Rroxscaffold_7G00211130

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001678
Physical Location & Seq
Reverse (-)
61572105 .. 61574510
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211130.1

Sequence Viewer

Length: 1323 bp
ATGCTGCGGACGATTTCGAGGCCGTTAGAGCGGTGTTTCGGGTTGAGACTTGGGTCCGGCGGCGGTGATGGGTTGCTTTGGCATACCGACTTGAAGCCTCACGCCTCCGGTGACTACTCGATTGCTGTGGTTCAGGCAAATTCGAGCCTTGAGGATCAGGGACAGGTGTTTACCTCTCCCTCTGCCACGTACGTCGGCGTGTATGATGGTCACGGCGGACCGGAAGCTTCTAGATTCGTCAACAATAATCTGTTCCCGTTTTTACACAAATTTGCCATGGAACAAGGTGGGTTATCTGCGGATGTGATAAAGAAGGCGTTCAGTGCCACTGAGGAGGAGTTTCTGCGTTTGGTGAAGCGATCGTGGGCAATGAAGCCGTCAATTGCTTCTGTCGGGTCATGTTGTTTGGTCGGGGCTATAACAAATGATGTTCTGTATGTTGCGAATCTGGGGGATTCGAGAGCAGTCCTTGGCCGGAAAGTGAACGGGAGTAAGAGGACGGTGGTGGCAGAACGGTTGTCAACGGATCACAATGTCAGTGATGAGCAGGTCAGGAGGGAAGTTGAAGCACTTCATCCTGACGATTCACATGTTGTGGTGTATACTCGCGGAGTTTGGAGGATAAAGGGGATAATTCAGTTCAAAGCACCTAAACTATCTCATGTCAATTTGCAACATCAGTTCAATATTCAAATTCTTAGTGAACATTCTAAGGGGCCATTGCCTGGTGCAATGGTCAAATTTGGGCTTCTGAGTATGCAAGAATATTATCCTCTGCCTCCTGGTCCAGCATCTCTTGCAGGTAAGTTAGAGGTGTCAAGATCTATTGGGGATGTTTATCTAAAGAAGCCTGAGTTTAACAGAGACCCAATGTACCAGCAGCTTATTACCCCTGTTCCTATGAAGCGGCCAGTGGTGACAGCAGAGCCCTCCATCCTAATTAGAAACATTAAGCCACAGGATATGTTTTTGATATTTGCATCAGATGGCCTATGGGAACAGTTGAGTGATGAGGCAGCAGTAGAGCTAGTCTTCAAGAACCCTAGAGCTGGTATTGCCAAGAGACTGGTAAGGGCTGCTCTCCATGAAGCCGCCAAGAAGAGGGAAATGAGATATGATGACATAAAGAAAATTGAAAAGGGAATAAGGCGGCATTTTCATGATGATATTACTGTCATTGTAGTCTATCTTGATCAGCACAAAAGCTCCTCAAAACATAAACTTGGCAACAATGCTCTAAGTTCCATCACCCAAGCACCTGTTGACATTTACTCGCTTAGTGCAGAGGAAGTAGAACAGGATATGCATCATATCATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

440

Amino Acids

48.92

Weight (kDa)

8.98

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 196 4.2e-17 Protein phosphatase 2C
PP2C PF00481 266 - 366 4.7e-17 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 538, 791
AccB7I CCANNNNNTGG 1 cut(s) 725
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 602
AccII CGCG 1 cut(s) 609
AclWI GGATC 2 cut(s) 162, 534
AcoI YGGCCR 2 cut(s) 472, 908
AcsI RAATTY 4 cut(s) 139, 269, 693, 740
AfaI GTAC 2 cut(s) 191, 875
AfiI CCNNNNNNNGG 3 cut(s) 725, 1049, 1101
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 7 cut(s) 94, 566, 643, 685, 692, 1036, 1136
AjnI CCWGG 2 cut(s) 724, 781
AluBI AGCT 5 cut(s) 227, 883, 1027, 1049, 1206
AluI AGCT 5 cut(s) 227, 883, 1027, 1049, 1206
Alw26I GTCTC 3 cut(s) 40, 858, 1057
AlwI GGATC 2 cut(s) 162, 534
AoxI GGCC 5 cut(s) 20, 472, 716, 908, 988
ApeKI GCWGC 4 cut(s) 4, 880, 1016, 1076
ApoI RAATTY 4 cut(s) 139, 269, 693, 740
Asp700I GAANNNNTTC 2 cut(s) 317, 570
AspS9I GGNCC 4 cut(s) 54, 218, 716, 785
AsuHPI GGTGA 5 cut(s) 77, 122, 364, 928, 1240
AvaII GGWCC 3 cut(s) 54, 218, 785
BanII GRGCYC 1 cut(s) 930
BbsI GAAGAC 1 cut(s) 1024
BbvI GCAGC 3 cut(s) 892, 1028, 1063
BccI CCATC 5 cut(s) 62, 200, 941, 980, 1253
BceAI ACGGC 3 cut(s) 7, 229, 361
BciT130I CCWGG 2 cut(s) 726, 783
BclI TGATCA 1 cut(s) 1192
BcoDI GTCTC 3 cut(s) 40, 858, 1057
BfaI CTAG 3 cut(s) 231, 1028, 1044
BfuAI ACCTGC 2 cut(s) 538, 791
BglII AGATCT 1 cut(s) 821
BisI GCNGC 8 cut(s) 5, 61, 881, 908, 1017, 1077, 1092, 1151
BlsI GCNGC 8 cut(s) 6, 62, 882, 909, 1018, 1078, 1093, 1152
Bme1390I CCNGG 2 cut(s) 726, 783
Bme18I GGWCC 3 cut(s) 54, 218, 785
BmgT120I GGNCC 4 cut(s) 54, 218, 716, 785
BmiI GGNNCC 2 cut(s) 55, 717
BmrFI CCNGG 2 cut(s) 726, 783
BmsI GCATC 3 cut(s) 800, 989, 1315
BoxI GACNNNNGTC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 1024
BpuEI CTTGAG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 189
BsaBI GATNNNNATC 2 cut(s) 837, 1305
BsaI GGTCTC 1 cut(s) 858
BsaJI CCNNGG 2 cut(s) 276, 469
BsaWI WCCGGW 2 cut(s) 107, 220
BsaXI ACNNNNNCTCC 2 cut(s) 1190, 1220
Bsc4I CCNNNNNNNGG 3 cut(s) 725, 1049, 1101
Bse1I ACTGG 2 cut(s) 911, 1071
Bse3DI GCAATG 3 cut(s) 375, 719, 738
Bse8I GATNNNNATC 2 cut(s) 837, 1305
BseBI CCWGG 2 cut(s) 726, 783
BseDI CCNNGG 2 cut(s) 276, 469
BseGI GGATG 4 cut(s) 307, 574, 838, 933
BseJI GATNNNNATC 2 cut(s) 837, 1305
BseLI CCNNNNNNNGG 3 cut(s) 725, 1049, 1101
BseMI GCAATG 3 cut(s) 375, 719, 738
BseMII CTCAG 3 cut(s) 321, 743, 843
BseNI ACTGG 2 cut(s) 911, 1071
BseRI GAGGAG 3 cut(s) 347, 350, 1198
BseXI GCAGC 3 cut(s) 892, 1028, 1063
BsgI GTGCAG 1 cut(s) 1302
Bsh1236I CGCG 1 cut(s) 609
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 5 cut(s) 22, 474, 718, 910, 990
BsiEI CGRYCG 1 cut(s) 362
BsiSI CCGG 4 cut(s) 57, 108, 221, 475
BsiWI CGTACG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 3 cut(s) 725, 1049, 1101
BsmAI GTCTC 3 cut(s) 40, 858, 1057
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 5 cut(s) 22, 474, 718, 910, 990
Bso31I GGTCTC 1 cut(s) 858
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 5 cut(s) 154, 359, 526, 821, 1192
Bsp19I CCATGG 1 cut(s) 276
BspANI GGCC 5 cut(s) 22, 474, 718, 910, 990
BspCNI CTCAG 3 cut(s) 322, 744, 844
BspFNI CGCG 1 cut(s) 609
BspHI TCATGA 1 cut(s) 1159
BspLI GGNNCC 2 cut(s) 55, 717
BspMI ACCTGC 2 cut(s) 538, 791
BspPI GGATC 2 cut(s) 162, 534
BspTNI GGTCTC 1 cut(s) 858
BsrBI CCGCTC 1 cut(s) 31
BsrDI GCAATG 3 cut(s) 375, 719, 738
BsrI ACTGG 2 cut(s) 911, 1071
BssECI CCNNGG 2 cut(s) 276, 469
BssMI GATC 5 cut(s) 154, 359, 526, 821, 1192
BssNAI GTATAC 1 cut(s) 603
BssT1I CCWWGG 2 cut(s) 276, 469
Bst1107I GTATAC 1 cut(s) 603
Bst2UI CCWGG 2 cut(s) 726, 783
Bst4CI ACNGT 4 cut(s) 502, 516, 1002, 1174
Bst6I CTCTTC 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 797
BstBAI YACGTR 1 cut(s) 189
BstDEI CTNAG 7 cut(s) 330, 698, 711, 752, 852, 1238, 1277
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 4 cut(s) 307, 574, 838, 933
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 5 cut(s) 157, 362, 529, 824, 1195
BstMAI GTCTC 3 cut(s) 40, 858, 1057
BstMBI GATC 5 cut(s) 154, 359, 526, 821, 1192
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 4 cut(s) 28, 323, 797, 1055
BstNI CCWGG 2 cut(s) 726, 783
BstNSI RCATGY 1 cut(s) 593
BstPAI GACNNNNGTC 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 724, 781
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 3 cut(s) 892, 1028, 1063
BstV2I GAAGAC 1 cut(s) 1024
BstX2I RGATCY 1 cut(s) 821
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 821
BstZ17I GTATAC 1 cut(s) 603
BsuRI GGCC 5 cut(s) 22, 474, 718, 910, 990
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 4 cut(s) 307, 574, 838, 933
BtsIMutI CAGTG 4 cut(s) 327, 328, 544, 918
BveI ACCTGC 2 cut(s) 538, 791
CciI TCATGA 1 cut(s) 1159
Cfr13I GGNCC 4 cut(s) 54, 218, 716, 785
CpoI CGGWCCG 1 cut(s) 218
Csp6I GTAC 2 cut(s) 190, 874
CspI CGGWCCG 1 cut(s) 218
CviAII CATG 6 cut(s) 277, 399, 590, 662, 1085, 1160
CviQI GTAC 2 cut(s) 190, 874
DdeI CTNAG 7 cut(s) 330, 698, 711, 752, 852, 1238, 1277
DpnI GATC 5 cut(s) 156, 361, 528, 823, 1194
DpnII GATC 5 cut(s) 154, 359, 526, 821, 1192
EaeI YGGCCR 2 cut(s) 472, 908
Eam1104I CTCTTC 1 cut(s) 1094
EarI CTCTTC 1 cut(s) 1094
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 276, 469
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 1 cut(s) 858
Eco47I GGWCC 3 cut(s) 54, 218, 785
EcoRII CCWGG 2 cut(s) 724, 781
EcoT14I CCWWGG 2 cut(s) 276, 469
EcoT22I ATGCAT 1 cut(s) 1308
EcoT38I GRGCYC 1 cut(s) 930
ErhI CCWWGG 2 cut(s) 276, 469
FaeI CATG 6 cut(s) 280, 402, 593, 665, 1088, 1163
FaqI GGGAC 1 cut(s) 174
FatI CATG 6 cut(s) 276, 398, 589, 661, 1084, 1159
FbaI TGATCA 1 cut(s) 1192
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 8 cut(s) 5, 61, 881, 908, 1017, 1077, 1092, 1151
FokI GGATG 4 cut(s) 314, 561, 845, 920
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 8 cut(s) 5, 61, 881, 908, 1017, 1077, 1092, 1151
FspBI CTAG 3 cut(s) 231, 1028, 1044
GluI GCNGC 8 cut(s) 5, 61, 881, 908, 1017, 1077, 1092, 1151
HaeIII GGCC 5 cut(s) 22, 474, 718, 910, 990
HapII CCGG 4 cut(s) 57, 108, 221, 475
Hin1II CATG 6 cut(s) 280, 402, 593, 665, 1088, 1163
HincII GTYRAC 3 cut(s) 241, 522, 1264
HindII GTYRAC 3 cut(s) 241, 522, 1264
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 234, 445, 455, 584
HpaII CCGG 4 cut(s) 57, 108, 221, 475
HphI GGTGA 5 cut(s) 77, 122, 364, 928, 1240
Hpy166II GTNNAC 7 cut(s) 171, 241, 484, 522, 603, 704, 1264
Hpy188I TCNGA 2 cut(s) 753, 985
Hpy188III TCNNGA 8 cut(s) 231, 459, 553, 578, 819, 1036, 1160, 1190
Hpy8I GTNNAC 7 cut(s) 171, 241, 484, 522, 603, 704, 1264
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 4 cut(s) 502, 516, 1002, 1174
HpyCH4IV ACGT 2 cut(s) 188, 192
HpyCH4V TGCA 7 cut(s) 673, 731, 760, 800, 980, 1283, 1306
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 323, 797, 1055
HpyF3I CTNAG 7 cut(s) 330, 698, 711, 752, 852, 1238, 1277
HpySE526I ACGT 2 cut(s) 188, 192
Hsp92II CATG 6 cut(s) 280, 402, 593, 665, 1088, 1163
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 5 cut(s) 154, 359, 526, 821, 1192
LmnI GCTCC 1 cut(s) 1211
Lsp1109I GCAGC 3 cut(s) 892, 1028, 1063
LweI GCATC 3 cut(s) 800, 989, 1315
MaeI CTAG 3 cut(s) 231, 1028, 1044
MaeII ACGT 2 cut(s) 188, 192
MaeIII GTNAC 3 cut(s) 110, 209, 916
MalI GATC 5 cut(s) 156, 361, 528, 823, 1194
MbiI CCGCTC 1 cut(s) 31
MboI GATC 5 cut(s) 154, 359, 526, 821, 1192
MboII GAAGA 2 cut(s) 1024, 1111
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 821
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 9 cut(s) 139, 269, 381, 633, 667, 693, 740, 939, 1131
Mph1103I ATGCAT 1 cut(s) 1308
MroXI GAANNNNTTC 2 cut(s) 317, 570
MseI TTAA 3 cut(s) 858, 951, 1317
MslI CAYNNNNRTG 1 cut(s) 1158
MspI CCGG 4 cut(s) 57, 108, 221, 475
MspR9I CCNGG 2 cut(s) 726, 783
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 2 cut(s) 726, 783
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 4 cut(s) 28, 323, 797, 1055
NcoI CCATGG 1 cut(s) 276
NdeII GATC 5 cut(s) 154, 359, 526, 821, 1192
NlaIII CATG 6 cut(s) 280, 402, 593, 665, 1088, 1163
NlaIV GGNNCC 2 cut(s) 55, 717
NmuCI GTSAC 3 cut(s) 110, 209, 916
NsiI ATGCAT 1 cut(s) 1308
NspI RCATGY 1 cut(s) 593
PagI TCATGA 1 cut(s) 1159
PciI ACATGT 1 cut(s) 589
PdmI GAANNNNTTC 2 cut(s) 317, 570
PfeI GAWTC 4 cut(s) 234, 445, 455, 584
Pfl23II CGTACG 1 cut(s) 189
PflMI CCANNNNNTGG 1 cut(s) 725
PkrI GCNGC 8 cut(s) 6, 62, 882, 909, 1018, 1078, 1093, 1152
Ple19I CGATCG 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 189
PscI ACATGT 1 cut(s) 589
PshAI GACNNNNGTC 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 724, 781
PspGI CCWGG 2 cut(s) 724, 781
PspLI CGTACG 1 cut(s) 189
PspN4I GGNNCC 2 cut(s) 55, 717
PspPI GGNCC 4 cut(s) 54, 218, 716, 785
PsuI RGATCY 1 cut(s) 821
PvuI CGATCG 1 cut(s) 362
RsaI GTAC 2 cut(s) 191, 875
RsaNI GTAC 2 cut(s) 190, 874
RseI CAYNNNNRTG 1 cut(s) 1158
Rsr2I CGGWCCG 1 cut(s) 218
RsrII CGGWCCG 1 cut(s) 218
SaqAI TTAA 3 cut(s) 858, 951, 1317
SatI GCNGC 8 cut(s) 5, 61, 881, 908, 1017, 1077, 1092, 1151
Sau3AI GATC 5 cut(s) 154, 359, 526, 821, 1192
Sau96I GGNCC 4 cut(s) 54, 218, 716, 785
ScrFI CCNGG 2 cut(s) 726, 783
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 3 cut(s) 800, 989, 1315
SinI GGWCC 3 cut(s) 54, 218, 785
SmiMI CAYNNNNRTG 1 cut(s) 1158
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 9 cut(s) 139, 269, 381, 633, 667, 693, 740, 939, 1131
SspI AATATT 2 cut(s) 688, 767
SspMI CTAG 3 cut(s) 231, 1028, 1044
StyD4I CCNGG 2 cut(s) 724, 781
StyI CCWWGG 2 cut(s) 276, 469
TaaI ACNGT 4 cut(s) 502, 516, 1002, 1174
TaiI ACGT 2 cut(s) 191, 195
TaqI TCGA 4 cut(s) 17, 119, 143, 458
TasI AATT 9 cut(s) 139, 269, 381, 633, 667, 693, 740, 939, 1131
TauI GCSGC 4 cut(s) 63, 910, 1094, 1153
TfiI GAWTC 4 cut(s) 234, 445, 455, 584
Tru1I TTAA 3 cut(s) 858, 951, 1317
Tru9I TTAA 3 cut(s) 858, 951, 1317
TscAI CASTG 4 cut(s) 328, 334, 544, 918
TseFI GTSAC 3 cut(s) 110, 209, 916
TseI GCWGC 4 cut(s) 4, 880, 1016, 1076
Tsp45I GTSAC 3 cut(s) 110, 209, 916
TspDTI ATGAA 5 cut(s) 386, 563, 917, 1101, 1148
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 4 cut(s) 328, 334, 544, 918
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 3 cut(s) 54, 218, 785
XapI RAATTY 4 cut(s) 139, 269, 693, 740
XbaI TCTAGA 1 cut(s) 230
XceI RCATGY 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 2 cut(s) 317, 570
XspI CTAG 3 cut(s) 231, 1028, 1044
Zsp2I ATGCAT 1 cut(s) 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.