AT4G30490

AFG1-like ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001671
Physical Location & Seq
Reverse (-)
14899034 .. 14902144
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30490.1

Sequence Viewer

Length: 1494 bp
ATGAGACCAATCATTAGGTTATCATCTCTCAGTAGAATTAGATGGGCTCTAAGGAACAATCAAGAACGTTATTCTTCAACTTTCTACTCCAAAAGCCGCAAACTTTTGATCGGTGTCAATCAAAATCAAGCTCTGCTGAATACAAACACTGATAATTCAAGTTTGTATTCAAGGTCAAGTATCTTTAGAGGCTTATCAGCAGAAGCAGTAGAAGCAGCTGATCCTGCTACTACTAGGGTCACCGTCTCAGATGTGAATAGAACAGGTCCTCTTGTTGAGTATGAGAGAAGAATCAGTAATGGTGAACTCATGACTGGTGATATCTGCCAAATTAGTGCATTGAGAGAGCTTCAGAGACTATATGATGAGCTTGTGGATTCAGTAGATACATGTCGTTTAGATCGGTATAATACTTCTGATAAATCCTCAAGAAGTCGTTGGTTTTGGTCTCGGCTCATGCCACAGACTTCTTACTCACCCGTCAAAGGATTATACCTTTATGGAGGTGTAGGTACAGGAAAAACCATGTTGATGGACTTATTCTTTGATCAACTGCCTTGTACTTGGAAGAAGCAGAGGATACATTTCCATGATTTCATGTTGAGTGTTCATAGCCGCTTGCAAAAGCACAAGGGTTTATCAGATCCACTTGAAGTTGTTGCCCAAGAGATAGCTCATGATGCAATCTTACTATGTCTAGATGAGTTCATGGTAACTGATGTTGCAGATGCTCTAATACTAAACCGACTCTTTGGACATCTGTTTAGCAATGGTGTTATTCTTGTTGCTACATCGAATCGGAATCCTGATAAACTCTATGAAGGAGGACTCCAAAGAGATCTGTTCCTACCTTTCATTTCTTCATTGAAGGAACGAAGTGTGGTTCATGAGATCGGTTCAGCGGTTGATTACCGGAAACTAACTTCGGCTGAGCAAGGATTCTACTTCATTGGTAAAGATCTTTCGACCCTTTTGAAACAGAAGTTCCGACAATTGATTGGAGACAATGTTGTTGCCCGTCCACAGGTAGTAGAAGTGGTGATGGGAAGAAAATTACAGATTCCATTAGGTGCCAATGGATGTGCATACTTTCCTTTTGAAGAGCTATGTGACCGACCTCTTGGAGCTGCGGATTACTTCGGTTTGTTCAAGAAGTTTCATACTCTTGCCTTGGACGAAATTCCAGTGTTTGGGCTTCATAACCGTACTGCTGCATATAGATTCGTCACGCTAGTCGATGTAATGTATGAGAACAGAGCGAGGTTGTTGTGTACAGCTGAAGCAAATCCTCAAGAACTTTTGGAAAAGATTATAACAATCTCAGAGGCGAAATCGATGGGTCCAAGAACATCATCAAGATCAAGAAAGAACGATGTTACTGAGCTCTGCGTGGATAACGAATTAGGTTTCGCAAAAGACAGAACCATCAGTAGATTAACAGAAATGAACAGCAAAGAATACTTGGAGCACCATGCCATAACACACAATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

497

Amino Acids

56.53

Weight (kDa)

8.63

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 90 - 436 2.4e-87 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1313
AccB1I GGYRCC 1 cut(s) 1070
AccB7I CCANNNNNTGG 1 cut(s) 1190
AciI CCGC 4 cut(s) 97, 616, 902, 1130
AclI AACGTT 1 cut(s) 67
AclWI GGATC 2 cut(s) 215, 638
AcsI RAATTY 1 cut(s) 1179
AcuI CTGAAG 2 cut(s) 335, 1299
AfaI GTAC 4 cut(s) 514, 562, 1207, 1273
AfiI CCNNNNNNNGG 3 cut(s) 485, 1024, 1190
AflIII ACRYGT 1 cut(s) 389
AgsI TTSAA 8 cut(s) 78, 159, 171, 653, 868, 976, 1100, 1150
AluBI AGCT 9 cut(s) 131, 218, 349, 370, 674, 1105, 1127, 1277, 1384
AluI AGCT 9 cut(s) 131, 218, 349, 370, 674, 1105, 1127, 1277, 1384
Alw21I GWGCWC 2 cut(s) 1386, 1470
Alw26I GTCTC 4 cut(s) 250, 349, 453, 996
AlwI GGATC 2 cut(s) 215, 638
ApeKI GCWGC 3 cut(s) 215, 1127, 1211
ApoI RAATTY 1 cut(s) 1179
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AspS9I GGNCC 2 cut(s) 266, 1340
AsuHPI GGTGA 5 cut(s) 232, 314, 329, 468, 1051
AvaII GGWCC 2 cut(s) 266, 1340
BanI GGYRCC 1 cut(s) 1070
BanII GRGCYC 2 cut(s) 49, 1386
Bbv12I GWGCWC 2 cut(s) 1386, 1470
BbvI GCAGC 3 cut(s) 227, 1114, 1198
BccI CCATC 5 cut(s) 36, 526, 1036, 1330, 1433
BciVI GTATCC 1 cut(s) 573
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 4 cut(s) 250, 349, 453, 996
BfaI CTAG 3 cut(s) 234, 698, 1232
BfuI GTATCC 1 cut(s) 573
BglII AGATCT 2 cut(s) 838, 958
BisI GCNGC 5 cut(s) 97, 216, 616, 1128, 1212
BlpI GCTNAGC 1 cut(s) 930
BlsI GCNGC 5 cut(s) 98, 217, 617, 1129, 1213
Bme18I GGWCC 2 cut(s) 266, 1340
BmgT120I GGNCC 2 cut(s) 266, 1340
BmiI GGNNCC 2 cut(s) 1072, 1341
BmsI GCATC 2 cut(s) 670, 718
Bpu1102I GCTNAGC 1 cut(s) 930
BpuEI CTTGAG 2 cut(s) 412, 1275
Bsa29I ATCGAT 1 cut(s) 1334
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 1170
BsaWI WCCGGW 1 cut(s) 912
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 485, 1024, 1190
Bse1I ACTGG 2 cut(s) 319, 1184
Bse3DI GCAATG 1 cut(s) 775
BseCI ATCGAT 1 cut(s) 1334
BseDI CCNNGG 1 cut(s) 1170
BseGI GGATG 1 cut(s) 1085
BseLI CCNNNNNNNGG 3 cut(s) 485, 1024, 1190
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 5 cut(s) 43, 261, 921, 1335, 1371
BseNI ACTGG 2 cut(s) 319, 1184
BseXI GCAGC 3 cut(s) 227, 1114, 1198
BshNI GGYRCC 1 cut(s) 1070
BshVI ATCGAT 1 cut(s) 1334
BsiHKAI GWGCWC 2 cut(s) 1386, 1470
BsiSI CCGG 1 cut(s) 913
BslI CCNNNNNNNGG 3 cut(s) 485, 1024, 1190
BsmAI GTCTC 4 cut(s) 250, 349, 453, 996
BsmBI CGTCTC 1 cut(s) 250
Bso31I GGTCTC 1 cut(s) 453
Bsp1286I GDGCHC 3 cut(s) 49, 1386, 1470
Bsp1407I TGTACA 1 cut(s) 1271
Bsp143I GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
Bsp1720I GCTNAGC 1 cut(s) 930
BspACI CCGC 4 cut(s) 97, 616, 902, 1130
BspCNI CTCAG 5 cut(s) 42, 260, 922, 1334, 1372
BspDI ATCGAT 1 cut(s) 1334
BspHI TCATGA 3 cut(s) 309, 676, 886
BspLI GGNNCC 2 cut(s) 1072, 1341
BspPI GGATC 2 cut(s) 215, 638
BspQI GCTCTTC 1 cut(s) 1095
BspT107I GGYRCC 1 cut(s) 1070
BspTNI GGTCTC 1 cut(s) 453
BsrDI GCAATG 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 1271
BsrI ACTGG 2 cut(s) 319, 1184
BssECI CCNNGG 1 cut(s) 1170
BssMI GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
BssT1I CCWWGG 1 cut(s) 1170
Bst4CI ACNGT 2 cut(s) 244, 1205
Bst6I CTCTTC 1 cut(s) 1095
BstAUI TGTACA 1 cut(s) 1271
BstC8I GCNNGC 1 cut(s) 620
BstDEI CTNAG 6 cut(s) 29, 50, 247, 930, 1321, 1380
BstEII GGTNACC 1 cut(s) 238
BstF5I GGATG 1 cut(s) 1085
BstKTI GATC 9 cut(s) 111, 223, 403, 550, 646, 841, 894, 961, 1361
BstMAI GTCTC 4 cut(s) 250, 349, 453, 996
BstMBI GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
BstMWI GCNNNNNNNGC 3 cut(s) 212, 224, 680
BstNSI RCATGY 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 238
BstV1I GCAGC 3 cut(s) 227, 1114, 1198
BstX2I RGATCY 3 cut(s) 643, 838, 958
BstXI CCANNNNNNTGG 1 cut(s) 532
BstYI RGATCY 3 cut(s) 643, 838, 958
Bsu15I ATCGAT 1 cut(s) 1334
BsuI GTATCC 1 cut(s) 573
BsuTUI ATCGAT 1 cut(s) 1334
BtsCI GGATG 1 cut(s) 1085
BtsIMutI CAGTG 2 cut(s) 147, 1191
Cac8I GCNNGC 1 cut(s) 620
CciI TCATGA 3 cut(s) 309, 676, 886
Cfr13I GGNCC 2 cut(s) 266, 1340
ClaI ATCGAT 1 cut(s) 1334
Csp6I GTAC 4 cut(s) 513, 561, 1206, 1272
CviQI GTAC 4 cut(s) 513, 561, 1206, 1272
DdeI CTNAG 6 cut(s) 29, 50, 247, 930, 1321, 1380
DpnI GATC 9 cut(s) 110, 222, 402, 549, 645, 840, 893, 960, 1360
DpnII GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
Ecl136II GAGCTC 1 cut(s) 1384
Eco130I CCWWGG 1 cut(s) 1170
Eco24I GRGCYC 2 cut(s) 49, 1386
Eco31I GGTCTC 1 cut(s) 453
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 266, 1340
Eco53kI GAGCTC 1 cut(s) 1384
Eco57I CTGAAG 2 cut(s) 335, 1299
Eco91I GGTNACC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 1384
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 238
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 1170
EcoT38I GRGCYC 2 cut(s) 49, 1386
ErhI CCWWGG 1 cut(s) 1170
Esp3I CGTCTC 1 cut(s) 250
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 5 cut(s) 97, 216, 616, 1128, 1212
FokI GGATG 1 cut(s) 1092
FriOI GRGCYC 2 cut(s) 49, 1386
Fsp4HI GCNGC 5 cut(s) 97, 216, 616, 1128, 1212
FspBI CTAG 3 cut(s) 234, 698, 1232
GluI GCNGC 5 cut(s) 97, 216, 616, 1128, 1212
HapII CCGG 1 cut(s) 913
HinfI GANTC 9 cut(s) 291, 377, 747, 796, 802, 828, 939, 1060, 1221
HpaII CCGG 1 cut(s) 913
HphI GGTGA 5 cut(s) 232, 314, 329, 468, 1051
Hpy166II GTNNAC 3 cut(s) 305, 1022, 1272
Hpy188I TCNGA 7 cut(s) 250, 354, 418, 643, 801, 989, 1324
Hpy8I GTNNAC 3 cut(s) 305, 1022, 1272
HpyAV CCTTC 2 cut(s) 815, 862
HpyCH4III ACNGT 2 cut(s) 244, 1205
HpyCH4IV ACGT 1 cut(s) 67
HpyCH4V TGCA 6 cut(s) 338, 622, 683, 725, 1085, 1214
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 224, 680
HpyF3I CTNAG 6 cut(s) 29, 50, 247, 930, 1321, 1380
HpySE526I ACGT 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
LguI GCTCTTC 1 cut(s) 1095
LmnI GCTCC 2 cut(s) 1124, 1465
LpnPI CCDG 8 cut(s) 237, 249, 300, 501, 819, 926, 1010, 1197
Lsp1109I GCAGC 3 cut(s) 227, 1114, 1198
LweI GCATC 2 cut(s) 670, 718
MaeI CTAG 3 cut(s) 234, 698, 1232
MaeII ACGT 1 cut(s) 67
MaeIII GTNAC 5 cut(s) 238, 712, 1109, 1225, 1375
MalI GATC 9 cut(s) 110, 222, 402, 549, 645, 840, 893, 960, 1360
MboI GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
MboII GAAGA 6 cut(s) 66, 300, 580, 852, 1059, 1112
MfeI CAATTG 1 cut(s) 992
MflI RGATCY 3 cut(s) 643, 838, 958
MhlI GDGCHC 3 cut(s) 49, 1386, 1470
MluCI AATT 7 cut(s) 36, 154, 330, 992, 1052, 1179, 1400
MlyI GAGTC 2 cut(s) 741, 822
MmeI TCCRAC 1 cut(s) 1012
MseI TTAA 1 cut(s) 1436
MslI CAYNNNNRTG 2 cut(s) 530, 588
MspA1I CMGCKG 3 cut(s) 218, 902, 1277
MspI CCGG 1 cut(s) 913
MunI CAATTG 1 cut(s) 992
MwoI GCNNNNNNNGC 3 cut(s) 212, 224, 680
NdeII GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
NlaIV GGNNCC 2 cut(s) 1072, 1341
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 3 cut(s) 238, 1109, 1225
NspI RCATGY 1 cut(s) 393
PagI TCATGA 3 cut(s) 309, 676, 886
PciI ACATGT 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 1095
PfeI GAWTC 7 cut(s) 291, 377, 796, 802, 939, 1060, 1221
PflMI CCANNNNNTGG 1 cut(s) 1190
PkrI GCNGC 5 cut(s) 98, 217, 617, 1129, 1213
PleI GAGTC 2 cut(s) 741, 822
PpsI GAGTC 2 cut(s) 741, 822
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 1 cut(s) 389
PsiI TTATAA 1 cut(s) 1313
Psp124BI GAGCTC 1 cut(s) 1386
Psp1406I AACGTT 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 266
PspEI GGTNACC 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 1072, 1341
PspPI GGNCC 2 cut(s) 266, 1340
PspPPI RGGWCCY 1 cut(s) 266
PsuI RGATCY 3 cut(s) 643, 838, 958
PvuII CAGCTG 2 cut(s) 218, 1277
RsaI GTAC 4 cut(s) 514, 562, 1207, 1273
RsaNI GTAC 4 cut(s) 513, 561, 1206, 1272
RseI CAYNNNNRTG 2 cut(s) 530, 588
SacI GAGCTC 1 cut(s) 1386
SapI GCTCTTC 1 cut(s) 1095
SaqAI TTAA 1 cut(s) 1436
SatI GCNGC 5 cut(s) 97, 216, 616, 1128, 1212
Sau3AI GATC 9 cut(s) 108, 220, 400, 547, 643, 838, 891, 958, 1358
Sau96I GGNCC 2 cut(s) 266, 1340
SchI GAGTC 2 cut(s) 741, 822
SduI GDGCHC 3 cut(s) 49, 1386, 1470
SfaNI GCATC 2 cut(s) 670, 718
SinI GGWCC 2 cut(s) 266, 1340
SmiMI CAYNNNNRTG 2 cut(s) 530, 588
SmlI CTYRAG 2 cut(s) 427, 1290
SmoI CTYRAG 2 cut(s) 427, 1290
Sse9I AATT 7 cut(s) 36, 154, 330, 992, 1052, 1179, 1400
SsiI CCGC 4 cut(s) 97, 616, 902, 1130
SspMI CTAG 3 cut(s) 234, 698, 1232
SstI GAGCTC 1 cut(s) 1386
StyI CCWWGG 1 cut(s) 1170
TaaI ACNGT 2 cut(s) 244, 1205
TaiI ACGT 1 cut(s) 70
TaqI TCGA 4 cut(s) 794, 965, 1236, 1334
TaqII GACCGA 1 cut(s) 1128
TasI AATT 7 cut(s) 36, 154, 330, 992, 1052, 1179, 1400
TatI WGTACW 2 cut(s) 560, 1271
TauI GCSGC 2 cut(s) 99, 618
TfiI GAWTC 7 cut(s) 291, 377, 796, 802, 939, 1060, 1221
Tru1I TTAA 1 cut(s) 1436
Tru9I TTAA 1 cut(s) 1436
TscAI CASTG 2 cut(s) 154, 1191
TseFI GTSAC 3 cut(s) 238, 1109, 1225
TseI GCWGC 3 cut(s) 215, 1127, 1211
Tsp45I GTSAC 3 cut(s) 238, 1109, 1225
TspRI CASTG 2 cut(s) 154, 1191
Van91I CCANNNNNTGG 1 cut(s) 1190
VpaK11BI GGWCC 2 cut(s) 266, 1340
XapI RAATTY 1 cut(s) 1179
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 1 cut(s) 393
XspI CTAG 3 cut(s) 234, 698, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.