Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001678

Physical Location & Seq

Genomic Coordinates Reverse (-)

16632027 .. 16634500

Gene Structure 2474 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1102000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGCTGCGCTCGTGCTACAGGCTGCTCGAGCGCTGTTTGAGGTTGCGATGTGGTGCTGGGGGCGGAGGCGATGGCCTTTGGCACATGGACTTGGAGTCCCACGCCTCCGGTGACTACTCGATCGCTGTGGTTCAGGCCAATTCGAATTTGGAGGATCAAGGTCAGGTCTTCACCTCGCCCTCTGCAACGTATGTTGGTGTTTATGATGGCCATGGCGGTCCCCAAGCTTCTAGATTCGTCAACAACCATCTCCTCCCATATTTACACAAATTTGCGATGGAACAAGGGGGATTGTCAGCAGATGTGATAAAGAAGGCATTCAGTGCCACTGAAGAAGATTTTTTGCATTTGGTGAAGCGATCTTTGCCAAAGATGCCCCAAATCGCTTCCGTTGGTTCGTGTTGCCTTGTGGGAGCAATATCAAATGATGTTCTATATGTAGCCAATCTAGGGGACTCGAGAGCTGTCCTTGGCCGGAGAGTTTCGGATAGTAAGAGGAGTCCGGTGGTGGCAGAACGCTTGTCCACTGATCACAATGTGGGAGTTGAGGAGGTCAGGAAGGAGGTTGAAGCACTACATCCCGATGATTCACATGCTGTGGTGTATATTCATGGAGTTTGGAGGATAAAAGGCATAATTCAGGTGTCAAGATCTATTGGTGATGTTTATTTAAAGAAGCCGGAGTTTAACAGAGACCCCATATACCAGCAATTTGCGAACCCTGTTCCTATGAAACGGCCAGTGATGACTGCAGAGCCCTCAATCATTACAAGGAACCTTAAGCCACAGGATCTGTTTTTGATCTTTGCCTCAGATGGCCTATGGGAACAGTTGAGCGATGATGCAGCAGTAGAAATTGTTTTCAAAAACCCAAGAGCTGGTATTGCAAAGAGATTAGTAAGAGCTGCTCTTGAAGAAGCTGCAAAGAAGAGGGAGATGAGATACAATGACATAACGAAAATCAAAAAGGGAATAAGGCGGCACTTTCATGATGACATCACTGTTATAGTAGTCTATCTTGATGACCACAAAAGCTCCTCGAAATGTAAATCTAAGAATATCATTGTTGGCTCTACCAGAGCACCTGCTGATATTTTCTCGGTCAGTGCAGATAAAGCGGAGCAGGATCTGCATCACATGATCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

383

Amino Acids

42.37

Weight (kDa)

8.6

Isoelectric Point (pI)

48.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	60 - 310	1.4e-37	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001678)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33920 AT4G33920 AT4G33920
fragaria_vesca	FvH4_1g17120 FvH4_1g17120 FvH4_2g04560 FvH4_2g04560 FvH4_2g04560 FvH4_2g04560
malus_domestica	MD02G1181600.v1.1 MD05G1095000.v1.1 MD07G1193800.v1.1 MD09G1197700.v1.1 MD10G1102000.v1.1 MD15G1292500.v1.1
prunus_persica	Prupe.6G224900_v2.0.a1 Prupe.8G139700_v2.0.a1
pyrus_communis	pycom02g14740 pycom05g09390 pycom10g08770 pycom15g25550
rosa_chinensis	RchiOBHm_Chr2g0107291 RchiOBHm_Chr6g0254141
rosa_laevigata	RLG00000003413 RLG00000014940 RLG00000017568
rosa_multiflora	Rmu_co8069786.1_g000001 Rmu_sc0000010.1_g000028 Rmu_sc0003711.1_g000001 Rmu_sc0006416.1_g000004
rosa_roxburghii	Rroxscaffold_2G00136460 Rroxscaffold_5G00349630 Rroxscaffold_7G00211130
rosa_rugosa	Rorug02G0036100 Rorug02G0141400 Rorug02G0141500 Rorug02G0381600 Rorug04G0161000 Rorug05G0094200 Rorug05G0550000 Rorug07G0100900
rosa_samantha	Rh2CG197700 Rh6AG066500 Rh6BG059600 Rh6CG059300 Rh6CG524200 Rh6DG056200
rosa_wichuraiana	Rw2G015230 Rw6G005840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1093
Acc36I	ACCTGC	1 cut(s)	1093
AccB7I	CCANNNNNTGG	1 cut(s)	878
AciI	CCGC	4 cut(s)	63, 216, 979, 1118
AclWI	GGATC	3 cut(s)	162, 798, 1134
AcoI	YGGCCR	3 cut(s)	208, 472, 737
AcsI	RAATTY	2 cut(s)	145, 269
AcuI	CTGAAG	1 cut(s)	351
AdeI	CACNNNGTG	1 cut(s)	538
AfeI	AGCGCT	1 cut(s)	32
AfiI	CCNNNNNNNGG	2 cut(s)	450, 878
AflII	CTTAAG	1 cut(s)	779
AgsI	TTSAA	3 cut(s)	569, 865, 914
AhdI	GACNNNNNGTC	1 cut(s)	94
AluBI	AGCT	6 cut(s)	227, 464, 878, 905, 920, 1035
AluI	AGCT	6 cut(s)	227, 464, 878, 905, 920, 1035
Alw21I	GWGCWC	1 cut(s)	1084
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	3 cut(s)	162, 798, 1134
AlwNI	CAGNNNCTG	2 cut(s)	793, 1129
Ama87I	CYCGRG	2 cut(s)	26, 457
Aor51HI	AGCGCT	1 cut(s)	32
AoxI	GGCC	6 cut(s)	73, 135, 208, 472, 737, 817
ApeKI	GCWGC	5 cut(s)	4, 22, 845, 905, 920
ApoI	RAATTY	2 cut(s)	145, 269
Asp700I	GAANNNNTTC	1 cut(s)	317
AspLEI	GCGC	2 cut(s)	9, 33
AspS9I	GGNCC	1 cut(s)	218
AsuHPI	GGTGA	4 cut(s)	122, 163, 364, 671
AsuII	TTCGAA	1 cut(s)	143
AvaI	CYCGRG	2 cut(s)	26, 457
AvaII	GGWCC	1 cut(s)	218
BalI	TGGCCA	1 cut(s)	210
BanII	GRGCYC	1 cut(s)	759
BauI	CACGAG	1 cut(s)	10
BbsI	GAAGAC	1 cut(s)	160
Bbv12I	GWGCWC	1 cut(s)	1084
BbvI	GCAGC	4 cut(s)	9, 857, 892, 907
BccI	CCATC	5 cut(s)	65, 200, 255, 271, 809
BceAI	ACGGC	1 cut(s)	752
BclI	TGATCA	1 cut(s)	529
BcoDI	GTCTC	1 cut(s)	687
BfaI	CTAG	2 cut(s)	231, 449
BfmI	CTRYAG	2 cut(s)	16, 750
BfoI	RGCGCY	1 cut(s)	34
BfrI	CTTAAG	1 cut(s)	779
BfuAI	ACCTGC	1 cut(s)	1093
BglII	AGATCT	1 cut(s)	650
BisI	GCNGC	6 cut(s)	5, 23, 846, 906, 921, 980
BlsI	GCNGC	6 cut(s)	6, 24, 847, 907, 922, 981
Bme18I	GGWCC	1 cut(s)	218
BmeRI	GACNNNNNGTC	1 cut(s)	94
BmeT110I	CYCGRG	2 cut(s)	26, 457
BmgT120I	GGNCC	1 cut(s)	218
BmiI	GGNNCC	2 cut(s)	220, 776
BmsI	GCATC	3 cut(s)	363, 832, 1141
BpiI	GAAGAC	1 cut(s)	160
Bpu14I	TTCGAA	1 cut(s)	143
BsaBI	GATNNNNATC	1 cut(s)	1131
BsaI	GGTCTC	1 cut(s)	687
BsaJI	CCNNGG	2 cut(s)	211, 469
BsaWI	WCCGGW	2 cut(s)	107, 502
BsaXI	ACNNNNNCTCC	2 cut(s)	1019, 1049
Bsc4I	CCNNNNNNNGG	2 cut(s)	450, 878
Bse1I	ACTGG	1 cut(s)	740
Bse8I	GATNNNNATC	1 cut(s)	1131
BseDI	CCNNGG	2 cut(s)	211, 469
BseGI	GGATG	1 cut(s)	577
BseJI	GATNNNNATC	1 cut(s)	1131
BseLI	CCNNNNNNNGG	2 cut(s)	450, 878
BseMII	CTCAG	1 cut(s)	825
BseNI	ACTGG	1 cut(s)	740
BseRI	GAGGAG	4 cut(s)	242, 511, 563, 1027
BseXI	GCAGC	4 cut(s)	9, 857, 892, 907
BseYI	CCCAGC	1 cut(s)	56
BsgI	GTGCAG	1 cut(s)	1128
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	6 cut(s)	75, 137, 210, 474, 739, 819
BsiEI	CGRYCG	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	1084
BsiHKCI	CYCGRG	2 cut(s)	26, 457
BsiSI	CCGG	4 cut(s)	108, 475, 503, 680
BslFI	GGGAC	3 cut(s)	82, 204, 467
BslI	CCNNNNNNNGG	2 cut(s)	450, 878
BsmAI	GTCTC	1 cut(s)	687
BsmFI	GGGAC	3 cut(s)	82, 204, 467
BsmI	GAATGC	1 cut(s)	317
BsnI	GGCC	6 cut(s)	75, 137, 210, 474, 739, 819
Bso31I	GGTCTC	1 cut(s)	687
BsoBI	CYCGRG	2 cut(s)	26, 457
Bsp119I	TTCGAA	1 cut(s)	143
Bsp1286I	GDGCHC	2 cut(s)	759, 1084
Bsp143I	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
Bsp19I	CCATGG	1 cut(s)	211
BspACI	CCGC	4 cut(s)	63, 216, 979, 1118
BspANI	GGCC	6 cut(s)	75, 137, 210, 474, 739, 819
BspCNI	CTCAG	1 cut(s)	824
BspHI	TCATGA	2 cut(s)	988, 1145
BspLI	GGNNCC	2 cut(s)	220, 776
BspMAI	CTGCAG	1 cut(s)	754
BspMI	ACCTGC	1 cut(s)	1093
BspPI	GGATC	3 cut(s)	162, 798, 1134
BspT104I	TTCGAA	1 cut(s)	143
BspTI	CTTAAG	1 cut(s)	779
BspTNI	GGTCTC	1 cut(s)	687
BsrI	ACTGG	1 cut(s)	740
BssECI	CCNNGG	2 cut(s)	211, 469
BssMI	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
BssSI	CACGAG	1 cut(s)	10
BssT1I	CCWWGG	2 cut(s)	211, 469
Bst2BI	CACGAG	1 cut(s)	10
Bst4CI	ACNGT	2 cut(s)	831, 1003
Bst6I	CTCTTC	1 cut(s)	923
BstAFI	CTTAAG	1 cut(s)	779
BstAPI	GCANNNNNTGC	2 cut(s)	323, 1129
BstBI	TTCGAA	1 cut(s)	143
BstDEI	CTNAG	2 cut(s)	811, 1053
BstDSI	CCRYGG	1 cut(s)	211
BstF5I	GGATG	1 cut(s)	577
BstH2I	RGCGCY	1 cut(s)	34
BstHHI	GCGC	2 cut(s)	9, 33
BstKTI	GATC	9 cut(s)	123, 157, 362, 532, 653, 793, 804, 1129, 1143
BstMAI	GTCTC	1 cut(s)	687
BstMBI	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
BstMCI	CGRYCG	1 cut(s)	123
BstMWI	GCNNNNNNNGC	7 cut(s)	28, 323, 364, 373, 884, 1115, 1129
BstNSI	RCATGY	1 cut(s)	596
BstSFI	CTRYAG	2 cut(s)	16, 750
BstV1I	GCAGC	4 cut(s)	9, 857, 892, 907
BstV2I	GAAGAC	1 cut(s)	160
BstX2I	RGATCY	3 cut(s)	650, 790, 1126
BstYI	RGATCY	3 cut(s)	650, 790, 1126
BsuRI	GGCC	6 cut(s)	75, 137, 210, 474, 739, 819
BtgI	CCRYGG	1 cut(s)	211
BtgZI	GCGATG	4 cut(s)	61, 84, 290, 852
BtsCI	GGATG	1 cut(s)	577
BtsIMutI	CAGTG	6 cut(s)	327, 328, 525, 747, 999, 1111
BveI	ACCTGC	1 cut(s)	1093
CaiI	CAGNNNCTG	2 cut(s)	793, 1129
CciI	TCATGA	2 cut(s)	988, 1145
CfoI	GCGC	2 cut(s)	9, 33
Cfr13I	GGNCC	1 cut(s)	218
CviAII	CATG	7 cut(s)	85, 212, 593, 611, 989, 1138, 1146
DdeI	CTNAG	2 cut(s)	811, 1053
DpnI	GATC	9 cut(s)	122, 156, 361, 531, 652, 792, 803, 1128, 1142
DpnII	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
DraI	TTTAAA	1 cut(s)	672
DraIII	CACNNNGTG	1 cut(s)	538
DriI	GACNNNNNGTC	1 cut(s)	94
EaeI	YGGCCR	3 cut(s)	208, 472, 737
Eam1104I	CTCTTC	1 cut(s)	923
Eam1105I	GACNNNNNGTC	1 cut(s)	94
EarI	CTCTTC	1 cut(s)	923
EciI	GGCGGA	1 cut(s)	78
Eco130I	CCWWGG	2 cut(s)	211, 469
Eco24I	GRGCYC	1 cut(s)	759
Eco31I	GGTCTC	1 cut(s)	687
Eco47I	GGWCC	1 cut(s)	218
Eco47III	AGCGCT	1 cut(s)	32
Eco57I	CTGAAG	1 cut(s)	351
Eco88I	CYCGRG	2 cut(s)	26, 457
EcoT14I	CCWWGG	2 cut(s)	211, 469
EcoT38I	GRGCYC	1 cut(s)	759
ErhI	CCWWGG	2 cut(s)	211, 469
FaeI	CATG	7 cut(s)	88, 215, 596, 614, 992, 1141, 1149
FaqI	GGGAC	3 cut(s)	82, 204, 467
FatI	CATG	7 cut(s)	84, 211, 592, 610, 988, 1137, 1145
FbaI	TGATCA	1 cut(s)	529
Fnu4HI	GCNGC	6 cut(s)	5, 23, 846, 906, 921, 980
FokI	GGATG	1 cut(s)	564
FriOI	GRGCYC	1 cut(s)	759
Fsp4HI	GCNGC	6 cut(s)	5, 23, 846, 906, 921, 980
FspBI	CTAG	2 cut(s)	231, 449
GlaI	GCGC	2 cut(s)	8, 32
GluI	GCNGC	6 cut(s)	5, 23, 846, 906, 921, 980
GsaI	CCCAGC	1 cut(s)	60
HaeII	RGCGCY	1 cut(s)	34
HaeIII	GGCC	6 cut(s)	75, 137, 210, 474, 739, 819
HapII	CCGG	4 cut(s)	108, 475, 503, 680
HhaI	GCGC	2 cut(s)	9, 33
Hin1II	CATG	7 cut(s)	88, 215, 596, 614, 992, 1141, 1149
Hin6I	GCGC	2 cut(s)	7, 31
HinP1I	GCGC	2 cut(s)	7, 31
HincII	GTYRAC	1 cut(s)	241
HindII	GTYRAC	1 cut(s)	241
HindIII	AAGCTT	1 cut(s)	225
HinfI	GANTC	5 cut(s)	95, 234, 455, 499, 587
HpaII	CCGG	4 cut(s)	108, 475, 503, 680
HphI	GGTGA	4 cut(s)	122, 163, 364, 671
Hpy166II	GTNNAC	2 cut(s)	241, 525
Hpy188I	TCNGA	2 cut(s)	487, 814
Hpy188III	TCNNGA	9 cut(s)	231, 459, 556, 581, 648, 911, 989, 1019, 1146
Hpy8I	GTNNAC	2 cut(s)	241, 525
HpyAV	CCTTC	2 cut(s)	307, 553
HpyCH4III	ACNGT	2 cut(s)	831, 1003
HpyCH4IV	ACGT	1 cut(s)	188
HpyCH4V	TGCA	8 cut(s)	185, 346, 752, 845, 887, 923, 1109, 1132
HpyF10VI	GCNNNNNNNGC	7 cut(s)	28, 323, 364, 373, 884, 1115, 1129
HpyF3I	CTNAG	2 cut(s)	811, 1053
HpySE526I	ACGT	1 cut(s)	188
Hsp92II	CATG	7 cut(s)	88, 215, 596, 614, 992, 1141, 1149
HspAI	GCGC	2 cut(s)	7, 31
Ksp22I	TGATCA	1 cut(s)	529
Kzo9I	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
LmnI	GCTCC	3 cut(s)	413, 1040, 1120
Lsp1109I	GCAGC	4 cut(s)	9, 857, 892, 907
LweI	GCATC	3 cut(s)	363, 832, 1141
MaeI	CTAG	2 cut(s)	231, 449
MaeII	ACGT	1 cut(s)	188
MaeIII	GTNAC	1 cut(s)	110
MalI	GATC	9 cut(s)	122, 156, 361, 531, 652, 792, 803, 1128, 1142
MboI	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
MboII	GAAGA	5 cut(s)	160, 344, 347, 926, 940
MflI	RGATCY	3 cut(s)	650, 790, 1126
MhlI	GDGCHC	2 cut(s)	759, 1084
MlsI	TGGCCA	1 cut(s)	210
MluCI	AATT	6 cut(s)	139, 145, 269, 636, 710, 855
MluNI	TGGCCA	1 cut(s)	210
MlyI	GAGTC	3 cut(s)	104, 449, 508
Mox20I	TGGCCA	1 cut(s)	210
MroXI	GAANNNNTTC	1 cut(s)	317
MscI	TGGCCA	1 cut(s)	210
MseI	TTAA	3 cut(s)	671, 687, 780
MslI	CAYNNNNRTG	2 cut(s)	582, 987
Msp20I	TGGCCA	1 cut(s)	210
MspCI	CTTAAG	1 cut(s)	779
MspI	CCGG	4 cut(s)	108, 475, 503, 680
Mva1269I	GAATGC	1 cut(s)	317
MwoI	GCNNNNNNNGC	7 cut(s)	28, 323, 364, 373, 884, 1115, 1129
NcoI	CCATGG	1 cut(s)	211
NdeII	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
NlaIII	CATG	7 cut(s)	88, 215, 596, 614, 992, 1141, 1149
NlaIV	GGNNCC	2 cut(s)	220, 776
NmuCI	GTSAC	1 cut(s)	110
NspI	RCATGY	1 cut(s)	596
NspV	TTCGAA	1 cut(s)	143
PaeR7I	CTCGAG	2 cut(s)	26, 457
PagI	TCATGA	2 cut(s)	988, 1145
PaqCI	CACCTGC	1 cut(s)	1093
PctI	GAATGC	1 cut(s)	317
PdmI	GAANNNNTTC	1 cut(s)	317
PfeI	GAWTC	2 cut(s)	234, 587
PflMI	CCANNNNNTGG	1 cut(s)	878
PkrI	GCNGC	6 cut(s)	6, 24, 847, 907, 922, 981
Ple19I	CGATCG	1 cut(s)	123
PleI	GAGTC	3 cut(s)	103, 449, 507
PpsI	GAGTC	3 cut(s)	103, 449, 507
PspFI	CCCAGC	1 cut(s)	56
PspN4I	GGNNCC	2 cut(s)	220, 776
PspPI	GGNCC	1 cut(s)	218
PspXI	VCTCGAGB	1 cut(s)	26
PstI	CTGCAG	1 cut(s)	754
PstNI	CAGNNNCTG	2 cut(s)	793, 1129
PsuI	RGATCY	3 cut(s)	650, 790, 1126
PvuI	CGATCG	1 cut(s)	123
RseI	CAYNNNNRTG	2 cut(s)	582, 987
SaqAI	TTAA	3 cut(s)	671, 687, 780
SatI	GCNGC	6 cut(s)	5, 23, 846, 906, 921, 980
Sau3AI	GATC	9 cut(s)	120, 154, 359, 529, 650, 790, 801, 1126, 1140
Sau96I	GGNCC	1 cut(s)	218
SchI	GAGTC	3 cut(s)	104, 449, 508
SduI	GDGCHC	2 cut(s)	759, 1084
SfaNI	GCATC	3 cut(s)	363, 832, 1141
SfcI	CTRYAG	2 cut(s)	16, 750
Sfr274I	CTCGAG	2 cut(s)	26, 457
SfuI	TTCGAA	1 cut(s)	143
SinI	GGWCC	1 cut(s)	218
SlaI	CTCGAG	2 cut(s)	26, 457
SmiMI	CAYNNNNRTG	2 cut(s)	582, 987
SmlI	CTYRAG	3 cut(s)	26, 457, 779
SmoI	CTYRAG	3 cut(s)	26, 457, 779
Sse9I	AATT	6 cut(s)	139, 145, 269, 636, 710, 855
SsiI	CCGC	4 cut(s)	63, 216, 979, 1118
SspMI	CTAG	2 cut(s)	231, 449
StyI	CCWWGG	2 cut(s)	211, 469
TaaI	ACNGT	2 cut(s)	831, 1003
TaiI	ACGT	1 cut(s)	191
TaqI	TCGA	5 cut(s)	27, 119, 143, 458, 1040
TaqII	GACCGA	1 cut(s)	1090
TasI	AATT	6 cut(s)	139, 145, 269, 636, 710, 855
TauI	GCSGC	1 cut(s)	982
TfiI	GAWTC	2 cut(s)	234, 587
Tru1I	TTAA	3 cut(s)	671, 687, 780
Tru9I	TTAA	3 cut(s)	671, 687, 780
TscAI	CASTG	6 cut(s)	328, 334, 532, 747, 1006, 1111
TseFI	GTSAC	1 cut(s)	110
TseI	GCWGC	5 cut(s)	4, 22, 845, 905, 920
Tsp45I	GTSAC	1 cut(s)	110
TspDTI	ATGAA	3 cut(s)	599, 746, 977
TspGWI	ACGGA	1 cut(s)	379
TspRI	CASTG	6 cut(s)	328, 334, 532, 747, 1006, 1111
Van91I	CCANNNNNTGG	1 cut(s)	878
Vha464I	CTTAAG	1 cut(s)	779
VpaK11BI	GGWCC	1 cut(s)	218
XapI	RAATTY	2 cut(s)	145, 269
XbaI	TCTAGA	1 cut(s)	230
XceI	RCATGY	1 cut(s)	596
XcmI	CCANNNNNNNNNTGG	1 cut(s)	145
XhoI	CTCGAG	2 cut(s)	26, 457
XmnI	GAANNNNTTC	1 cut(s)	317
XspI	CTAG	2 cut(s)	231, 449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.