Rroxscaffold_6G00399190

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001671
Physical Location & Seq
Forward (+)
21093933 .. 21095383
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399190.1

Sequence Viewer

Length: 771 bp
ATGGCGTCGGCGTCGGCGTCGTCTTCGACTCTCTCAAGACTGTCCGGTATCGAGCGGACACTTCAATCCCTCCCACGCAAACAAGGACGAAATGGAGATGCAAACAAAGGAGGACCACTTGTTGAGTATGAGCGAAGAATTGCTGCAGGCGAACTTGTTGATGGTGATGCCTGCCGCTTGCGAAAGCACCGGGGTGTAGAAGACCCTCTTGAAGTTGTTGCGGGAGAGATATCGATGAGGCGCGATGAACTACGAAGACCGACTTCGGCCGAGCAAGGGTTTTACTTTGTTGGTAAAGATTTGTCGGGCTTTCTTAAGGAAAAGTTTCAAGAATTGATTGGGGATTACGAAGCTCATCCCGAGGAGGCAGAAGTAGTTATGGGAAGAACATTAAAGGTTCCACTAGGTGCTAATGGATGTGCATATTTTCCTTTTGAGGAACTGCGTGACAGACCTATGGGAGCTGCAGATTATTTTGGATTGTGCAAGAAATACCATACCTTGGCCTTGGAGGGTGTCCCTATTTTTGGACTCCACAATAGGACAGCAGCATATCGGTTTGTTACTTTAGTTGATGTGATGTACGAGAACAAGGGCGGTGATGTGTACAGTGAGGGGACTCCATTTGAACTTTTTGAAAGAATAGTGACAATCCACGATGCCCAACAAGTGGCACCTAGAACCTCTTCGAGATCAAGGAAAAATGATGATTCGGAGCTATGTGTAGATAATGAATTGGGGTTTGCTAAAGACCGCACCATTAGTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

256

Amino Acids

28.52

Weight (kDa)

5.85

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 84 - 204 4.1e-17 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccB7I CCANNNNNTGG 2 cut(s) 502, 670
AccBSI CCGCTC 1 cut(s) 55
AccII CGCG 1 cut(s) 243
AciI CCGC 5 cut(s) 55, 175, 221, 597, 754
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 3 cut(s) 5, 11, 17
AdeI CACNNNGTG 1 cut(s) 407
AfaI GTAC 2 cut(s) 584, 608
AfiI CCNNNNNNNGG 5 cut(s) 276, 502, 527, 670, 765
AflII CTTAAG 1 cut(s) 314
AgsI TTSAA 5 cut(s) 65, 212, 329, 629, 638
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 3 cut(s) 353, 464, 718
AluI AGCT 3 cut(s) 353, 464, 718
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 2 cut(s) 267, 504
ApeKI GCWGC 3 cut(s) 143, 464, 548
Asp700I GAANNNNTTC 2 cut(s) 324, 685
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 176, 611
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 113
BanI GGYRCC 1 cut(s) 673
BbsI GAAGAC 3 cut(s) 15, 207, 262
BbvI GCAGC 3 cut(s) 130, 451, 560
BccI CCATC 1 cut(s) 155
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 2 cut(s) 404, 678
BfmI CTRYAG 2 cut(s) 144, 465
BfrI CTTAAG 1 cut(s) 314
BisI GCNGC 4 cut(s) 144, 175, 465, 549
BlsI GCNGC 4 cut(s) 145, 176, 466, 550
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 113
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 2 cut(s) 399, 675
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 3 cut(s) 88, 157, 649
BpiI GAAGAC 3 cut(s) 15, 207, 262
BpuEI CTTGAG 1 cut(s) 19
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 233
BsaHI GRCGYC 3 cut(s) 5, 11, 17
BsaJI CCNNGG 4 cut(s) 190, 360, 501, 507
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 5 cut(s) 276, 502, 527, 670, 765
BseCI ATCGAT 1 cut(s) 233
BseDI CCNNGG 4 cut(s) 190, 360, 501, 507
BseGI GGATG 2 cut(s) 355, 422
BseLI CCNNNNNNNGG 5 cut(s) 276, 502, 527, 670, 765
BseRI GAGGAG 1 cut(s) 377
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 3 cut(s) 130, 451, 560
Bsh1236I CGCG 1 cut(s) 243
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 2 cut(s) 269, 506
BshNI GGYRCC 1 cut(s) 673
BshVI ATCGAT 1 cut(s) 233
BsiEI CGRYCG 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 2 cut(s) 45, 190
BslFI GGGAC 2 cut(s) 503, 631
BslI CCNNNNNNNGG 5 cut(s) 276, 502, 527, 670, 765
BsmFI GGGAC 2 cut(s) 503, 631
BsnI GGCC 2 cut(s) 269, 506
BsoBI CYCGRG 1 cut(s) 359
Bsp1407I TGTACA 1 cut(s) 606
Bsp143I GATC 1 cut(s) 692
BspACI CCGC 5 cut(s) 55, 175, 221, 597, 754
BspANI GGCC 2 cut(s) 269, 506
BspDI ATCGAT 1 cut(s) 233
BspFNI CGCG 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 399, 675
BspMAI CTGCAG 2 cut(s) 148, 469
BspT107I GGYRCC 1 cut(s) 673
BspTI CTTAAG 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 606
BssECI CCNNGG 4 cut(s) 190, 360, 501, 507
BssMI GATC 1 cut(s) 692
BssNI GRCGYC 3 cut(s) 5, 11, 17
BssT1I CCWWGG 2 cut(s) 501, 507
Bst4CI ACNGT 2 cut(s) 42, 611
Bst6I CTCTTC 1 cut(s) 691
BstACI GRCGYC 3 cut(s) 5, 11, 17
BstAFI CTTAAG 1 cut(s) 314
BstAUI TGTACA 1 cut(s) 606
BstC8I GCNNGC 3 cut(s) 148, 172, 179
BstF5I GGATG 2 cut(s) 355, 422
BstFNI CGCG 1 cut(s) 243
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 1 cut(s) 695
BstMBI GATC 1 cut(s) 692
BstMCI CGRYCG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 2 cut(s) 144, 465
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 3 cut(s) 130, 451, 560
BstV2I GAAGAC 3 cut(s) 15, 207, 262
BstZI CGGCCG 1 cut(s) 267
Bsu15I ATCGAT 1 cut(s) 233
BsuRI GGCC 2 cut(s) 269, 506
BsuTUI ATCGAT 1 cut(s) 233
BtgZI GCGATG 1 cut(s) 258
BtsCI GGATG 2 cut(s) 355, 422
BtsIMutI CAGTG 1 cut(s) 616
Cac8I GCNNGC 3 cut(s) 148, 172, 179
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 233
CseI GACGC 1 cut(s) 6
Csp6I GTAC 2 cut(s) 583, 607
CviJI RGCY 6 cut(s) 269, 309, 353, 464, 506, 718
CviKI_1 RGCY 6 cut(s) 269, 309, 353, 464, 506, 718
CviQI GTAC 2 cut(s) 583, 607
DpnI GATC 1 cut(s) 694
DpnII GATC 1 cut(s) 692
DraIII CACNNNGTG 1 cut(s) 407
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 691
EarI CTCTTC 1 cut(s) 691
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 501, 507
Eco32I GATATC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 113
Eco52I CGGCCG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 359
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 501, 507
ErhI CCWWGG 2 cut(s) 501, 507
FaiI YATR 7 cut(s) 129, 380, 424, 458, 498, 553, 721
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 247, 279
FaqI GGGAC 2 cut(s) 503, 631
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 144, 175, 465, 549
FokI GGATG 2 cut(s) 342, 429
Fsp4HI GCNGC 4 cut(s) 144, 175, 465, 549
FspBI CTAG 2 cut(s) 404, 678
GlaI GCGC 1 cut(s) 242
GluI GCNGC 4 cut(s) 144, 175, 465, 549
HaeIII GGCC 2 cut(s) 269, 506
HapII CCGG 2 cut(s) 45, 190
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 3 cut(s) 5, 11, 17
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 4 cut(s) 28, 531, 619, 710
HpaII CCGG 2 cut(s) 45, 190
HphI GGTGA 2 cut(s) 176, 611
Hpy166II GTNNAC 1 cut(s) 607
Hpy188I TCNGA 1 cut(s) 715
Hpy188III TCNNGA 5 cut(s) 36, 209, 329, 359, 690
Hpy8I GTNNAC 1 cut(s) 607
Hpy99I CGWCG 3 cut(s) 10, 16, 22
HpyCH4III ACNGT 2 cut(s) 42, 611
HpyCH4V TGCA 5 cut(s) 101, 146, 422, 467, 486
Hsp92I GRCGYC 3 cut(s) 5, 11, 17
HspAI GCGC 1 cut(s) 241
Kzo9I GATC 1 cut(s) 692
LmnI GCTCC 2 cut(s) 461, 715
LpnPI CCDG 4 cut(s) 58, 132, 184, 203
Lsp1109I GCAGC 3 cut(s) 130, 451, 560
LweI GCATC 3 cut(s) 88, 157, 649
MaeI CTAG 2 cut(s) 404, 678
MaeIII GTNAC 3 cut(s) 446, 562, 646
MalI GATC 1 cut(s) 694
MbiI CCGCTC 1 cut(s) 55
MboI GATC 1 cut(s) 692
MboII GAAGA 6 cut(s) 15, 147, 212, 267, 396, 678
MluCI AATT 3 cut(s) 138, 332, 734
MlyI GAGTC 3 cut(s) 22, 525, 613
MroXI GAANNNNTTC 2 cut(s) 324, 685
MseI TTAA 2 cut(s) 315, 392
MslI CAYNNNNRTG 1 cut(s) 192
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 2 cut(s) 45, 190
MspR9I CCNGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 243
NciI CCSGG 1 cut(s) 191
NdeII GATC 1 cut(s) 692
NlaIV GGNNCC 2 cut(s) 399, 675
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 2 cut(s) 446, 646
OliI CACNNNNGTG 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 2 cut(s) 324, 685
PfeI GAWTC 1 cut(s) 710
PflMI CCANNNNNTGG 2 cut(s) 502, 670
PkrI GCNGC 4 cut(s) 145, 176, 466, 550
PleI GAGTC 3 cut(s) 22, 525, 613
PpsI GAGTC 3 cut(s) 22, 525, 613
PspN4I GGNNCC 2 cut(s) 399, 675
PspPI GGNCC 1 cut(s) 113
PstI CTGCAG 2 cut(s) 148, 469
RsaI GTAC 2 cut(s) 584, 608
RsaNI GTAC 2 cut(s) 583, 607
RseI CAYNNNNRTG 1 cut(s) 192
SaqAI TTAA 2 cut(s) 315, 392
SatI GCNGC 4 cut(s) 144, 175, 465, 549
Sau3AI GATC 1 cut(s) 692
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 3 cut(s) 22, 525, 613
ScrFI CCNGG 1 cut(s) 191
SfaNI GCATC 3 cut(s) 88, 157, 649
SfcI CTRYAG 2 cut(s) 144, 465
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 1 cut(s) 192
SmlI CTYRAG 2 cut(s) 34, 314
SmoI CTYRAG 2 cut(s) 34, 314
Sse9I AATT 3 cut(s) 138, 332, 734
SsiI CCGC 5 cut(s) 55, 175, 221, 597, 754
SspMI CTAG 2 cut(s) 404, 678
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 501, 507
TaaI ACNGT 2 cut(s) 42, 611
TaqI TCGA 4 cut(s) 26, 51, 233, 689
TaqII GACCGA 1 cut(s) 274
TasI AATT 3 cut(s) 138, 332, 734
TatI WGTACW 1 cut(s) 606
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 2 cut(s) 315, 392
Tru9I TTAA 2 cut(s) 315, 392
TscAI CASTG 1 cut(s) 616
TseFI GTSAC 2 cut(s) 446, 646
TseI GCWGC 3 cut(s) 143, 464, 548
Tsp45I GTSAC 2 cut(s) 446, 646
TspDTI ATGAA 2 cut(s) 261, 747
TspRI CASTG 1 cut(s) 616
Van91I CCANNNNNTGG 2 cut(s) 502, 670
Vha464I CTTAAG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 113
XmnI GAANNNNTTC 2 cut(s) 324, 685
XspI CTAG 2 cut(s) 404, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.