RLG00000008864

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001671
Physical Location & Seq
Forward (+)
41402662 .. 41408423
5762 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008864

Sequence Viewer

Length: 1653 bp
ATGTTATTGCCATTGCCATCACAACAAGTCAAAACAGCACAGAGGCCAAAGCAAAACCAACTCATCATCACAACAACAAGAGCTGTGTTTTCTGGGTCTTCCCCTCACACCTCTGTTTCTGTCTTCTTCGAGATTAAGGATTGCGATTACTATATAAACCACGTTGGAGTGCTTGAGATTTGCATTGGAACATGTAATTACTCCAGTGGTTTTGTGAGAAGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCCCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAACGAAGTGGCAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAGACACCTTAAGAGAACTTCAAAGGCTATATGATGACCTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAGCTTCGAGGAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGCCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACCATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAGTTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATATCTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATACTAAATCGTCTATTTAGACATCTATTCGACAATGGCGTTATTCTTGTTGCCACCTCCAATCGTGCTCCAGATAACCTGTATGAACGTGGATTGCAGAGGGATTTGTTTCTGCCCTTTATTGCAGCTTTAAAGGAAAGATGTGTGGTTCATGAAATTGGTTCAGCAGTAGACTACCGAAAACTGACTTCGGCTGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCAGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTGCGAAGCTCATCCTGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTATGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCTTGGCCTTGGAGGGTGTCCCTATTTTTGGACTCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTACGAGAACAAGGGCAGACTGATGTGTACAGCTGAGGGGACTCCATTTGAACTCTTTGAAAGAATAGTGACAATCTCTGATGCCCAACAAGTGGCTCCTAGAACCTCTTCAAGATCAAGGAAAAATGATGATTCGGAGCTATGTGTAGATAATGAATTGGGATTTGCTAAAGACCGCACCATTAGTAGATTAACAGAAATGAATAGCAAAGAGTACTTGGAGCATCATGCTGCAGCGCTGGAAGAGAACGACTCCCATGAAGATCTGAATCAGGATAAAACAGTACACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

551

Amino Acids

61.87

Weight (kDa)

5.83

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 129 - 474 1.5e-89 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1279, 1450
AccI GTMKAC 2 cut(s) 447, 1023
AccII CGCG 1 cut(s) 305
AciI CCGC 4 cut(s) 223, 303, 413, 1534
AcsI RAATTY 3 cut(s) 281, 307, 821
AdeI CACNNNGTG 1 cut(s) 1184
AfaI GTAC 4 cut(s) 1361, 1387, 1574, 1644
AfeI AGCGCT 1 cut(s) 1596
AfiI CCNNNNNNNGG 4 cut(s) 602, 1279, 1304, 1450
AflII CTTAAG 2 cut(s) 454, 1091
AflIII ACRYGT 1 cut(s) 191
AgsI TTSAA 9 cut(s) 344, 467, 585, 721, 770, 1106, 1409, 1418, 1470
AjnI CCWGG 2 cut(s) 441, 747
AleI CACNNNNGTG 1 cut(s) 750
Alw21I GWGCWC 2 cut(s) 325, 922
Alw26I GTCTC 1 cut(s) 567
Aor51HI AGCGCT 1 cut(s) 1596
AoxI GGCC 4 cut(s) 44, 380, 604, 1281
ApeKI GCWGC 7 cut(s) 410, 732, 977, 1241, 1325, 1589, 1592
ApoI RAATTY 3 cut(s) 281, 307, 821
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AseI ATTAAT 1 cut(s) 851
Asp700I GAANNNNTTC 2 cut(s) 1101, 1465
AspLEI GCGC 1 cut(s) 1597
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 443, 841
AxyI CCTNAGG 1 cut(s) 1137
BanII GRGCYC 1 cut(s) 325
BbsI GAAGAC 4 cut(s) 90, 115, 573, 765
Bbv12I GWGCWC 2 cut(s) 325, 922
BbvCI CCTCAGC 1 cut(s) 1392
BbvI GCAGC 7 cut(s) 397, 719, 989, 1228, 1337, 1576, 1604
BccI CCATC 4 cut(s) 25, 422, 546, 643
BciT130I CCWGG 2 cut(s) 443, 749
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 2 cut(s) 1181, 1458
BfmI CTRYAG 2 cut(s) 1242, 1590
BfoI RGCGCY 1 cut(s) 1598
BfrI CTTAAG 2 cut(s) 454, 1091
BglII AGATCT 1 cut(s) 1621
BisI GCNGC 7 cut(s) 411, 733, 978, 1242, 1326, 1590, 1593
BlpI GCTNAGC 1 cut(s) 1047
BlsI GCNGC 7 cut(s) 412, 734, 979, 1243, 1327, 1591, 1594
BmcAI AGTACT 1 cut(s) 1574
Bme1390I CCNGG 2 cut(s) 443, 749
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 2 cut(s) 1176, 1455
BmrFI CCNGG 2 cut(s) 443, 749
BmsI GCATC 7 cut(s) 325, 355, 424, 490, 835, 1429, 1591
BpiI GAAGAC 4 cut(s) 90, 115, 573, 765
BplI GAGNNNNNCTC 2 cut(s) 1595, 1627
BpmI CTGGAG 2 cut(s) 187, 906
Bpu10I CCTNAGC 1 cut(s) 1392
Bpu1102I GCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 2 cut(s) 194, 301
BsaBI GATNNNNATC 1 cut(s) 1626
BsaI GGTCTC 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 748, 1278, 1284
Bsc4I CCNNNNNNNGG 4 cut(s) 602, 1279, 1304, 1450
Bse118I RCCGGY 1 cut(s) 507
Bse1I ACTGG 2 cut(s) 204, 637
Bse21I CCTNAGG 1 cut(s) 1137
Bse3DI GCAATG 1 cut(s) 11
Bse8I GATNNNNATC 1 cut(s) 1626
BseBI CCWGG 2 cut(s) 443, 749
BseDI CCNNGG 3 cut(s) 748, 1278, 1284
BseGI GGATG 3 cut(s) 570, 1132, 1199
BseJI GATNNNNATC 1 cut(s) 1626
BseLI CCNNNNNNNGG 4 cut(s) 602, 1279, 1304, 1450
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 3 cut(s) 1038, 1128, 1383
BseNI ACTGG 2 cut(s) 204, 637
BseRI GAGGAG 2 cut(s) 636, 1154
BseXI GCAGC 7 cut(s) 397, 719, 989, 1228, 1337, 1576, 1604
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 4 cut(s) 46, 382, 606, 1283
BsiHKAI GWGCWC 2 cut(s) 325, 922
BsiSI CCGG 1 cut(s) 508
BslFI GGGAC 2 cut(s) 1280, 1411
BslI CCNNNNNNNGG 4 cut(s) 602, 1279, 1304, 1450
BsmAI GTCTC 1 cut(s) 567
BsmFI GGGAC 2 cut(s) 1280, 1411
BsnI GGCC 4 cut(s) 46, 382, 606, 1283
Bso31I GGTCTC 1 cut(s) 567
Bsp1286I GDGCHC 2 cut(s) 325, 922
Bsp1407I TGTACA 1 cut(s) 1385
Bsp143I GATC 3 cut(s) 664, 1472, 1621
Bsp1720I GCTNAGC 1 cut(s) 1047
BspACI CCGC 4 cut(s) 223, 303, 413, 1534
BspANI GGCC 4 cut(s) 46, 382, 606, 1283
BspCNI CTCAG 3 cut(s) 1039, 1129, 1384
BspFNI CGCG 1 cut(s) 305
BspHI TCATGA 3 cut(s) 259, 706, 1003
BspLI GGNNCC 2 cut(s) 1176, 1455
BspMAI CTGCAG 2 cut(s) 1246, 1594
BspTI CTTAAG 2 cut(s) 454, 1091
BspTNI GGTCTC 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 11
BsrFI RCCGGY 1 cut(s) 507
BsrGI TGTACA 1 cut(s) 1385
BsrI ACTGG 2 cut(s) 204, 637
BssAI RCCGGY 1 cut(s) 507
BssECI CCNNGG 3 cut(s) 748, 1278, 1284
BssMI GATC 3 cut(s) 664, 1472, 1621
BssT1I CCWWGG 2 cut(s) 1278, 1284
Bst2UI CCWGG 2 cut(s) 443, 749
Bst4CI ACNGT 2 cut(s) 579, 1642
Bst6I CTCTTC 2 cut(s) 1471, 1596
BstAFI CTTAAG 2 cut(s) 454, 1091
BstAUI TGTACA 1 cut(s) 1385
BstC8I GCNNGC 6 cut(s) 370, 415, 439, 505, 509, 737
BstDEI CTNAG 3 cut(s) 1047, 1137, 1392
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 3 cut(s) 570, 1132, 1199
BstFNI CGCG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 1598
BstHHI GCGC 1 cut(s) 1597
BstKTI GATC 3 cut(s) 667, 1475, 1624
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 664, 1472, 1621
BstNI CCWGG 2 cut(s) 443, 749
BstNSI RCATGY 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 441, 747
BstSFI CTRYAG 2 cut(s) 1242, 1590
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 7 cut(s) 397, 719, 989, 1228, 1337, 1576, 1604
BstV2I GAAGAC 4 cut(s) 90, 115, 573, 765
BstX2I RGATCY 1 cut(s) 1621
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 1621
Bsu36I CCTNAGG 1 cut(s) 1137
BsuRI GGCC 4 cut(s) 46, 382, 606, 1283
BtsCI GGATG 3 cut(s) 570, 1132, 1199
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 6 cut(s) 370, 415, 439, 505, 509, 737
CciI TCATGA 3 cut(s) 259, 706, 1003
CfoI GCGC 1 cut(s) 1597
Cfr10I RCCGGY 1 cut(s) 507
Cfr13I GGNCC 1 cut(s) 380
Csp6I GTAC 4 cut(s) 1360, 1386, 1573, 1643
CviAII CATG 9 cut(s) 192, 260, 643, 707, 826, 1004, 1586, 1616, 1650
CviQI GTAC 4 cut(s) 1360, 1386, 1573, 1643
DdeI CTNAG 3 cut(s) 1047, 1137, 1392
DpnI GATC 3 cut(s) 666, 1474, 1623
DpnII GATC 3 cut(s) 664, 1472, 1621
DraI TTTAAA 1 cut(s) 984
DraIII CACNNNGTG 1 cut(s) 1184
Eam1104I CTCTTC 2 cut(s) 1471, 1596
EarI CTCTTC 2 cut(s) 1471, 1596
EciI GGCGGA 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 323
Eco130I CCWWGG 2 cut(s) 1278, 1284
Eco147I AGGCCT 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 567
Eco32I GATATC 1 cut(s) 789
Eco47III AGCGCT 1 cut(s) 1596
Eco53kI GAGCTC 1 cut(s) 323
Eco81I CCTNAGG 1 cut(s) 1137
EcoICRI GAGCTC 1 cut(s) 323
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 821
EcoRII CCWGG 2 cut(s) 441, 747
EcoRV GATATC 1 cut(s) 789
EcoT14I CCWWGG 2 cut(s) 1278, 1284
EcoT22I ATGCAT 1 cut(s) 850
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 2 cut(s) 1278, 1284
FaeI CATG 9 cut(s) 195, 263, 646, 710, 829, 1007, 1589, 1619, 1653
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 2 cut(s) 1280, 1411
FatI CATG 9 cut(s) 191, 259, 642, 706, 825, 1003, 1585, 1615, 1649
FauI CCCGC 2 cut(s) 310, 406
FblI GTMKAC 2 cut(s) 447, 1023
Fnu4HI GCNGC 7 cut(s) 411, 733, 978, 1242, 1326, 1590, 1593
FokI GGATG 3 cut(s) 557, 1119, 1206
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 7 cut(s) 411, 733, 978, 1242, 1326, 1590, 1593
FspBI CTAG 2 cut(s) 1181, 1458
GlaI GCGC 1 cut(s) 1596
GluI GCNGC 7 cut(s) 411, 733, 978, 1242, 1326, 1590, 1593
GsuI CTGGAG 2 cut(s) 187, 906
HaeII RGCGCY 1 cut(s) 1598
HaeIII GGCC 4 cut(s) 46, 382, 606, 1283
HapII CCGG 1 cut(s) 508
HhaI GCGC 1 cut(s) 1597
Hin1II CATG 9 cut(s) 195, 263, 646, 710, 829, 1007, 1589, 1619, 1653
Hin6I GCGC 1 cut(s) 1595
HinP1I GCGC 1 cut(s) 1595
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 6 cut(s) 697, 1308, 1399, 1490, 1610, 1627
HpaII CCGG 1 cut(s) 508
HphI GGTGA 2 cut(s) 443, 841
Hpy166II GTNNAC 5 cut(s) 448, 727, 1024, 1386, 1645
Hpy188I TCNGA 4 cut(s) 793, 1438, 1495, 1626
Hpy8I GTNNAC 5 cut(s) 448, 727, 1024, 1386, 1645
HpyAV CCTTC 2 cut(s) 213, 537
HpyCH4III ACNGT 2 cut(s) 579, 1642
HpyCH4IV ACGT 2 cut(s) 162, 940
HpyF3I CTNAG 3 cut(s) 1047, 1137, 1392
HpySE526I ACGT 2 cut(s) 162, 940
Hsp92II CATG 9 cut(s) 195, 263, 646, 710, 829, 1007, 1589, 1619, 1653
HspAI GCGC 1 cut(s) 1595
KroI GCCGGC 1 cut(s) 507
KroNI GCCGGC 1 cut(s) 509
Kzo9I GATC 3 cut(s) 664, 1472, 1621
LmnI GCTCC 5 cut(s) 925, 1238, 1459, 1495, 1579
Lsp1109I GCAGC 7 cut(s) 397, 719, 989, 1228, 1337, 1576, 1604
LweI GCATC 7 cut(s) 325, 355, 424, 490, 835, 1429, 1591
MaeI CTAG 2 cut(s) 1181, 1458
MaeII ACGT 2 cut(s) 162, 940
MaeIII GTNAC 5 cut(s) 346, 829, 1223, 1339, 1426
MalI GATC 3 cut(s) 666, 1474, 1623
MboI GATC 3 cut(s) 664, 1472, 1621
MflI RGATCY 1 cut(s) 1621
MhlI GDGCHC 2 cut(s) 325, 922
MluCI AATT 8 cut(s) 196, 281, 307, 405, 821, 1008, 1109, 1514
MlyI GAGTC 3 cut(s) 1302, 1393, 1604
MmeI TCCRAC 2 cut(s) 145, 662
Mph1103I ATGCAT 1 cut(s) 850
MroNI GCCGGC 1 cut(s) 507
MroXI GAANNNNTTC 2 cut(s) 1101, 1465
MseI TTAA 8 cut(s) 135, 455, 600, 851, 983, 1092, 1169, 1550
MslI CAYNNNNRTG 3 cut(s) 647, 705, 750
MspA1I CMGCKG 3 cut(s) 497, 732, 1391
MspCI CTTAAG 2 cut(s) 454, 1091
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 2 cut(s) 443, 749
MvaI CCWGG 2 cut(s) 443, 749
MvnI CGCG 1 cut(s) 305
NaeI GCCGGC 1 cut(s) 509
NdeII GATC 3 cut(s) 664, 1472, 1621
NgoMIV GCCGGC 1 cut(s) 507
NlaIII CATG 9 cut(s) 195, 263, 646, 710, 829, 1007, 1589, 1619, 1653
NlaIV GGNNCC 2 cut(s) 1176, 1455
NmuCI GTSAC 3 cut(s) 829, 1223, 1426
NsiI ATGCAT 1 cut(s) 850
NspI RCATGY 1 cut(s) 195
OliI CACNNNNGTG 1 cut(s) 750
PagI TCATGA 3 cut(s) 259, 706, 1003
PceI AGGCCT 1 cut(s) 606
PciI ACATGT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 888
PdiI GCCGGC 1 cut(s) 509
PdmI GAANNNNTTC 2 cut(s) 1101, 1465
PfeI GAWTC 3 cut(s) 697, 1490, 1627
PflMI CCANNNNNTGG 2 cut(s) 1279, 1450
PkrI GCNGC 7 cut(s) 412, 734, 979, 1243, 1327, 1591, 1594
PleI GAGTC 3 cut(s) 1302, 1393, 1604
PpsI GAGTC 3 cut(s) 1302, 1393, 1604
PscI ACATGT 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 851
Psp124BI GAGCTC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 441, 747
PspGI CCWGG 2 cut(s) 441, 747
PspN4I GGNNCC 2 cut(s) 1176, 1455
PspPI GGNCC 1 cut(s) 380
PstI CTGCAG 2 cut(s) 1246, 1594
PsuI RGATCY 1 cut(s) 1621
PvuII CAGCTG 3 cut(s) 497, 732, 1391
RsaI GTAC 4 cut(s) 1361, 1387, 1574, 1644
RsaNI GTAC 4 cut(s) 1360, 1386, 1573, 1643
RseI CAYNNNNRTG 3 cut(s) 647, 705, 750
SacI GAGCTC 1 cut(s) 325
SaqAI TTAA 8 cut(s) 135, 455, 600, 851, 983, 1092, 1169, 1550
SatI GCNGC 7 cut(s) 411, 733, 978, 1242, 1326, 1590, 1593
Sau3AI GATC 3 cut(s) 664, 1472, 1621
Sau96I GGNCC 1 cut(s) 380
ScaI AGTACT 1 cut(s) 1574
SchI GAGTC 3 cut(s) 1302, 1393, 1604
ScrFI CCNGG 2 cut(s) 443, 749
SduI GDGCHC 2 cut(s) 325, 922
SfaNI GCATC 7 cut(s) 325, 355, 424, 490, 835, 1429, 1591
SfcI CTRYAG 2 cut(s) 1242, 1590
SmiMI CAYNNNNRTG 3 cut(s) 647, 705, 750
SmlI CTYRAG 4 cut(s) 173, 316, 454, 1091
SmoI CTYRAG 4 cut(s) 173, 316, 454, 1091
Sse9I AATT 8 cut(s) 196, 281, 307, 405, 821, 1008, 1109, 1514
SseBI AGGCCT 1 cut(s) 606
SsiI CCGC 4 cut(s) 223, 303, 413, 1534
SspMI CTAG 2 cut(s) 1181, 1458
SstI GAGCTC 1 cut(s) 325
StuI AGGCCT 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 441, 747
StyI CCWWGG 2 cut(s) 1278, 1284
TaaI ACNGT 2 cut(s) 579, 1642
TaiI ACGT 2 cut(s) 165, 943
TaqI TCGA 5 cut(s) 129, 285, 530, 663, 882
TasI AATT 8 cut(s) 196, 281, 307, 405, 821, 1008, 1109, 1514
TatI WGTACW 3 cut(s) 1385, 1572, 1642
TfiI GAWTC 3 cut(s) 697, 1490, 1627
Tru1I TTAA 8 cut(s) 135, 455, 600, 851, 983, 1092, 1169, 1550
Tru9I TTAA 8 cut(s) 135, 455, 600, 851, 983, 1092, 1169, 1550
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 3 cut(s) 829, 1223, 1426
TseI GCWGC 7 cut(s) 410, 732, 977, 1241, 1325, 1589, 1592
Tsp45I GTSAC 3 cut(s) 829, 1223, 1426
TspRI CASTG 1 cut(s) 211
Van91I CCANNNNNTGG 2 cut(s) 1279, 1450
Vha464I CTTAAG 2 cut(s) 454, 1091
VspI ATTAAT 1 cut(s) 851
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 3 cut(s) 281, 307, 821
XceI RCATGY 1 cut(s) 195
XmiI GTMKAC 2 cut(s) 447, 1023
XmnI GAANNNNTTC 2 cut(s) 1101, 1465
XspI CTAG 2 cut(s) 1181, 1458
ZrmI AGTACT 1 cut(s) 1574
Zsp2I ATGCAT 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.