Prupe.6G224900_v2.0.a1

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001678
Physical Location & Seq
Forward (+)
22937871 .. 22941130
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G224900.1

Sequence Viewer

Length: 1137 bp
ATGATGTTGGAGATGTGTCGGAGGCCATTGGAGAAGTGCTTTGGAGGAGGAGATGGTGGAGATGGGCTCCTTTGGCACATGGACCTCAAGCCCCACTCTTCTGGGGACTATTCGATCGCAGTGGTTCAAGCCAATTCGGCGTTGGAAGACCAGGGACAGGTGTTCACATCCCCTTTCGCCACGTATATTGGGGTCTATGATGGCCATGGTGGCCCTGAAGCTTCTCGCTTCATCACCAATCGCCTCTTCCCCTTCCTTCACAAGTTTTCAATTGAGCAAGGTGGACTGTCAGAGGATGTGATAAGGAAGGCATTTGATGCAACTGAGGAAGAGTTCTTGGACTTGGTTAAGGCATCATGGCCTGTCCGGCCGCAGATTGCTTCCGTAGGTTCATGTTGTTTGGTTGGCGCAATTTCAAATGGTGTTTTATATGTTGCTAATCTCGGAGATTCGAGGGCAGTTCTTGGCCGGAGAGCATCAGAGGGGCAGGCGGTTGTGGCAGAGCGATTGTCTACAGATCACAATGTTGCAGTGGAGGAAGTGAGGAAGGAGGTTAAGGATCTTCACCCTGATGATGCACACATTGTGGTGTACACTCGTGGAGTTTGGCGAATTAAGGGCATTATTCAGGTGTCAAGGTCAATTGGTGACGTCTACTTGAAGAAACCCGAGTTTAATAGAGATCCGCTCTTCCACCATTTCGGTATACCTGTTCCTTTAAAGAGGCCTGTTATGACAGCAGAACCCTCGATATTAGTTCGGAAGTTAGAGCCACAGGACATGTTTTTGATATTTGCAACAGATGGTCTCTGGGAGCATTTGAGTGATGAAGCAGCTGTTAAAATCGTCTCAAAGAATTCAAGAGTTGGAATAGCAAAGCGGTTGGTCAGAGCTGCGATTGAGGAGGCTGCAAAGAAAAGAGAATTGAGATATGAGGACATAAAAAGAATCGAAAAGGGGGTAAGGCGCCATTTCCATGATGACATCACCGTCATCGTGATATTCCTGGATCATTCACAAGTTTCCCCAAATGCTAGTTTGACAGATCCGAGCCTCTTCAACTGCACCAGTGTTCCTGTTGATATCTTCTCCATGAACGGCGATGAAGCCGATGTATCACTCCACACCATTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

379

Amino Acids

41.97

Weight (kDa)

5.97

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 989
AatII GACGTC 1 cut(s) 654
AccB1I GGYRCC 1 cut(s) 966
AccBSI CCGCTC 1 cut(s) 688
AccI GTMKAC 3 cut(s) 512, 654, 706
AciI CCGC 4 cut(s) 371, 491, 686, 880
AclWI GGATC 4 cut(s) 567, 677, 1017, 1040
AcoI YGGCCR 3 cut(s) 202, 368, 466
AcsI RAATTY 1 cut(s) 856
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 2 cut(s) 651, 967
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 157
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 6 cut(s) 128, 270, 417, 661, 861, 1060
AjnI CCWGG 2 cut(s) 150, 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 3 cut(s) 221, 836, 893
AluI AGCT 3 cut(s) 221, 836, 893
Alw26I GTCTC 2 cut(s) 812, 853
AlwI GGATC 4 cut(s) 567, 677, 1017, 1040
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 7 cut(s) 23, 202, 211, 359, 368, 466, 725
ApeKI GCWGC 3 cut(s) 833, 893, 908
ApoI RAATTY 1 cut(s) 856
AspLEI GCGC 2 cut(s) 410, 969
AspS9I GGNCC 2 cut(s) 82, 212
AsuHPI GGTGA 4 cut(s) 226, 557, 659, 979
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 204
BanI GGYRCC 1 cut(s) 966
BanII GRGCYC 1 cut(s) 69
BauI CACGAG 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 3 cut(s) 845, 880, 895
BccI CCATC 4 cut(s) 47, 56, 194, 797
BceAI ACGGC 1 cut(s) 1114
BcgI CGANNNNNNTGC 2 cut(s) 729, 763
BciT130I CCWGG 2 cut(s) 152, 1007
BcoDI GTCTC 2 cut(s) 812, 853
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 1 cut(s) 513
BfoI RGCGCY 1 cut(s) 970
BglI GCCNNNNNGGC 3 cut(s) 137, 210, 367
BisI GCNGC 4 cut(s) 371, 834, 894, 909
BlsI GCNGC 4 cut(s) 372, 835, 895, 910
Bme1390I CCNGG 2 cut(s) 152, 1007
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 2 cut(s) 82, 212
BmiI GGNNCC 2 cut(s) 68, 968
BmrFI CCNGG 2 cut(s) 152, 1007
BmsI GCATC 4 cut(s) 307, 362, 485, 565
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 4 cut(s) 51, 83, 672, 704
BpuEI CTTGAG 1 cut(s) 71
BsaAI YACGTR 1 cut(s) 183
BsaHI GRCGYC 2 cut(s) 651, 967
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 151, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 1068
BseBI CCWGG 2 cut(s) 152, 1007
BseDI CCNNGG 2 cut(s) 151, 205
BseGI GGATG 2 cut(s) 167, 301
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 1068
BseRI GAGGAG 3 cut(s) 60, 63, 917
BseX3I CGGCCG 1 cut(s) 368
BseXI GCAGC 3 cut(s) 845, 880, 895
BsgI GTGCAG 1 cut(s) 1048
Bsh1285I CGRYCG 2 cut(s) 117, 371
BshFI GGCC 7 cut(s) 25, 204, 213, 361, 370, 468, 727
BshNI GGYRCC 1 cut(s) 966
BsiEI CGRYCG 2 cut(s) 117, 371
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 2 cut(s) 367, 469
BslFI GGGAC 2 cut(s) 119, 168
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 2 cut(s) 812, 853
BsmBI CGTCTC 1 cut(s) 853
BsmFI GGGAC 2 cut(s) 119, 168
BsnI GGCC 7 cut(s) 25, 204, 213, 361, 370, 468, 727
Bso31I GGTCTC 1 cut(s) 812
BsoBI CYCGRG 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
Bsp19I CCATGG 1 cut(s) 205
BspACI CCGC 4 cut(s) 371, 491, 686, 880
BspANI GGCC 7 cut(s) 25, 204, 213, 361, 370, 468, 727
BspCNI CTCAG 1 cut(s) 316
BspLI GGNNCC 2 cut(s) 68, 968
BspPI GGATC 4 cut(s) 567, 677, 1017, 1040
BspQI GCTCTTC 1 cut(s) 695
BspT107I GGYRCC 1 cut(s) 966
BspTNI GGTCTC 1 cut(s) 812
BsrBI CCGCTC 1 cut(s) 688
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 1 cut(s) 1068
BssECI CCNNGG 2 cut(s) 151, 205
BssMI GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
BssNAI GTATAC 1 cut(s) 707
BssNI GRCGYC 2 cut(s) 651, 967
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 205
Bst1107I GTATAC 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 2 cut(s) 152, 1007
Bst4CI ACNGT 2 cut(s) 288, 991
Bst6I CTCTTC 5 cut(s) 103, 251, 324, 695, 1061
BstACI GRCGYC 2 cut(s) 651, 967
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 205
BstF5I GGATG 2 cut(s) 167, 301
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 2 cut(s) 410, 969
BstKTI GATC 6 cut(s) 117, 520, 562, 685, 1012, 1048
BstMAI GTCTC 2 cut(s) 812, 853
BstMBI GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
BstMCI CGRYCG 2 cut(s) 117, 371
BstMWI GCNNNNNNNGC 6 cut(s) 73, 137, 210, 317, 367, 497
BstNI CCWGG 2 cut(s) 152, 1007
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 150, 1005
BstSFI CTRYAG 1 cut(s) 513
BstV1I GCAGC 3 cut(s) 845, 880, 895
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 3 cut(s) 559, 682, 1045
BstXI CCANNNNNNTGG 1 cut(s) 101
BstYI RGATCY 3 cut(s) 559, 682, 1045
BstZ17I GTATAC 1 cut(s) 707
BstZI CGGCCG 1 cut(s) 368
BsuRI GGCC 7 cut(s) 25, 204, 213, 361, 370, 468, 727
BtgI CCRYGG 1 cut(s) 205
BtgZI GCGATG 1 cut(s) 1116
BtsCI GGATG 2 cut(s) 167, 301
BtsI GCAGTG 2 cut(s) 126, 537
BtsIMutI CAGTG 3 cut(s) 126, 537, 1075
Cac8I GCNNGC 1 cut(s) 489
CfoI GCGC 2 cut(s) 410, 969
Cfr13I GGNCC 2 cut(s) 82, 212
Csp6I GTAC 1 cut(s) 592
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 7 cut(s) 79, 206, 357, 393, 781, 977, 1093
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 324
DinI GGCGCC 1 cut(s) 968
DpnI GATC 6 cut(s) 116, 519, 561, 684, 1011, 1047
DpnII GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
DraI TTTAAA 1 cut(s) 720
DraIII CACNNNGTG 1 cut(s) 586
DrdI GACNNNNNNGTC 1 cut(s) 989
DseDI GACNNNNNNGTC 1 cut(s) 989
EaeI YGGCCR 3 cut(s) 202, 368, 466
EagI CGGCCG 1 cut(s) 368
Eam1104I CTCTTC 5 cut(s) 103, 251, 324, 695, 1061
EarI CTCTTC 5 cut(s) 103, 251, 324, 695, 1061
EclXI CGGCCG 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 727
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 812
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 2 cut(s) 150, 1005
EcoRV GATATC 1 cut(s) 1084
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 69
EgeI GGCGCC 1 cut(s) 968
EheI GGCGCC 1 cut(s) 968
ErhI CCWWGG 1 cut(s) 205
Esp3I CGTCTC 1 cut(s) 853
FaeI CATG 7 cut(s) 82, 209, 360, 396, 784, 980, 1096
FaqI GGGAC 2 cut(s) 119, 168
FatI CATG 7 cut(s) 78, 205, 356, 392, 780, 976, 1092
FblI GTMKAC 3 cut(s) 512, 654, 706
Fnu4HI GCNGC 4 cut(s) 371, 834, 894, 909
FokI GGATG 2 cut(s) 154, 308
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 371, 834, 894, 909
FspBI CTAG 1 cut(s) 1035
GlaI GCGC 2 cut(s) 409, 968
GluI GCNGC 4 cut(s) 371, 834, 894, 909
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 7 cut(s) 25, 204, 213, 361, 370, 468, 727
HapII CCGG 2 cut(s) 367, 469
HhaI GCGC 2 cut(s) 410, 969
Hin1I GRCGYC 2 cut(s) 651, 967
Hin1II CATG 7 cut(s) 82, 209, 360, 396, 784, 980, 1096
Hin6I GCGC 2 cut(s) 408, 967
HinP1I GCGC 2 cut(s) 408, 967
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 2 cut(s) 449, 948
HpaII CCGG 2 cut(s) 367, 469
HphI GGTGA 4 cut(s) 226, 557, 659, 979
Hpy166II GTNNAC 7 cut(s) 165, 284, 513, 592, 594, 655, 707
Hpy188I TCNGA 7 cut(s) 21, 292, 446, 481, 762, 890, 1050
Hpy188III TCNNGA 2 cut(s) 861, 997
Hpy8I GTNNAC 7 cut(s) 165, 284, 513, 592, 594, 655, 707
HpyAV CCTTC 4 cut(s) 262, 266, 301, 541
HpyCH4III ACNGT 2 cut(s) 288, 991
HpyCH4IV ACGT 2 cut(s) 182, 651
HpyCH4V TGCA 6 cut(s) 320, 530, 578, 797, 911, 1065
HpyF10VI GCNNNNNNNGC 6 cut(s) 73, 137, 210, 317, 367, 497
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 2 cut(s) 182, 651
Hsp92I GRCGYC 2 cut(s) 651, 967
Hsp92II CATG 7 cut(s) 82, 209, 360, 396, 784, 980, 1096
HspAI GCGC 2 cut(s) 408, 967
KasI GGCGCC 1 cut(s) 966
Kzo9I GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
LguI GCTCTTC 1 cut(s) 695
LmnI GCTCC 2 cut(s) 72, 814
Lsp1109I GCAGC 3 cut(s) 845, 880, 895
LweI GCATC 4 cut(s) 307, 362, 485, 565
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 2 cut(s) 182, 651
MaeIII GTNAC 1 cut(s) 647
MalI GATC 6 cut(s) 116, 519, 561, 684, 1011, 1047
MbiI CCGCTC 1 cut(s) 688
MboI GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
MboII GAAGA 9 cut(s) 90, 158, 238, 341, 554, 673, 682, 1048, 1078
MfeI CAATTG 2 cut(s) 270, 642
MflI RGATCY 3 cut(s) 559, 682, 1045
MhlI GDGCHC 1 cut(s) 69
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 7 cut(s) 133, 270, 411, 612, 642, 856, 923
MluNI TGGCCA 1 cut(s) 204
Mly113I GGCGCC 1 cut(s) 967
MmeI TCCRAC 2 cut(s) 123, 847
Mox20I TGGCCA 1 cut(s) 204
MscI TGGCCA 1 cut(s) 204
MseI TTAA 6 cut(s) 348, 555, 615, 675, 719, 840
MslI CAYNNNNRTG 4 cut(s) 570, 587, 822, 975
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 2 cut(s) 367, 469
MspR9I CCNGG 2 cut(s) 152, 1007
MunI CAATTG 2 cut(s) 270, 642
MvaI CCWGG 2 cut(s) 152, 1007
MwoI GCNNNNNNNGC 6 cut(s) 73, 137, 210, 317, 367, 497
NarI GGCGCC 1 cut(s) 967
NcoI CCATGG 1 cut(s) 205
NdeII GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
NlaIII CATG 7 cut(s) 82, 209, 360, 396, 784, 980, 1096
NlaIV GGNNCC 2 cut(s) 68, 968
NmuCI GTSAC 1 cut(s) 647
NspI RCATGY 1 cut(s) 784
PceI AGGCCT 1 cut(s) 727
PciI ACATGT 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 695
PfeI GAWTC 2 cut(s) 449, 948
PfoI TCCNGGA 1 cut(s) 1005
PkrI GCNGC 4 cut(s) 372, 835, 895, 910
Ple19I CGATCG 1 cut(s) 117
PluTI GGCGCC 1 cut(s) 970
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 150, 1005
PspGI CCWGG 2 cut(s) 150, 1005
PspN4I GGNNCC 2 cut(s) 68, 968
PspPI GGNCC 2 cut(s) 82, 212
PsuI RGATCY 3 cut(s) 559, 682, 1045
PvuI CGATCG 1 cut(s) 117
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 4 cut(s) 570, 587, 822, 975
SapI GCTCTTC 1 cut(s) 695
SaqAI TTAA 6 cut(s) 348, 555, 615, 675, 719, 840
SatI GCNGC 4 cut(s) 371, 834, 894, 909
Sau3AI GATC 6 cut(s) 114, 517, 559, 682, 1009, 1045
Sau96I GGNCC 2 cut(s) 82, 212
ScrFI CCNGG 2 cut(s) 152, 1007
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 4 cut(s) 307, 362, 485, 565
SfcI CTRYAG 1 cut(s) 513
SfiI GGCCNNNNNGGCC 2 cut(s) 210, 367
SfoI GGCGCC 1 cut(s) 968
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 4 cut(s) 570, 587, 822, 975
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
Sse9I AATT 7 cut(s) 133, 270, 411, 612, 642, 856, 923
SseBI AGGCCT 1 cut(s) 727
SsiI CCGC 4 cut(s) 371, 491, 686, 880
SspDI GGCGCC 1 cut(s) 966
SspMI CTAG 1 cut(s) 1035
StuI AGGCCT 1 cut(s) 727
StyD4I CCNGG 2 cut(s) 150, 1005
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 288, 991
TaiI ACGT 2 cut(s) 185, 654
TaqI TCGA 4 cut(s) 113, 452, 749, 951
TasI AATT 7 cut(s) 133, 270, 411, 612, 642, 856, 923
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 2 cut(s) 449, 948
Tru1I TTAA 6 cut(s) 348, 555, 615, 675, 719, 840
Tru9I TTAA 6 cut(s) 348, 555, 615, 675, 719, 840
TscAI CASTG 3 cut(s) 126, 537, 1075
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 3 cut(s) 833, 893, 908
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 5 cut(s) 220, 381, 843, 1109, 1119
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 3 cut(s) 126, 537, 1075
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 856
XceI RCATGY 1 cut(s) 784
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmiI GTMKAC 3 cut(s) 512, 654, 706
XspI CTAG 1 cut(s) 1035
ZraI GACGTC 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.