MD07G1193800.v1.1

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001678
Physical Location & Seq
Forward (+)
27350576 .. 27352543
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1193800.v1.1.491

Sequence Viewer

Length: 534 bp
ATGATGTTGGAGATGTGTAGGAGGCCGTTGGAGAAGTGTTTTGGTGGCGGAGACGGCGGCGATGGCCTGCTTTGGCACATGGACCTCAAGCCACACTCTTCTGGGAACTATTCGATTGCAGTGGTTCAAGCAAATTCGGCGCTGGAAGATCAGGGACAGGTGCATTTTGATGCAACAGAGGAGGAGTTCTTGGAGTTGGTTAAGCGATCATGGATGGTGCGGCCGCAGATTGCTTCAGTAGGATCGTGTTGCCTGGTTGGTGCAATTGCGGATGGTCTTTTATATGTGGCTAATCTCGGAGATTCGAGGGCAGTTCTTGGCAGGAGATCATCGGATGGGCAGGCGGTGGTGGCAGAGCGGTTGTCTACGGATCACAATGTTGCAGTGGAGGAAGTGAGGAAGGAGGTTAAGGATCTTCACCCTGATGATGCGCACATTGTGGTGTTTACGCGTGGAGTTTGGCGAATTAAGGGCATTATTCAGGGTTGTTCGAATAACTTAGTTCAAATGGTAAGTCTCAAGGTCAATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

178

Amino Acids

19.4

Weight (kDa)

5.85

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 52 - 130 8.9e-09 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 365
AccII CGCG 1 cut(s) 451
AciI CCGC 7 cut(s) 48, 57, 220, 224, 269, 344, 358
AclWI GGATC 3 cut(s) 250, 378, 420
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 439
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 2 cut(s) 128, 506
AjnI CCWGG 1 cut(s) 252
Alw26I GTCTC 2 cut(s) 45, 521
AlwI GGATC 3 cut(s) 250, 378, 420
AoxI GGCC 3 cut(s) 23, 64, 221
ApoI RAATTY 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AspLEI GCGC 2 cut(s) 142, 433
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 491
AvaII GGWCC 1 cut(s) 82
BccI CCATC 4 cut(s) 56, 208, 266, 329
BceAI ACGGC 2 cut(s) 10, 70
BciT130I CCWGG 1 cut(s) 254
BcoDI GTCTC 2 cut(s) 45, 521
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 3 cut(s) 58, 221, 224
BlsI GCNGC 3 cut(s) 59, 222, 225
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 160, 418
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 2 cut(s) 71, 503
BseBI CCWGG 1 cut(s) 254
BseGI GGATG 3 cut(s) 219, 277, 340
BseRI GAGGAG 2 cut(s) 194, 197
BseX3I CGGCCG 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 451
Bsh1285I CGRYCG 1 cut(s) 224
BshFI GGCC 3 cut(s) 25, 66, 223
BsiEI CGRYCG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 168
BsmAI GTCTC 2 cut(s) 45, 521
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 3 cut(s) 25, 66, 223
Bsp119I TTCGAA 1 cut(s) 491
Bsp143I GATC 6 cut(s) 148, 206, 242, 326, 370, 412
BspACI CCGC 7 cut(s) 48, 57, 220, 224, 269, 344, 358
BspANI GGCC 3 cut(s) 25, 66, 223
BspFNI CGCG 1 cut(s) 451
BspPI GGATC 3 cut(s) 250, 378, 420
BspT104I TTCGAA 1 cut(s) 491
BsrBI CCGCTC 1 cut(s) 358
BssMI GATC 6 cut(s) 148, 206, 242, 326, 370, 412
Bst2UI CCWGG 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 68, 342
BstDEI CTNAG 1 cut(s) 499
BstF5I GGATG 3 cut(s) 219, 277, 340
BstFNI CGCG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 2 cut(s) 142, 433
BstKTI GATC 6 cut(s) 151, 209, 245, 329, 373, 415
BstMAI GTCTC 2 cut(s) 45, 521
BstMBI GATC 6 cut(s) 148, 206, 242, 326, 370, 412
BstMCI CGRYCG 1 cut(s) 224
BstMWI GCNNNNNNNGC 4 cut(s) 54, 63, 137, 350
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 252
BstUI CGCG 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BstZI CGGCCG 1 cut(s) 221
BsuRI GGCC 3 cut(s) 25, 66, 223
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 219, 277, 340
BtsI GCAGTG 2 cut(s) 126, 390
BtsIMutI CAGTG 2 cut(s) 126, 390
Cac8I GCNNGC 2 cut(s) 68, 342
CciNI GCGGCCGC 1 cut(s) 221
CfoI GCGC 2 cut(s) 142, 433
Cfr13I GGNCC 1 cut(s) 82
CviAII CATG 2 cut(s) 79, 210
CviJI RGCY 5 cut(s) 25, 66, 91, 223, 290
CviKI_1 RGCY 5 cut(s) 25, 66, 91, 223, 290
DdeI CTNAG 1 cut(s) 499
DpnI GATC 6 cut(s) 150, 208, 244, 328, 372, 414
DpnII GATC 6 cut(s) 148, 206, 242, 326, 370, 412
DraIII CACNNNGTG 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 221
EagI CGGCCG 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
EciI GGCGGA 1 cut(s) 63
EclXI CGGCCG 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 2 cut(s) 82, 213
FaiI YATR 4 cut(s) 80, 211, 283, 285
FaqI GGGAC 1 cut(s) 168
FatI CATG 2 cut(s) 78, 209
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 3 cut(s) 58, 221, 224
FokI GGATG 3 cut(s) 226, 284, 347
Fsp4HI GCNGC 3 cut(s) 58, 221, 224
FspAI RTGCGCAY 1 cut(s) 432
FspI TGCGCA 1 cut(s) 432
GlaI GCGC 2 cut(s) 141, 432
GluI GCNGC 3 cut(s) 58, 221, 224
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 3 cut(s) 25, 66, 223
HhaI GCGC 2 cut(s) 142, 433
Hin1II CATG 2 cut(s) 82, 213
Hin6I GCGC 2 cut(s) 140, 431
HinP1I GCGC 2 cut(s) 140, 431
HinfI GANTC 1 cut(s) 302
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 366, 447
Hpy188I TCNGA 2 cut(s) 299, 334
Hpy8I GTNNAC 2 cut(s) 366, 447
HpyAV CCTTC 1 cut(s) 394
HpyCH4V TGCA 5 cut(s) 119, 163, 173, 263, 383
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 63, 137, 350
HpyF3I CTNAG 1 cut(s) 499
Hsp92II CATG 2 cut(s) 82, 213
HspAI GCGC 2 cut(s) 140, 431
Kzo9I GATC 6 cut(s) 148, 206, 242, 326, 370, 412
LweI GCATC 2 cut(s) 160, 418
MalI GATC 6 cut(s) 150, 208, 244, 328, 372, 414
MbiI CCGCTC 1 cut(s) 358
MboI GATC 6 cut(s) 148, 206, 242, 326, 370, 412
MboII GAAGA 3 cut(s) 90, 158, 407
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 412
MluCI AATT 3 cut(s) 133, 264, 465
MluI ACGCGT 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 8 cut(s) 15, 95, 172, 175, 300, 382, 390, 397
MseI TTAA 3 cut(s) 201, 408, 468
MslI CAYNNNNRTG 3 cut(s) 168, 423, 440
MspR9I CCNGG 1 cut(s) 254
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 4 cut(s) 54, 63, 137, 350
NdeII GATC 6 cut(s) 148, 206, 242, 326, 370, 412
NlaIII CATG 2 cut(s) 82, 213
NotI GCGGCCGC 1 cut(s) 221
NsbI TGCGCA 1 cut(s) 432
NspV TTCGAA 1 cut(s) 491
PfeI GAWTC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 59, 222, 225
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspPI GGNCC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 412
RseI CAYNNNNRTG 3 cut(s) 168, 423, 440
SaqAI TTAA 3 cut(s) 201, 408, 468
SatI GCNGC 3 cut(s) 58, 221, 224
Sau3AI GATC 6 cut(s) 148, 206, 242, 326, 370, 412
Sau96I GGNCC 1 cut(s) 82
ScrFI CCNGG 1 cut(s) 254
SetI ASST 4 cut(s) 87, 162, 408, 525
SfaNI GCATC 2 cut(s) 160, 418
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 3 cut(s) 168, 423, 440
SmlI CTYRAG 2 cut(s) 86, 518
SmoI CTYRAG 2 cut(s) 86, 518
Sse9I AATT 3 cut(s) 133, 264, 465
SsiI CCGC 7 cut(s) 48, 57, 220, 224, 269, 344, 358
StyD4I CCNGG 1 cut(s) 252
TaqI TCGA 3 cut(s) 113, 305, 491
TasI AATT 3 cut(s) 133, 264, 465
TauI GCSGC 3 cut(s) 60, 223, 226
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 3 cut(s) 201, 408, 468
Tru9I TTAA 3 cut(s) 201, 408, 468
TscAI CASTG 2 cut(s) 126, 390
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 2 cut(s) 126, 390
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.