Rw7G028210

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001671
Physical Location & Seq
Reverse (-)
37974238 .. 37983537
9300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G028210.1

Sequence Viewer

Length: 1215 bp
ATGAGAGCAATTACTCGTTCTATCCAGCAATTTAGATCAGCTCTGCGGCGGCAAACATGTAATTACTCCACTGGTTTTGTGAGAAGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGAGTAGTTTTGTTATTGATTATCCATTTGTCTATATGCAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCAAACAACTACAATGCAATTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTGACTAATGTTGCTGATGCATTAATACTAAATCGTCTATTTGGACATCTATTCGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAATGTGGATTGCAGAGGGATCTGTTTCTGCCCTTTATTGCTACTTTGAAGCTTGGGTCTCGAATCCAATACAAGACATTAAACCGAGCGCGTGCATATGTGGTACATAAAAATTGGAGTTCGTATGCAAATTACTGTGGTAAGTTTCCATTTTTGGAAACCTACCGGCTTTTCTCTCTCCTCTCCCCCGACCCTTTCTTCCTCTTCGGTAATGATATGATAAACAAGGAATTTTCATCTTTGGACTTTTGCCCTGATATCTATGGTTTGCATGAAGCGCTATATTGGGAAGGAAGACAATCCAAACCAAGAAGGAGGAGCTTTGTCCACCACGCCATCATCCACTCTCTCTCTCTTTCTTCCCCTTCCCATTCCAATTCTGATTCTGATTCCAAGTCCTCCGACTCCTCCATCCGCATACAGAATCGCCTGCCGGATCGACACCCCCGAGAACATCTGGGAGAGAGAGAGGAAAGTGAGGCAGAAGGGAGAGAAAATAAGGAGAAAAAAAATAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

404

Amino Acids

46.14

Weight (kDa)

9.48

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 109 - 248 5.1e-43 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 170, 784
AciI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1108
AclWI GGATC 2 cut(s) 720, 1137
AcsI RAATTY 3 cut(s) 146, 172, 923
AfaI GTAC 1 cut(s) 798
AfeI AGCGCT 1 cut(s) 972
AfiI CCNNNNNNNGG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 6 cut(s) 209, 369, 448, 505, 554, 742
AjnI CCWGG 1 cut(s) 531
AleI CACNNNNGTG 1 cut(s) 534
AluBI AGCT 8 cut(s) 41, 94, 108, 122, 188, 516, 745, 1014
AluI AGCT 8 cut(s) 41, 94, 108, 122, 188, 516, 745, 1014
Alw21I GWGCWC 2 cut(s) 190, 679
Alw26I GTCTC 2 cut(s) 351, 756
AlwI GGATC 2 cut(s) 720, 1137
Ama87I CYCGRG 1 cut(s) 1140
Aor51HI AGCGCT 1 cut(s) 972
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 269, 516
ApoI RAATTY 3 cut(s) 146, 172, 923
AseI ATTAAT 1 cut(s) 608
AspLEI GCGC 2 cut(s) 784, 973
AspS9I GGNCC 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 1140
BanII GRGCYC 1 cut(s) 190
BbsI GAAGAC 3 cut(s) 357, 549, 994
Bbv12I GWGCWC 2 cut(s) 190, 679
BbvI GCAGC 2 cut(s) 256, 503
BccI CCATC 5 cut(s) 281, 330, 427, 1037, 1112
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 2 cut(s) 351, 756
BfoI RGCGCY 1 cut(s) 974
BisI GCNGC 4 cut(s) 47, 50, 270, 517
BlsI GCNGC 4 cut(s) 48, 51, 271, 518
Bme1390I CCNGG 1 cut(s) 533
BmeT110I CYCGRG 1 cut(s) 1140
BmgT120I GGNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 3 cut(s) 190, 214, 592
BpiI GAAGAC 3 cut(s) 357, 549, 994
BpmI CTGGAG 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 166
BsaI GGTCTC 2 cut(s) 351, 756
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse118I RCCGGY 1 cut(s) 858
Bse1I ACTGG 2 cut(s) 76, 421
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 3 cut(s) 354, 1032, 1104
BseLI CCNNNNNNNGG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 76, 421
BseRI GAGGAG 4 cut(s) 420, 863, 1024, 1090
BseXI GCAGC 2 cut(s) 256, 503
Bsh1236I CGCG 2 cut(s) 170, 784
BshFI GGCC 1 cut(s) 241
BsiHKAI GWGCWC 2 cut(s) 190, 679
BsiHKCI CYCGRG 1 cut(s) 1140
BsiSI CCGG 2 cut(s) 859, 1127
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 2 cut(s) 351, 756
BsnI GGCC 1 cut(s) 241
Bso31I GGTCTC 2 cut(s) 351, 756
BsoBI CYCGRG 1 cut(s) 1140
Bsp1286I GDGCHC 2 cut(s) 190, 679
Bsp143I GATC 3 cut(s) 35, 712, 1129
BspACI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1108
BspANI GGCC 1 cut(s) 241
BspFNI CGCG 2 cut(s) 170, 784
BspHI TCATGA 2 cut(s) 124, 490
BspPI GGATC 2 cut(s) 720, 1137
BspTNI GGTCTC 2 cut(s) 351, 756
BsrFI RCCGGY 1 cut(s) 858
BsrI ACTGG 2 cut(s) 76, 421
BssAI RCCGGY 1 cut(s) 858
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 3 cut(s) 35, 712, 1129
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 363, 830
Bst6I CTCTTC 1 cut(s) 902
BstC8I GCNNGC 5 cut(s) 229, 274, 521, 786, 1124
BstENI CCTNNNNNAGG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 354, 1032, 1104
BstFNI CGCG 2 cut(s) 170, 784
BstH2I RGCGCY 1 cut(s) 974
BstHHI GCGC 2 cut(s) 784, 973
BstKTI GATC 3 cut(s) 38, 715, 1132
BstMAI GTCTC 2 cut(s) 351, 756
BstMBI GATC 3 cut(s) 35, 712, 1129
BstMWI GCNNNNNNNGC 1 cut(s) 970
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 531
BstUI CGCG 2 cut(s) 170, 784
BstV1I GCAGC 2 cut(s) 256, 503
BstV2I GAAGAC 3 cut(s) 357, 549, 994
BstX2I RGATCY 1 cut(s) 712
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 1 cut(s) 712
BsuRI GGCC 1 cut(s) 241
BtsCI GGATG 3 cut(s) 354, 1032, 1104
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 69, 591
Cac8I GCNNGC 5 cut(s) 229, 274, 521, 786, 1124
CciI TCATGA 2 cut(s) 124, 490
CfoI GCGC 2 cut(s) 784, 973
Cfr10I RCCGGY 1 cut(s) 858
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 797
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 5 cut(s) 57, 125, 427, 491, 965
CviQI GTAC 1 cut(s) 797
DpnI GATC 3 cut(s) 37, 714, 1131
DpnII GATC 3 cut(s) 35, 712, 1129
Eam1104I CTCTTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 902
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 351, 756
Eco32I GATATC 2 cut(s) 573, 952
Eco47III AGCGCT 1 cut(s) 972
Eco53kI GAGCTC 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 1140
EcoICRI GAGCTC 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 2 cut(s) 573, 952
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 190
FaeI CATG 5 cut(s) 60, 128, 430, 494, 968
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FatI CATG 5 cut(s) 56, 124, 426, 490, 964
FauI CCCGC 2 cut(s) 175, 265
FauNDI CATATG 1 cut(s) 790
Fnu4HI GCNGC 4 cut(s) 47, 50, 270, 517
FokI GGATG 3 cut(s) 341, 1019, 1091
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 4 cut(s) 47, 50, 270, 517
GlaI GCGC 2 cut(s) 783, 972
GluI GCNGC 4 cut(s) 47, 50, 270, 517
GsuI CTGGAG 1 cut(s) 663
HaeII RGCGCY 1 cut(s) 974
HaeIII GGCC 1 cut(s) 241
HapII CCGG 2 cut(s) 859, 1127
HhaI GCGC 2 cut(s) 784, 973
Hin1II CATG 5 cut(s) 60, 128, 430, 494, 968
Hin6I GCGC 2 cut(s) 782, 971
HinP1I GCGC 2 cut(s) 782, 971
HindIII AAGCTT 2 cut(s) 92, 743
HinfI GANTC 6 cut(s) 481, 756, 1076, 1082, 1097, 1117
HpaII CCGG 2 cut(s) 859, 1127
Hpy166II GTNNAC 2 cut(s) 511, 1021
Hpy188I TCNGA 4 cut(s) 577, 1075, 1081, 1096
Hpy188III TCNNGA 6 cut(s) 125, 183, 491, 551, 680, 753
Hpy8I GTNNAC 2 cut(s) 511, 1021
HpyAV CCTTC 5 cut(s) 78, 977, 999, 1068, 1172
HpyCH4III ACNGT 2 cut(s) 363, 830
HpyCH4V TGCA 9 cut(s) 227, 327, 462, 563, 605, 706, 788, 821, 964
HpyF10VI GCNNNNNNNGC 1 cut(s) 970
Hsp92II CATG 5 cut(s) 60, 128, 430, 494, 968
HspAI GCGC 2 cut(s) 782, 971
Kzo9I GATC 3 cut(s) 35, 712, 1129
LmnI GCTCC 2 cut(s) 682, 1011
Lsp1109I GCAGC 2 cut(s) 256, 503
LweI GCATC 3 cut(s) 190, 214, 592
MaeIII GTNAC 2 cut(s) 211, 586
MalI GATC 3 cut(s) 37, 714, 1131
MboI GATC 3 cut(s) 35, 712, 1129
MboII GAAGA 7 cut(s) 273, 357, 554, 883, 889, 999, 1044
MfeI CAATTG 1 cut(s) 463
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 2 cut(s) 190, 679
MlyI GAGTC 1 cut(s) 1091
MmeI TCCRAC 1 cut(s) 1119
Mph1103I ATGCAT 1 cut(s) 607
MseI TTAA 3 cut(s) 384, 608, 773
MslI CAYNNNNRTG 3 cut(s) 431, 489, 534
MspA1I CMGCKG 1 cut(s) 516
MspI CCGG 2 cut(s) 859, 1127
MspR9I CCNGG 1 cut(s) 533
MunI CAATTG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 2 cut(s) 170, 784
MwoI GCNNNNNNNGC 1 cut(s) 970
NdeI CATATG 1 cut(s) 790
NdeII GATC 3 cut(s) 35, 712, 1129
NlaIII CATG 5 cut(s) 60, 128, 430, 494, 968
NmuCI GTSAC 1 cut(s) 586
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 534
PagI TCATGA 2 cut(s) 124, 490
PciI ACATGT 1 cut(s) 56
PcsI WCGNNNNNNNCGW 2 cut(s) 645, 1138
PfeI GAWTC 5 cut(s) 481, 756, 1076, 1082, 1117
PkrI GCNGC 4 cut(s) 48, 51, 271, 518
PleI GAGTC 1 cut(s) 1091
PpsI GAGTC 1 cut(s) 1091
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 516
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 3 cut(s) 431, 489, 534
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 3 cut(s) 384, 608, 773
SatI GCNGC 4 cut(s) 47, 50, 270, 517
Sau3AI GATC 3 cut(s) 35, 712, 1129
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 1091
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 2 cut(s) 190, 679
SfaNI GCATC 3 cut(s) 190, 214, 592
SmiMI CAYNNNNRTG 3 cut(s) 431, 489, 534
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SsiI CCGC 6 cut(s) 46, 49, 88, 168, 272, 1108
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 1 cut(s) 531
TaaI ACNGT 2 cut(s) 363, 830
TaqI TCGA 4 cut(s) 150, 639, 754, 1132
TauI GCSGC 2 cut(s) 49, 52
TfiI GAWTC 5 cut(s) 481, 756, 1076, 1082, 1117
Tru1I TTAA 3 cut(s) 384, 608, 773
Tru9I TTAA 3 cut(s) 384, 608, 773
TscAI CASTG 2 cut(s) 76, 591
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 269, 516
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 7 cut(s) 141, 343, 473, 479, 708, 918, 981
TspRI CASTG 2 cut(s) 76, 591
VspI ATTAAT 1 cut(s) 608
XagI CCTNNNNNAGG 1 cut(s) 384
XapI RAATTY 3 cut(s) 146, 172, 923
XceI RCATGY 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.