Rh4CG137000

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001671
Physical Location & Seq
Reverse (-)
27090205 .. 27096752
6548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG137000.1

Sequence Viewer

Length: 1131 bp
ATGAGAGCAATTGCTCGTTCCATCCACCAATTTAGATCAGCTCTGCGGCGGCAAACATGTAATTACTCCACTGGTTTTGTGGGAAGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCGCATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACATCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAAGCACCTTAACAGAACTTCAAAGGCTATATGATGAACTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAACTTCGAGAAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAAATACAATGCAGTTGGAGGAAAAAGAGGATTCATTTCCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATACTAAATCGTCTATTTAGACATCTATTTGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTGCTTTAAAGGAAAGATGTGTGGTTCATGAAATTGGTTCAGCAGTAGACTACCGAAAACTGACTTCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCAGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCTGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTCTGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

376

Amino Acids

42.26

Weight (kDa)

6.8

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 82 - 374 3.7e-73 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 170
AciI CCGC 5 cut(s) 46, 49, 88, 168, 272
AclWI GGATC 1 cut(s) 822
AcsI RAATTY 3 cut(s) 146, 172, 680
AdeI CACNNNGTG 1 cut(s) 1043
AfiI CCNNNNNNNGG 1 cut(s) 461
AflII CTTAAG 1 cut(s) 950
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 6 cut(s) 209, 326, 444, 580, 629, 965
AjnI CCWGG 2 cut(s) 300, 606
AleI CACNNNNGTG 1 cut(s) 609
AluBI AGCT 8 cut(s) 41, 94, 108, 188, 356, 591, 989, 1100
AluI AGCT 8 cut(s) 41, 94, 108, 188, 356, 591, 989, 1100
Alw21I GWGCWC 2 cut(s) 190, 781
Alw26I GTCTC 1 cut(s) 426
AlwI GGATC 1 cut(s) 822
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 4 cut(s) 269, 591, 836, 1100
ApoI RAATTY 3 cut(s) 146, 172, 680
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AseI ATTAAT 1 cut(s) 710
Asp700I GAANNNNTTC 2 cut(s) 345, 960
AspLEI GCGC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 302, 700
AxyI CCTNAGG 1 cut(s) 996
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 432, 624
Bbv12I GWGCWC 2 cut(s) 190, 781
BbvI GCAGC 4 cut(s) 256, 578, 823, 1087
BccI CCATC 4 cut(s) 29, 281, 405, 502
BciT130I CCWGG 2 cut(s) 302, 608
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 1 cut(s) 1040
BfmI CTRYAG 1 cut(s) 1101
BfrI CTTAAG 1 cut(s) 950
BisI GCNGC 6 cut(s) 47, 50, 270, 592, 837, 1101
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 6 cut(s) 48, 51, 271, 593, 838, 1102
Bme1390I CCNGG 2 cut(s) 302, 608
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 1035
BmrFI CCNGG 2 cut(s) 302, 608
BmsI GCATC 5 cut(s) 190, 214, 283, 349, 694
BpiI GAAGAC 2 cut(s) 432, 624
BpmI CTGGAG 1 cut(s) 765
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 607
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 76, 496
Bse21I CCTNAGG 1 cut(s) 996
BseBI CCWGG 2 cut(s) 302, 608
BseDI CCNNGG 1 cut(s) 607
BseGI GGATG 5 cut(s) 21, 163, 429, 991, 1058
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMII CTCAG 2 cut(s) 897, 987
BseNI ACTGG 2 cut(s) 76, 496
BseRI GAGGAG 2 cut(s) 495, 1013
BseXI GCAGC 4 cut(s) 256, 578, 823, 1087
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 241
BsiHKAI GWGCWC 2 cut(s) 190, 781
BsiSI CCGG 1 cut(s) 367
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 426
BsnI GGCC 1 cut(s) 241
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 2 cut(s) 190, 781
Bsp143I GATC 3 cut(s) 35, 523, 814
Bsp1720I GCTNAGC 1 cut(s) 906
BspACI CCGC 5 cut(s) 46, 49, 88, 168, 272
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 2 cut(s) 898, 988
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 1 cut(s) 1035
BspMAI CTGCAG 1 cut(s) 1105
BspPI GGATC 1 cut(s) 822
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 2 cut(s) 76, 496
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 607
BssMI GATC 3 cut(s) 35, 523, 814
Bst2UI CCWGG 2 cut(s) 302, 608
Bst4CI ACNGT 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 950
BstC8I GCNNGC 6 cut(s) 229, 274, 298, 364, 368, 596
BstDEI CTNAG 2 cut(s) 906, 996
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 5 cut(s) 21, 163, 429, 991, 1058
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 124
BstKTI GATC 3 cut(s) 38, 526, 817
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 3 cut(s) 35, 523, 814
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 2 cut(s) 302, 608
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 300, 606
BstSFI CTRYAG 1 cut(s) 1101
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 4 cut(s) 256, 578, 823, 1087
BstV2I GAAGAC 2 cut(s) 432, 624
BstX2I RGATCY 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 1 cut(s) 814
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 1 cut(s) 241
BtsCI GGATG 5 cut(s) 21, 163, 429, 991, 1058
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 6 cut(s) 229, 274, 298, 364, 368, 596
CciI TCATGA 1 cut(s) 862
CfoI GCGC 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 6 cut(s) 57, 125, 502, 566, 685, 863
DdeI CTNAG 2 cut(s) 906, 996
DpnI GATC 3 cut(s) 37, 525, 816
DpnII GATC 3 cut(s) 35, 523, 814
DraI TTTAAA 1 cut(s) 843
DraIII CACNNNGTG 1 cut(s) 1043
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 426
Eco32I GATATC 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 188
Eco81I CCTNAGG 1 cut(s) 996
EcoICRI GAGCTC 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 300, 606
EcoRV GATATC 1 cut(s) 648
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 6 cut(s) 60, 128, 505, 569, 688, 866
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 6 cut(s) 56, 124, 501, 565, 684, 862
FauI CCCGC 2 cut(s) 175, 265
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 47, 50, 270, 592, 837, 1101
FokI GGATG 5 cut(s) 8, 150, 416, 978, 1065
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 6 cut(s) 47, 50, 270, 592, 837, 1101
FspBI CTAG 1 cut(s) 1040
GlaI GCGC 1 cut(s) 123
GluI GCNGC 6 cut(s) 47, 50, 270, 592, 837, 1101
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 1 cut(s) 241
HapII CCGG 1 cut(s) 367
HhaI GCGC 1 cut(s) 124
Hin1II CATG 6 cut(s) 60, 128, 505, 569, 688, 866
Hin6I GCGC 1 cut(s) 122
HinP1I GCGC 1 cut(s) 122
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 1 cut(s) 556
HpaII CCGG 1 cut(s) 367
HphI GGTGA 2 cut(s) 302, 700
Hpy166II GTNNAC 2 cut(s) 586, 883
Hpy188I TCNGA 1 cut(s) 652
Hpy188III TCNNGA 9 cut(s) 183, 390, 399, 626, 674, 782, 863, 965, 995
Hpy8I GTNNAC 2 cut(s) 586, 883
HpyAV CCTTC 2 cut(s) 78, 396
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 1 cut(s) 799
HpyCH4V TGCA 8 cut(s) 227, 537, 638, 707, 808, 1058, 1103, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 2 cut(s) 906, 996
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 6 cut(s) 60, 128, 505, 569, 688, 866
HspAI GCGC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
Kzo9I GATC 3 cut(s) 35, 523, 814
LmnI GCTCC 2 cut(s) 784, 1097
Lsp1109I GCAGC 4 cut(s) 256, 578, 823, 1087
LweI GCATC 5 cut(s) 190, 214, 283, 349, 694
MaeI CTAG 1 cut(s) 1040
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 3 cut(s) 211, 688, 1082
MalI GATC 3 cut(s) 37, 525, 816
MboI GATC 3 cut(s) 35, 523, 814
MboII GAAGA 4 cut(s) 273, 432, 629, 1032
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 190, 781
MluCI AATT 9 cut(s) 9, 29, 61, 146, 172, 264, 680, 867, 968
MmeI TCCRAC 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 366
MroXI GAANNNNTTC 2 cut(s) 345, 960
MseI TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
MslI CAYNNNNRTG 3 cut(s) 506, 564, 609
MspA1I CMGCKG 2 cut(s) 356, 591
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 2 cut(s) 302, 608
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 2 cut(s) 302, 608
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 306
NaeI GCCGGC 1 cut(s) 368
NdeII GATC 3 cut(s) 35, 523, 814
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 6 cut(s) 60, 128, 505, 569, 688, 866
NlaIV GGNNCC 1 cut(s) 1035
NmuCI GTSAC 2 cut(s) 688, 1082
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 609
PagI TCATGA 1 cut(s) 862
PciI ACATGT 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 368
PdmI GAANNNNTTC 2 cut(s) 345, 960
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 6 cut(s) 48, 51, 271, 593, 838, 1102
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 710
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 2 cut(s) 300, 606
PspGI CCWGG 2 cut(s) 300, 606
PspN4I GGNNCC 1 cut(s) 1035
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 1105
PsuI RGATCY 1 cut(s) 814
PvuII CAGCTG 2 cut(s) 356, 591
RseI CAYNNNNRTG 3 cut(s) 506, 564, 609
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
SatI GCNGC 6 cut(s) 47, 50, 270, 592, 837, 1101
Sau3AI GATC 3 cut(s) 35, 523, 814
Sau96I GGNCC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 302, 608
SduI GDGCHC 2 cut(s) 190, 781
SfaNI GCATC 5 cut(s) 190, 214, 283, 349, 694
SfcI CTRYAG 1 cut(s) 1101
SmiMI CAYNNNNRTG 3 cut(s) 506, 564, 609
SmlI CTYRAG 2 cut(s) 181, 950
SmoI CTYRAG 2 cut(s) 181, 950
Sse9I AATT 9 cut(s) 9, 29, 61, 146, 172, 264, 680, 867, 968
SsiI CCGC 5 cut(s) 46, 49, 88, 168, 272
SspMI CTAG 1 cut(s) 1040
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 2 cut(s) 300, 606
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 1 cut(s) 802
TaqI TCGA 3 cut(s) 150, 389, 522
TasI AATT 9 cut(s) 9, 29, 61, 146, 172, 264, 680, 867, 968
TauI GCSGC 2 cut(s) 49, 52
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
Tru9I TTAA 6 cut(s) 314, 459, 710, 842, 951, 1028
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 2 cut(s) 688, 1082
TseI GCWGC 4 cut(s) 269, 591, 836, 1100
Tsp45I GTSAC 2 cut(s) 688, 1082
TspDTI ATGAA 9 cut(s) 141, 354, 418, 548, 673, 693, 810, 851, 879
TspRI CASTG 1 cut(s) 76
Vha464I CTTAAG 1 cut(s) 950
VspI ATTAAT 1 cut(s) 710
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 146, 172, 680
XceI RCATGY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 2 cut(s) 345, 960
XspI CTAG 1 cut(s) 1040
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.