AT4G33920

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001678
Physical Location & Seq
Forward (+)
16260481 .. 16263054
2574 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33920.2

Sequence Viewer

Length: 1143 bp
ATGTTGCGAGCTTTAGCACGGCCTCTCGAACGGTGTTTGGGAAGCAGAGCTAGTGGTGATGGTTTACTCTGGCAATCGGAATTGAGACCTCACGCTGGCGGTGATTATTCGATCGCGGTGGTTCAAGCCAATTCCAGGCTTGAAGATCAGAGTCAGGTTTTCACATCTTCTTCTGCTACTTACGTCGGTGTATACGATGGTCATGGTGGACCTGAAGCTTCTAGATTCGTTAACAGACATCTCTTTCCTTATATGCACAAATTTGCAAGAGAACATGGGGGATTATCTGTAGATGTTATCAAAAAGGCATTCAAAGAAACAGAAGAAGAGTTTTGTGGTATGGTTAAACGATCCCTTCCCATGAAACCGCAAATGGCTACTGTAGGATCTTGCTGTCTTGTTGGTGCAATCTCTAATGACACACTGTATGTTGCTAATCTTGGGGACTCGAGAGCCGTTCTTGGAAGCGTTGTTTCAGGGGTTGATAGTAATAAAGGTGCCGTAGCTGAACGGTTATCTACTGATCATAATGTTGCTGTTGAAGAAGTGAGAAAGGAGGTTAAGGCACTTAACCCTGATGACTCACAAATCGTCTTATACACACGTGGAGTTTGGCGGATTAAAGGCATTATTCAGGTATCGAGATCAATTGGGGATGTATACTTGAAAAAACCGGAGTATTACAGGGACCCGATTTTCCAGCGACATGGAAATCCCATTCCTTTGAGGAGACCCGCGATGACAGCCGAACCCTCCATTATAGTAAGGAAGCTTAAGCCACAGGACTTGTTTCTGATATTTGCATCAGATGGTCTCTGGGAACATCTTAGTGATGAAACAGCCGTAGAAATCGTCCTCAAACACCCAAGAACTGGTATTGCCCGAAGACTTGTAAGAGCTGCTCTGGAAGAAGCGGCAAAGAAGAGAGAAATGAGATATGGAGATATAAAGAAAATAGCCAAAGGAATTCGACGACATTTCCATGACGACATAAGCGTTATTGTAGTTTATCTAGATCAAAACAAAACCAGTTCATCGAATAGTAAATTGGTGAAGCAAGGAGGTATCACCGCTCCACCGGATATCTACTCATTACACTCTGATGAAGCAGAGCAACGACGGTTACTCAATGTGTTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

380

Amino Acids

42.32

Weight (kDa)

9.25

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 14 - 224 2.5e-33 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 497
AccB7I CCANNNNNTGG 1 cut(s) 872
AccBSI CCGCTC 1 cut(s) 1073
AccI GTMKAC 2 cut(s) 192, 660
AccII CGCG 2 cut(s) 116, 737
AciI CCGC 7 cut(s) 99, 116, 368, 616, 735, 914, 1071
AclWI GGATC 2 cut(s) 345, 394
AcsI RAATTY 2 cut(s) 260, 966
AcuI CTGAAG 1 cut(s) 234
AcvI CACGTG 1 cut(s) 605
AfiI CCNNNNNNNGG 3 cut(s) 95, 135, 872
AflII CTTAAG 1 cut(s) 773
AflIII ACRYGT 1 cut(s) 602
AgsI TTSAA 5 cut(s) 125, 143, 313, 542, 667
AjnI CCWGG 1 cut(s) 134
AjuI GAANNNNNNNTTGG 2 cut(s) 1031, 1063
AluBI AGCT 6 cut(s) 11, 50, 218, 506, 772, 899
AluI AGCT 6 cut(s) 11, 50, 218, 506, 772, 899
Alw26I GTCTC 3 cut(s) 79, 724, 818
AlwI GGATC 2 cut(s) 345, 394
Ama87I CYCGRG 1 cut(s) 448
AoxI GGCC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 2 cut(s) 260, 966
AspS9I GGNCC 2 cut(s) 209, 688
AsuHPI GGTGA 4 cut(s) 68, 113, 1060, 1063
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 2 cut(s) 209, 688
BanI GGYRCC 1 cut(s) 497
BbrPI CACGTG 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 892
BbvI GCAGC 1 cut(s) 886
BccI CCATC 3 cut(s) 53, 191, 803
BceAI ACGGC 4 cut(s) 35, 440, 485, 827
BciT130I CCWGG 1 cut(s) 136
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 3 cut(s) 79, 724, 818
BfaI CTAG 3 cut(s) 51, 222, 1013
BfmI CTRYAG 2 cut(s) 288, 381
BfrI CTTAAG 1 cut(s) 773
BisI GCNGC 2 cut(s) 900, 915
BlsI GCNGC 2 cut(s) 901, 916
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 2 cut(s) 209, 688
BmeT110I CYCGRG 1 cut(s) 448
BmgT120I GGNCC 2 cut(s) 209, 688
BmiI GGNNCC 3 cut(s) 499, 689, 690
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 812
BpiI GAAGAC 1 cut(s) 892
BsaAI YACGTR 1 cut(s) 605
BsaI GGTCTC 3 cut(s) 79, 724, 818
BsaWI WCCGGW 2 cut(s) 673, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 135, 872
Bse1I ACTGG 2 cut(s) 877, 1029
BseBI CCWGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 3 cut(s) 95, 135, 872
BseNI ACTGG 2 cut(s) 877, 1029
BseRI GAGGAG 1 cut(s) 742
BseXI GCAGC 1 cut(s) 886
Bsh1236I CGCG 2 cut(s) 116, 737
Bsh1285I CGRYCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 22
BshNI GGYRCC 1 cut(s) 497
BsiEI CGRYCG 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 448
BsiSI CCGG 2 cut(s) 674, 1079
BslFI GGGAC 2 cut(s) 458, 701
BslI CCNNNNNNNGG 3 cut(s) 95, 135, 872
BsmAI GTCTC 3 cut(s) 79, 724, 818
BsmFI GGGAC 2 cut(s) 458, 701
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 22
Bso31I GGTCTC 3 cut(s) 79, 724, 818
BsoBI CYCGRG 1 cut(s) 448
Bsp143I GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
BspACI CCGC 7 cut(s) 99, 116, 368, 616, 735, 914, 1071
BspANI GGCC 1 cut(s) 22
BspFNI CGCG 2 cut(s) 116, 737
BspLI GGNNCC 3 cut(s) 499, 689, 690
BspPI GGATC 2 cut(s) 345, 394
BspT107I GGYRCC 1 cut(s) 497
BspTI CTTAAG 1 cut(s) 773
BspTNI GGTCTC 3 cut(s) 79, 724, 818
BsrBI CCGCTC 1 cut(s) 1073
BsrI ACTGG 2 cut(s) 877, 1029
BssMI GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
BssNAI GTATAC 2 cut(s) 193, 661
Bst1107I GTATAC 2 cut(s) 193, 661
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 5 cut(s) 33, 382, 426, 513, 1122
Bst6I CTCTTC 2 cut(s) 321, 917
BstAFI CTTAAG 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 605
BstC8I GCNNGC 2 cut(s) 9, 97
BstDEI CTNAG 1 cut(s) 827
BstF5I GGATG 1 cut(s) 661
BstFNI CGCG 2 cut(s) 116, 737
BstKTI GATC 7 cut(s) 114, 148, 353, 389, 526, 647, 1018
BstMAI GTCTC 3 cut(s) 79, 724, 818
BstMBI GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
BstMCI CGRYCG 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 2 cut(s) 288, 381
BstUI CGCG 2 cut(s) 116, 737
BstV1I GCAGC 1 cut(s) 886
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 1 cut(s) 386
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 1 cut(s) 386
BstZ17I GTATAC 2 cut(s) 193, 661
BsuRI GGCC 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 752
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 9, 97
Cfr13I GGNCC 2 cut(s) 209, 688
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviAII CATG 5 cut(s) 203, 275, 361, 707, 983
DdeI CTNAG 1 cut(s) 827
DpnI GATC 7 cut(s) 113, 147, 352, 388, 525, 646, 1017
DpnII GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
Eam1104I CTCTTC 2 cut(s) 321, 917
EarI CTCTTC 2 cut(s) 321, 917
EciI GGCGGA 1 cut(s) 631
Eco31I GGTCTC 3 cut(s) 79, 724, 818
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 2 cut(s) 209, 688
Eco57I CTGAAG 1 cut(s) 234
Eco72I CACGTG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 688
EcoRI GAATTC 1 cut(s) 966
EcoRII CCWGG 1 cut(s) 134
EcoRV GATATC 1 cut(s) 1084
FaeI CATG 5 cut(s) 206, 278, 364, 710, 986
FaqI GGGAC 2 cut(s) 458, 701
FatI CATG 5 cut(s) 202, 274, 360, 706, 982
FauI CCCGC 1 cut(s) 742
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 2 cut(s) 192, 660
Fnu4HI GCNGC 2 cut(s) 900, 915
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 2 cut(s) 900, 915
FspBI CTAG 3 cut(s) 51, 222, 1013
GluI GCNGC 2 cut(s) 900, 915
HaeIII GGCC 1 cut(s) 22
HapII CCGG 2 cut(s) 674, 1079
Hin1II CATG 5 cut(s) 206, 278, 364, 710, 986
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 2 cut(s) 216, 770
HinfI GANTC 4 cut(s) 151, 225, 446, 581
HpaI GTTAAC 1 cut(s) 232
HpaII CCGG 2 cut(s) 674, 1079
HphI GGTGA 4 cut(s) 68, 113, 1060, 1063
Hpy166II GTNNAC 5 cut(s) 65, 193, 209, 232, 661
Hpy188I TCNGA 5 cut(s) 79, 150, 795, 808, 1102
Hpy188III TCNNGA 6 cut(s) 26, 222, 450, 642, 905, 1013
Hpy8I GTNNAC 5 cut(s) 65, 193, 209, 232, 661
Hpy99I CGWCG 3 cut(s) 188, 975, 1122
HpyAV CCTTC 1 cut(s) 365
HpyCH4III ACNGT 5 cut(s) 33, 382, 426, 513, 1122
HpyCH4IV ACGT 2 cut(s) 183, 604
HpyCH4V TGCA 4 cut(s) 256, 266, 407, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 1 cut(s) 827
HpySE526I ACGT 2 cut(s) 183, 604
Hsp92II CATG 5 cut(s) 206, 278, 364, 710, 986
KflI GGGWCCC 1 cut(s) 688
Ksp22I TGATCA 1 cut(s) 523
KspAI GTTAAC 1 cut(s) 232
Kzo9I GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
LmnI GCTCC 1 cut(s) 1078
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 1 cut(s) 812
MaeI CTAG 3 cut(s) 51, 222, 1013
MaeII ACGT 2 cut(s) 183, 604
MaeIII GTNAC 1 cut(s) 1122
MalI GATC 7 cut(s) 113, 147, 352, 388, 525, 646, 1017
MbiI CCGCTC 1 cut(s) 1073
MboI GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
MboII GAAGA 9 cut(s) 155, 159, 162, 335, 338, 554, 897, 920, 934
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 386
MluCI AATT 6 cut(s) 80, 130, 260, 648, 966, 1046
MlyI GAGTC 3 cut(s) 160, 440, 575
MnlI CCTC 7 cut(s) 33, 99, 550, 720, 763, 866, 1055
MseI TTAA 6 cut(s) 231, 345, 561, 570, 621, 774
MslI CAYNNNNRTG 3 cut(s) 828, 981, 1101
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 2 cut(s) 674, 1079
MspR9I CCNGG 1 cut(s) 136
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 1 cut(s) 136
MvnI CGCG 2 cut(s) 116, 737
MwoI GCNNNNNNNGC 1 cut(s) 743
NdeII GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
NlaIII CATG 5 cut(s) 206, 278, 364, 710, 986
NlaIV GGNNCC 3 cut(s) 499, 689, 690
PaeR7I CTCGAG 1 cut(s) 448
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 993
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 1 cut(s) 225
PflMI CCANNNNNTGG 1 cut(s) 872
PkrI GCNGC 2 cut(s) 901, 916
Ple19I CGATCG 1 cut(s) 114
PleI GAGTC 3 cut(s) 159, 440, 575
PmaCI CACGTG 1 cut(s) 605
PmlI CACGTG 1 cut(s) 605
PpsI GAGTC 3 cut(s) 159, 440, 575
Ppu21I YACGTR 1 cut(s) 605
PpuMI RGGWCCY 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 1 cut(s) 134
PspCI CACGTG 1 cut(s) 605
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 3 cut(s) 499, 689, 690
PspPI GGNCC 2 cut(s) 209, 688
PspPPI RGGWCCY 1 cut(s) 688
PsuI RGATCY 1 cut(s) 386
PvuI CGATCG 1 cut(s) 114
RseI CAYNNNNRTG 3 cut(s) 828, 981, 1101
SaqAI TTAA 6 cut(s) 231, 345, 561, 570, 621, 774
SatI GCNGC 2 cut(s) 900, 915
Sau3AI GATC 7 cut(s) 111, 145, 350, 386, 523, 644, 1015
Sau96I GGNCC 2 cut(s) 209, 688
SchI GAGTC 3 cut(s) 160, 440, 575
ScrFI CCNGG 1 cut(s) 136
SfaNI GCATC 1 cut(s) 812
SfcI CTRYAG 2 cut(s) 288, 381
Sfr274I CTCGAG 1 cut(s) 448
SinI GGWCC 2 cut(s) 209, 688
SlaI CTCGAG 1 cut(s) 448
SmiMI CAYNNNNRTG 3 cut(s) 828, 981, 1101
SmlI CTYRAG 2 cut(s) 448, 773
SmoI CTYRAG 2 cut(s) 448, 773
Sse9I AATT 6 cut(s) 80, 130, 260, 648, 966, 1046
SsiI CCGC 7 cut(s) 99, 116, 368, 616, 735, 914, 1071
SspMI CTAG 3 cut(s) 51, 222, 1013
StyD4I CCNGG 1 cut(s) 134
TaaI ACNGT 5 cut(s) 33, 382, 426, 513, 1122
TaiI ACGT 2 cut(s) 186, 607
TaqI TCGA 6 cut(s) 27, 110, 449, 641, 970, 1037
TasI AATT 6 cut(s) 80, 130, 260, 648, 966, 1046
TauI GCSGC 1 cut(s) 917
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 6 cut(s) 231, 345, 561, 570, 621, 774
Tru9I TTAA 6 cut(s) 231, 345, 561, 570, 621, 774
TscAI CASTG 1 cut(s) 429
TseI GCWGC 1 cut(s) 899
TspDTI ATGAA 4 cut(s) 377, 849, 1023, 1119
TspRI CASTG 1 cut(s) 429
Van91I CCANNNNNTGG 1 cut(s) 872
Vha464I CTTAAG 1 cut(s) 773
VpaK11BI GGWCC 2 cut(s) 209, 688
XapI RAATTY 2 cut(s) 260, 966
XbaI TCTAGA 2 cut(s) 221, 1012
XhoI CTCGAG 1 cut(s) 448
XmiI GTMKAC 2 cut(s) 192, 660
XspI CTAG 3 cut(s) 51, 222, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.