s) 315
BseRI GAGGAG 1 cut(s) 431
BseXI GCAGC 1 cut(s) 479
BshNI GGYRCC 1 cut(s) 595
BshVI ATCGAT 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 2 cut(s) 45, 278
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 2 cut(s) 592, 687
BsmAI GTCTC 1 cut(s) 294
BsmFI GGGAC 1 cut(s) 553
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 277
Bsp13I TCCGGA 1 cut(s) 277
Bsp1407I TGTACA 1 cut(s) 527
Bsp143I GATC 2 cut(s) 518, 614
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 3 cut(s) 54, 133, 676
BspCNI CTCAG 1 cut(s) 316
BspDI ATCGAT 1 cut(s) 521
BspEI TCCGGA 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 454, 597
BspT107I GGYRCC 1 cut(s) 595
BspTI CTTAAG 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 518, 614
BssNI GRCGYC 3 cut(s) 5, 11, 17
Bst4CI ACNGT 2 cut(s) 42, 89
Bst6I CTCTTC 1 cut(s) 613
BstACI GRCGYC 3 cut(s) 5, 11, 17
BstAFI CTTAAG 1 cut(s) 368
BstAUI TGTACA 1 cut(s) 527
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 2 cut(s) 521, 617
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 518, 614
BstV1I GCAGC 1 cut(s) 479
BstV2I GAAGAC 2 cut(s) 15, 316
Bsu15I ATCGAT 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 521
BtsCI GGATG 1 cut(s) 409
ClaI ATCGAT 1 cut(s) 521
CseI GACGC 1 cut(s) 6
Csp6I GTAC 2 cut(s) 502, 528
CviAII CATG 1 cut(s) 181
CviJI RGCY 4 cut(s) 29, 323, 407, 640
CviKI_1 RGCY 4 cut(s) 29, 323, 407, 640
CviQI GTAC 2 cut(s) 502, 528
DdeI CTNAG 1 cut(s) 324
DpnI GATC 2 cut(s) 520, 616
DpnII GATC 2 cut(s) 518, 614
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
EciI GGCGGA 1 cut(s) 69
Eco32I GATATC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 176
EcoRV GATATC 1 cut(s) 143
EcoT22I ATGCAT 1 cut(s) 205
FaeI CATG 1 cut(s) 184
FaiI YATR 6 cut(s) 166, 182, 291, 435, 472, 643
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 301, 333
FaqI GGGAC 1 cut(s) 553
FatI CATG 1 cut(s) 180
FauI CCCGC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 2 cut(s) 459, 600
GluI GCNGC 1 cut(s) 468
HapII CCGG 2 cut(s) 45, 278
HgaI GACGC 1 cut(s) 6
Hin1I GRCGYC 3 cut(s) 5, 11, 17
Hin1II CATG 1 cut(s) 184
HinfI GANTC 2 cut(s) 541, 632
HpaII CCGG 2 cut(s) 45, 278
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 1 cut(s) 637
Hpy188III TCNNGA 8 cut(s) 36, 121, 146, 170, 278, 383, 413, 612
Hpy8I GTNNAC 1 cut(s) 528
Hpy99I CGWCG 3 cut(s) 10, 16, 22
HpyCH4III ACNGT 2 cut(s) 42, 89
HpyCH4V TGCA 1 cut(s) 203
HpyF3I CTNAG 1 cut(s) 324
Hsp92I GRCGYC 3 cut(s) 5, 11, 17
Hsp92II CATG 1 cut(s) 184
Kpn2I TCCGGA 1 cut(s) 277
Kzo9I GATC 2 cut(s) 518, 614
LmnI GCTCC 2 cut(s) 280, 637
LpnPI CCDG 3 cut(s) 58, 291, 516
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 2 cut(s) 190, 571
MaeI CTAG 2 cut(s) 459, 600
MaeIII GTNAC 5 cut(s) 184, 193, 357, 481, 568
MalI GATC 2 cut(s) 520, 616
MboI GATC 2 cut(s) 518, 614
MboII GAAGA 4 cut(s) 15, 321, 451, 600
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 3 cut(s) 176, 386, 656
MlyI GAGTC 1 cut(s) 535
MnlI CCTC 8 cut(s) 79, 128, 145, 274, 409, 412, 529, 616
Mph1103I ATGCAT 1 cut(s) 205
MroI TCCGGA 1 cut(s) 277
MroXI GAANNNNTTC 2 cut(s) 378, 607
MseI TTAA 2 cut(s) 369, 447
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 45, 278
NdeII GATC 2 cut(s) 518, 614
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 2 cut(s) 454, 597
NmuCI GTSAC 3 cut(s) 184, 357, 568
NsiI ATGCAT 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 2 cut(s) 378, 607
PfeI GAWTC 1 cut(s) 632
PflMI CCANNNNNTGG 1 cut(s) 592
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
PspN4I GGNNCC 2 cut(s) 454, 597
RsaI GTAC 2 cut(s) 503, 529
RsaNI GTAC 2 cut(s) 502, 528
SaqAI TTAA 2 cut(s) 369, 447
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 2 cut(s) 518, 614
SchI GAGTC 1 cut(s) 535
SduI GDGCHC 1 cut(s) 277
SetI ASST 7 cut(s) 266, 409, 454, 601, 608, 642, 692
SfaNI GCATC 2 cut(s) 190, 571
SmlI CTYRAG 2 cut(s) 34, 368
SmoI CTYRAG 2 cut(s) 34, 368
Sse9I AATT 3 cut(s) 176, 386, 656
SsiI CCGC 3 cut(s) 54, 133, 676
SspMI CTAG 2 cut(s) 459, 600
TaaI ACNGT 2 cut(s) 42, 89
TaqI TCGA 3 cut(s) 237, 521, 611
TaqII GACCGA 1 cut(s) 328
TasI AATT 3 cut(s) 176, 386, 656
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 1 cut(s) 632
Tru1I TTAA 2 cut(s) 369, 447
Tru9I TTAA 2 cut(s) 369, 447
TseFI GTSAC 3 cut(s) 184, 357, 568
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 3 cut(s) 184, 357, 568
TspDTI ATGAA 4 cut(s) 169, 189, 306, 669
Van91I CCANNNNNTGG 1 cut(s) 592
Vha464I CTTAAG 1 cut(s) 368
XapI RAATTY 1 cut(s) 176
XmnI GAANNNNTTC 2 cut(s) 378, 607
XspI CTAG 2 cut(s) 459, 600
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.