Rroxscaffold_2G00136460

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001678
Physical Location & Seq
Forward (+)
74169276 .. 74172841
3566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136460.1

Sequence Viewer

Length: 1257 bp
ATGTTGGAGATGTGTAGGAGGCCATTGGAGAAGTGCTTTGGAGGTGGAGATGATGAGCTTCTATGGCACACGGACTTGAAGCCTCACTCCTCTGGGGAGTATTCAATTGCTGTTGTTCAGGCTAATTCATCTTTGGAAGATCAGGGACAAGTGTTCACCTCTCCTTCTGCCACGTATGTTGGAGTATATGACGGCCATGGTGGTCCTGAAGCTTCTAGATTCATCACTCAGCGGCTCTTCCCCTTTATTCAGAAGTTTTCAAATGAGCAAGGTGGACTATCAGAGGATGTGATTAGGAAAGCGTTTGATGCAACTGAGGAGGAGTTTGTGGACTTGGTCAAGCGGTCATGGCCGGCACGGCCAAGTATTGCTTCTGTGGGTTCATGCTGTCTTGTTGGCGCCATTACGAATGATGTCTTGTATGTGGCTAATCTCGGAGATTCGAGGGCGGTTCTTGGTCGGAGAGCAATAGATGGACCGACGGTGGTGGCAGAGCGGTTGTCGACAGATCACAATGTTGGAGTGGAGGAGGTGAGGAGGGAGGTTAAGGATCTTCACCCTGACGATTCGCACATTGTGGTTTATACTCGTGGGGTTTGGCGAATTAAGGGCATTATTCAGTTTGTTGTAAAATATGTGATTCGTGCTTGGTCTGGTCCTGTTATGATTTGCTCCAATGCTAGTAGGTTTTCCATCACTGAATTAGCATTTGGGGGAATTCGTCCCCAAGATTTTGTGAAATTTTGTGATATGAGCATTGTCTCTAGATCAATCGGAGATGTCTACTTGAAGAAACCTGAGTTTAACAGAGATCCTCTCTTCCAGTTTGGTATACCTGTTCCTTTAAAGAGGCCTGTAATGACATCAGTACCCTCAATATTGGTTCGGAAGTTGCAACCCCAGGACATGTTTTTGATATTTGCAACAGATGGTCTCTGGGAGCATTTGACTGATGAAGCAGCTGTTAAAATCGTCTCAAAAAATTCAAGAGTTGGAATAGCAAAGCGACTGGTAAGAACTGCCATCGAGGAGGCTGCAAGGAAAAGAGAATTGAAATATGATGACATAAAGAAACTTGAAAAGGGGGTAAGGCGCCATTTCCATGATGATATCACTGTAATTGTGATATTTCTGGATCATCCACAAACTTCCCCAAATGCTATGGATAACAGCCTCTTTACCTGCACTAGTGTTCCTGTTGATATCTTTTCTAATAATGATGATGAAGCAGTTGGCTCACTTCATACATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

418

Amino Acids

46.71

Weight (kDa)

6.15

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 55 - 347 4.7e-32 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1190
AccB1I GGYRCC 2 cut(s) 398, 1092
AccBSI CCGCTC 1 cut(s) 496
AccI GTMKAC 3 cut(s) 503, 783, 832
AciI CCGC 4 cut(s) 232, 343, 449, 496
AclWI GGATC 3 cut(s) 558, 806, 1143
AcoI YGGCCR 3 cut(s) 193, 350, 359
AcsI RAATTY 3 cut(s) 717, 740, 982
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 2 cut(s) 399, 1093
AdeI CACNNNGTG 1 cut(s) 577
AfaI GTAC 1 cut(s) 870
AflIII ACRYGT 1 cut(s) 906
AgsI TTSAA 7 cut(s) 79, 105, 261, 790, 987, 1054, 1079
AhlI ACTAGT 1 cut(s) 1187
AjnI CCWGG 1 cut(s) 900
AjuI GAANNNNNNNTTGG 4 cut(s) 355, 387, 693, 725
AluBI AGCT 3 cut(s) 58, 212, 962
AluI AGCT 3 cut(s) 58, 212, 962
Alw26I GTCTC 3 cut(s) 766, 938, 979
AlwI GGATC 3 cut(s) 558, 806, 1143
AoxI GGCC 5 cut(s) 20, 193, 350, 359, 851
ApeKI GCWGC 2 cut(s) 959, 1034
ApoI RAATTY 3 cut(s) 717, 740, 982
AspLEI GCGC 2 cut(s) 401, 1095
AspS9I GGNCC 3 cut(s) 203, 476, 656
AsuHPI GGTGA 3 cut(s) 148, 544, 548
AvaII GGWCC 3 cut(s) 203, 476, 656
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BanI GGYRCC 2 cut(s) 398, 1092
BauI CACGAG 1 cut(s) 588
BbvI GCAGC 2 cut(s) 971, 1021
BccI CCATC 4 cut(s) 467, 701, 923, 1031
BceAI ACGGC 2 cut(s) 208, 374
BcgI CGANNNNNNTGC 2 cut(s) 1016, 1050
BciT130I CCWGG 1 cut(s) 902
BcoDI GTCTC 3 cut(s) 766, 938, 979
BcuI ACTAGT 1 cut(s) 1187
BfaI CTAG 4 cut(s) 216, 681, 765, 1188
BfoI RGCGCY 2 cut(s) 402, 1096
BfuAI ACCTGC 1 cut(s) 1190
BglI GCCNNNNNGGC 1 cut(s) 358
BisI GCNGC 3 cut(s) 233, 960, 1035
BlsI GCNGC 3 cut(s) 234, 961, 1036
Bme1390I CCNGG 1 cut(s) 902
Bme18I GGWCC 3 cut(s) 203, 476, 656
BmgT120I GGNCC 3 cut(s) 203, 476, 656
BmiI GGNNCC 2 cut(s) 400, 1094
BmrFI CCNGG 1 cut(s) 902
BmsI GCATC 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 801, 833
BsaAI YACGTR 1 cut(s) 174
BsaHI GRCGYC 2 cut(s) 399, 1093
BsaI GGTCTC 1 cut(s) 938
BsaJI CCNNGG 2 cut(s) 196, 900
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 823, 1014
BseBI CCWGG 1 cut(s) 902
BseDI CCNNGG 2 cut(s) 196, 900
BseGI GGATG 2 cut(s) 292, 1138
BseMII CTCAG 3 cut(s) 242, 306, 789
BseNI ACTGG 2 cut(s) 823, 1014
BseRI GAGGAG 6 cut(s) 79, 332, 335, 542, 550, 1043
BseXI GCAGC 2 cut(s) 971, 1021
BsgI GTGCAG 1 cut(s) 1168
BshFI GGCC 5 cut(s) 22, 195, 352, 361, 853
BshNI GGYRCC 2 cut(s) 398, 1092
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 2 cut(s) 159, 708
BsmAI GTCTC 3 cut(s) 766, 938, 979
BsmBI CGTCTC 1 cut(s) 979
BsmFI GGGAC 2 cut(s) 159, 708
BsnI GGCC 5 cut(s) 22, 195, 352, 361, 853
Bso31I GGTCTC 1 cut(s) 938
Bsp143I GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 4 cut(s) 232, 343, 449, 496
BspANI GGCC 5 cut(s) 22, 195, 352, 361, 853
BspCNI CTCAG 3 cut(s) 241, 307, 790
BspLI GGNNCC 2 cut(s) 400, 1094
BspMI ACCTGC 1 cut(s) 1190
BspPI GGATC 3 cut(s) 558, 806, 1143
BspQI GCTCTTC 1 cut(s) 242
BspT107I GGYRCC 2 cut(s) 398, 1092
BspTNI GGTCTC 1 cut(s) 938
BsrBI CCGCTC 1 cut(s) 496
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 2 cut(s) 823, 1014
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 196, 900
BssMI GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
BssNAI GTATAC 1 cut(s) 833
BssNI GRCGYC 2 cut(s) 399, 1093
BssSI CACGAG 1 cut(s) 588
BssT1I CCWWGG 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 833
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 902
Bst4CI ACNGT 2 cut(s) 484, 1117
Bst6I CTCTTC 2 cut(s) 242, 824
BstACI GRCGYC 2 cut(s) 399, 1093
BstBAI YACGTR 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 3 cut(s) 228, 315, 798
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 2 cut(s) 292, 1138
BstH2I RGCGCY 2 cut(s) 402, 1096
BstHHI GCGC 2 cut(s) 401, 1095
BstKTI GATC 6 cut(s) 142, 511, 553, 770, 814, 1138
BstMAI GTCTC 3 cut(s) 766, 938, 979
BstMBI GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
BstMWI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
BstNI CCWGG 1 cut(s) 902
BstNSI RCATGY 1 cut(s) 910
BstSCI CCNGG 1 cut(s) 900
BstV1I GCAGC 2 cut(s) 971, 1021
BstX2I RGATCY 2 cut(s) 550, 811
BstYI RGATCY 2 cut(s) 550, 811
BstZ17I GTATAC 1 cut(s) 833
BsuRI GGCC 5 cut(s) 22, 195, 352, 361, 853
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 2 cut(s) 292, 1138
BtsIMutI CAGTG 2 cut(s) 696, 1113
BveI ACCTGC 1 cut(s) 1190
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 2 cut(s) 401, 1095
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 3 cut(s) 203, 476, 656
Csp6I GTAC 1 cut(s) 869
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 5 cut(s) 197, 348, 384, 907, 1103
CviQI GTAC 1 cut(s) 869
DdeI CTNAG 3 cut(s) 228, 315, 798
DinI GGCGCC 2 cut(s) 400, 1094
DpnI GATC 6 cut(s) 141, 510, 552, 769, 813, 1137
DpnII GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
DraI TTTAAA 1 cut(s) 846
DraIII CACNNNGTG 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 193, 350, 359
Eam1104I CTCTTC 2 cut(s) 242, 824
EarI CTCTTC 2 cut(s) 242, 824
Eco130I CCWWGG 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 853
Eco31I GGTCTC 1 cut(s) 938
Eco32I GATATC 2 cut(s) 1111, 1204
Eco47I GGWCC 3 cut(s) 203, 476, 656
Eco57I CTGAAG 1 cut(s) 228
EcoRI GAATTC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 900
EcoRV GATATC 2 cut(s) 1111, 1204
EcoT14I CCWWGG 1 cut(s) 196
EgeI GGCGCC 2 cut(s) 400, 1094
EheI GGCGCC 2 cut(s) 400, 1094
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 979
FaeI CATG 5 cut(s) 200, 351, 387, 910, 1106
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 2 cut(s) 159, 708
FatI CATG 5 cut(s) 196, 347, 383, 906, 1102
FblI GTMKAC 3 cut(s) 503, 783, 832
Fnu4HI GCNGC 3 cut(s) 233, 960, 1035
FokI GGATG 2 cut(s) 299, 1125
Fsp4HI GCNGC 3 cut(s) 233, 960, 1035
FspBI CTAG 4 cut(s) 216, 681, 765, 1188
GlaI GCGC 2 cut(s) 400, 1094
GluI GCNGC 3 cut(s) 233, 960, 1035
HaeII RGCGCY 2 cut(s) 402, 1096
HaeIII GGCC 5 cut(s) 22, 195, 352, 361, 853
HapII CCGG 1 cut(s) 353
HhaI GCGC 2 cut(s) 401, 1095
Hin1I GRCGYC 2 cut(s) 399, 1093
Hin1II CATG 5 cut(s) 200, 351, 387, 910, 1106
Hin6I GCGC 2 cut(s) 399, 1093
HinP1I GCGC 2 cut(s) 399, 1093
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HindIII AAGCTT 1 cut(s) 210
HinfI GANTC 4 cut(s) 219, 440, 566, 640
HpaII CCGG 1 cut(s) 353
HphI GGTGA 3 cut(s) 148, 544, 548
Hpy166II GTNNAC 6 cut(s) 156, 275, 331, 504, 784, 833
Hpy188I TCNGA 6 cut(s) 252, 283, 437, 462, 776, 888
Hpy188III TCNNGA 5 cut(s) 206, 216, 765, 987, 1133
Hpy8I GTNNAC 6 cut(s) 156, 275, 331, 504, 784, 833
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 2 cut(s) 484, 1117
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 5 cut(s) 311, 895, 923, 1037, 1185
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
HpyF3I CTNAG 3 cut(s) 228, 315, 798
HpySE526I ACGT 1 cut(s) 173
Hsp92I GRCGYC 2 cut(s) 399, 1093
Hsp92II CATG 5 cut(s) 200, 351, 387, 910, 1106
HspAI GCGC 2 cut(s) 399, 1093
KasI GGCGCC 2 cut(s) 398, 1092
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
Kzo9I GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 2 cut(s) 677, 940
Lsp1109I GCAGC 2 cut(s) 971, 1021
LweI GCATC 1 cut(s) 298
MaeI CTAG 4 cut(s) 216, 681, 765, 1188
MaeII ACGT 1 cut(s) 173
MalI GATC 6 cut(s) 141, 510, 552, 769, 813, 1137
MbiI CCGCTC 1 cut(s) 496
MboI GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
MboII GAAGA 5 cut(s) 149, 229, 545, 802, 811
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 2 cut(s) 550, 811
MluCI AATT 9 cut(s) 105, 124, 603, 701, 717, 740, 982, 1049, 1119
Mly113I GGCGCC 2 cut(s) 399, 1093
MmeI TCCRAC 4 cut(s) 160, 440, 499, 973
MroNI GCCGGC 1 cut(s) 352
MseI TTAA 5 cut(s) 546, 606, 804, 845, 966
MslI CAYNNNNRTG 1 cut(s) 1101
MspA1I CMGCKG 2 cut(s) 232, 962
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 902
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 902
MwoI GCNNNNNNNGC 4 cut(s) 64, 308, 349, 358
NaeI GCCGGC 1 cut(s) 354
NarI GGCGCC 2 cut(s) 399, 1093
NcoI CCATGG 1 cut(s) 196
NdeII GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 5 cut(s) 200, 351, 387, 910, 1106
NlaIV GGNNCC 2 cut(s) 400, 1094
NspI RCATGY 1 cut(s) 910
PceI AGGCCT 1 cut(s) 853
PciI ACATGT 1 cut(s) 906
PciSI GCTCTTC 1 cut(s) 242
PdiI GCCGGC 1 cut(s) 354
PfeI GAWTC 4 cut(s) 219, 440, 566, 640
PflFI GACNNNGTC 1 cut(s) 335
PkrI GCNGC 3 cut(s) 234, 961, 1036
PluTI GGCGCC 2 cut(s) 402, 1096
Ppu21I YACGTR 1 cut(s) 174
PscI ACATGT 1 cut(s) 906
Psp6I CCWGG 1 cut(s) 900
PspGI CCWGG 1 cut(s) 900
PspN4I GGNNCC 2 cut(s) 400, 1094
PspPI GGNCC 3 cut(s) 203, 476, 656
PsuI RGATCY 2 cut(s) 550, 811
PsyI GACNNNGTC 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 1 cut(s) 870
RsaNI GTAC 1 cut(s) 869
RseI CAYNNNNRTG 1 cut(s) 1101
SalI GTCGAC 1 cut(s) 502
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 5 cut(s) 546, 606, 804, 845, 966
SatI GCNGC 3 cut(s) 233, 960, 1035
Sau3AI GATC 6 cut(s) 139, 508, 550, 767, 811, 1135
Sau96I GGNCC 3 cut(s) 203, 476, 656
ScrFI CCNGG 1 cut(s) 902
SfaNI GCATC 1 cut(s) 298
SfiI GGCCNNNNNGGCC 1 cut(s) 358
SfoI GGCGCC 2 cut(s) 400, 1094
SinI GGWCC 3 cut(s) 203, 476, 656
SmiMI CAYNNNNRTG 1 cut(s) 1101
SpeI ACTAGT 1 cut(s) 1187
Sse9I AATT 9 cut(s) 105, 124, 603, 701, 717, 740, 982, 1049, 1119
SseBI AGGCCT 1 cut(s) 853
SsiI CCGC 4 cut(s) 232, 343, 449, 496
SspDI GGCGCC 2 cut(s) 398, 1092
SspI AATATT 1 cut(s) 879
SspMI CTAG 4 cut(s) 216, 681, 765, 1188
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 1 cut(s) 900
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 2 cut(s) 484, 1117
TaiI ACGT 1 cut(s) 176
TaqI TCGA 3 cut(s) 443, 503, 1026
TaqII GACCGA 1 cut(s) 493
TasI AATT 9 cut(s) 105, 124, 603, 701, 717, 740, 982, 1049, 1119
TauI GCSGC 1 cut(s) 235
TfiI GAWTC 4 cut(s) 219, 440, 566, 640
Tru1I TTAA 5 cut(s) 546, 606, 804, 845, 966
Tru9I TTAA 5 cut(s) 546, 606, 804, 845, 966
TscAI CASTG 2 cut(s) 703, 1120
TseI GCWGC 2 cut(s) 959, 1034
TspDTI ATGAA 6 cut(s) 117, 211, 372, 969, 1232, 1239
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 2 cut(s) 703, 1120
Tth111I GACNNNGTC 1 cut(s) 335
VpaK11BI GGWCC 3 cut(s) 203, 476, 656
XapI RAATTY 3 cut(s) 717, 740, 982
XbaI TCTAGA 2 cut(s) 215, 764
XceI RCATGY 1 cut(s) 910
XmiI GTMKAC 3 cut(s) 503, 783, 832
XspI CTAG 4 cut(s) 216, 681, 765, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.