RchiOBHm_Chr2g0167931

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001671
Physical Location & Seq
Forward (+)
82316988 .. 82319707
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53568

Sequence Viewer

Length: 681 bp
ATGAATATGCTTGATGCTTCTGGGTTTGTCATTCATGAATTAGGCAGTTTTGATCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCTCTAACAATGCCAAATTCGATAGATTTTCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTGATGCTGTTCAAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGTGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAAGCACATTAACAGAACTTCAAAGGCTATATGATGAACTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAACTTCGAGGAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTCTTTATCTTTATGGAGGAGTGGGAACTGGAAAAACTATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAATTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATAACTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGAGATGGCAGCTAAGAATCCGAAAAGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

226

Amino Acids

25.6

Weight (kDa)

5.99

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 69 - 215 7.9e-31 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 131
AciI CCGC 1 cut(s) 129
AcsI RAATTY 3 cut(s) 107, 133, 641
AfiI CCNNNNNNNGG 2 cut(s) 422, 673
AgsI TTSAA 5 cut(s) 170, 287, 405, 541, 590
AjnI CCWGG 2 cut(s) 261, 567
AleI CACNNNNGTG 1 cut(s) 570
AluBI AGCT 6 cut(s) 69, 83, 149, 317, 552, 658
AluI AGCT 6 cut(s) 69, 83, 149, 317, 552, 658
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 1 cut(s) 387
AoxI GGCC 1 cut(s) 200
ApeKI GCWGC 2 cut(s) 552, 655
ApoI RAATTY 3 cut(s) 107, 133, 641
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 393, 585
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 539, 667
BccI CCATC 4 cut(s) 242, 366, 463, 645
BciT130I CCWGG 2 cut(s) 263, 569
BcoDI GTCTC 1 cut(s) 387
BisI GCNGC 2 cut(s) 553, 656
BlsI GCNGC 2 cut(s) 554, 657
Bme1390I CCNGG 2 cut(s) 263, 569
BmgT120I GGNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 263, 569
BmsI GCATC 5 cut(s) 4, 151, 175, 244, 310
BpiI GAAGAC 2 cut(s) 393, 585
BpuEI CTTGAG 1 cut(s) 127
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 673
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 457
BseBI CCWGG 2 cut(s) 263, 569
BseDI CCNNGG 1 cut(s) 568
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 2 cut(s) 422, 673
BseNI ACTGG 1 cut(s) 457
BseRI GAGGAG 1 cut(s) 456
BseXI GCAGC 2 cut(s) 539, 667
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 422, 673
BsmAI GTCTC 1 cut(s) 387
BsnI GGCC 1 cut(s) 202
Bso31I GGTCTC 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 52, 484
BspACI CCGC 1 cut(s) 129
BspANI GGCC 1 cut(s) 202
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 4 cut(s) 34, 85, 526, 645
BspTNI GGTCTC 1 cut(s) 387
BsrFI RCCGGY 1 cut(s) 327
BsrI ACTGG 1 cut(s) 457
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 2 cut(s) 52, 484
Bst2UI CCWGG 2 cut(s) 263, 569
Bst4CI ACNGT 1 cut(s) 399
BstC8I GCNNGC 5 cut(s) 190, 259, 325, 329, 557
BstDEI CTNAG 1 cut(s) 659
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 131
BstKTI GATC 2 cut(s) 55, 487
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 2 cut(s) 52, 484
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 2 cut(s) 263, 569
BstSCI CCNGG 2 cut(s) 261, 567
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 539, 667
BstV2I GAAGAC 2 cut(s) 393, 585
BsuRI GGCC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 390
Cac8I GCNNGC 5 cut(s) 190, 259, 325, 329, 557
CciI TCATGA 4 cut(s) 34, 85, 526, 645
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 200
CviAII CATG 4 cut(s) 35, 86, 527, 646
DdeI CTNAG 1 cut(s) 659
DpnI GATC 2 cut(s) 54, 486
DpnII GATC 2 cut(s) 52, 484
Ecl136II GAGCTC 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 151
Eco31I GGTCTC 1 cut(s) 387
Eco53kI GAGCTC 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 149
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 261, 567
EcoT38I GRGCYC 1 cut(s) 151
FaeI CATG 4 cut(s) 38, 89, 530, 649
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FatI CATG 4 cut(s) 34, 85, 526, 645
FauI CCCGC 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 553, 656
FokI GGATG 1 cut(s) 377
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 2 cut(s) 553, 656
GluI GCNGC 2 cut(s) 553, 656
HaeIII GGCC 1 cut(s) 202
HapII CCGG 1 cut(s) 328
Hin1II CATG 4 cut(s) 38, 89, 530, 649
HinfI GANTC 2 cut(s) 517, 663
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 547
Hpy188I TCNGA 1 cut(s) 668
Hpy188III TCNNGA 8 cut(s) 35, 86, 144, 360, 527, 587, 635, 646
Hpy8I GTNNAC 1 cut(s) 547
HpyAV CCTTC 1 cut(s) 357
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 188, 498, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 1 cut(s) 659
Hsp92II CATG 4 cut(s) 38, 89, 530, 649
KroI GCCGGC 1 cut(s) 327
KroNI GCCGGC 1 cut(s) 329
Kzo9I GATC 2 cut(s) 52, 484
Lsp1109I GCAGC 2 cut(s) 539, 667
LweI GCATC 5 cut(s) 4, 151, 175, 244, 310
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 54, 486
MboI GATC 2 cut(s) 52, 484
MboII GAAGA 3 cut(s) 234, 393, 590
MfeI CAATTG 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 6 cut(s) 38, 107, 133, 225, 499, 641
MnlI CCTC 9 cut(s) 191, 345, 421, 434, 498, 507, 594, 610, 667
MroNI GCCGGC 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 2 cut(s) 275, 420
MslI CAYNNNNRTG 2 cut(s) 525, 570
MspA1I CMGCKG 2 cut(s) 317, 552
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 263, 569
MunI CAATTG 1 cut(s) 499
MvaI CCWGG 2 cut(s) 263, 569
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 267
NaeI GCCGGC 1 cut(s) 329
NdeII GATC 2 cut(s) 52, 484
NgoMIV GCCGGC 1 cut(s) 327
NlaIII CATG 4 cut(s) 38, 89, 530, 649
OliI CACNNNNGTG 1 cut(s) 570
PagI TCATGA 4 cut(s) 34, 85, 526, 645
PdiI GCCGGC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 517, 663
PkrI GCNGC 2 cut(s) 554, 657
Psp124BI GAGCTC 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 261, 567
PspGI CCWGG 2 cut(s) 261, 567
PspPI GGNCC 1 cut(s) 200
PvuII CAGCTG 2 cut(s) 317, 552
RseI CAYNNNNRTG 2 cut(s) 525, 570
SacI GAGCTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 275, 420
SatI GCNGC 2 cut(s) 553, 656
Sau3AI GATC 2 cut(s) 52, 484
Sau96I GGNCC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 263, 569
SduI GDGCHC 1 cut(s) 151
SetI ASST 8 cut(s) 71, 85, 151, 267, 319, 427, 554, 660
SfaNI GCATC 5 cut(s) 4, 151, 175, 244, 310
SmiMI CAYNNNNRTG 2 cut(s) 525, 570
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 6 cut(s) 38, 107, 133, 225, 499, 641
SsiI CCGC 1 cut(s) 129
SstI GAGCTC 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 261, 567
TaaI ACNGT 1 cut(s) 399
TaqI TCGA 3 cut(s) 111, 350, 483
TasI AATT 6 cut(s) 38, 107, 133, 225, 499, 641
TfiI GAWTC 2 cut(s) 517, 663
Tru1I TTAA 2 cut(s) 275, 420
Tru9I TTAA 2 cut(s) 275, 420
TseI GCWGC 2 cut(s) 552, 655
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 3 cut(s) 107, 133, 641
XmnI GAANNNNTTC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.