Rh4AG129700

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001671
Physical Location & Seq
Reverse (-)
28095997 .. 28103606
7610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG129700.1

Sequence Viewer

Length: 1431 bp
ATGAGAGCAATTGCTCGTTCTATCCACCAATTTAGATCAGCTCTGCGGCGGCAAACATGTAATTACTCCACTGGTTTTGTGGGAAGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTCGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAAGCACCTTAACAGAACTTCAAAGGCTATATGATGAACTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAACTTCGAGGAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAGTTGGAGGAAAAAGAGGATTCATTTCCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATACTAAATCGTCTATTTAGACATCTATTTGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTGCTTTAAAGGAAAGATGTGTGGTTCATGAAATTGGTTCAGCAGTAGACTACCGAAAACTGACTTCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCAGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCTGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTCTGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCTTGGCCTTGGAGGGTGTCCCTATTTTTGGACTCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTACGAGAACAAGGGCAGACTGATGTGTACAGCTGAGGGGACTCCATTTGAACTTTTTGAAAGAATAGTGACAATCTCTGATGCCCAACAAGTGGCACCTAGAACCTCTTCGAGATCAAGGAAAAATGATGATTCGGAGCTATGTGTAGATAATGAATTGGGATTTGCTAAAGACCGCACCATTAGTAGGTATGTTCCTGTGCTGAATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

476

Amino Acids

53.61

Weight (kDa)

6.87

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 82 - 427 2.2e-89 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1312
AccB7I CCANNNNNTGG 2 cut(s) 1138, 1309
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 170
AciI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
AclWI GGATC 1 cut(s) 822
AcsI RAATTY 3 cut(s) 146, 172, 680
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 2 cut(s) 1220, 1246
AfiI CCNNNNNNNGG 5 cut(s) 461, 1138, 1163, 1309, 1404
AflII CTTAAG 1 cut(s) 950
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 8 cut(s) 209, 326, 444, 580, 629, 965, 1268, 1277
AjnI CCWGG 2 cut(s) 300, 606
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 2 cut(s) 190, 781
Alw26I GTCTC 1 cut(s) 426
AlwI GGATC 1 cut(s) 822
AoxI GGCC 2 cut(s) 239, 1140
ApeKI GCWGC 5 cut(s) 269, 591, 836, 1100, 1184
ApoI RAATTY 3 cut(s) 146, 172, 680
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AseI ATTAAT 1 cut(s) 710
Asp700I GAANNNNTTC 3 cut(s) 345, 960, 1324
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 302, 700
AxyI CCTNAGG 1 cut(s) 996
BanI GGYRCC 1 cut(s) 1312
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 432, 624
Bbv12I GWGCWC 2 cut(s) 190, 781
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 5 cut(s) 256, 578, 823, 1087, 1196
BccI CCATC 3 cut(s) 281, 405, 502
BciT130I CCWGG 2 cut(s) 302, 608
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 2 cut(s) 1040, 1317
BfmI CTRYAG 1 cut(s) 1101
BfrI CTTAAG 1 cut(s) 950
BisI GCNGC 7 cut(s) 47, 50, 270, 592, 837, 1101, 1185
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 7 cut(s) 48, 51, 271, 593, 838, 1102, 1186
Bme1390I CCNGG 2 cut(s) 302, 608
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 1035, 1314
BmrFI CCNGG 2 cut(s) 302, 608
BmsI GCATC 6 cut(s) 190, 214, 283, 349, 694, 1288
BpiI GAAGAC 2 cut(s) 432, 624
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 607, 1137, 1143
Bsc4I CCNNNNNNNGG 5 cut(s) 461, 1138, 1163, 1309, 1404
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 76, 496
Bse21I CCTNAGG 1 cut(s) 996
BseBI CCWGG 2 cut(s) 302, 608
BseDI CCNNGG 3 cut(s) 607, 1137, 1143
BseGI GGATG 3 cut(s) 429, 991, 1058
BseLI CCNNNNNNNGG 5 cut(s) 461, 1138, 1163, 1309, 1404
BseMII CTCAG 3 cut(s) 897, 987, 1242
BseNI ACTGG 2 cut(s) 76, 496
BseRI GAGGAG 2 cut(s) 495, 1013
BseXI GCAGC 5 cut(s) 256, 578, 823, 1087, 1196
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 241, 1142
BshNI GGYRCC 1 cut(s) 1312
BsiHKAI GWGCWC 2 cut(s) 190, 781
BsiSI CCGG 1 cut(s) 367
BslFI GGGAC 2 cut(s) 1139, 1270
BslI CCNNNNNNNGG 5 cut(s) 461, 1138, 1163, 1309, 1404
BsmAI GTCTC 1 cut(s) 426
BsmFI GGGAC 2 cut(s) 1139, 1270
BsnI GGCC 2 cut(s) 241, 1142
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 190, 781
Bsp1407I TGTACA 1 cut(s) 1244
Bsp143I GATC 4 cut(s) 35, 523, 814, 1331
Bsp1720I GCTNAGC 1 cut(s) 906
BspACI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
BspANI GGCC 2 cut(s) 241, 1142
BspCNI CTCAG 3 cut(s) 898, 988, 1243
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 2 cut(s) 124, 862
BspLI GGNNCC 2 cut(s) 1035, 1314
BspMAI CTGCAG 1 cut(s) 1105
BspPI GGATC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 1312
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 1244
BsrI ACTGG 2 cut(s) 76, 496
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 3 cut(s) 607, 1137, 1143
BssMI GATC 4 cut(s) 35, 523, 814, 1331
BssT1I CCWWGG 2 cut(s) 1137, 1143
Bst2UI CCWGG 2 cut(s) 302, 608
Bst4CI ACNGT 1 cut(s) 438
Bst6I CTCTTC 1 cut(s) 1330
BstAFI CTTAAG 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 1244
BstC8I GCNNGC 6 cut(s) 229, 274, 298, 364, 368, 596
BstDEI CTNAG 3 cut(s) 906, 996, 1251
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 429, 991, 1058
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 4 cut(s) 38, 526, 817, 1334
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 4 cut(s) 35, 523, 814, 1331
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 2 cut(s) 302, 608
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 300, 606
BstSFI CTRYAG 1 cut(s) 1101
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 5 cut(s) 256, 578, 823, 1087, 1196
BstV2I GAAGAC 2 cut(s) 432, 624
BstX2I RGATCY 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 2 cut(s) 241, 1142
BtsCI GGATG 3 cut(s) 429, 991, 1058
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 6 cut(s) 229, 274, 298, 364, 368, 596
CciI TCATGA 2 cut(s) 124, 862
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 1219, 1245
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 6 cut(s) 57, 125, 502, 566, 685, 863
CviQI GTAC 2 cut(s) 1219, 1245
DdeI CTNAG 3 cut(s) 906, 996, 1251
DpnI GATC 4 cut(s) 37, 525, 816, 1333
DpnII GATC 4 cut(s) 35, 523, 814, 1331
DraI TTTAAA 1 cut(s) 843
DraIII CACNNNGTG 1 cut(s) 1043
Eam1104I CTCTTC 1 cut(s) 1330
EarI CTCTTC 1 cut(s) 1330
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 188
Eco130I CCWWGG 2 cut(s) 1137, 1143
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 426
Eco32I GATATC 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 188
Eco81I CCTNAGG 1 cut(s) 996
EcoICRI GAGCTC 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 300, 606
EcoRV GATATC 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 1137, 1143
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 1137, 1143
FaeI CATG 6 cut(s) 60, 128, 505, 569, 688, 866
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FaqI GGGAC 2 cut(s) 1139, 1270
FatI CATG 6 cut(s) 56, 124, 501, 565, 684, 862
FauI CCCGC 2 cut(s) 175, 265
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 7 cut(s) 47, 50, 270, 592, 837, 1101, 1185
FokI GGATG 3 cut(s) 416, 978, 1065
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 7 cut(s) 47, 50, 270, 592, 837, 1101, 1185
FspBI CTAG 2 cut(s) 1040, 1317
GluI GCNGC 7 cut(s) 47, 50, 270, 592, 837, 1101, 1185
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 2 cut(s) 241, 1142
HapII CCGG 1 cut(s) 367
Hin1II CATG 6 cut(s) 60, 128, 505, 569, 688, 866
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 5 cut(s) 556, 1167, 1258, 1349, 1423
HpaII CCGG 1 cut(s) 367
HphI GGTGA 2 cut(s) 302, 700
Hpy166II GTNNAC 3 cut(s) 586, 883, 1245
Hpy188I TCNGA 3 cut(s) 652, 1297, 1354
Hpy8I GTNNAC 3 cut(s) 586, 883, 1245
HpyAV CCTTC 2 cut(s) 78, 396
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 1 cut(s) 799
HpyCH4V TGCA 8 cut(s) 227, 537, 638, 707, 808, 1058, 1103, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 3 cut(s) 906, 996, 1251
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 6 cut(s) 60, 128, 505, 569, 688, 866
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
Kzo9I GATC 4 cut(s) 35, 523, 814, 1331
LmnI GCTCC 3 cut(s) 784, 1097, 1354
Lsp1109I GCAGC 5 cut(s) 256, 578, 823, 1087, 1196
LweI GCATC 6 cut(s) 190, 214, 283, 349, 694, 1288
MaeI CTAG 2 cut(s) 1040, 1317
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 5 cut(s) 211, 688, 1082, 1198, 1285
MalI GATC 4 cut(s) 37, 525, 816, 1333
MboI GATC 4 cut(s) 35, 523, 814, 1331
MboII GAAGA 5 cut(s) 273, 432, 629, 1032, 1317
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 190, 781
MlyI GAGTC 2 cut(s) 1161, 1252
MmeI TCCRAC 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 366
MroXI GAANNNNTTC 3 cut(s) 345, 960, 1324
MseI TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
MslI CAYNNNNRTG 3 cut(s) 506, 564, 609
MspA1I CMGCKG 3 cut(s) 356, 591, 1250
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 2 cut(s) 302, 608
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 2 cut(s) 302, 608
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 306
NaeI GCCGGC 1 cut(s) 368
NdeII GATC 4 cut(s) 35, 523, 814, 1331
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 6 cut(s) 60, 128, 505, 569, 688, 866
NlaIV GGNNCC 2 cut(s) 1035, 1314
NmuCI GTSAC 3 cut(s) 688, 1082, 1285
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 609
PagI TCATGA 2 cut(s) 124, 862
PciI ACATGT 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 368
PdmI GAANNNNTTC 3 cut(s) 345, 960, 1324
PfeI GAWTC 3 cut(s) 556, 1349, 1423
PflMI CCANNNNNTGG 2 cut(s) 1138, 1309
PkrI GCNGC 7 cut(s) 48, 51, 271, 593, 838, 1102, 1186
PleI GAGTC 2 cut(s) 1161, 1252
PpsI GAGTC 2 cut(s) 1161, 1252
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 300, 606
PspGI CCWGG 2 cut(s) 300, 606
PspN4I GGNNCC 2 cut(s) 1035, 1314
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 1105
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 3 cut(s) 356, 591, 1250
RsaI GTAC 2 cut(s) 1220, 1246
RsaNI GTAC 2 cut(s) 1219, 1245
RseI CAYNNNNRTG 3 cut(s) 506, 564, 609
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
SatI GCNGC 7 cut(s) 47, 50, 270, 592, 837, 1101, 1185
Sau3AI GATC 4 cut(s) 35, 523, 814, 1331
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 2 cut(s) 1161, 1252
ScrFI CCNGG 2 cut(s) 302, 608
SduI GDGCHC 2 cut(s) 190, 781
SfaNI GCATC 6 cut(s) 190, 214, 283, 349, 694, 1288
SfcI CTRYAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 3 cut(s) 506, 564, 609
SmlI CTYRAG 2 cut(s) 181, 950
SmoI CTYRAG 2 cut(s) 181, 950
SsiI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
SspMI CTAG 2 cut(s) 1040, 1317
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 2 cut(s) 300, 606
StyI CCWWGG 2 cut(s) 1137, 1143
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 1 cut(s) 802
TaqI TCGA 5 cut(s) 150, 198, 389, 522, 1328
TatI WGTACW 1 cut(s) 1244
TauI GCSGC 2 cut(s) 49, 52
TfiI GAWTC 3 cut(s) 556, 1349, 1423
Tru1I TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
Tru9I TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 3 cut(s) 688, 1082, 1285
TseI GCWGC 5 cut(s) 269, 591, 836, 1100, 1184
Tsp45I GTSAC 3 cut(s) 688, 1082, 1285
TspRI CASTG 1 cut(s) 76
Van91I CCANNNNNTGG 2 cut(s) 1138, 1309
Vha464I CTTAAG 1 cut(s) 950
VspI ATTAAT 1 cut(s) 710
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 146, 172, 680
XceI RCATGY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 3 cut(s) 345, 960, 1324
XspI CTAG 2 cut(s) 1040, 1317
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.