AT4G33580

Reversible hydration of carbon dioxide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001103
Physical Location & Seq
Forward (+)
16138251 .. 16141624
3374 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33580.2

Sequence Viewer

Length: 906 bp
ATGGCAGCCACTCCCACACACTTCTCTGTCTCCCATGATCCTTTTTCTTCCACGTCTCTCCTTAATCTCCAAACTCAAGCGATCTTTGGTCCCAATCACAGTTTAAAGACAACCCAGTTGAGAATTCCAGCTTCTTTCAGAAGAAAAGCTACAAACTTGCAAGTGATGGCTTCAGGAAAGACACCTGGACTGACTCAGGAAGCTAATGGGGTTGCAATTGATAGACAAAACAACACTGATGTATTTGACGACATGAAACAGCGGTTCCTGGCCTTCAAGAAGCTTAAGTACATGGATGACTTTGAACACTACAAAAATCTGGCAGATGCTCAAGCTCCAAAGTTTCTGGTGATTGCTTGTGCAGACTCTAGAGTTTGTCCTTCTGCTGTCCTGGGATTCCAACCGGGTGACGCATTCACTGTTCGTAACATTGCAAATTTAGTACCTCCATATGAGTCTGGACCTACTGAAACCAAAGCTGCTCTAGAGTTCTCTGTGAATACTCTTAATGTGGAAAACATCTTAGTCATTGGTCATAGCCGGTGTGGAGGAATTCAAGCTTTAATGAAAATGGAAGACGAAGGAGATTCCAGAAGTTTCATACACAACTGGGTAGTTGTGGGAAAGAAGGCAAAGGAAAGCACAAAAGCTGTTGCTTCAAACCTCCATTTTGATCATCAGTGCCAACATTGTGAAAAGGCATCGATAAATCATTCATTAGAAAGGCTGCTTGGGTACCCGTGGATAGAAGAGAAAGTGCGGCAAGGTTCACTGTCTCTCCATGGTGGATACTATAATTTTGTTGATTGTACGTTCGAGAAATGGACAGTGGATTATGCAGCAAGCAGAGGTAAGAAGAAGGAAGGCAGTGGAATCGCTGTTAAAGACCGGTCAGTTTGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Pro_CA

Protein Analysis

301

Amino Acids

33.4

Weight (kDa)

8.64

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_CA PF00484 117 - 270 8.4e-42 Carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 735
AccB1I GGYRCC 1 cut(s) 735
AciI CCGC 2 cut(s) 262, 760
AclWI GGATC 1 cut(s) 32
AcsI RAATTY 3 cut(s) 123, 436, 552
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 4 cut(s) 290, 444, 737, 811
AflII CTTAAG 1 cut(s) 284
AgeI ACCGGT 1 cut(s) 888
AgsI TTSAA 4 cut(s) 277, 305, 557, 660
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 3 cut(s) 184, 267, 390
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
AluBI AGCT 8 cut(s) 131, 149, 203, 283, 335, 479, 560, 650
AluI AGCT 8 cut(s) 131, 149, 203, 283, 335, 479, 560, 650
Alw26I GTCTC 3 cut(s) 34, 60, 780
AlwI GGATC 1 cut(s) 32
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 4 cut(s) 5, 479, 727, 839
ApoI RAATTY 3 cut(s) 123, 436, 552
AsiGI ACCGGT 1 cut(s) 888
Asp718I GGTACC 1 cut(s) 735
AspS9I GGNCC 2 cut(s) 89, 461
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 2 cut(s) 361, 419
AvaII GGWCC 2 cut(s) 89, 461
BanI GGYRCC 1 cut(s) 735
BarI GAAGNNNNNNTAC 4 cut(s) 133, 165, 272, 304
BbsI GAAGAC 1 cut(s) 582
BbvI GCAGC 4 cut(s) 17, 466, 714, 851
BccI CCATC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciT130I CCWGG 3 cut(s) 186, 269, 392
BciVI GTATCC 1 cut(s) 782
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 3 cut(s) 34, 60, 780
BfaI CTAG 2 cut(s) 369, 485
BfrI CTTAAG 1 cut(s) 284
BfuI GTATCC 1 cut(s) 782
BisI GCNGC 5 cut(s) 6, 480, 728, 761, 840
BlsI GCNGC 5 cut(s) 7, 481, 729, 762, 841
Bme1390I CCNGG 4 cut(s) 186, 269, 392, 405
Bme18I GGWCC 2 cut(s) 89, 461
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 2 cut(s) 89, 461
BmiI GGNNCC 3 cut(s) 91, 266, 737
BmrFI CCNGG 4 cut(s) 186, 269, 392, 405
BmrI ACTGGG 2 cut(s) 109, 619
BmsI GCATC 2 cut(s) 316, 710
BmuI ACTGGG 2 cut(s) 109, 619
BpiI GAAGAC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 60, 315
BpuMI CCSGG 1 cut(s) 405
Bsa29I ATCGAT 1 cut(s) 704
BsaJI CCNNGG 3 cut(s) 391, 740, 781
BsaWI WCCGGW 1 cut(s) 888
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bse118I RCCGGY 2 cut(s) 540, 888
Bse1I ACTGG 2 cut(s) 115, 614
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 3 cut(s) 186, 269, 392
BseCI ATCGAT 1 cut(s) 704
BseDI CCNNGG 3 cut(s) 391, 740, 781
BseGI GGATG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 2 cut(s) 115, 614
BseXI GCAGC 4 cut(s) 17, 466, 714, 851
BsgI GTGCAG 1 cut(s) 381
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 1 cut(s) 735
BshTI ACCGGT 1 cut(s) 888
BshVI ATCGAT 1 cut(s) 704
BsiSI CCGG 3 cut(s) 404, 541, 889
BslFI GGGAC 1 cut(s) 75
BsmAI GTCTC 3 cut(s) 34, 60, 780
BsmBI CGTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 37, 81, 673
Bsp19I CCATGG 1 cut(s) 781
BspACI CCGC 2 cut(s) 262, 760
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 1 cut(s) 208
BspDI ATCGAT 1 cut(s) 704
BspLI GGNNCC 3 cut(s) 91, 266, 737
BspPI GGATC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 735
BspTI CTTAAG 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 2 cut(s) 540, 888
BsrI ACTGG 2 cut(s) 115, 614
BssAI RCCGGY 2 cut(s) 540, 888
BssECI CCNNGG 3 cut(s) 391, 740, 781
BssMI GATC 3 cut(s) 37, 81, 673
BssT1I CCWWGG 1 cut(s) 781
Bst2UI CCWGG 3 cut(s) 186, 269, 392
Bst4CI ACNGT 4 cut(s) 101, 421, 774, 829
Bst6I CTCTTC 1 cut(s) 744
BstAFI CTTAAG 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 844
BstDEI CTNAG 2 cut(s) 195, 523
BstDSI CCRYGG 2 cut(s) 740, 781
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 3 cut(s) 40, 84, 676
BstMAI GTCTC 3 cut(s) 34, 60, 780
BstMBI GATC 3 cut(s) 37, 81, 673
BstNI CCWGG 3 cut(s) 186, 269, 392
BstSCI CCNGG 4 cut(s) 184, 267, 390, 403
BstV1I GCAGC 4 cut(s) 17, 466, 714, 851
BstV2I GAAGAC 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 704
BsuI GTATCC 1 cut(s) 782
BsuRI GGCC 1 cut(s) 272
BsuTUI ATCGAT 1 cut(s) 704
BtgI CCRYGG 2 cut(s) 740, 781
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 1 cut(s) 874
BtsIMutI CAGTG 6 cut(s) 234, 417, 686, 770, 834, 874
Cac8I GCNNGC 1 cut(s) 844
Cfr10I RCCGGY 2 cut(s) 540, 888
Cfr13I GGNCC 2 cut(s) 89, 461
ClaI ATCGAT 1 cut(s) 704
CseI GACGC 1 cut(s) 419
Csp6I GTAC 4 cut(s) 289, 443, 736, 810
CspAI ACCGGT 1 cut(s) 888
CviAII CATG 4 cut(s) 35, 253, 292, 782
CviQI GTAC 4 cut(s) 289, 443, 736, 810
DdeI CTNAG 2 cut(s) 195, 523
DpnI GATC 3 cut(s) 39, 83, 675
DpnII GATC 3 cut(s) 37, 81, 673
DraI TTTAAA 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 781
Eco47I GGWCC 2 cut(s) 89, 461
Eco57I CTGAAG 1 cut(s) 156
EcoRI GAATTC 2 cut(s) 123, 552
EcoRII CCWGG 3 cut(s) 184, 267, 390
EcoT14I CCWWGG 1 cut(s) 781
ErhI CCWWGG 1 cut(s) 781
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 4 cut(s) 38, 256, 295, 785
FaqI GGGAC 1 cut(s) 75
FatI CATG 4 cut(s) 34, 252, 291, 781
FauNDI CATATG 1 cut(s) 451
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 5 cut(s) 6, 480, 728, 761, 840
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 5 cut(s) 6, 480, 728, 761, 840
FspBI CTAG 2 cut(s) 369, 485
GluI GCNGC 5 cut(s) 6, 480, 728, 761, 840
HaeIII GGCC 1 cut(s) 272
HapII CCGG 3 cut(s) 404, 541, 889
HgaI GACGC 1 cut(s) 419
Hin1II CATG 4 cut(s) 38, 256, 295, 785
HindIII AAGCTT 2 cut(s) 281, 558
HinfI GANTC 6 cut(s) 193, 365, 396, 455, 587, 873
HpaII CCGG 3 cut(s) 404, 541, 889
HphI GGTGA 2 cut(s) 361, 419
Hpy166II GTNNAC 1 cut(s) 770
Hpy188I TCNGA 1 cut(s) 140
Hpy188III TCNNGA 9 cut(s) 174, 197, 277, 369, 459, 485, 591, 817, 903
Hpy8I GTNNAC 1 cut(s) 770
HpyAV CCTTC 6 cut(s) 283, 390, 575, 622, 853, 857
HpyCH4III ACNGT 4 cut(s) 101, 421, 774, 829
HpyCH4IV ACGT 2 cut(s) 53, 812
HpyCH4V TGCA 5 cut(s) 160, 215, 362, 434, 839
HpyF3I CTNAG 2 cut(s) 195, 523
HpySE526I ACGT 2 cut(s) 53, 812
Hsp92II CATG 4 cut(s) 38, 256, 295, 785
KpnI GGTACC 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 3 cut(s) 37, 81, 673
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 4 cut(s) 17, 466, 714, 851
LweI GCATC 2 cut(s) 316, 710
MaeI CTAG 2 cut(s) 369, 485
MaeII ACGT 2 cut(s) 53, 812
MaeIII GTNAC 2 cut(s) 407, 425
MalI GATC 3 cut(s) 39, 83, 675
MboI GATC 3 cut(s) 37, 81, 673
MboII GAAGA 5 cut(s) 39, 153, 587, 761, 868
MfeI CAATTG 1 cut(s) 216
MluCI AATT 5 cut(s) 123, 216, 436, 552, 796
MlyI GAGTC 3 cut(s) 187, 359, 464
MmeI TCCRAC 1 cut(s) 424
MnlI CCTC 4 cut(s) 456, 542, 674, 842
MseI TTAA 6 cut(s) 63, 104, 285, 507, 563, 882
MspA1I CMGCKG 1 cut(s) 262
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 3 cut(s) 404, 541, 889
MspR9I CCNGG 4 cut(s) 186, 269, 392, 405
MunI CAATTG 1 cut(s) 216
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 3 cut(s) 186, 269, 392
NciI CCSGG 1 cut(s) 405
NcoI CCATGG 1 cut(s) 781
NdeI CATATG 1 cut(s) 451
NdeII GATC 3 cut(s) 37, 81, 673
NlaIII CATG 4 cut(s) 38, 256, 295, 785
NlaIV GGNNCC 3 cut(s) 91, 266, 737
NmuCI GTSAC 1 cut(s) 407
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 396, 587, 873
PinAI ACCGGT 1 cut(s) 888
PkrI GCNGC 5 cut(s) 7, 481, 729, 762, 841
PleI GAGTC 3 cut(s) 187, 359, 463
PpsI GAGTC 3 cut(s) 187, 359, 463
Psp6I CCWGG 3 cut(s) 184, 267, 390
PspGI CCWGG 3 cut(s) 184, 267, 390
PspN4I GGNNCC 3 cut(s) 91, 266, 737
PspPI GGNCC 2 cut(s) 89, 461
RsaI GTAC 4 cut(s) 290, 444, 737, 811
RsaNI GTAC 4 cut(s) 289, 443, 736, 810
SaqAI TTAA 6 cut(s) 63, 104, 285, 507, 563, 882
SatI GCNGC 5 cut(s) 6, 480, 728, 761, 840
Sau3AI GATC 3 cut(s) 37, 81, 673
Sau96I GGNCC 2 cut(s) 89, 461
SchI GAGTC 3 cut(s) 187, 359, 464
ScrFI CCNGG 4 cut(s) 186, 269, 392, 405
SfaNI GCATC 2 cut(s) 316, 710
SinI GGWCC 2 cut(s) 89, 461
SmlI CTYRAG 3 cut(s) 75, 284, 330
SmoI CTYRAG 3 cut(s) 75, 284, 330
Sse9I AATT 5 cut(s) 123, 216, 436, 552, 796
SsiI CCGC 2 cut(s) 262, 760
SspMI CTAG 2 cut(s) 369, 485
StyD4I CCNGG 4 cut(s) 184, 267, 390, 403
StyI CCWWGG 1 cut(s) 781
TaaI ACNGT 4 cut(s) 101, 421, 774, 829
TaiI ACGT 2 cut(s) 56, 815
TaqI TCGA 2 cut(s) 704, 816
TasI AATT 5 cut(s) 123, 216, 436, 552, 796
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 3 cut(s) 396, 587, 873
Tru1I TTAA 6 cut(s) 63, 104, 285, 507, 563, 882
Tru9I TTAA 6 cut(s) 63, 104, 285, 507, 563, 882
TscAI CASTG 6 cut(s) 241, 424, 686, 777, 834, 874
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 4 cut(s) 5, 479, 727, 839
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 4 cut(s) 269, 581, 589, 705
TspRI CASTG 6 cut(s) 241, 424, 686, 777, 834, 874
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 89, 461
XapI RAATTY 3 cut(s) 123, 436, 552
XbaI TCTAGA 2 cut(s) 368, 484
XspI CTAG 2 cut(s) 369, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.