pycom02g14740

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001678
Physical Location & Seq
Reverse (-)
11337437 .. 11340968
3532 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14740.4

Sequence Viewer

Length: 1137 bp
ATGATGTTGGAGATGTGTCGGAGGCCGTTGGAGAAGTGTTTTGGTGGCGGTGACGGCGGCGATGGGCTGCTTTGGCACATGGACCTCAAGCCCCACTCTTCTGGGGACTATTCGATTGCAGTGGTTCAAGCTAATTCTGCTCTGGAGGATCAGGGACAGGTGTTCACATCTCCTTGCGCCACGTATGTTGGAGTCTATGACGGCCATGGCGGTCCTGAAGCTTCCCGCTTCATCACCAACCGCCTCTTCCCCTTCCTTCACAAGTTTGCAACTGAACAAGGTGGACTGTCAGAGGATGTGATAAGGAAGGCATTTGATGCAACAGAGGAGGAGTTCTTGGAGTTGGTTAAGCGATCATGGATGGTGCGGCCGCAGATTGCTTCAGTAGGATCGTGTTGTCTGGTTGGTGCAATTGCAGATGGTATTTTATATGTGGCTAATCTCGGAGATTCGAGGGCAGTTCTTGGCAGGAGAGCATCGGATGGCCAGGCCGTGGTCGCAGAGCGGTTGTCTACGGATCACAATGTTTCAGTGGAGGAAGTGAGGAAGGAGGTTAAGGATCTTCACCCTGATGATGCGCACATTGTGGTGTATACACATGGAGTTTGGCGAATTAAGGGCATTATTCAGGTCTCAAGGTCAATCGGTGATGTCTACTTGAAGAAACCTGAGTTTAACAGATATCCTCTCTTCCACCAGTTCGGTATGCCCATTCCTTTAAAGAAGCCTGTAATGACAGCAGAACCGTCGATAGTAGTTCGAAAGTTACAACCACAGGACATGTTTTTGATATTTGCAACAGATGGTCTCTGGGAGCATTTAAGTGATGAAACAGCCGTGAAAATCGTCTCCAAAAATCCAAGAGCTGGAATAGCAAAGCGATTGGTAAGAGCTGCCATCGACGAGGCTGCAAGGAAAAGAGAATTGAGATATGACGACATAAAAAGAATTGAAAGGGGGGTAAGGCGCCATTTCCACGACGATATCACTGTAATCGTGATATTCCTAGATCATTCACAAGTTTCCCCAAATGCTGGTGTGAAGGACCAGAGCTTCTTTAACTGCACTAGTGTCCCTGTCGATATATTCTCTATGAACGAGGAAGCAGCAGACGGATCACTCCACACCTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

379

Amino Acids

42.13

Weight (kDa)

6.17

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 579
AccB1I GGYRCC 1 cut(s) 966
AccB7I CCANNNNNTGG 3 cut(s) 493, 866, 1034
AccBSI CCGCTC 1 cut(s) 505
AccI GTMKAC 3 cut(s) 512, 593, 654
AciI CCGC 8 cut(s) 48, 57, 210, 226, 241, 367, 371, 505
AclWI GGATC 5 cut(s) 156, 397, 525, 567, 1123
AcoI YGGCCR 3 cut(s) 202, 368, 484
AcuI CTGAAG 2 cut(s) 237, 366
AcyI GRCGYC 1 cut(s) 967
AdeI CACNNNGTG 1 cut(s) 586
AfiI CCNNNNNNNGG 3 cut(s) 493, 866, 1034
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 3 cut(s) 128, 661, 953
AhlI ACTAGT 1 cut(s) 1067
AjnI CCWGG 1 cut(s) 486
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 5 cut(s) 131, 221, 866, 893, 1053
AluI AGCT 5 cut(s) 131, 221, 866, 893, 1053
Alw26I GTCTC 3 cut(s) 637, 812, 853
AlwI GGATC 5 cut(s) 156, 397, 525, 567, 1123
AoxI GGCC 5 cut(s) 23, 202, 368, 484, 489
ApeKI GCWGC 4 cut(s) 67, 893, 908, 1106
AspLEI GCGC 3 cut(s) 179, 580, 969
AspS9I GGNCC 3 cut(s) 82, 212, 1045
AsuHPI GGTGA 4 cut(s) 62, 226, 557, 659
AsuII TTCGAA 1 cut(s) 760
AvaII GGWCC 3 cut(s) 82, 212, 1045
BalI TGGCCA 1 cut(s) 486
BanI GGYRCC 1 cut(s) 966
BbvI GCAGC 4 cut(s) 54, 880, 895, 1118
BccI CCATC 6 cut(s) 56, 355, 413, 476, 797, 905
BceAI ACGGC 5 cut(s) 10, 70, 217, 476, 821
BcgI CGANNNNNNTGC 4 cut(s) 729, 763, 890, 924
BciT130I CCWGG 1 cut(s) 488
BcoDI GTCTC 3 cut(s) 637, 812, 853
BcuI ACTAGT 1 cut(s) 1067
BfaI CTAG 2 cut(s) 1007, 1068
BfoI RGCGCY 1 cut(s) 970
BisI GCNGC 7 cut(s) 58, 68, 368, 371, 894, 909, 1107
BlsI GCNGC 7 cut(s) 59, 69, 369, 372, 895, 910, 1108
Bme1390I CCNGG 1 cut(s) 488
Bme18I GGWCC 3 cut(s) 82, 212, 1045
BmgT120I GGNCC 3 cut(s) 82, 212, 1045
BmiI GGNNCC 1 cut(s) 968
BmrFI CCNGG 1 cut(s) 488
BmsI GCATC 3 cut(s) 307, 485, 565
BpmI CTGGAG 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 760
BpuEI CTTGAG 2 cut(s) 71, 619
BsaAI YACGTR 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 967
BsaI GGTCTC 2 cut(s) 637, 812
BsaJI CCNNGG 2 cut(s) 205, 492
Bsc4I CCNNNNNNNGG 3 cut(s) 493, 866, 1034
Bse1I ACTGG 1 cut(s) 697
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 2 cut(s) 205, 492
BseGI GGATG 3 cut(s) 301, 366, 487
BseLI CCNNNNNNNGG 3 cut(s) 493, 866, 1034
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 1 cut(s) 697
BseRI GAGGAG 2 cut(s) 341, 344
BseX3I CGGCCG 1 cut(s) 368
BseXI GCAGC 4 cut(s) 54, 880, 895, 1118
BsgI GTGCAG 1 cut(s) 1048
Bsh1285I CGRYCG 1 cut(s) 371
BshFI GGCC 5 cut(s) 25, 204, 370, 486, 491
BshNI GGYRCC 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 371
BslFI GGGAC 3 cut(s) 119, 168, 1058
BslI CCNNNNNNNGG 3 cut(s) 493, 866, 1034
BsmAI GTCTC 3 cut(s) 637, 812, 853
BsmBI CGTCTC 1 cut(s) 853
BsmFI GGGAC 3 cut(s) 119, 168, 1058
BsnI GGCC 5 cut(s) 25, 204, 370, 486, 491
Bso31I GGTCTC 2 cut(s) 637, 812
Bsp119I TTCGAA 1 cut(s) 760
Bsp143I GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 8 cut(s) 48, 57, 210, 226, 241, 367, 371, 505
BspANI GGCC 5 cut(s) 25, 204, 370, 486, 491
BspCNI CTCAG 1 cut(s) 661
BspLI GGNNCC 1 cut(s) 968
BspPI GGATC 5 cut(s) 156, 397, 525, 567, 1123
BspT104I TTCGAA 1 cut(s) 760
BspT107I GGYRCC 1 cut(s) 966
BspTNI GGTCTC 2 cut(s) 637, 812
BsrBI CCGCTC 1 cut(s) 505
BsrI ACTGG 1 cut(s) 697
BssECI CCNNGG 2 cut(s) 205, 492
BssMI GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
BssNAI GTATAC 1 cut(s) 594
BssNI GRCGYC 1 cut(s) 967
BssT1I CCWWGG 1 cut(s) 205
Bst1107I GTATAC 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 3 cut(s) 288, 747, 991
Bst6I CTCTTC 3 cut(s) 103, 251, 695
BstACI GRCGYC 1 cut(s) 967
BstAPI GCANNNNNTGC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 760
BstDEI CTNAG 1 cut(s) 669
BstDSI CCRYGG 2 cut(s) 205, 492
BstF5I GGATG 3 cut(s) 301, 366, 487
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 3 cut(s) 179, 580, 969
BstKTI GATC 7 cut(s) 151, 356, 392, 520, 562, 1012, 1118
BstMAI GTCTC 3 cut(s) 637, 812, 853
BstMBI GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
BstMCI CGRYCG 1 cut(s) 371
BstMWI GCNNNNNNNGC 6 cut(s) 54, 73, 137, 317, 497, 872
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 1 cut(s) 486
BstV1I GCAGC 4 cut(s) 54, 880, 895, 1118
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 594
BstZI CGGCCG 1 cut(s) 368
BsuRI GGCC 5 cut(s) 25, 204, 370, 486, 491
BtgI CCRYGG 2 cut(s) 205, 492
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 301, 366, 487
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 3 cut(s) 126, 537, 987
CciNI GCGGCCGC 1 cut(s) 368
CfoI GCGC 3 cut(s) 179, 580, 969
Cfr13I GGNCC 3 cut(s) 82, 212, 1045
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 6 cut(s) 79, 206, 357, 599, 781, 1134
DdeI CTNAG 1 cut(s) 669
DinI GGCGCC 1 cut(s) 968
DpnI GATC 7 cut(s) 150, 355, 391, 519, 561, 1011, 1117
DpnII GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
DraI TTTAAA 1 cut(s) 720
DraIII CACNNNGTG 1 cut(s) 586
EaeI YGGCCR 3 cut(s) 202, 368, 484
EagI CGGCCG 1 cut(s) 368
Eam1104I CTCTTC 3 cut(s) 103, 251, 695
EarI CTCTTC 3 cut(s) 103, 251, 695
EclXI CGGCCG 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 205
Eco31I GGTCTC 2 cut(s) 637, 812
Eco32I GATATC 2 cut(s) 683, 985
Eco47I GGWCC 3 cut(s) 82, 212, 1045
Eco52I CGGCCG 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 237, 366
EcoRII CCWGG 1 cut(s) 486
EcoRV GATATC 2 cut(s) 683, 985
EcoT14I CCWWGG 1 cut(s) 205
EgeI GGCGCC 1 cut(s) 968
EheI GGCGCC 1 cut(s) 968
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 853
FaeI CATG 6 cut(s) 82, 209, 360, 602, 784, 1137
FaqI GGGAC 3 cut(s) 119, 168, 1058
FatI CATG 6 cut(s) 78, 205, 356, 598, 780, 1133
FauI CCCGC 1 cut(s) 233
FblI GTMKAC 3 cut(s) 512, 593, 654
Fnu4HI GCNGC 7 cut(s) 58, 68, 368, 371, 894, 909, 1107
FokI GGATG 3 cut(s) 308, 373, 494
Fsp4HI GCNGC 7 cut(s) 58, 68, 368, 371, 894, 909, 1107
FspAI RTGCGCAY 1 cut(s) 579
FspBI CTAG 2 cut(s) 1007, 1068
FspI TGCGCA 1 cut(s) 579
GlaI GCGC 3 cut(s) 178, 579, 968
GluI GCNGC 7 cut(s) 58, 68, 368, 371, 894, 909, 1107
GsuI CTGGAG 1 cut(s) 164
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 5 cut(s) 25, 204, 370, 486, 491
HhaI GCGC 3 cut(s) 179, 580, 969
Hin1I GRCGYC 1 cut(s) 967
Hin1II CATG 6 cut(s) 82, 209, 360, 602, 784, 1137
Hin6I GCGC 3 cut(s) 177, 578, 967
HinP1I GCGC 3 cut(s) 177, 578, 967
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 2 cut(s) 192, 449
HphI GGTGA 4 cut(s) 62, 226, 557, 659
Hpy166II GTNNAC 5 cut(s) 165, 284, 513, 594, 655
Hpy188I TCNGA 4 cut(s) 21, 292, 446, 481
Hpy188III TCNNGA 3 cut(s) 143, 215, 997
Hpy8I GTNNAC 5 cut(s) 165, 284, 513, 594, 655
Hpy99I CGWCG 3 cut(s) 751, 905, 983
HpyAV CCTTC 5 cut(s) 262, 266, 301, 541, 1036
HpyCH4III ACNGT 3 cut(s) 288, 747, 991
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 8 cut(s) 119, 269, 320, 410, 416, 797, 911, 1065
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 73, 137, 317, 497, 872
HpyF3I CTNAG 1 cut(s) 669
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 967
Hsp92II CATG 6 cut(s) 82, 209, 360, 602, 784, 1137
HspAI GCGC 3 cut(s) 177, 578, 967
KasI GGCGCC 1 cut(s) 966
Kzo9I GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 4 cut(s) 54, 880, 895, 1118
LweI GCATC 3 cut(s) 307, 485, 565
MaeI CTAG 2 cut(s) 1007, 1068
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 50, 765
MalI GATC 7 cut(s) 150, 355, 391, 519, 561, 1011, 1117
MbiI CCGCTC 1 cut(s) 505
MboI GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
MboII GAAGA 5 cut(s) 90, 238, 554, 673, 682
MfeI CAATTG 1 cut(s) 411
MflI RGATCY 1 cut(s) 559
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 5 cut(s) 133, 411, 612, 923, 948
MluNI TGGCCA 1 cut(s) 486
Mly113I GGCGCC 1 cut(s) 967
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 2 cut(s) 9, 169
Mox20I TGGCCA 1 cut(s) 486
MscI TGGCCA 1 cut(s) 486
MseI TTAA 7 cut(s) 348, 555, 615, 675, 719, 821, 1059
MslI CAYNNNNRTG 3 cut(s) 570, 587, 822
Msp20I TGGCCA 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 488
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 488
MwoI GCNNNNNNNGC 6 cut(s) 54, 73, 137, 317, 497, 872
NarI GGCGCC 1 cut(s) 967
NcoI CCATGG 1 cut(s) 205
NdeII GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
NlaIII CATG 6 cut(s) 82, 209, 360, 602, 784, 1137
NlaIV GGNNCC 1 cut(s) 968
NmuCI GTSAC 1 cut(s) 50
NotI GCGGCCGC 1 cut(s) 368
NsbI TGCGCA 1 cut(s) 579
NspI RCATGY 1 cut(s) 784
NspV TTCGAA 1 cut(s) 760
PciI ACATGT 1 cut(s) 780
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 3 cut(s) 493, 866, 1034
PkrI GCNGC 7 cut(s) 59, 69, 369, 372, 895, 910, 1108
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 970
PpsI GAGTC 1 cut(s) 200
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 968
PspPI GGNCC 3 cut(s) 82, 212, 1045
PsuI RGATCY 1 cut(s) 559
RseI CAYNNNNRTG 3 cut(s) 570, 587, 822
SaqAI TTAA 7 cut(s) 348, 555, 615, 675, 719, 821, 1059
SatI GCNGC 7 cut(s) 58, 68, 368, 371, 894, 909, 1107
Sau3AI GATC 7 cut(s) 148, 353, 389, 517, 559, 1009, 1115
Sau96I GGNCC 3 cut(s) 82, 212, 1045
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 488
SfaNI GCATC 3 cut(s) 307, 485, 565
SfoI GGCGCC 1 cut(s) 968
SfuI TTCGAA 1 cut(s) 760
SinI GGWCC 3 cut(s) 82, 212, 1045
SmiMI CAYNNNNRTG 3 cut(s) 570, 587, 822
SmlI CTYRAG 2 cut(s) 86, 634
SmoI CTYRAG 2 cut(s) 86, 634
SpeI ACTAGT 1 cut(s) 1067
Sse9I AATT 5 cut(s) 133, 411, 612, 923, 948
SsiI CCGC 8 cut(s) 48, 57, 210, 226, 241, 367, 371, 505
SspDI GGCGCC 1 cut(s) 966
SspMI CTAG 2 cut(s) 1007, 1068
StyD4I CCNGG 1 cut(s) 486
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 3 cut(s) 288, 747, 991
TaiI ACGT 1 cut(s) 185
TaqI TCGA 6 cut(s) 113, 452, 749, 760, 900, 1080
TasI AATT 5 cut(s) 133, 411, 612, 923, 948
TauI GCSGC 3 cut(s) 60, 370, 373
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 7 cut(s) 348, 555, 615, 675, 719, 821, 1059
Tru9I TTAA 7 cut(s) 348, 555, 615, 675, 719, 821, 1059
TscAI CASTG 3 cut(s) 126, 537, 994
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 4 cut(s) 67, 893, 908, 1106
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 3 cut(s) 220, 843, 1109
TspGWI ACGGA 2 cut(s) 530, 1128
TspRI CASTG 3 cut(s) 126, 537, 994
Van91I CCANNNNNTGG 3 cut(s) 493, 866, 1034
VpaK11BI GGWCC 3 cut(s) 82, 212, 1045
XceI RCATGY 1 cut(s) 784
XmiI GTMKAC 3 cut(s) 512, 593, 654
XspI CTAG 2 cut(s) 1007, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.