Rmu_ssc0000255.1_g000022

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000255.1
OG0001671
Physical Location & Seq
Forward (+)
157549 .. 162115
4567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000255.1_g000022.1.cds

Sequence Viewer

Length: 1203 bp
atgacaaacggtggagtgggtgcattcttatcggtttacataagaaaaaaaattgaggaacataatagatatacaacttcgaggaaatctggaaggagtagatggttgtggtctcgtttcatcccacagtcttcaaatgcctcccctgttaaaggtctttatctttatggaggagtgggaactggaaaaaccatgttgatggacttgtttttcgatcaactacaatgcaattggaggaaaaagaggattcattttcatgattttatgttgaatgtccacaactgcttgcgaaagcaccagggtgtagaagaccctcttgaagttgttgcaggagagataactgatgaggcagtcttgttatgtcttgatgaattcatggtgactgatgttgctgatgcattaatactaaatcgtctatttagacatctattcgacaatggcgttattcttgttgccacctccaatcgtgctccagataacctgtatgaacgtggattgcagagggatctgtttctgccctttatcgctgctttgaaggaaagatgtgtggctcatgaaattggttcagcagttgactaccgaaaactgacttcggctgagcaagggttttactttgttggtaaagatttgtcaggctttcttaaggaaaagtttcaagaattgattggggattacgaagctcatcctgaggaggcagaagtagttatgggaagaacattaaaggttccactaggtgctaatggatgtgcatattttccttttgaggaactctgtgacagacctatgggagctgcagattattttggattgtgcaagaaataccataccttggccttggagggtgtccctatttttggactccacaataggacagcagcatatcggtttgttactttagttgatgtgatgtacgagaacaagggcagactgatgtgtacagctgaggggactccatttgaactttttgaaagaatagtgacaatctctgatgcccaacaagtggcacctagaacctcttcgagatcaaggaaaaatgatgattcggagctatgtgtagataatgaattgggatttgctaaagaccgcaccattagtagattaacagaaatgaatagcaaagagtacttggagcatcatgctgcagcgctggaagagaacaactcccatgaagatctgaatcaggataaaaccgtgcacgcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

400

Amino Acids

45.44

Weight (kDa)

5.99

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1003
AccB7I CCANNNNNTGG 2 cut(s) 829, 1000
AciI CCGC 1 cut(s) 1084
AclWI GGATC 1 cut(s) 513
AcsI RAATTY 1 cut(s) 371
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 3 cut(s) 911, 937, 1124
AfeI AGCGCT 1 cut(s) 1146
AfiI CCNNNNNNNGG 4 cut(s) 152, 829, 854, 1000
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 7 cut(s) 135, 271, 320, 535, 656, 959, 968
AjnI CCWGG 1 cut(s) 297
AleI CACNNNNGTG 1 cut(s) 300
AluBI AGCT 4 cut(s) 680, 791, 941, 1048
AluI AGCT 4 cut(s) 680, 791, 941, 1048
Alw21I GWGCWC 2 cut(s) 472, 1197
Alw26I GTCTC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 1193
AlwI GGATC 1 cut(s) 513
Aor51HI AGCGCT 1 cut(s) 1146
AoxI GGCC 1 cut(s) 831
ApaLI GTGCAC 1 cut(s) 1193
ApeKI GCWGC 5 cut(s) 527, 791, 875, 1139, 1142
ApoI RAATTY 1 cut(s) 371
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AseI ATTAAT 1 cut(s) 401
Asp700I GAANNNNTTC 2 cut(s) 651, 1015
AspLEI GCGC 1 cut(s) 1147
AsuHPI GGTGA 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 687
BaeGI GKGCMC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 1003
BbsI GAAGAC 2 cut(s) 123, 315
Bbv12I GWGCWC 2 cut(s) 472, 1197
BbvCI CCTCAGC 1 cut(s) 942
BbvI GCAGC 5 cut(s) 514, 778, 887, 1126, 1154
BccI CCATC 2 cut(s) 96, 193
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 117
BfaI CTAG 2 cut(s) 731, 1008
BfmI CTRYAG 2 cut(s) 792, 1140
BfoI RGCGCY 1 cut(s) 1148
BfrI CTTAAG 1 cut(s) 641
BglII AGATCT 1 cut(s) 1171
BisI GCNGC 5 cut(s) 528, 792, 876, 1140, 1143
BlpI GCTNAGC 1 cut(s) 597
BlsI GCNGC 5 cut(s) 529, 793, 877, 1141, 1144
BmcAI AGTACT 1 cut(s) 1124
Bme1390I CCNGG 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 726, 1005
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 3 cut(s) 385, 979, 1141
BpiI GAAGAC 2 cut(s) 123, 315
BplI GAGNNNNNCTC 2 cut(s) 1145, 1177
BpmI CTGGAG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 942
Bpu1102I GCTNAGC 1 cut(s) 597
BsaBI GATNNNNATC 1 cut(s) 1176
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 298, 828, 834
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 829, 854, 1000
Bse1I ACTGG 1 cut(s) 187
Bse21I CCTNAGG 1 cut(s) 687
Bse8I GATNNNNATC 1 cut(s) 1176
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 3 cut(s) 298, 828, 834
BseGI GGATG 3 cut(s) 120, 682, 749
BseJI GATNNNNATC 1 cut(s) 1176
BseLI CCNNNNNNNGG 4 cut(s) 152, 829, 854, 1000
BseMII CTCAG 3 cut(s) 588, 678, 933
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 186, 704
BseSI GKGCMC 1 cut(s) 1197
BseXI GCAGC 5 cut(s) 514, 778, 887, 1126, 1154
BshFI GGCC 1 cut(s) 833
BshNI GGYRCC 1 cut(s) 1003
BsiHKAI GWGCWC 2 cut(s) 472, 1197
BslFI GGGAC 2 cut(s) 830, 961
BslI CCNNNNNNNGG 4 cut(s) 152, 829, 854, 1000
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 830, 961
BsmI GAATGC 1 cut(s) 23
BsnI GGCC 1 cut(s) 833
Bso31I GGTCTC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 472, 1197
Bsp1407I TGTACA 1 cut(s) 935
Bsp143I GATC 4 cut(s) 214, 505, 1022, 1171
Bsp1720I GCTNAGC 1 cut(s) 597
BspACI CCGC 1 cut(s) 1084
BspANI GGCC 1 cut(s) 833
BspCNI CTCAG 3 cut(s) 589, 679, 934
BspHI TCATGA 2 cut(s) 256, 553
BspLI GGNNCC 2 cut(s) 726, 1005
BspMAI CTGCAG 2 cut(s) 796, 1144
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 1003
BspTI CTTAAG 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 935
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 298, 828, 834
BssMI GATC 4 cut(s) 214, 505, 1022, 1171
BssT1I CCWWGG 2 cut(s) 828, 834
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 3 cut(s) 11, 129, 1192
Bst6I CTCTTC 2 cut(s) 1021, 1146
BstAFI CTTAAG 1 cut(s) 641
BstAUI TGTACA 1 cut(s) 935
BstC8I GCNNGC 2 cut(s) 287, 1197
BstDEI CTNAG 3 cut(s) 597, 687, 942
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 120, 682, 749
BstH2I RGCGCY 1 cut(s) 1148
BstHHI GCGC 1 cut(s) 1147
BstKTI GATC 4 cut(s) 217, 508, 1025, 1174
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 4 cut(s) 214, 505, 1022, 1171
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 297
BstSFI CTRYAG 2 cut(s) 792, 1140
BstSLI GKGCMC 1 cut(s) 1197
BstV1I GCAGC 5 cut(s) 514, 778, 887, 1126, 1154
BstV2I GAAGAC 2 cut(s) 123, 315
BstX2I RGATCY 2 cut(s) 505, 1171
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 2 cut(s) 505, 1171
Bsu36I CCTNAGG 1 cut(s) 687
BsuRI GGCC 1 cut(s) 833
BtsCI GGATG 3 cut(s) 120, 682, 749
Cac8I GCNNGC 2 cut(s) 287, 1197
CciI TCATGA 2 cut(s) 256, 553
CfoI GCGC 1 cut(s) 1147
Csp6I GTAC 3 cut(s) 910, 936, 1123
CviAII CATG 7 cut(s) 193, 257, 376, 554, 1136, 1166, 1200
CviJI RGCY 8 cut(s) 551, 596, 636, 680, 791, 833, 941, 1048
CviKI_1 RGCY 8 cut(s) 551, 596, 636, 680, 791, 833, 941, 1048
CviQI GTAC 3 cut(s) 910, 936, 1123
DdeI CTNAG 3 cut(s) 597, 687, 942
DpnI GATC 4 cut(s) 216, 507, 1024, 1173
DpnII GATC 4 cut(s) 214, 505, 1022, 1171
DraIII CACNNNGTG 1 cut(s) 734
Eam1104I CTCTTC 2 cut(s) 1021, 1146
EarI CTCTTC 2 cut(s) 1021, 1146
Eco130I CCWWGG 2 cut(s) 828, 834
Eco31I GGTCTC 1 cut(s) 117
Eco47III AGCGCT 1 cut(s) 1146
Eco81I CCTNAGG 1 cut(s) 687
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 828, 834
EcoT22I ATGCAT 1 cut(s) 400
ErhI CCWWGG 2 cut(s) 828, 834
FaeI CATG 7 cut(s) 196, 260, 379, 557, 1139, 1169, 1203
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 830, 961
FatI CATG 7 cut(s) 192, 256, 375, 553, 1135, 1165, 1199
Fnu4HI GCNGC 5 cut(s) 528, 792, 876, 1140, 1143
FokI GGATG 3 cut(s) 107, 669, 756
Fsp4HI GCNGC 5 cut(s) 528, 792, 876, 1140, 1143
FspBI CTAG 2 cut(s) 731, 1008
GlaI GCGC 1 cut(s) 1146
GluI GCNGC 5 cut(s) 528, 792, 876, 1140, 1143
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 1148
HaeIII GGCC 1 cut(s) 833
HhaI GCGC 1 cut(s) 1147
Hin1II CATG 7 cut(s) 196, 260, 379, 557, 1139, 1169, 1203
Hin6I GCGC 1 cut(s) 1145
HinP1I GCGC 1 cut(s) 1145
HincII GTYRAC 1 cut(s) 574
HindII GTYRAC 1 cut(s) 574
HinfI GANTC 5 cut(s) 247, 858, 949, 1040, 1177
HphI GGTGA 1 cut(s) 391
Hpy166II GTNNAC 5 cut(s) 37, 277, 574, 936, 1195
Hpy188I TCNGA 3 cut(s) 988, 1045, 1176
Hpy8I GTNNAC 5 cut(s) 37, 277, 574, 936, 1195
HpyAV CCTTC 2 cut(s) 87, 529
HpyCH4III ACNGT 3 cut(s) 11, 129, 1192
HpyCH4IV ACGT 1 cut(s) 490
HpyF3I CTNAG 3 cut(s) 597, 687, 942
HpySE526I ACGT 1 cut(s) 490
Hsp92II CATG 7 cut(s) 196, 260, 379, 557, 1139, 1169, 1203
HspAI GCGC 1 cut(s) 1145
Kzo9I GATC 4 cut(s) 214, 505, 1022, 1171
LmnI GCTCC 4 cut(s) 475, 788, 1045, 1129
Lsp1109I GCAGC 5 cut(s) 514, 778, 887, 1126, 1154
LweI GCATC 3 cut(s) 385, 979, 1141
MaeI CTAG 2 cut(s) 731, 1008
MaeII ACGT 1 cut(s) 490
MaeIII GTNAC 4 cut(s) 379, 773, 889, 976
MalI GATC 4 cut(s) 216, 507, 1024, 1173
MboI GATC 4 cut(s) 214, 505, 1022, 1171
MboII GAAGA 6 cut(s) 123, 320, 723, 1008, 1163, 1181
MfeI CAATTG 1 cut(s) 229
MflI RGATCY 2 cut(s) 505, 1171
MhlI GDGCHC 2 cut(s) 472, 1197
MluCI AATT 6 cut(s) 51, 229, 371, 558, 659, 1064
MlyI GAGTC 2 cut(s) 852, 943
Mph1103I ATGCAT 1 cut(s) 400
MroXI GAANNNNTTC 2 cut(s) 651, 1015
MseI TTAA 5 cut(s) 150, 401, 642, 719, 1100
MslI CAYNNNNRTG 3 cut(s) 197, 255, 300
MspA1I CMGCKG 1 cut(s) 941
MspCI CTTAAG 1 cut(s) 641
MspR9I CCNGG 1 cut(s) 299
MunI CAATTG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 23
MvaI CCWGG 1 cut(s) 299
NdeII GATC 4 cut(s) 214, 505, 1022, 1171
NlaIII CATG 7 cut(s) 196, 260, 379, 557, 1139, 1169, 1203
NlaIV GGNNCC 2 cut(s) 726, 1005
NmuCI GTSAC 3 cut(s) 379, 773, 976
NsiI ATGCAT 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 300
PagI TCATGA 2 cut(s) 256, 553
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PctI GAATGC 1 cut(s) 23
PdmI GAANNNNTTC 2 cut(s) 651, 1015
PfeI GAWTC 3 cut(s) 247, 1040, 1177
PflMI CCANNNNNTGG 2 cut(s) 829, 1000
PkrI GCNGC 5 cut(s) 529, 793, 877, 1141, 1144
PleI GAGTC 2 cut(s) 852, 943
PpsI GAGTC 2 cut(s) 852, 943
PshBI ATTAAT 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 726, 1005
PstI CTGCAG 2 cut(s) 796, 1144
PsuI RGATCY 2 cut(s) 505, 1171
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 3 cut(s) 911, 937, 1124
RsaNI GTAC 3 cut(s) 910, 936, 1123
RseI CAYNNNNRTG 3 cut(s) 197, 255, 300
SaqAI TTAA 5 cut(s) 150, 401, 642, 719, 1100
SatI GCNGC 5 cut(s) 528, 792, 876, 1140, 1143
Sau3AI GATC 4 cut(s) 214, 505, 1022, 1171
ScaI AGTACT 1 cut(s) 1124
SchI GAGTC 2 cut(s) 852, 943
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 2 cut(s) 472, 1197
SfaNI GCATC 3 cut(s) 385, 979, 1141
SfcI CTRYAG 2 cut(s) 792, 1140
SmiMI CAYNNNNRTG 3 cut(s) 197, 255, 300
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 6 cut(s) 51, 229, 371, 558, 659, 1064
SsiI CCGC 1 cut(s) 1084
SspMI CTAG 2 cut(s) 731, 1008
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 2 cut(s) 828, 834
TaaI ACNGT 3 cut(s) 11, 129, 1192
TaiI ACGT 1 cut(s) 493
TaqI TCGA 4 cut(s) 80, 213, 432, 1019
TasI AATT 6 cut(s) 51, 229, 371, 558, 659, 1064
TatI WGTACW 2 cut(s) 935, 1122
TfiI GAWTC 3 cut(s) 247, 1040, 1177
Tru1I TTAA 5 cut(s) 150, 401, 642, 719, 1100
Tru9I TTAA 5 cut(s) 150, 401, 642, 719, 1100
TseFI GTSAC 3 cut(s) 379, 773, 976
TseI GCWGC 5 cut(s) 527, 791, 875, 1139, 1142
Tsp45I GTSAC 3 cut(s) 379, 773, 976
Van91I CCANNNNNTGG 2 cut(s) 829, 1000
Vha464I CTTAAG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 1193
VspI ATTAAT 1 cut(s) 401
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 371
XmnI GAANNNNTTC 2 cut(s) 651, 1015
XspI CTAG 2 cut(s) 731, 1008
ZrmI AGTACT 1 cut(s) 1124
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.