FvH4_4g08720

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001671
Physical Location & Seq
Reverse (-)
9272884 .. 9278601
5718 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08720.t2

Sequence Viewer

Length: 1503 bp
ATGAGAGCTGTTGCTCGTTCTGTCCATCAATTTCGATCAGCTTTGCGGCAGCAGACATGTAGTTACTCCACCGGTGTTGTGAGAAGGCGAAAGCTTTTGACAAACAGCTATTTTGCGTTAGCTCATGAGCTCCCTAACAATGCCAAAGTCGATAGATTTATGCATCCCGCCATTTTTGGCTCTAGAGCTCTGTCATTCAATGCTGCTCCGGTTACTAATGGAGACACACACAAAGGAGGGCCGCTTGTGGAGTATGAGCGAAGAATTGCTGCGGGTGAACTTGTTGATGGTGATGCCTGCCAGGTAGGCACCTTAAGAGAACTGCAAAGGCTATATGATCAACTTGTTGAGTCGGCTGATGTCTGCCAGTTGGATAGATATGCCACTTCGAGGAAATCTGCAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAATCTTCAAACGCCTCCCCTGTCAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGAACAACTACAATGCAGTTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAGGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAATCTTGTTATGTCTCGATGAATTCATGGTGAATGATGTTGCCGATGCGTTAATACTAAATCGTCTGTTTAGACACCTATTCAACAATGGTGTTATTCTTGTCGCCACCTCCAATCGTGCTCCTGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTCATTGCTGCTTTAAAGGAAAGATGTGTAGTTCATGAAATTGGTTCTGCGGTAGACTACCGAAAACTCACATCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAGGATTTGTCAAGCTTTCTCCAGGGGAAGTTTCAAGAATTGATTGGGGATCATGAAGCTCATCCTGAGGAGGCAGAAGTAGTGATGGGAAGAACACTAAAGGTTCCACTAGGTGCCAATGGATGCGCTTACTTTCCTTTTGAGGAACTTTGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCCTGGCCTTGGACGGTGTTCCTATCTTCGGACCCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTATGAAAACAAGGGCAGACTGATGTGTACAGCTGAGGGGACTCCTTTTGAACTTTTTGAAAGAATAGTGACAATCCAACAAGTGGCACCTAGAACCTCTTCAAGATCAAGGAAAAATGATGATTTGGAGTTATGTGTAGATAATGAATTGGGATTTGCTAAAGACCGCACCATTAGTAGATTAACAGAGATGAATAGCAAAGAATACTTGGAGCATCATGCTGCAGCGCTGGAAGAGAACAACTCCCATGAAGATCTGCATCTGGATAAAACCGTGCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

501

Amino Acids

56.55

Weight (kDa)

6.56

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 82 - 427 1.4e-89 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 308, 1043, 1303
AccB7I CCANNNNNTGG 2 cut(s) 1138, 1300
AccI GTMKAC 1 cut(s) 882
AciI CCGC 6 cut(s) 46, 168, 242, 272, 878, 1384
AclWI GGATC 2 cut(s) 822, 987
AcsI RAATTY 1 cut(s) 680
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 1 cut(s) 1246
AfeI AGCGCT 1 cut(s) 1446
AfiI CCNNNNNNNGG 7 cut(s) 390, 461, 1138, 1144, 1163, 1176, 1300
AflII CTTAAG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 56
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 9 cut(s) 199, 444, 580, 629, 742, 965, 1268, 1277, 1320
AjnI CCWGG 4 cut(s) 300, 606, 951, 1137
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 3 cut(s) 132, 190, 781
Alw26I GTCTC 3 cut(s) 216, 426, 677
AlwI GGATC 2 cut(s) 822, 987
Aor51HI AGCGCT 1 cut(s) 1446
AoxI GGCC 2 cut(s) 239, 1140
ApeKI GCWGC 9 cut(s) 49, 203, 269, 591, 836, 1100, 1184, 1439, 1442
ApoI RAATTY 1 cut(s) 680
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AsiGI ACCGGT 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 1315
AspLEI GCGC 2 cut(s) 1058, 1447
AspS9I GGNCC 3 cut(s) 239, 619, 1166
AsuHPI GGTGA 3 cut(s) 287, 302, 700
AsuII TTCGAA 1 cut(s) 522
AvaII GGWCC 2 cut(s) 619, 1166
AxyI CCTNAGG 1 cut(s) 996
BanI GGYRCC 3 cut(s) 308, 1043, 1303
BanII GRGCYC 2 cut(s) 132, 190
Bbv12I GWGCWC 3 cut(s) 132, 190, 781
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 9 cut(s) 61, 190, 256, 578, 823, 1087, 1196, 1426, 1454
BccI CCATC 5 cut(s) 33, 281, 405, 502, 1009
BciT130I CCWGG 4 cut(s) 302, 608, 953, 1139
BclI TGATCA 1 cut(s) 337
BcoDI GTCTC 3 cut(s) 216, 426, 677
BfaI CTAG 3 cut(s) 183, 1040, 1308
BfmI CTRYAG 2 cut(s) 1101, 1440
BfoI RGCGCY 1 cut(s) 1448
BfrI CTTAAG 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 306
BglII AGATCT 1 cut(s) 1471
BlpI GCTNAGC 1 cut(s) 906
Bme1390I CCNGG 4 cut(s) 302, 608, 953, 1139
Bme18I GGWCC 2 cut(s) 619, 1166
BmgT120I GGNCC 3 cut(s) 239, 619, 1166
BmiI GGNNCC 6 cut(s) 310, 621, 1035, 1045, 1168, 1305
BmrFI CCNGG 4 cut(s) 302, 608, 953, 1139
BmsI GCATC 6 cut(s) 172, 283, 694, 1043, 1441, 1486
BplI GAGNNNNNCTC 2 cut(s) 1445, 1477
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu1102I GCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 1476
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 607, 952, 1137, 1143
BsaWI WCCGGW 2 cut(s) 71, 208
Bsc4I CCNNNNNNNGG 7 cut(s) 390, 461, 1138, 1144, 1163, 1176, 1300
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 367, 496
Bse21I CCTNAGG 1 cut(s) 996
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 1476
BseBI CCWGG 4 cut(s) 302, 608, 953, 1139
BseDI CCNNGG 4 cut(s) 607, 952, 1137, 1143
BseGI GGATG 4 cut(s) 163, 429, 991, 1058
BseJI GATNNNNATC 1 cut(s) 1476
BseLI CCNNNNNNNGG 7 cut(s) 390, 461, 1138, 1144, 1163, 1176, 1300
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 3 cut(s) 897, 987, 1242
BseNI ACTGG 2 cut(s) 367, 496
BseRI GAGGAG 2 cut(s) 495, 1013
BseXI GCAGC 9 cut(s) 61, 190, 256, 578, 823, 1087, 1196, 1426, 1454
BshFI GGCC 2 cut(s) 241, 1142
BshNI GGYRCC 3 cut(s) 308, 1043, 1303
BshTI ACCGGT 1 cut(s) 71
BsiHKAI GWGCWC 3 cut(s) 132, 190, 781
BsiSI CCGG 2 cut(s) 72, 209
BslFI GGGAC 1 cut(s) 1270
BslI CCNNNNNNNGG 7 cut(s) 390, 461, 1138, 1144, 1163, 1176, 1300
BsmAI GTCTC 3 cut(s) 216, 426, 677
BsmFI GGGAC 1 cut(s) 1270
BsnI GGCC 2 cut(s) 241, 1142
Bso31I GGTCTC 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 522
Bsp1286I GDGCHC 3 cut(s) 132, 190, 781
Bsp1407I TGTACA 1 cut(s) 1244
Bsp143I GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
Bsp1720I GCTNAGC 1 cut(s) 906
BspACI CCGC 6 cut(s) 46, 168, 242, 272, 878, 1384
BspANI GGCC 2 cut(s) 241, 1142
BspCNI CTCAG 3 cut(s) 898, 988, 1243
BspHI TCATGA 4 cut(s) 124, 565, 862, 982
BspLI GGNNCC 6 cut(s) 310, 621, 1035, 1045, 1168, 1305
BspMAI CTGCAG 2 cut(s) 1105, 1444
BspPI GGATC 2 cut(s) 822, 987
BspT104I TTCGAA 1 cut(s) 522
BspT107I GGYRCC 3 cut(s) 308, 1043, 1303
BspTI CTTAAG 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 426
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 1 cut(s) 71
BsrGI TGTACA 1 cut(s) 1244
BsrI ACTGG 2 cut(s) 367, 496
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 4 cut(s) 607, 952, 1137, 1143
BssMI GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
BssT1I CCWWGG 1 cut(s) 1143
Bst2UI CCWGG 4 cut(s) 302, 608, 953, 1139
Bst4CI ACNGT 2 cut(s) 1151, 1492
Bst6I CTCTTC 2 cut(s) 1321, 1446
BstAFI CTTAAG 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 1244
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 298, 596, 1497
BstDEI CTNAG 3 cut(s) 906, 996, 1251
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 163, 429, 991, 1058
BstH2I RGCGCY 1 cut(s) 1448
BstHHI GCGC 2 cut(s) 1058, 1447
BstKTI GATC 6 cut(s) 38, 340, 817, 982, 1325, 1474
BstMAI GTCTC 3 cut(s) 216, 426, 677
BstMBI GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 4 cut(s) 302, 608, 953, 1139
BstNSI RCATGY 2 cut(s) 60, 1499
BstSCI CCNGG 4 cut(s) 300, 606, 951, 1137
BstSFI CTRYAG 2 cut(s) 1101, 1440
BstV1I GCAGC 9 cut(s) 61, 190, 256, 578, 823, 1087, 1196, 1426, 1454
BstX2I RGATCY 2 cut(s) 814, 1471
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 2 cut(s) 814, 1471
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 2 cut(s) 241, 1142
BtsCI GGATG 4 cut(s) 163, 429, 991, 1058
Cac8I GCNNGC 3 cut(s) 298, 596, 1497
CciI TCATGA 4 cut(s) 124, 565, 862, 982
CfoI GCGC 2 cut(s) 1058, 1447
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 3 cut(s) 239, 619, 1166
Csp6I GTAC 1 cut(s) 1245
CspAI ACCGGT 1 cut(s) 71
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviQI GTAC 1 cut(s) 1245
DdeI CTNAG 3 cut(s) 906, 996, 1251
DpnI GATC 6 cut(s) 37, 339, 816, 981, 1324, 1473
DpnII GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
DraI TTTAAA 1 cut(s) 843
DraIII CACNNNGTG 1 cut(s) 1043
Eam1104I CTCTTC 2 cut(s) 1321, 1446
EarI CTCTTC 2 cut(s) 1321, 1446
Ecl136II GAGCTC 2 cut(s) 130, 188
Eco130I CCWWGG 1 cut(s) 1143
Eco24I GRGCYC 2 cut(s) 132, 190
Eco31I GGTCTC 1 cut(s) 426
Eco32I GATATC 1 cut(s) 648
Eco47I GGWCC 2 cut(s) 619, 1166
Eco47III AGCGCT 1 cut(s) 1446
Eco53kI GAGCTC 2 cut(s) 130, 188
Eco81I CCTNAGG 1 cut(s) 996
EcoICRI GAGCTC 2 cut(s) 130, 188
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoO109I RGGNCCY 1 cut(s) 619
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 4 cut(s) 300, 606, 951, 1137
EcoRV GATATC 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 1143
EcoT22I ATGCAT 2 cut(s) 165, 1501
EcoT38I GRGCYC 2 cut(s) 132, 190
ErhI CCWWGG 1 cut(s) 1143
FaqI GGGAC 1 cut(s) 1270
FauI CCCGC 2 cut(s) 175, 265
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 1 cut(s) 882
FokI GGATG 4 cut(s) 150, 416, 978, 1065
FriOI GRGCYC 2 cut(s) 132, 190
FspBI CTAG 3 cut(s) 183, 1040, 1308
GlaI GCGC 2 cut(s) 1057, 1446
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 1448
HaeIII GGCC 2 cut(s) 241, 1142
HapII CCGG 2 cut(s) 72, 209
HhaI GCGC 2 cut(s) 1058, 1447
Hin6I GCGC 2 cut(s) 1056, 1445
HinP1I GCGC 2 cut(s) 1056, 1445
HindIII AAGCTT 2 cut(s) 92, 943
HinfI GANTC 3 cut(s) 350, 556, 1258
HpaII CCGG 2 cut(s) 72, 209
HphI GGTGA 3 cut(s) 287, 302, 700
Hpy166II GTNNAC 4 cut(s) 278, 586, 883, 1245
Hpy188I TCNGA 2 cut(s) 652, 1166
Hpy8I GTNNAC 4 cut(s) 278, 586, 883, 1245
HpyAV CCTTC 2 cut(s) 78, 838
HpyCH4III ACNGT 2 cut(s) 1151, 1492
HpyCH4IV ACGT 1 cut(s) 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 3 cut(s) 906, 996, 1251
HpySE526I ACGT 1 cut(s) 799
HspAI GCGC 2 cut(s) 1056, 1445
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
LmnI GCTCC 5 cut(s) 135, 211, 784, 1097, 1429
Lsp1109I GCAGC 9 cut(s) 61, 190, 256, 578, 823, 1087, 1196, 1426, 1454
LweI GCATC 6 cut(s) 172, 283, 694, 1043, 1441, 1486
MaeI CTAG 3 cut(s) 183, 1040, 1308
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 5 cut(s) 62, 211, 1082, 1198, 1285
MalI GATC 6 cut(s) 37, 339, 816, 981, 1324, 1473
MboI GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
MboII GAAGA 7 cut(s) 273, 432, 1032, 1153, 1308, 1463, 1481
MflI RGATCY 2 cut(s) 814, 1471
MhlI GDGCHC 3 cut(s) 132, 190, 781
MluCI AATT 6 cut(s) 29, 264, 680, 867, 968, 1364
MlyI GAGTC 2 cut(s) 359, 1252
MmeI TCCRAC 3 cut(s) 351, 521, 1318
Mph1103I ATGCAT 2 cut(s) 165, 1501
MroXI GAANNNNTTC 1 cut(s) 1315
MseI TTAA 4 cut(s) 314, 710, 842, 1400
MslI CAYNNNNRTG 3 cut(s) 506, 564, 609
MspA1I CMGCKG 2 cut(s) 591, 1250
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 2 cut(s) 72, 209
MspR9I CCNGG 4 cut(s) 302, 608, 953, 1139
MvaI CCWGG 4 cut(s) 302, 608, 953, 1139
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeII GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
NlaIV GGNNCC 6 cut(s) 310, 621, 1035, 1045, 1168, 1305
NmuCI GTSAC 2 cut(s) 1082, 1285
NsiI ATGCAT 2 cut(s) 165, 1501
NspI RCATGY 2 cut(s) 60, 1499
NspV TTCGAA 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 609
PaeI GCATGC 1 cut(s) 1499
PagI TCATGA 4 cut(s) 124, 565, 862, 982
PciI ACATGT 1 cut(s) 56
PdmI GAANNNNTTC 1 cut(s) 1315
PfeI GAWTC 1 cut(s) 556
PflMI CCANNNNNTGG 2 cut(s) 1138, 1300
PinAI ACCGGT 1 cut(s) 71
PleI GAGTC 2 cut(s) 358, 1252
PpsI GAGTC 2 cut(s) 358, 1252
PpuMI RGGWCCY 1 cut(s) 619
PscI ACATGT 1 cut(s) 56
Psp124BI GAGCTC 2 cut(s) 132, 190
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 4 cut(s) 300, 606, 951, 1137
PspGI CCWGG 4 cut(s) 300, 606, 951, 1137
PspN4I GGNNCC 6 cut(s) 310, 621, 1035, 1045, 1168, 1305
PspPI GGNCC 3 cut(s) 239, 619, 1166
PspPPI RGGWCCY 1 cut(s) 619
PstI CTGCAG 2 cut(s) 1105, 1444
PsuI RGATCY 2 cut(s) 814, 1471
PvuII CAGCTG 2 cut(s) 591, 1250
RsaI GTAC 1 cut(s) 1246
RsaNI GTAC 1 cut(s) 1245
RseI CAYNNNNRTG 3 cut(s) 506, 564, 609
SacI GAGCTC 2 cut(s) 132, 190
SaqAI TTAA 4 cut(s) 314, 710, 842, 1400
Sau3AI GATC 6 cut(s) 35, 337, 814, 979, 1322, 1471
Sau96I GGNCC 3 cut(s) 239, 619, 1166
SchI GAGTC 2 cut(s) 359, 1252
ScrFI CCNGG 4 cut(s) 302, 608, 953, 1139
SduI GDGCHC 3 cut(s) 132, 190, 781
SfaNI GCATC 6 cut(s) 172, 283, 694, 1043, 1441, 1486
SfcI CTRYAG 2 cut(s) 1101, 1440
SfuI TTCGAA 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 2 cut(s) 619, 1166
SmiMI CAYNNNNRTG 3 cut(s) 506, 564, 609
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
SphI GCATGC 1 cut(s) 1499
Sse9I AATT 6 cut(s) 29, 264, 680, 867, 968, 1364
SsiI CCGC 6 cut(s) 46, 168, 242, 272, 878, 1384
SspMI CTAG 3 cut(s) 183, 1040, 1308
SstI GAGCTC 2 cut(s) 132, 190
StyD4I CCNGG 4 cut(s) 300, 606, 951, 1137
StyI CCWWGG 1 cut(s) 1143
TaaI ACNGT 2 cut(s) 1151, 1492
TaiI ACGT 1 cut(s) 802
TaqI TCGA 5 cut(s) 34, 150, 389, 522, 675
TasI AATT 6 cut(s) 29, 264, 680, 867, 968, 1364
TatI WGTACW 1 cut(s) 1244
TauI GCSGC 2 cut(s) 49, 244
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 4 cut(s) 314, 710, 842, 1400
Tru9I TTAA 4 cut(s) 314, 710, 842, 1400
TseFI GTSAC 2 cut(s) 1082, 1285
TseI GCWGC 9 cut(s) 49, 203, 269, 591, 836, 1100, 1184, 1439, 1442
Tsp45I GTSAC 2 cut(s) 1082, 1285
Van91I CCANNNNNTGG 2 cut(s) 1138, 1300
Vha464I CTTAAG 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 619, 1166
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 1 cut(s) 680
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 2 cut(s) 60, 1499
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 1 cut(s) 1315
XspI CTAG 3 cut(s) 183, 1040, 1308
Zsp2I ATGCAT 2 cut(s) 165, 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.