Rroxscaffold_5G00349450

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001671
Physical Location & Seq
Reverse (-)
22459746 .. 22465900
6155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349450.1

Sequence Viewer

Length: 939 bp
ATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAAGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATACTAAATCGTCTATTTAGACAGCTATTCGACAATGGCATTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTGCTTTAAAGGAAAGATGTGTGGTTCATGAAATTGGTTCAGCAGTAGACTACCGAAAACTGACTTCAGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCAGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCTGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTCTGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCTTGGCCTTGGAGGGTGTCCCTATTTTTGGACTCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTCGATGTGATGTACGAGAACAAGGGCAGACTGATGTGTACAGCTGAGGGGACTCCATTTGAACTTTTTGAAAGAATAGTGACAATCTCTGATGCCCAACAAGTGGCACCTAGAACCTCTTCGAGATCAAGGAAAAATGATGATTCGGAGCTATGTGTAGATAATGAATTGGGATTTGCTAAAGACCGCACCATTAGTAGATTAACAGAAATGAATAGCAAAGAGTACTTGGAGCATCATGCTGCAGCGCTGGAAGAGAACGACTCCAATGAAGATCTGAATCAGGATAAAACCGTGCAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

312

Amino Acids

35.12

Weight (kDa)

4.85

Isoelectric Point (pI)

29.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 1 - 236 4.2e-64 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 739
AccB7I CCANNNNNTGG 2 cut(s) 565, 736
AccI GTMKAC 1 cut(s) 309
AciI CCGC 1 cut(s) 820
AclWI GGATC 1 cut(s) 249
AcsI RAATTY 1 cut(s) 107
AcuI CTGAAG 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 470
AfaI GTAC 3 cut(s) 647, 673, 860
AfeI AGCGCT 1 cut(s) 882
AfiI CCNNNNNNNGG 3 cut(s) 565, 590, 736
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 5 cut(s) 7, 56, 392, 695, 704
AjnI CCWGG 1 cut(s) 33
AleI CACNNNNGTG 1 cut(s) 36
AluBI AGCT 7 cut(s) 18, 163, 332, 416, 527, 677, 784
AluI AGCT 7 cut(s) 18, 163, 332, 416, 527, 677, 784
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 1 cut(s) 249
Aor51HI AGCGCT 1 cut(s) 882
AoxI GGCC 1 cut(s) 567
ApeKI GCWGC 6 cut(s) 18, 263, 527, 611, 875, 878
ApoI RAATTY 1 cut(s) 107
AseI ATTAAT 1 cut(s) 137
Asp700I GAANNNNTTC 2 cut(s) 387, 751
AspLEI GCGC 1 cut(s) 883
AsuHPI GGTGA 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 423
BanI GGYRCC 1 cut(s) 739
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 208
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 6 cut(s) 5, 250, 514, 623, 862, 890
BciT130I CCWGG 1 cut(s) 35
BfaI CTAG 2 cut(s) 467, 744
BfmI CTRYAG 2 cut(s) 528, 876
BfoI RGCGCY 1 cut(s) 884
BfrI CTTAAG 1 cut(s) 377
BglII AGATCT 1 cut(s) 907
BisI GCNGC 6 cut(s) 19, 264, 528, 612, 876, 879
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 6 cut(s) 20, 265, 529, 613, 877, 880
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 1 cut(s) 35
BmiI GGNNCC 2 cut(s) 462, 741
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 3 cut(s) 121, 715, 877
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 881, 913
BpmI CTGGAG 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 678
Bpu1102I GCTNAGC 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 3 cut(s) 34, 564, 570
Bsc4I CCNNNNNNNGG 3 cut(s) 565, 590, 736
Bse21I CCTNAGG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 34, 564, 570
BseGI GGATG 2 cut(s) 418, 485
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 565, 590, 736
BseMII CTCAG 3 cut(s) 324, 414, 669
BseRI GAGGAG 1 cut(s) 440
BseXI GCAGC 6 cut(s) 5, 250, 514, 623, 862, 890
BshFI GGCC 1 cut(s) 569
BshNI GGYRCC 1 cut(s) 739
BsiHKAI GWGCWC 1 cut(s) 208
BslFI GGGAC 2 cut(s) 566, 697
BslI CCNNNNNNNGG 3 cut(s) 565, 590, 736
BsmFI GGGAC 2 cut(s) 566, 697
BsnI GGCC 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 208
Bsp1407I TGTACA 1 cut(s) 671
Bsp143I GATC 3 cut(s) 241, 758, 907
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 1 cut(s) 820
BspANI GGCC 1 cut(s) 569
BspCNI CTCAG 3 cut(s) 325, 415, 670
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 2 cut(s) 462, 741
BspMAI CTGCAG 2 cut(s) 532, 880
BspPI GGATC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 739
BspTI CTTAAG 1 cut(s) 377
BsrGI TGTACA 1 cut(s) 671
BssECI CCNNGG 3 cut(s) 34, 564, 570
BssMI GATC 3 cut(s) 241, 758, 907
BssT1I CCWWGG 2 cut(s) 564, 570
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 928
Bst6I CTCTTC 2 cut(s) 757, 882
BstAFI CTTAAG 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 671
BstC8I GCNNGC 2 cut(s) 23, 933
BstDEI CTNAG 3 cut(s) 333, 423, 678
BstF5I GGATG 2 cut(s) 418, 485
BstH2I RGCGCY 1 cut(s) 884
BstHHI GCGC 1 cut(s) 883
BstKTI GATC 3 cut(s) 244, 761, 910
BstMBI GATC 3 cut(s) 241, 758, 907
BstNI CCWGG 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 2 cut(s) 528, 876
BstV1I GCAGC 6 cut(s) 5, 250, 514, 623, 862, 890
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 241, 907
BstYI RGATCY 2 cut(s) 241, 907
Bsu36I CCTNAGG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 569
BtsCI GGATG 2 cut(s) 418, 485
Cac8I GCNNGC 2 cut(s) 23, 933
CciI TCATGA 1 cut(s) 289
CfoI GCGC 1 cut(s) 883
Csp6I GTAC 3 cut(s) 646, 672, 859
CviAII CATG 4 cut(s) 112, 290, 872, 936
CviJI RGCY 9 cut(s) 18, 163, 332, 372, 416, 527, 569, 677, 784
CviKI_1 RGCY 9 cut(s) 18, 163, 332, 372, 416, 527, 569, 677, 784
CviQI GTAC 3 cut(s) 646, 672, 859
DdeI CTNAG 3 cut(s) 333, 423, 678
DpnI GATC 3 cut(s) 243, 760, 909
DpnII GATC 3 cut(s) 241, 758, 907
DraI TTTAAA 1 cut(s) 270
DraIII CACNNNGTG 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 757, 882
EarI CTCTTC 2 cut(s) 757, 882
Eco130I CCWWGG 2 cut(s) 564, 570
Eco32I GATATC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 882
Eco57I CTGAAG 1 cut(s) 312
Eco81I CCTNAGG 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 33
EcoRV GATATC 1 cut(s) 75
EcoT14I CCWWGG 2 cut(s) 564, 570
EcoT22I ATGCAT 1 cut(s) 136
ErhI CCWWGG 2 cut(s) 564, 570
FaeI CATG 4 cut(s) 115, 293, 875, 939
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 2 cut(s) 566, 697
FatI CATG 4 cut(s) 111, 289, 871, 935
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 19, 264, 528, 612, 876, 879
FokI GGATG 2 cut(s) 405, 492
Fsp4HI GCNGC 6 cut(s) 19, 264, 528, 612, 876, 879
FspBI CTAG 2 cut(s) 467, 744
GlaI GCGC 1 cut(s) 882
GluI GCNGC 6 cut(s) 19, 264, 528, 612, 876, 879
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 884
HaeIII GGCC 1 cut(s) 569
HhaI GCGC 1 cut(s) 883
Hin1II CATG 4 cut(s) 115, 293, 875, 939
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HinfI GANTC 5 cut(s) 594, 685, 776, 896, 913
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 3 cut(s) 13, 310, 672
Hpy188I TCNGA 4 cut(s) 79, 724, 781, 912
Hpy188III TCNNGA 8 cut(s) 53, 101, 209, 290, 392, 422, 756, 917
Hpy8I GTNNAC 3 cut(s) 13, 310, 672
HpyCH4III ACNGT 1 cut(s) 928
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 8 cut(s) 65, 134, 235, 485, 530, 549, 878, 931
HpyF3I CTNAG 3 cut(s) 333, 423, 678
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 4 cut(s) 115, 293, 875, 939
HspAI GCGC 1 cut(s) 881
Kzo9I GATC 3 cut(s) 241, 758, 907
LmnI GCTCC 4 cut(s) 211, 524, 781, 865
LpnPI CCDG 9 cut(s) 20, 47, 51, 222, 230, 354, 435, 869, 902
Lsp1109I GCAGC 6 cut(s) 5, 250, 514, 623, 862, 890
LweI GCATC 3 cut(s) 121, 715, 877
MaeI CTAG 2 cut(s) 467, 744
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 4 cut(s) 115, 509, 625, 712
MalI GATC 3 cut(s) 243, 760, 909
MboI GATC 3 cut(s) 241, 758, 907
MboII GAAGA 5 cut(s) 56, 459, 744, 899, 917
MflI RGATCY 2 cut(s) 241, 907
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 4 cut(s) 107, 294, 395, 800
MlyI GAGTC 3 cut(s) 588, 679, 890
MnlI CCTC 9 cut(s) 60, 205, 231, 418, 421, 493, 568, 673, 760
Mph1103I ATGCAT 1 cut(s) 136
MroXI GAANNNNTTC 2 cut(s) 387, 751
MseI TTAA 5 cut(s) 137, 269, 378, 455, 836
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 3 cut(s) 18, 332, 677
MspCI CTTAAG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
NdeII GATC 3 cut(s) 241, 758, 907
NlaIII CATG 4 cut(s) 115, 293, 875, 939
NlaIV GGNNCC 2 cut(s) 462, 741
NmuCI GTSAC 3 cut(s) 115, 509, 712
NsiI ATGCAT 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 36
PagI TCATGA 1 cut(s) 289
PdmI GAANNNNTTC 2 cut(s) 387, 751
PfeI GAWTC 2 cut(s) 776, 913
PflMI CCANNNNNTGG 2 cut(s) 565, 736
PkrI GCNGC 6 cut(s) 20, 265, 529, 613, 877, 880
PleI GAGTC 3 cut(s) 588, 679, 890
PpsI GAGTC 3 cut(s) 588, 679, 890
PshBI ATTAAT 1 cut(s) 137
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 462, 741
PstI CTGCAG 2 cut(s) 532, 880
PsuI RGATCY 2 cut(s) 241, 907
PvuII CAGCTG 3 cut(s) 18, 332, 677
RsaI GTAC 3 cut(s) 647, 673, 860
RsaNI GTAC 3 cut(s) 646, 672, 859
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 5 cut(s) 137, 269, 378, 455, 836
SatI GCNGC 6 cut(s) 19, 264, 528, 612, 876, 879
Sau3AI GATC 3 cut(s) 241, 758, 907
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 3 cut(s) 588, 679, 890
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 3 cut(s) 121, 715, 877
SfcI CTRYAG 2 cut(s) 528, 876
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 4 cut(s) 107, 294, 395, 800
SsiI CCGC 1 cut(s) 820
SspMI CTAG 2 cut(s) 467, 744
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 2 cut(s) 564, 570
TaaI ACNGT 1 cut(s) 928
TaiI ACGT 1 cut(s) 229
TaqI TCGA 3 cut(s) 168, 636, 755
TasI AATT 4 cut(s) 107, 294, 395, 800
TatI WGTACW 2 cut(s) 671, 858
TfiI GAWTC 2 cut(s) 776, 913
Tru1I TTAA 5 cut(s) 137, 269, 378, 455, 836
Tru9I TTAA 5 cut(s) 137, 269, 378, 455, 836
TseFI GTSAC 3 cut(s) 115, 509, 712
TseI GCWGC 6 cut(s) 18, 263, 527, 611, 875, 878
Tsp45I GTSAC 3 cut(s) 115, 509, 712
TspDTI ATGAA 9 cut(s) 96, 100, 120, 237, 278, 306, 813, 860, 918
Van91I CCANNNNNTGG 2 cut(s) 565, 736
Vha464I CTTAAG 1 cut(s) 377
VspI ATTAAT 1 cut(s) 137
XapI RAATTY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 2 cut(s) 387, 751
XspI CTAG 2 cut(s) 467, 744
ZrmI AGTACT 1 cut(s) 860
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.