Rh4DG239400

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001671
Physical Location & Seq
Reverse (-)
44672386 .. 44675801
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG239400.1

Sequence Viewer

Length: 684 bp
ATGAATATGCTTGATGCTTCTGGGCGGAAGCTTTTGACAAACAGCTATTCTGGGTTAGCTCATGAACTCTCTAACAATGCCAAATTCGATAGATTTCCACACCCCGCGAATTTTGGCTCAAGAGCTCTGTCAGTTACTAATGGAGATGCAAGCAAAGGAGGGCCACTTGTTGAGTATGAGCGAAGAATTGCTGCGGGCGAACTTGTTGATGGTGATGCCTGCCAGGTAAGCACCTTAACAGAACTTCAAAGGCTATATGATGAACTTGTTCAGTCAGCTGATGCCTGCCGGCTGGATAGATATACAACTTCGAGGAAATCTGGAAGGAGTAGATGGTTGTGGTCTCGTTTCATCCCACAGTCTTCAAATGCCTCCCCTGTTAAAGGTATTTATCTTTATGGAGGAGTGGGAACTGGAAAAACTATGTTGATGGACTTGTTTTTCGATCAACTACAATGCAATTGGAGGAAAAAGAGGATTCATTTTCATGATTTTATGTTGAATGTCCACAGCTGCTTGCGAAAGCACCAGGGTGTAGAAGACCCTCTTGAAGTTGTTGCAGGAGAGATAACTGATGAGGCAGTCTTGTTATGTCTTGATGAATTCATGGTGACTAATGTTGCTGATGCATTAATATTGAATGTACTAACTGCAGTTAGAACAAAGTTTTCTTTTCAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

227

Amino Acids

25.41

Weight (kDa)

7.0

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AFG1_ATPase PF03969 56 - 216 2e-35 AFG1-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 107
AciI CCGC 3 cut(s) 25, 105, 194
AcsI RAATTY 3 cut(s) 83, 109, 602
AfaI GTAC 1 cut(s) 645
AfiI CCNNNNNNNGG 1 cut(s) 383
AgsI TTSAA 5 cut(s) 248, 366, 502, 551, 640
AjnI CCWGG 2 cut(s) 222, 528
AleI CACNNNNGTG 1 cut(s) 531
AluBI AGCT 6 cut(s) 31, 45, 59, 125, 278, 513
AluI AGCT 6 cut(s) 31, 45, 59, 125, 278, 513
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 348
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 191, 513
ApoI RAATTY 3 cut(s) 83, 109, 602
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 632
Asp700I GAANNNNTTC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 224, 622
BanII GRGCYC 1 cut(s) 127
BbsI GAAGAC 2 cut(s) 354, 546
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 2 cut(s) 178, 500
BccI CCATC 3 cut(s) 203, 327, 424
BciT130I CCWGG 2 cut(s) 224, 530
BcoDI GTCTC 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 651
BisI GCNGC 2 cut(s) 192, 514
BlsI GCNGC 2 cut(s) 193, 515
Bme1390I CCNGG 2 cut(s) 224, 530
BmgT120I GGNCC 1 cut(s) 161
BmrFI CCNGG 2 cut(s) 224, 530
BmsI GCATC 5 cut(s) 4, 136, 205, 271, 616
BpiI GAAGAC 2 cut(s) 354, 546
BpuEI CTTGAG 1 cut(s) 103
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 529
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 418
BseBI CCWGG 2 cut(s) 224, 530
BseDI CCNNGG 1 cut(s) 529
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 418
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 2 cut(s) 178, 500
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 163
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 383
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 1 cut(s) 445
BspACI CCGC 3 cut(s) 25, 105, 194
BspANI GGCC 1 cut(s) 163
BspFNI CGCG 1 cut(s) 107
BspHI TCATGA 2 cut(s) 61, 487
BspMAI CTGCAG 1 cut(s) 655
BspTNI GGTCTC 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 529
BssMI GATC 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 224, 530
Bst4CI ACNGT 1 cut(s) 360
BstC8I GCNNGC 6 cut(s) 151, 196, 220, 286, 290, 518
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 351
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 1 cut(s) 448
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 1 cut(s) 445
BstMWI GCNNNNNNNGC 1 cut(s) 228
BstNI CCWGG 2 cut(s) 224, 530
BstSCI CCNGG 2 cut(s) 222, 528
BstSFI CTRYAG 1 cut(s) 651
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 178, 500
BstV2I GAAGAC 2 cut(s) 354, 546
BsuRI GGCC 1 cut(s) 163
BtsCI GGATG 1 cut(s) 351
Cac8I GCNNGC 6 cut(s) 151, 196, 220, 286, 290, 518
CciI TCATGA 2 cut(s) 61, 487
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 644
CviAII CATG 3 cut(s) 62, 488, 607
CviQI GTAC 1 cut(s) 644
DpnI GATC 1 cut(s) 447
DpnII GATC 1 cut(s) 445
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 125
Eco24I GRGCYC 1 cut(s) 127
Eco31I GGTCTC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 125
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 2 cut(s) 222, 528
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 127
FaeI CATG 3 cut(s) 65, 491, 610
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 3 cut(s) 61, 487, 606
FauI CCCGC 2 cut(s) 112, 187
Fnu4HI GCNGC 2 cut(s) 192, 514
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 2 cut(s) 192, 514
GluI GCNGC 2 cut(s) 192, 514
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 289
Hin1II CATG 3 cut(s) 65, 491, 610
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 478
HpaII CCGG 1 cut(s) 289
HphI GGTGA 2 cut(s) 224, 622
Hpy166II GTNNAC 1 cut(s) 508
Hpy188III TCNNGA 6 cut(s) 62, 120, 321, 488, 548, 596
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 1 cut(s) 360
HpyCH4V TGCA 5 cut(s) 149, 459, 560, 629, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 228
Hsp92II CATG 3 cut(s) 65, 491, 610
KroI GCCGGC 1 cut(s) 288
KroNI GCCGGC 1 cut(s) 290
Kzo9I GATC 1 cut(s) 445
Lsp1109I GCAGC 2 cut(s) 178, 500
LweI GCATC 5 cut(s) 4, 136, 205, 271, 616
MaeIII GTNAC 2 cut(s) 133, 610
MalI GATC 1 cut(s) 447
MboI GATC 1 cut(s) 445
MboII GAAGA 3 cut(s) 195, 354, 551
MfeI CAATTG 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 5 cut(s) 83, 109, 186, 460, 602
MnlI CCTC 8 cut(s) 152, 306, 382, 395, 459, 468, 555, 571
Mph1103I ATGCAT 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 288
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 3 cut(s) 236, 381, 632
MslI CAYNNNNRTG 2 cut(s) 486, 531
MspA1I CMGCKG 2 cut(s) 278, 513
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 2 cut(s) 224, 530
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 2 cut(s) 224, 530
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 228
NaeI GCCGGC 1 cut(s) 290
NdeII GATC 1 cut(s) 445
NgoMIV GCCGGC 1 cut(s) 288
NlaIII CATG 3 cut(s) 65, 491, 610
NmuCI GTSAC 1 cut(s) 610
NsiI ATGCAT 1 cut(s) 631
OliI CACNNNNGTG 1 cut(s) 531
PagI TCATGA 2 cut(s) 61, 487
PdiI GCCGGC 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 1 cut(s) 478
PkrI GCNGC 2 cut(s) 193, 515
PshBI ATTAAT 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 222, 528
PspGI CCWGG 2 cut(s) 222, 528
PspPI GGNCC 1 cut(s) 161
PstI CTGCAG 1 cut(s) 655
PvuII CAGCTG 2 cut(s) 278, 513
RsaI GTAC 1 cut(s) 645
RsaNI GTAC 1 cut(s) 644
RseI CAYNNNNRTG 2 cut(s) 486, 531
SacI GAGCTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 236, 381, 632
SatI GCNGC 2 cut(s) 192, 514
Sau3AI GATC 1 cut(s) 445
Sau96I GGNCC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 224, 530
SduI GDGCHC 1 cut(s) 127
SetI ASST 9 cut(s) 33, 47, 61, 127, 228, 236, 280, 388, 515
SfaNI GCATC 5 cut(s) 4, 136, 205, 271, 616
SfcI CTRYAG 1 cut(s) 651
SmiMI CAYNNNNRTG 2 cut(s) 486, 531
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 5 cut(s) 83, 109, 186, 460, 602
SsiI CCGC 3 cut(s) 25, 105, 194
SspI AATATT 1 cut(s) 636
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 2 cut(s) 222, 528
TaaI ACNGT 1 cut(s) 360
TaqI TCGA 3 cut(s) 87, 311, 444
TasI AATT 5 cut(s) 83, 109, 186, 460, 602
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 1 cut(s) 478
Tru1I TTAA 3 cut(s) 236, 381, 632
Tru9I TTAA 3 cut(s) 236, 381, 632
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 2 cut(s) 191, 513
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 8 cut(s) 17, 78, 276, 340, 470, 476, 595, 615
VspI ATTAAT 1 cut(s) 632
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 3 cut(s) 83, 109, 602
XmnI GAANNNNTTC 1 cut(s) 267
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.