MD05G1095000.v1.1

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001678
Physical Location & Seq
Forward (+)
20115887 .. 20118407
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1095000.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGCTGCGCTCGTGCTACAGGCCGCTCGAGCGCTGTCTAGGGTTGCGATTTGGTGCTGGAGGCGGCGGAGATGGGCTTTGGCACATGGACTTGAAGCCCCACGCCTCCGGTGACTACTCGATTGCTGTGGTTCAGGCCAATTCGAATTTGGAGGATCAAGGTCAGGTCTTCACCTCGCCCTCTGCGACGTATGTTGGTGTATATGACGGCCATGGCGGTCCCGAAGCTTCTAGATTCGTCAACAGTCATCTCTTCCCTTATCTACACAAATTTGCGACGGAGCAAGGTGGGTTGTCACCAGATGTGATAAAAAAGGCATTCAGCGCCACCGAGGAGGATTTTTTGCGTTTGGTGAAGCGATCTTTGCCGATGATGCCCCAAATCGCTTCCGTTGGGTCGTGTTGCCTTCTGGGAGCAATATCAAATGATGTTCTGTATGTAGCCAATCTGGGGGACTCGAGAGCAGTCCTTGGCCGGAGAGTTTCAGATAGTAAGAGGAGTCCAGTGGTGGCAGAACGCATGTCCACTGATCACAATGTGGGAGTTGAGGAGGTCAGGAAGGAGGTCGAAGCACTACATCCCGATGATTCACATGTTGTGGTGTTTACTCGTGGAGTTTGGAGGATAAAAGGCATAATTCAGGTGTCAAGATCTATTGGTGATGTTTATTTAAAGAAGCCTGAGTTTAACAGAGACCCCATATACCAGCAATTTGCGAACCCCATTCCTATGAAACGGCCAGTGATGACAGCAGAGCCCTCAATCATTACAAGGAAACTGAAGCCACAGGATATGTTTTTGATATTTGCCTCAGATGGCCTCTGGGAACAGCTGACTGATGACGCAGCGGTAGAAATTGTTTTCAAAAACCCTAGAGCTGGTATTGCAAAGAGATTAGTTAGAGCCGCTCTTCAAGAAGCTGCAAAAAAGAGGGAGATGAGATACAATGACATAAAGAAAATCGAAAAGGGGATAAGGCGACACTTCCACGATGACATCACTGTTATAGTAGTCTATCTTGATGACCACAAAAGCTCCTCAAAACGTAAATCTAAGAACATCATTCTTGGCTCTACCAGTGCCCCTGTTGATATTTTCTCGGTCAGTGCAGATAATCCGTATGAGCAGGATCTGCACAACATGATCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

384

Amino Acids

42.65

Weight (kDa)

8.86

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 309 1e-36 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 25, 908
AciI CCGC 6 cut(s) 23, 63, 66, 216, 848, 906
AclWI GGATC 2 cut(s) 162, 1137
AcoI YGGCCR 3 cut(s) 208, 472, 737
AcsI RAATTY 2 cut(s) 145, 269
AcuI CTGAAG 1 cut(s) 800
AdeI CACNNNGTG 1 cut(s) 538
AfeI AGCGCT 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 450, 878
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 3 cut(s) 94, 865, 914
AluBI AGCT 5 cut(s) 227, 832, 878, 920, 1035
AluI AGCT 5 cut(s) 227, 832, 878, 920, 1035
Alw26I GTCTC 1 cut(s) 687
AlwI GGATC 2 cut(s) 162, 1137
AlwNI CAGNNNCTG 1 cut(s) 1132
Ama87I CYCGRG 2 cut(s) 26, 457
Aor51HI AGCGCT 1 cut(s) 32
AoxI GGCC 6 cut(s) 20, 135, 208, 472, 737, 817
ApeKI GCWGC 3 cut(s) 4, 845, 920
ApoI RAATTY 2 cut(s) 145, 269
AspLEI GCGC 3 cut(s) 9, 33, 326
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 5 cut(s) 122, 163, 288, 364, 671
AsuII TTCGAA 1 cut(s) 143
AvaI CYCGRG 2 cut(s) 26, 457
AvaII GGWCC 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 759
BauI CACGAG 3 cut(s) 10, 609, 1147
BbsI GAAGAC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 857, 907
BccI CCATC 2 cut(s) 65, 809
BceAI ACGGC 2 cut(s) 223, 752
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 1 cut(s) 687
BfaI CTAG 3 cut(s) 38, 231, 873
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 2 cut(s) 34, 327
BglII AGATCT 1 cut(s) 650
BisI GCNGC 6 cut(s) 5, 23, 64, 846, 906, 921
BlsI GCNGC 6 cut(s) 6, 24, 65, 847, 907, 922
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 2 cut(s) 26, 457
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 220
BmsI GCATC 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 160
BpmI CTGGAG 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 687
BsaJI CCNNGG 3 cut(s) 211, 330, 469
BsaWI WCCGGW 1 cut(s) 107
BsaXI ACNNNNNCTCC 2 cut(s) 1019, 1049
Bsc4I CCNNNNNNNGG 2 cut(s) 450, 878
Bse1I ACTGG 3 cut(s) 503, 740, 1077
BseDI CCNNGG 3 cut(s) 211, 330, 469
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 2 cut(s) 450, 878
BseMII CTCAG 2 cut(s) 672, 825
BseNI ACTGG 3 cut(s) 503, 740, 1077
BseRI GAGGAG 4 cut(s) 347, 511, 563, 1027
BseSI GKGCMC 1 cut(s) 1084
BseXI GCAGC 2 cut(s) 857, 907
BsgI GTGCAG 2 cut(s) 1118, 1128
BshFI GGCC 6 cut(s) 22, 137, 210, 474, 739, 819
BsiHKCI CYCGRG 2 cut(s) 26, 457
BsiSI CCGG 2 cut(s) 108, 475
BslFI GGGAC 2 cut(s) 204, 467
BslI CCNNNNNNNGG 2 cut(s) 450, 878
BsmAI GTCTC 1 cut(s) 687
BsmFI GGGAC 2 cut(s) 204, 467
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 6 cut(s) 22, 137, 210, 474, 739, 819
Bso31I GGTCTC 1 cut(s) 687
BsoBI CYCGRG 2 cut(s) 26, 457
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 759, 1084
Bsp143I GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 6 cut(s) 23, 63, 66, 216, 848, 906
BspANI GGCC 6 cut(s) 22, 137, 210, 474, 739, 819
BspCNI CTCAG 2 cut(s) 673, 824
BspLI GGNNCC 1 cut(s) 220
BspPI GGATC 2 cut(s) 162, 1137
BspQI GCTCTTC 1 cut(s) 915
BspT104I TTCGAA 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 687
BsrBI CCGCTC 2 cut(s) 25, 908
BsrI ACTGG 3 cut(s) 503, 740, 1077
BssECI CCNNGG 3 cut(s) 211, 330, 469
BssMI GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
BssSI CACGAG 3 cut(s) 10, 609, 1147
BssT1I CCWWGG 2 cut(s) 211, 469
Bst2BI CACGAG 3 cut(s) 10, 609, 1147
Bst4CI ACNGT 2 cut(s) 245, 1003
Bst6I CTCTTC 2 cut(s) 257, 915
BstAPI GCANNNNNTGC 1 cut(s) 1132
BstBI TTCGAA 1 cut(s) 143
BstDEI CTNAG 3 cut(s) 681, 811, 1053
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 2 cut(s) 34, 327
BstHHI GCGC 3 cut(s) 9, 33, 326
BstKTI GATC 6 cut(s) 157, 362, 532, 653, 1132, 1146
BstMAI GTCTC 1 cut(s) 687
BstMBI GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
BstMWI GCNNNNNNNGC 6 cut(s) 28, 323, 364, 373, 884, 1132
BstNSI RCATGY 2 cut(s) 523, 596
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 1084
BstV1I GCAGC 2 cut(s) 857, 907
BstV2I GAAGAC 1 cut(s) 160
BstX2I RGATCY 2 cut(s) 650, 1129
BstYI RGATCY 2 cut(s) 650, 1129
BsuRI GGCC 6 cut(s) 22, 137, 210, 474, 739, 819
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 1 cut(s) 577
BtsIMutI CAGTG 6 cut(s) 510, 525, 747, 999, 1084, 1111
CaiI CAGNNNCTG 1 cut(s) 1132
CfoI GCGC 3 cut(s) 9, 33, 326
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 1 cut(s) 851
CviAII CATG 5 cut(s) 85, 212, 520, 593, 1141
DdeI CTNAG 3 cut(s) 681, 811, 1053
DpnI GATC 6 cut(s) 156, 361, 531, 652, 1131, 1145
DpnII GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
DraI TTTAAA 1 cut(s) 672
DraIII CACNNNGTG 1 cut(s) 538
EaeI YGGCCR 3 cut(s) 208, 472, 737
Eam1104I CTCTTC 2 cut(s) 257, 915
EarI CTCTTC 2 cut(s) 257, 915
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 211, 469
Eco24I GRGCYC 1 cut(s) 759
Eco31I GGTCTC 1 cut(s) 687
Eco47I GGWCC 1 cut(s) 218
Eco47III AGCGCT 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 800
Eco88I CYCGRG 2 cut(s) 26, 457
EcoT14I CCWWGG 2 cut(s) 211, 469
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 2 cut(s) 211, 469
FaeI CATG 5 cut(s) 88, 215, 523, 596, 1144
FaqI GGGAC 2 cut(s) 204, 467
FatI CATG 5 cut(s) 84, 211, 519, 592, 1140
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 6 cut(s) 5, 23, 64, 846, 906, 921
FokI GGATG 1 cut(s) 564
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 6 cut(s) 5, 23, 64, 846, 906, 921
FspBI CTAG 3 cut(s) 38, 231, 873
GlaI GCGC 3 cut(s) 8, 32, 325
GluI GCNGC 6 cut(s) 5, 23, 64, 846, 906, 921
GsuI CTGGAG 1 cut(s) 78
HaeII RGCGCY 2 cut(s) 34, 327
HaeIII GGCC 6 cut(s) 22, 137, 210, 474, 739, 819
HapII CCGG 2 cut(s) 108, 475
HgaI GACGC 1 cut(s) 851
HhaI GCGC 3 cut(s) 9, 33, 326
Hin1II CATG 5 cut(s) 88, 215, 523, 596, 1144
Hin6I GCGC 3 cut(s) 7, 31, 324
HinP1I GCGC 3 cut(s) 7, 31, 324
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 234, 455, 499, 587
HpaII CCGG 2 cut(s) 108, 475
HphI GGTGA 5 cut(s) 122, 163, 288, 364, 671
Hpy166II GTNNAC 3 cut(s) 241, 525, 606
Hpy188I TCNGA 2 cut(s) 487, 814
Hpy188III TCNNGA 9 cut(s) 221, 231, 459, 556, 581, 648, 914, 1019, 1149
Hpy8I GTNNAC 3 cut(s) 241, 525, 606
Hpy99I CGWCG 2 cut(s) 190, 280
HpyAV CCTTC 2 cut(s) 416, 553
HpyCH4III ACNGT 2 cut(s) 245, 1003
HpyCH4IV ACGT 2 cut(s) 188, 1045
HpyCH4V TGCA 4 cut(s) 887, 923, 1109, 1135
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 323, 364, 373, 884, 1132
HpyF3I CTNAG 3 cut(s) 681, 811, 1053
HpySE526I ACGT 2 cut(s) 188, 1045
Hsp92II CATG 5 cut(s) 88, 215, 523, 596, 1144
HspAI GCGC 3 cut(s) 7, 31, 324
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 3 cut(s) 280, 413, 1040
Lsp1109I GCAGC 2 cut(s) 857, 907
LweI GCATC 1 cut(s) 363
MaeI CTAG 3 cut(s) 38, 231, 873
MaeII ACGT 2 cut(s) 188, 1045
MaeIII GTNAC 2 cut(s) 110, 294
MalI GATC 6 cut(s) 156, 361, 531, 652, 1131, 1145
MbiI CCGCTC 2 cut(s) 25, 908
MboI GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
MboII GAAGA 3 cut(s) 160, 244, 902
MflI RGATCY 2 cut(s) 650, 1129
MhlI GDGCHC 2 cut(s) 759, 1084
MluCI AATT 6 cut(s) 139, 145, 269, 636, 710, 855
MlyI GAGTC 2 cut(s) 449, 508
MseI TTAA 2 cut(s) 671, 687
MslI CAYNNNNRTG 2 cut(s) 582, 728
MspA1I CMGCKG 2 cut(s) 832, 848
MspI CCGG 2 cut(s) 108, 475
Mva1269I GAATGC 1 cut(s) 317
MwoI GCNNNNNNNGC 6 cut(s) 28, 323, 364, 373, 884, 1132
NcoI CCATGG 1 cut(s) 211
NdeII GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
NlaIII CATG 5 cut(s) 88, 215, 523, 596, 1144
NlaIV GGNNCC 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 110, 294
NspI RCATGY 2 cut(s) 523, 596
NspV TTCGAA 1 cut(s) 143
PaeR7I CTCGAG 2 cut(s) 26, 457
PciI ACATGT 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 915
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 2 cut(s) 234, 587
PkrI GCNGC 6 cut(s) 6, 24, 65, 847, 907, 922
PleI GAGTC 2 cut(s) 449, 507
PpsI GAGTC 2 cut(s) 449, 507
PscI ACATGT 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 1 cut(s) 218
PspXI VCTCGAGB 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 1132
PsuI RGATCY 2 cut(s) 650, 1129
PvuII CAGCTG 1 cut(s) 832
RseI CAYNNNNRTG 2 cut(s) 582, 728
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 2 cut(s) 671, 687
SatI GCNGC 6 cut(s) 5, 23, 64, 846, 906, 921
Sau3AI GATC 6 cut(s) 154, 359, 529, 650, 1129, 1143
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 2 cut(s) 449, 508
SduI GDGCHC 2 cut(s) 759, 1084
SfaNI GCATC 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 16
Sfr274I CTCGAG 2 cut(s) 26, 457
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 1 cut(s) 218
SlaI CTCGAG 2 cut(s) 26, 457
SmiMI CAYNNNNRTG 2 cut(s) 582, 728
SmlI CTYRAG 2 cut(s) 26, 457
SmoI CTYRAG 2 cut(s) 26, 457
Sse9I AATT 6 cut(s) 139, 145, 269, 636, 710, 855
SsiI CCGC 6 cut(s) 23, 63, 66, 216, 848, 906
SspMI CTAG 3 cut(s) 38, 231, 873
StyI CCWWGG 2 cut(s) 211, 469
TaaI ACNGT 2 cut(s) 245, 1003
TaiI ACGT 2 cut(s) 191, 1048
TaqI TCGA 6 cut(s) 27, 119, 143, 458, 567, 963
TaqII GACCGA 1 cut(s) 1090
TasI AATT 6 cut(s) 139, 145, 269, 636, 710, 855
TauI GCSGC 3 cut(s) 25, 66, 908
TfiI GAWTC 2 cut(s) 234, 587
Tru1I TTAA 2 cut(s) 671, 687
Tru9I TTAA 2 cut(s) 671, 687
TscAI CASTG 6 cut(s) 510, 532, 747, 1006, 1084, 1111
TseFI GTSAC 2 cut(s) 110, 294
TseI GCWGC 3 cut(s) 4, 845, 920
Tsp45I GTSAC 2 cut(s) 110, 294
TspDTI ATGAA 1 cut(s) 746
TspGWI ACGGA 3 cut(s) 293, 379, 1107
TspRI CASTG 6 cut(s) 510, 532, 747, 1006, 1084, 1111
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 2 cut(s) 145, 269
XbaI TCTAGA 1 cut(s) 230
XceI RCATGY 2 cut(s) 523, 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XhoI CTCGAG 2 cut(s) 26, 457
XspI CTAG 3 cut(s) 38, 231, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.