Rw4G010480

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001671
Physical Location & Seq
Reverse (-)
24312803 .. 24318804
6002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010480.1

Sequence Viewer

Length: 1512 bp
ATGAGAGCAATTGCTCGTTCTATCCACCAATTTAGATCAGCTCTGCGGCGGCAGACATGTAATTACTCCAGTGGTTTTGTGAGAAGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGGGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAAGCACCTTAACAGAACTTCAAAGGCTATATGATGAACTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAACTTCGAGGAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAGTTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATACTAAATCGTCTATTTAGACATCTATTTGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTGCTTTGAAGGAAAGATGTGTGGTTCATGAAATTGGTTCAGCAGTTGACTACCGAAAACTGACTTCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCAGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCTGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTCTGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCTTGGCCTTGGAGGGTGTCCCTATTTTTGGACTCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTACGAGAACAAGGGCAGACTGATGTGTACAGCTGAGGGGACTCCATTTGAACTTTTTGAAAGAATAGTGACAATCTCTGATGCCCAACAAGTGGCACCTAGAACCTCTTCGAGATCAAGGAAAAATGATGATTCGGAGCTTTGTGTAGATAATGAATTGGGATTTGCTAAAGACCGCACCATTAGTAGATTAACAGAAATGAATAGCAAAGAGTACTTGGAGCATCATGCTGCAGCGCTGGAAGAGAACGACTCCCATGAAGATCTGAATCAGGATAAAACCGTGCACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

503

Amino Acids

56.82

Weight (kDa)

6.17

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 82 - 427 2.7e-89 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1312
AccB7I CCANNNNNTGG 2 cut(s) 1138, 1309
AccII CGCG 1 cut(s) 170
AciI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
AclWI GGATC 1 cut(s) 822
AcsI RAATTY 3 cut(s) 146, 172, 680
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 3 cut(s) 1220, 1246, 1433
AfeI AGCGCT 1 cut(s) 1455
AfiI CCNNNNNNNGG 4 cut(s) 461, 1138, 1163, 1309
AflII CTTAAG 1 cut(s) 950
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 9 cut(s) 209, 326, 444, 580, 629, 844, 965, 1268, 1277
AjnI CCWGG 2 cut(s) 300, 606
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 2 cut(s) 781, 1506
Alw26I GTCTC 1 cut(s) 426
Alw44I GTGCAC 1 cut(s) 1502
AlwI GGATC 1 cut(s) 822
Aor51HI AGCGCT 1 cut(s) 1455
AoxI GGCC 2 cut(s) 239, 1140
ApaLI GTGCAC 1 cut(s) 1502
ApeKI GCWGC 7 cut(s) 269, 591, 836, 1100, 1184, 1448, 1451
ApoI RAATTY 3 cut(s) 146, 172, 680
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AseI ATTAAT 1 cut(s) 710
Asp700I GAANNNNTTC 3 cut(s) 345, 960, 1324
AspLEI GCGC 1 cut(s) 1456
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 302, 700
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 1 cut(s) 1506
BanI GGYRCC 1 cut(s) 1312
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 432, 624
Bbv12I GWGCWC 2 cut(s) 781, 1506
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 7 cut(s) 256, 578, 823, 1087, 1196, 1435, 1463
BccI CCATC 3 cut(s) 281, 405, 502
BciT130I CCWGG 2 cut(s) 302, 608
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 2 cut(s) 1040, 1317
BfmI CTRYAG 2 cut(s) 1101, 1449
BfoI RGCGCY 1 cut(s) 1457
BfrI CTTAAG 1 cut(s) 950
BglII AGATCT 1 cut(s) 1480
BisI GCNGC 9 cut(s) 47, 50, 270, 592, 837, 1101, 1185, 1449, 1452
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 9 cut(s) 48, 51, 271, 593, 838, 1102, 1186, 1450, 1453
BmcAI AGTACT 1 cut(s) 1433
Bme1390I CCNGG 2 cut(s) 302, 608
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 1035, 1314
BmrFI CCNGG 2 cut(s) 302, 608
BmsI GCATC 7 cut(s) 190, 214, 283, 349, 694, 1288, 1450
BpiI GAAGAC 2 cut(s) 432, 624
BplI GAGNNNNNCTC 2 cut(s) 1454, 1486
BpmI CTGGAG 2 cut(s) 52, 765
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu1102I GCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 1485
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 607, 1137, 1143
Bsc4I CCNNNNNNNGG 4 cut(s) 461, 1138, 1163, 1309
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 69, 496
Bse21I CCTNAGG 1 cut(s) 996
Bse8I GATNNNNATC 1 cut(s) 1485
BseBI CCWGG 2 cut(s) 302, 608
BseDI CCNNGG 3 cut(s) 607, 1137, 1143
BseGI GGATG 3 cut(s) 429, 991, 1058
BseJI GATNNNNATC 1 cut(s) 1485
BseLI CCNNNNNNNGG 4 cut(s) 461, 1138, 1163, 1309
BseMII CTCAG 3 cut(s) 897, 987, 1242
BseNI ACTGG 2 cut(s) 69, 496
BseRI GAGGAG 2 cut(s) 495, 1013
BseSI GKGCMC 1 cut(s) 1506
BseXI GCAGC 7 cut(s) 256, 578, 823, 1087, 1196, 1435, 1463
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 241, 1142
BshNI GGYRCC 1 cut(s) 1312
BsiHKAI GWGCWC 2 cut(s) 781, 1506
BsiSI CCGG 1 cut(s) 367
BslFI GGGAC 2 cut(s) 1139, 1270
BslI CCNNNNNNNGG 4 cut(s) 461, 1138, 1163, 1309
BsmAI GTCTC 1 cut(s) 426
BsmFI GGGAC 2 cut(s) 1139, 1270
BsnI GGCC 2 cut(s) 241, 1142
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 3 cut(s) 190, 781, 1506
Bsp1407I TGTACA 1 cut(s) 1244
Bsp143I GATC 5 cut(s) 35, 523, 814, 1331, 1480
Bsp1720I GCTNAGC 1 cut(s) 906
BspACI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
BspANI GGCC 2 cut(s) 241, 1142
BspCNI CTCAG 3 cut(s) 898, 988, 1243
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 3 cut(s) 124, 565, 862
BspLI GGNNCC 2 cut(s) 1035, 1314
BspMAI CTGCAG 2 cut(s) 1105, 1453
BspPI GGATC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 1312
BspTI CTTAAG 1 cut(s) 950
BspTNI GGTCTC 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 1244
BsrI ACTGG 2 cut(s) 69, 496
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 3 cut(s) 607, 1137, 1143
BssMI GATC 5 cut(s) 35, 523, 814, 1331, 1480
BssT1I CCWWGG 2 cut(s) 1137, 1143
Bst2UI CCWGG 2 cut(s) 302, 608
Bst4CI ACNGT 2 cut(s) 438, 1501
Bst6I CTCTTC 2 cut(s) 1330, 1455
BstAFI CTTAAG 1 cut(s) 950
BstAUI TGTACA 1 cut(s) 1244
BstC8I GCNNGC 7 cut(s) 229, 274, 298, 364, 368, 596, 1506
BstDEI CTNAG 3 cut(s) 906, 996, 1251
BstENI CCTNNNNNAGG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 429, 991, 1058
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 1457
BstHHI GCGC 1 cut(s) 1456
BstKTI GATC 5 cut(s) 38, 526, 817, 1334, 1483
BstMAI GTCTC 1 cut(s) 426
BstMBI GATC 5 cut(s) 35, 523, 814, 1331, 1480
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 2 cut(s) 302, 608
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 2 cut(s) 300, 606
BstSFI CTRYAG 2 cut(s) 1101, 1449
BstSLI GKGCMC 1 cut(s) 1506
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 7 cut(s) 256, 578, 823, 1087, 1196, 1435, 1463
BstV2I GAAGAC 2 cut(s) 432, 624
BstX2I RGATCY 2 cut(s) 814, 1480
BstXI CCANNNNNNTGG 1 cut(s) 508
BstYI RGATCY 2 cut(s) 814, 1480
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 2 cut(s) 241, 1142
BtsCI GGATG 3 cut(s) 429, 991, 1058
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 7 cut(s) 229, 274, 298, 364, 368, 596, 1506
CciI TCATGA 3 cut(s) 124, 565, 862
CfoI GCGC 1 cut(s) 1456
Cfr10I RCCGGY 1 cut(s) 366
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 3 cut(s) 1219, 1245, 1432
CviAII CATG 9 cut(s) 57, 125, 502, 566, 685, 863, 1445, 1475, 1509
CviQI GTAC 3 cut(s) 1219, 1245, 1432
DdeI CTNAG 3 cut(s) 906, 996, 1251
DpnI GATC 5 cut(s) 37, 525, 816, 1333, 1482
DpnII GATC 5 cut(s) 35, 523, 814, 1331, 1480
DraIII CACNNNGTG 1 cut(s) 1043
Eam1104I CTCTTC 2 cut(s) 1330, 1455
EarI CTCTTC 2 cut(s) 1330, 1455
EciI GGCGGA 1 cut(s) 103
Eco130I CCWWGG 2 cut(s) 1137, 1143
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 426
Eco32I GATATC 1 cut(s) 648
Eco47III AGCGCT 1 cut(s) 1455
Eco81I CCTNAGG 1 cut(s) 996
EcoNI CCTNNNNNAGG 1 cut(s) 459
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 2 cut(s) 300, 606
EcoRV GATATC 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 1137, 1143
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 1137, 1143
FaeI CATG 9 cut(s) 60, 128, 505, 569, 688, 866, 1448, 1478, 1512
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FaqI GGGAC 2 cut(s) 1139, 1270
FatI CATG 9 cut(s) 56, 124, 501, 565, 684, 862, 1444, 1474, 1508
FauI CCCGC 2 cut(s) 175, 265
Fnu4HI GCNGC 9 cut(s) 47, 50, 270, 592, 837, 1101, 1185, 1449, 1452
FokI GGATG 3 cut(s) 416, 978, 1065
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 9 cut(s) 47, 50, 270, 592, 837, 1101, 1185, 1449, 1452
FspBI CTAG 2 cut(s) 1040, 1317
GlaI GCGC 1 cut(s) 1455
GluI GCNGC 9 cut(s) 47, 50, 270, 592, 837, 1101, 1185, 1449, 1452
GsuI CTGGAG 2 cut(s) 52, 765
HaeII RGCGCY 1 cut(s) 1457
HaeIII GGCC 2 cut(s) 241, 1142
HapII CCGG 1 cut(s) 367
HhaI GCGC 1 cut(s) 1456
Hin1II CATG 9 cut(s) 60, 128, 505, 569, 688, 866, 1448, 1478, 1512
Hin6I GCGC 1 cut(s) 1454
HinP1I GCGC 1 cut(s) 1454
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 6 cut(s) 556, 1167, 1258, 1349, 1469, 1486
HpaII CCGG 1 cut(s) 367
HphI GGTGA 2 cut(s) 302, 700
Hpy166II GTNNAC 4 cut(s) 586, 883, 1245, 1504
Hpy188I TCNGA 4 cut(s) 652, 1297, 1354, 1485
Hpy8I GTNNAC 4 cut(s) 586, 883, 1245, 1504
HpyAV CCTTC 3 cut(s) 78, 396, 838
HpyCH4III ACNGT 2 cut(s) 438, 1501
HpyCH4IV ACGT 1 cut(s) 799
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 3 cut(s) 906, 996, 1251
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 9 cut(s) 60, 128, 505, 569, 688, 866, 1448, 1478, 1512
HspAI GCGC 1 cut(s) 1454
KroI GCCGGC 1 cut(s) 366
KroNI GCCGGC 1 cut(s) 368
Kzo9I GATC 5 cut(s) 35, 523, 814, 1331, 1480
LmnI GCTCC 4 cut(s) 784, 1097, 1354, 1438
Lsp1109I GCAGC 7 cut(s) 256, 578, 823, 1087, 1196, 1435, 1463
LweI GCATC 7 cut(s) 190, 214, 283, 349, 694, 1288, 1450
MaeI CTAG 2 cut(s) 1040, 1317
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 5 cut(s) 211, 688, 1082, 1198, 1285
MalI GATC 5 cut(s) 37, 525, 816, 1333, 1482
MboI GATC 5 cut(s) 35, 523, 814, 1331, 1480
MboII GAAGA 7 cut(s) 273, 432, 629, 1032, 1317, 1472, 1490
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 2 cut(s) 814, 1480
MhlI GDGCHC 3 cut(s) 190, 781, 1506
MlyI GAGTC 3 cut(s) 1161, 1252, 1463
MmeI TCCRAC 1 cut(s) 521
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 366
MroXI GAANNNNTTC 3 cut(s) 345, 960, 1324
MseI TTAA 6 cut(s) 314, 459, 710, 951, 1028, 1409
MslI CAYNNNNRTG 3 cut(s) 506, 564, 609
MspA1I CMGCKG 3 cut(s) 356, 591, 1250
MspCI CTTAAG 1 cut(s) 950
MspI CCGG 1 cut(s) 367
MspR9I CCNGG 2 cut(s) 302, 608
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 2 cut(s) 302, 608
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 306
NaeI GCCGGC 1 cut(s) 368
NdeII GATC 5 cut(s) 35, 523, 814, 1331, 1480
NgoMIV GCCGGC 1 cut(s) 366
NlaIII CATG 9 cut(s) 60, 128, 505, 569, 688, 866, 1448, 1478, 1512
NlaIV GGNNCC 2 cut(s) 1035, 1314
NmuCI GTSAC 3 cut(s) 688, 1082, 1285
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 609
PagI TCATGA 3 cut(s) 124, 565, 862
PciI ACATGT 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 368
PdmI GAANNNNTTC 3 cut(s) 345, 960, 1324
PfeI GAWTC 3 cut(s) 556, 1349, 1486
PflMI CCANNNNNTGG 2 cut(s) 1138, 1309
PkrI GCNGC 9 cut(s) 48, 51, 271, 593, 838, 1102, 1186, 1450, 1453
PleI GAGTC 3 cut(s) 1161, 1252, 1463
PpsI GAGTC 3 cut(s) 1161, 1252, 1463
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 710
Psp6I CCWGG 2 cut(s) 300, 606
PspGI CCWGG 2 cut(s) 300, 606
PspN4I GGNNCC 2 cut(s) 1035, 1314
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 2 cut(s) 1105, 1453
PsuI RGATCY 2 cut(s) 814, 1480
PvuII CAGCTG 3 cut(s) 356, 591, 1250
RsaI GTAC 3 cut(s) 1220, 1246, 1433
RsaNI GTAC 3 cut(s) 1219, 1245, 1432
RseI CAYNNNNRTG 3 cut(s) 506, 564, 609
SaqAI TTAA 6 cut(s) 314, 459, 710, 951, 1028, 1409
SatI GCNGC 9 cut(s) 47, 50, 270, 592, 837, 1101, 1185, 1449, 1452
Sau3AI GATC 5 cut(s) 35, 523, 814, 1331, 1480
Sau96I GGNCC 1 cut(s) 239
ScaI AGTACT 1 cut(s) 1433
SchI GAGTC 3 cut(s) 1161, 1252, 1463
ScrFI CCNGG 2 cut(s) 302, 608
SduI GDGCHC 3 cut(s) 190, 781, 1506
SfaNI GCATC 7 cut(s) 190, 214, 283, 349, 694, 1288, 1450
SfcI CTRYAG 2 cut(s) 1101, 1449
SmiMI CAYNNNNRTG 3 cut(s) 506, 564, 609
SmlI CTYRAG 2 cut(s) 181, 950
SmoI CTYRAG 2 cut(s) 181, 950
SsiI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1393
SspMI CTAG 2 cut(s) 1040, 1317
StyD4I CCNGG 2 cut(s) 300, 606
StyI CCWWGG 2 cut(s) 1137, 1143
TaaI ACNGT 2 cut(s) 438, 1501
TaiI ACGT 1 cut(s) 802
TaqI TCGA 4 cut(s) 150, 389, 522, 1328
TatI WGTACW 2 cut(s) 1244, 1431
TauI GCSGC 2 cut(s) 49, 52
TfiI GAWTC 3 cut(s) 556, 1349, 1486
Tru1I TTAA 6 cut(s) 314, 459, 710, 951, 1028, 1409
Tru9I TTAA 6 cut(s) 314, 459, 710, 951, 1028, 1409
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 3 cut(s) 688, 1082, 1285
TseI GCWGC 7 cut(s) 269, 591, 836, 1100, 1184, 1448, 1451
Tsp45I GTSAC 3 cut(s) 688, 1082, 1285
TspRI CASTG 1 cut(s) 76
Van91I CCANNNNNTGG 2 cut(s) 1138, 1309
Vha464I CTTAAG 1 cut(s) 950
VneI GTGCAC 1 cut(s) 1502
VspI ATTAAT 1 cut(s) 710
XagI CCTNNNNNAGG 1 cut(s) 459
XapI RAATTY 3 cut(s) 146, 172, 680
XceI RCATGY 1 cut(s) 60
XmnI GAANNNNTTC 3 cut(s) 345, 960, 1324
XspI CTAG 2 cut(s) 1040, 1317
ZrmI AGTACT 1 cut(s) 1433
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.