pycom13g28650

Lactation elevated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001671
Physical Location & Seq
Forward (+)
24606201 .. 24611093
4893 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28650.13

Sequence Viewer

Length: 1509 bp
ATGAGAGCGATTGCTCAATCTATTCACCAATGTAGACTGTCTTTGCGGCATCAAGCATGTCATTACTCAAGTGGTTTTGTGACAAGGCAGAAGCCGTTGGTTTTTACCTGTGCTGGCGTTGCTCATCAATTCGCTAAAAATGGCGAGTTTGATATACTTCCACCCCCCACGCTTATGGTCTCAAGAGCTCTGTCAATTGATGCAGCTCAAGTTACTAATGGAGATGTAAAAAAAGCGGGGCCACTTGTTGAGTATGAACGAAGAATTGCTGCAGGGGAACTTGTTGACGGTGATGCCTACCAGGTAGGCACCTTAAGAGAACTCCAAAGGCTTTATGATGAACTTGTTCAATTAGCTGATGCCTGCCGGTTGGATAGATATTCAACTTCTGCAAAATCCGGAAGGAGTAGGTGGTTGTGGTCTCGTTTCATCCCACAATCTTCAACCTCACCTGTCAAAGGTCTTTATCTATATGGAGGAGTGGGAACCGGTAAAACCATGTTGATGGACTTGTTTTTTTATCAGCTACAATGCAGTTGGAGGAAAAAGAGGATTCATTTTCATGACTTTATGCTGAATGTACATAAAACATTGCGAAAGCACCAGGGTGTAGAAGATCCGCTTGAAGTTGTTGCAGGAGAGATATCGGACGAGGCAGTCTTGTTGTGTCTGGATGAATTCATGGTGACTGATGTTGCTGATGCATTAATATTAAATCGTCTGTTTAGACATCTCTTCGATAACGGTATTATCCTTGTTTCCACCTCCAATCGTGCTCCAGATAAGCTGTATGAAGGTGGACTGCAGAGGGATCTTTTTCTGCCGTTCATTGCGACCTTGAAGGAAAGATGTGTAGTTCATGAAATTGGTTCTGCAGTAGACTACCGGAAACTGACTTCGGCGGAGCAGGGTTTCTACTTTGTTGGCAAAGATTTGTCGGGATTTCTTGAGCAAAAATTTCAAGAATTGATTGGGGAACACGAAGCTGTTCCACAAGAAGCAGAAGTAGTAATGGGAAGGACATTAAAGGTTCCGCTAGGTGCTAACGGATGTGCGTATTTTCCTTTTGAGGAACTCTGTGACAGACCATTGGGAGCTGCAGATTATTTGGGATTGTGCAAGAACTACCATACCTTGGCATTGGAGGGCGTCCCAGTTTTCGGACTCCACAATAGGGCAGCTGCATACCGGTTTGTCACTCTAGTTGATGTGATGTACGAGAACAAGGCCAGGCTTTTGTGTACAGCTGAGGGAACTCCCTTTGAACTCTTTGAAAAGATAGTGACAATCGCTGATGCCCAACAAATTGCACCTAGAACCTCTTCAAGGTCAAGGAAAAATGATGTTGCAGGGCTGTGCGTGGATAATGAATTGGGTTTTGCCAAAGACCGCACCATTAGTAGATTAACAGAGATGAATAGCAAAGAATATTTGGAGCACCATGCCGAAACATTGGCTGAGAAGAATTCACAGGAAGGTGTGAATCTCAATAAGATGGTGCAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AFG1_ATPase

Protein Analysis

503

Amino Acids

56.33

Weight (kDa)

6.45

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 1135
AccI GTMKAC 2 cut(s) 34, 879
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 6 cut(s) 46, 236, 620, 902, 1034, 1390
AclWI GGATC 2 cut(s) 611, 819
AcsI RAATTY 3 cut(s) 677, 956, 1465
AcyI GRCGYC 1 cut(s) 1149
AfaI GTAC 3 cut(s) 582, 1217, 1243
AfiI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1174, 1326
AflII CTTAAG 1 cut(s) 313
AgeI ACCGGT 2 cut(s) 488, 1188
AgsI TTSAA 9 cut(s) 350, 384, 444, 626, 841, 962, 1265, 1274, 1326
AjnI CCWGG 3 cut(s) 300, 603, 1229
AleI CACNNNNGTG 1 cut(s) 606
AluBI AGCT 9 cut(s) 188, 206, 356, 526, 787, 986, 1097, 1181, 1247
AluI AGCT 9 cut(s) 188, 206, 356, 526, 787, 986, 1097, 1181, 1247
Alw21I GWGCWC 3 cut(s) 190, 778, 1440
Alw26I GTCTC 2 cut(s) 184, 426
AlwI GGATC 2 cut(s) 611, 819
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 2 cut(s) 239, 1227
ApeKI GCWGC 5 cut(s) 203, 269, 1097, 1178, 1181
ApoI RAATTY 3 cut(s) 677, 956, 1465
AseI ATTAAT 1 cut(s) 707
AsiGI ACCGGT 2 cut(s) 488, 1188
Asp700I GAANNNNTTC 3 cut(s) 345, 987, 1321
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 4 cut(s) 17, 302, 441, 697
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 190
Bbv12I GWGCWC 3 cut(s) 190, 778, 1440
BbvCI CCTCAGC 1 cut(s) 1248
BbvI GCAGC 5 cut(s) 215, 256, 1084, 1168, 1190
BccI CCATC 2 cut(s) 499, 1489
BceAI ACGGC 2 cut(s) 79, 808
BciT130I CCWGG 3 cut(s) 302, 605, 1231
BcoDI GTCTC 2 cut(s) 184, 426
BfaI CTAG 3 cut(s) 1037, 1202, 1314
BfmI CTRYAG 4 cut(s) 270, 803, 873, 1098
BfrI CTTAAG 1 cut(s) 313
BisI GCNGC 6 cut(s) 47, 204, 270, 1098, 1179, 1182
BlsI GCNGC 6 cut(s) 48, 205, 271, 1099, 1180, 1183
Bme1390I CCNGG 3 cut(s) 302, 605, 1231
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 4 cut(s) 240, 310, 487, 1032
BmrFI CCNGG 3 cut(s) 302, 605, 1231
BmrI ACTGGG 1 cut(s) 1148
BmsI GCATC 6 cut(s) 58, 190, 283, 349, 691, 1285
BmuI ACTGGG 1 cut(s) 1148
BpmI CTGGAG 1 cut(s) 762
Bpu10I CCTNAGC 1 cut(s) 1248
BpuEI CTTGAG 4 cut(s) 52, 166, 192, 968
BsaHI GRCGYC 1 cut(s) 1149
BsaI GGTCTC 2 cut(s) 184, 426
BsaJI CCNNGG 2 cut(s) 604, 1134
BsaWI WCCGGW 4 cut(s) 398, 488, 885, 1188
Bsc4I CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1174, 1326
Bse118I RCCGGY 3 cut(s) 366, 488, 1188
Bse1I ACTGG 1 cut(s) 1154
Bse3DI GCAATG 2 cut(s) 590, 828
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 3 cut(s) 302, 605, 1231
BseDI CCNNGG 2 cut(s) 604, 1134
BseGI GGATG 3 cut(s) 429, 679, 1055
BseLI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1174, 1326
BseMI GCAATG 2 cut(s) 590, 828
BseMII CTCAG 2 cut(s) 1239, 1449
BseNI ACTGG 1 cut(s) 1154
BseRI GAGGAG 1 cut(s) 492
BseXI GCAGC 5 cut(s) 215, 256, 1084, 1168, 1190
BshFI GGCC 2 cut(s) 241, 1229
BshNI GGYRCC 1 cut(s) 308
BshTI ACCGGT 2 cut(s) 488, 1188
BsiHKAI GWGCWC 3 cut(s) 190, 778, 1440
BsiSI CCGG 5 cut(s) 367, 399, 489, 886, 1189
BslFI GGGAC 1 cut(s) 1136
BslI CCNNNNNNNGG 5 cut(s) 458, 1135, 1160, 1174, 1326
BsmAI GTCTC 2 cut(s) 184, 426
BsmFI GGGAC 1 cut(s) 1136
BsnI GGCC 2 cut(s) 241, 1229
Bso31I GGTCTC 2 cut(s) 184, 426
Bsp1286I GDGCHC 3 cut(s) 190, 778, 1440
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 2 cut(s) 580, 1241
Bsp143I GATC 2 cut(s) 616, 811
BspACI CCGC 6 cut(s) 46, 236, 620, 902, 1034, 1390
BspANI GGCC 2 cut(s) 241, 1229
BspCNI CTCAG 2 cut(s) 1240, 1450
BspEI TCCGGA 1 cut(s) 398
BspHI TCATGA 2 cut(s) 562, 859
BspLI GGNNCC 4 cut(s) 240, 310, 487, 1032
BspMAI CTGCAG 4 cut(s) 274, 807, 877, 1102
BspPI GGATC 2 cut(s) 611, 819
BspT107I GGYRCC 1 cut(s) 308
BspTI CTTAAG 1 cut(s) 313
BspTNI GGTCTC 2 cut(s) 184, 426
BsrDI GCAATG 2 cut(s) 590, 828
BsrFI RCCGGY 3 cut(s) 366, 488, 1188
BsrGI TGTACA 2 cut(s) 580, 1241
BsrI ACTGG 1 cut(s) 1154
BssAI RCCGGY 3 cut(s) 366, 488, 1188
BssECI CCNNGG 2 cut(s) 604, 1134
BssMI GATC 2 cut(s) 616, 811
BssNI GRCGYC 1 cut(s) 1149
BssT1I CCWWGG 1 cut(s) 1134
Bst2UI CCWGG 3 cut(s) 302, 605, 1231
Bst4CI ACNGT 3 cut(s) 39, 290, 746
Bst6I CTCTTC 2 cut(s) 740, 1327
BstACI GRCGYC 1 cut(s) 1149
BstAFI CTTAAG 1 cut(s) 313
BstAUI TGTACA 2 cut(s) 580, 1241
BstC8I GCNNGC 2 cut(s) 115, 364
BstDEI CTNAG 2 cut(s) 1248, 1458
BstENI CCTNNNNNAGG 2 cut(s) 456, 1324
BstF5I GGATG 3 cut(s) 429, 679, 1055
BstKTI GATC 2 cut(s) 619, 814
BstMAI GTCTC 2 cut(s) 184, 426
BstMBI GATC 2 cut(s) 616, 811
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 3 cut(s) 302, 605, 1231
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 300, 603, 1229
BstSFI CTRYAG 4 cut(s) 270, 803, 873, 1098
BstV1I GCAGC 5 cut(s) 215, 256, 1084, 1168, 1190
BstX2I RGATCY 2 cut(s) 616, 811
BstXI CCANNNNNNTGG 2 cut(s) 175, 505
BstYI RGATCY 2 cut(s) 616, 811
BsuRI GGCC 2 cut(s) 241, 1229
BtsCI GGATG 3 cut(s) 429, 679, 1055
Cac8I GCNNGC 2 cut(s) 115, 364
CciI TCATGA 2 cut(s) 562, 859
Cfr10I RCCGGY 3 cut(s) 366, 488, 1188
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 1138
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 3 cut(s) 581, 1216, 1242
CspAI ACCGGT 2 cut(s) 488, 1188
CviAII CATG 6 cut(s) 57, 499, 563, 682, 860, 1442
CviQI GTAC 3 cut(s) 581, 1216, 1242
DdeI CTNAG 2 cut(s) 1248, 1458
DpnI GATC 2 cut(s) 618, 813
DpnII GATC 2 cut(s) 616, 811
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
Eam1104I CTCTTC 2 cut(s) 740, 1327
EarI CTCTTC 2 cut(s) 740, 1327
EciI GGCGGA 1 cut(s) 917
Ecl136II GAGCTC 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 1134
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 184, 426
Eco32I GATATC 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 188
EcoNI CCTNNNNNAGG 2 cut(s) 456, 1324
EcoRI GAATTC 2 cut(s) 677, 1465
EcoRII CCWGG 3 cut(s) 300, 603, 1229
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 6 cut(s) 60, 502, 566, 685, 863, 1445
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 1 cut(s) 1136
FatI CATG 6 cut(s) 56, 498, 562, 681, 859, 1441
FauI CCCGC 1 cut(s) 229
FblI GTMKAC 2 cut(s) 34, 879
Fnu4HI GCNGC 6 cut(s) 47, 204, 270, 1098, 1179, 1182
FokI GGATG 3 cut(s) 416, 686, 1062
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 6 cut(s) 47, 204, 270, 1098, 1179, 1182
FspBI CTAG 3 cut(s) 1037, 1202, 1314
GluI GCNGC 6 cut(s) 47, 204, 270, 1098, 1179, 1182
GsuI CTGGAG 1 cut(s) 762
HaeIII GGCC 2 cut(s) 241, 1229
HapII CCGG 5 cut(s) 367, 399, 489, 886, 1189
HgaI GACGC 1 cut(s) 1138
Hin1I GRCGYC 1 cut(s) 1149
Hin1II CATG 6 cut(s) 60, 502, 566, 685, 863, 1445
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 3 cut(s) 553, 1164, 1483
HpaII CCGG 5 cut(s) 367, 399, 489, 886, 1189
HphI GGTGA 4 cut(s) 17, 302, 441, 697
Hpy166II GTNNAC 5 cut(s) 35, 286, 800, 880, 1242
Hpy188I TCNGA 2 cut(s) 649, 1163
Hpy188III TCNNGA 9 cut(s) 183, 399, 563, 671, 779, 860, 939, 947, 962
Hpy8I GTNNAC 5 cut(s) 35, 286, 800, 880, 1242
HpyAV CCTTC 5 cut(s) 396, 788, 835, 1011, 1469
HpyCH4III ACNGT 3 cut(s) 39, 290, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 1248, 1458
Hsp92I GRCGYC 1 cut(s) 1149
Hsp92II CATG 6 cut(s) 60, 502, 566, 685, 863, 1445
Kpn2I TCCGGA 1 cut(s) 398
Kzo9I GATC 2 cut(s) 616, 811
LmnI GCTCC 4 cut(s) 781, 904, 1094, 1435
Lsp1109I GCAGC 5 cut(s) 215, 256, 1084, 1168, 1190
LweI GCATC 6 cut(s) 58, 190, 283, 349, 691, 1285
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 3 cut(s) 1037, 1202, 1314
MaeIII GTNAC 6 cut(s) 79, 211, 685, 1079, 1195, 1282
MalI GATC 2 cut(s) 618, 813
MboI GATC 2 cut(s) 616, 811
MboII GAAGA 6 cut(s) 273, 432, 626, 727, 1314, 1474
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 2 cut(s) 616, 811
MhlI GDGCHC 3 cut(s) 190, 778, 1440
MlyI GAGTC 1 cut(s) 1158
MmeI TCCRAC 2 cut(s) 351, 518
Mph1103I ATGCAT 1 cut(s) 706
MroI TCCGGA 1 cut(s) 398
MroXI GAANNNNTTC 3 cut(s) 345, 987, 1321
MseI TTAA 5 cut(s) 314, 707, 713, 1025, 1406
MslI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
MspA1I CMGCKG 2 cut(s) 1181, 1247
MspCI CTTAAG 1 cut(s) 313
MspI CCGG 5 cut(s) 367, 399, 489, 886, 1189
MspR9I CCNGG 3 cut(s) 302, 605, 1231
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 3 cut(s) 302, 605, 1231
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 2 cut(s) 616, 811
NlaIII CATG 6 cut(s) 60, 502, 566, 685, 863, 1445
NlaIV GGNNCC 4 cut(s) 240, 310, 487, 1032
NmuCI GTSAC 5 cut(s) 79, 685, 1079, 1195, 1282
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 606
PagI TCATGA 2 cut(s) 562, 859
PdmI GAANNNNTTC 3 cut(s) 345, 987, 1321
PfeI GAWTC 2 cut(s) 553, 1483
PflMI CCANNNNNTGG 1 cut(s) 1135
PinAI ACCGGT 2 cut(s) 488, 1188
PkrI GCNGC 6 cut(s) 48, 205, 271, 1099, 1180, 1183
PleI GAGTC 1 cut(s) 1158
PpsI GAGTC 1 cut(s) 1158
PshBI ATTAAT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 300, 603, 1229
PspGI CCWGG 3 cut(s) 300, 603, 1229
PspN4I GGNNCC 4 cut(s) 240, 310, 487, 1032
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 4 cut(s) 274, 807, 877, 1102
PsuI RGATCY 2 cut(s) 616, 811
PvuII CAGCTG 2 cut(s) 1181, 1247
RsaI GTAC 3 cut(s) 582, 1217, 1243
RsaNI GTAC 3 cut(s) 581, 1216, 1242
RseI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
SacI GAGCTC 1 cut(s) 190
SaqAI TTAA 5 cut(s) 314, 707, 713, 1025, 1406
SatI GCNGC 6 cut(s) 47, 204, 270, 1098, 1179, 1182
Sau3AI GATC 2 cut(s) 616, 811
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 1158
ScrFI CCNGG 3 cut(s) 302, 605, 1231
SduI GDGCHC 3 cut(s) 190, 778, 1440
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 6 cut(s) 58, 190, 283, 349, 691, 1285
SfcI CTRYAG 4 cut(s) 270, 803, 873, 1098
SmiMI CAYNNNNRTG 4 cut(s) 173, 503, 561, 606
SmlI CTYRAG 5 cut(s) 67, 181, 207, 313, 947
SmoI CTYRAG 5 cut(s) 67, 181, 207, 313, 947
SsiI CCGC 6 cut(s) 46, 236, 620, 902, 1034, 1390
SspI AATATT 2 cut(s) 711, 1430
SspMI CTAG 3 cut(s) 1037, 1202, 1314
SstI GAGCTC 1 cut(s) 190
StyD4I CCNGG 3 cut(s) 300, 603, 1229
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 3 cut(s) 39, 290, 746
TaqI TCGA 1 cut(s) 738
TatI WGTACW 2 cut(s) 580, 1241
TauI GCSGC 1 cut(s) 49
TfiI GAWTC 2 cut(s) 553, 1483
Tru1I TTAA 5 cut(s) 314, 707, 713, 1025, 1406
Tru9I TTAA 5 cut(s) 314, 707, 713, 1025, 1406
TseFI GTSAC 5 cut(s) 79, 685, 1079, 1195, 1282
TseI GCWGC 5 cut(s) 203, 269, 1097, 1178, 1181
Tsp45I GTSAC 5 cut(s) 79, 685, 1079, 1195, 1282
TspGWI ACGGA 1 cut(s) 1062
Van91I CCANNNNNTGG 1 cut(s) 1135
Vha464I CTTAAG 1 cut(s) 313
VspI ATTAAT 1 cut(s) 707
XagI CCTNNNNNAGG 2 cut(s) 456, 1324
XapI RAATTY 3 cut(s) 677, 956, 1465
XceI RCATGY 1 cut(s) 60
XmiI GTMKAC 2 cut(s) 34, 879
XmnI GAANNNNTTC 3 cut(s) 345, 987, 1321
XspI CTAG 3 cut(s) 1037, 1202, 1314
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.