pycom15g25550

protein phosphatase 2C 78

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001678
Physical Location & Seq
Reverse (-)
20067788 .. 20070669
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g25550.1

Sequence Viewer

Length: 1269 bp
ATGAACCCCACGTCCCTCTTTTATTCCAACACAACACCCCAACTACCAAACCAAAACCCGCGAATTCCTCTTCCTCTTTTTCCCCAAAACCATAGGGAAAATCCAAGTACAACCAGAGAACCCAAGCTGACAATGATGTTGGACATGTGTAGGAGGCCGTTGGAGAAGTGTTTTGGTGGCGGAGACGGCGGCGATGGCCTGCTTTGGCACATGGACCTCAAGCCACACACTTCTGGGGACTATTCGATTGCAGTGGTTCAAGCAAATTCGGCGCTGGAAGATCAGGGACAGGTGTTCTCATCTCCTTTCGCAACGTACGTTGGAGTCTACGACGGCCATGGCGGTCCTGAAGCTTCCCGCTTCATTACCAACCGACTCTTCCCCTTTCTTCACAAGTTTGCAACCGAACAAGGTGGACTGTCGGAGGATGTGATAAGGCAGGCATTTGATGCAACAGAGGAGGAGTTCTTGGAGTTGGTTAAGCGATCATGGATGGTGCGGCCGCAGATTGCTTCAGTAGGATCGTGTTGCCTGGTTGGTGCAATTGCGGATGGTCTTTTACATGTGGCTAATCTCGGAGATTCGAGGGCAGTCCTTGGCAGGAGATCATCGGATGGGCAGGCGGTGGTGGCAGAGCGGTTGTCTACGGATCACAATGTTGCAGTGGAGGAAGTGAGGAAGGAGGTTAAGGATCTTCACCCTGATGATGCGCACATTGTGGTGTTTACGCGTGGAGTTTGGCGAATTAAGGGCATTATTCAGGTCTCAAGGTCAATTGGTGATGTCTACTTGAAGAAACCTGAGTTTAACAGAGATCCTCTCTTTCACCACTTTGGCATGCCCGTTCCTTTAAAGAAGCCTGTAATGACAGCAGAACCCTCTATAGTAGTTCGAAAGTTACAGCCACAGGACATGTTTTTGATATTTGCAACAGACGGTCTCTGGGAGCATTTGAATGATGAAACAGCCGTGAAAATCGTCTCCAAAAATCCAAGAGCTGGAATAGCAAAGCGATTGGTAAGAACTGCCATCGGTGAGGCTGCAAGGAAAAGACAATTGAGATATGACGATATAAGAAGAATTGAAAGGGGGGTAAGGCGCCATTTCCACGACGATATCACTGTAATAGTGATATTCCTAGATCATTCACAAGTTTCCTCAAATGCTAGTCTCAAGGATGAGAGCCTCTTTAACTACACTAATGTCCCTGTTGATATATTTTCAATGAACGAAGAAGCAGCAGACGGATCACTCCACACCTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

423

Amino Acids

47.27

Weight (kDa)

6.47

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 105 - 349 8.2e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 711
AccB1I GGYRCC 1 cut(s) 1098
AccB7I CCANNNNNTGG 1 cut(s) 998
AccBSI CCGCTC 1 cut(s) 637
AccI GTMKAC 3 cut(s) 327, 644, 786
AccII CGCG 2 cut(s) 61, 730
AclWI GGATC 5 cut(s) 529, 657, 699, 809, 1255
AcoI YGGCCR 2 cut(s) 334, 500
AcsI RAATTY 2 cut(s) 63, 265
AcuI CTGAAG 2 cut(s) 369, 498
AcyI GRCGYC 1 cut(s) 1099
AdeI CACNNNGTG 1 cut(s) 718
AfaI GTAC 2 cut(s) 109, 317
AfiI CCNNNNNNNGG 1 cut(s) 998
AflIII ACRYGT 4 cut(s) 144, 562, 728, 912
AgsI TTSAA 5 cut(s) 260, 793, 955, 1085, 1224
AjiI CACGTC 1 cut(s) 12
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 3 cut(s) 127, 353, 998
AluI AGCT 3 cut(s) 127, 353, 998
Alw26I GTCTC 5 cut(s) 177, 769, 944, 985, 1175
AlwI GGATC 5 cut(s) 529, 657, 699, 809, 1255
AoxI GGCC 4 cut(s) 155, 196, 334, 500
ApeKI GCWGC 2 cut(s) 1040, 1238
ApoI RAATTY 2 cut(s) 63, 265
AspLEI GCGC 3 cut(s) 274, 712, 1101
AspS9I GGNCC 2 cut(s) 214, 344
AsuHPI GGTGA 4 cut(s) 689, 791, 818, 1046
AsuII TTCGAA 1 cut(s) 892
AvaII GGWCC 2 cut(s) 214, 344
BanI GGYRCC 1 cut(s) 1098
BbvI GCAGC 2 cut(s) 1027, 1250
BccI CCATC 5 cut(s) 188, 487, 545, 608, 1037
BceAI ACGGC 4 cut(s) 142, 202, 349, 953
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 5 cut(s) 177, 769, 944, 985, 1175
BfaI CTAG 2 cut(s) 1139, 1167
BfmI CTRYAG 1 cut(s) 882
BfoI RGCGCY 2 cut(s) 275, 1102
BisI GCNGC 5 cut(s) 190, 500, 503, 1041, 1239
BlsI GCNGC 5 cut(s) 191, 501, 504, 1042, 1240
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 2 cut(s) 214, 344
BmgBI CACGTC 1 cut(s) 12
BmgT120I GGNCC 2 cut(s) 214, 344
BmiI GGNNCC 1 cut(s) 1100
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 2 cut(s) 439, 697
BplI GAGNNNNNCTC 2 cut(s) 804, 836
Bpu14I TTCGAA 1 cut(s) 892
BpuEI CTTGAG 3 cut(s) 203, 751, 1157
BsaHI GRCGYC 1 cut(s) 1099
BsaI GGTCTC 2 cut(s) 769, 944
BsaJI CCNNGG 2 cut(s) 337, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 998
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 337, 595
BseGI GGATG 5 cut(s) 433, 498, 556, 619, 1183
BseLI CCNNNNNNNGG 1 cut(s) 998
BseMII CTCAG 1 cut(s) 792
BseRI GAGGAG 2 cut(s) 473, 476
BseX3I CGGCCG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 1027, 1250
Bsh1236I CGCG 2 cut(s) 61, 730
Bsh1285I CGRYCG 1 cut(s) 503
BshFI GGCC 4 cut(s) 157, 198, 336, 502
BshNI GGYRCC 1 cut(s) 1098
BsiEI CGRYCG 1 cut(s) 503
BsiWI CGTACG 1 cut(s) 315
BslFI GGGAC 3 cut(s) 251, 300, 1190
BslI CCNNNNNNNGG 1 cut(s) 998
BsmAI GTCTC 5 cut(s) 177, 769, 944, 985, 1175
BsmBI CGTCTC 2 cut(s) 177, 985
BsmFI GGGAC 3 cut(s) 251, 300, 1190
BsnI GGCC 4 cut(s) 157, 198, 336, 502
Bso31I GGTCTC 2 cut(s) 769, 944
Bsp119I TTCGAA 1 cut(s) 892
Bsp143I GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
Bsp19I CCATGG 1 cut(s) 337
BspANI GGCC 4 cut(s) 157, 198, 336, 502
BspCNI CTCAG 1 cut(s) 793
BspFNI CGCG 2 cut(s) 61, 730
BspLI GGNNCC 1 cut(s) 1100
BspPI GGATC 5 cut(s) 529, 657, 699, 809, 1255
BspT104I TTCGAA 1 cut(s) 892
BspT107I GGYRCC 1 cut(s) 1098
BspTNI GGTCTC 2 cut(s) 769, 944
BsrBI CCGCTC 1 cut(s) 637
BssECI CCNNGG 2 cut(s) 337, 595
BssMI GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
BssNI GRCGYC 1 cut(s) 1099
BssT1I CCWWGG 2 cut(s) 337, 595
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 420, 938, 1123
Bst6I CTCTTC 2 cut(s) 75, 383
BstACI GRCGYC 1 cut(s) 1099
BstAPI GCANNNNNTGC 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 892
BstC8I GCNNGC 4 cut(s) 200, 441, 621, 839
BstDEI CTNAG 1 cut(s) 801
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 5 cut(s) 433, 498, 556, 619, 1183
BstFNI CGCG 2 cut(s) 61, 730
BstH2I RGCGCY 2 cut(s) 275, 1102
BstHHI GCGC 3 cut(s) 274, 712, 1101
BstKTI GATC 9 cut(s) 283, 488, 524, 608, 652, 694, 817, 1144, 1250
BstMAI GTCTC 5 cut(s) 177, 769, 944, 985, 1175
BstMBI GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
BstMCI CGRYCG 1 cut(s) 503
BstMWI GCNNNNNNNGC 6 cut(s) 186, 195, 269, 449, 629, 1004
BstNI CCWGG 1 cut(s) 533
BstNSI RCATGY 4 cut(s) 148, 566, 841, 916
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 882
BstUI CGCG 2 cut(s) 61, 730
BstV1I GCAGC 2 cut(s) 1027, 1250
BstX2I RGATCY 2 cut(s) 691, 814
BstYI RGATCY 2 cut(s) 691, 814
BstZI CGGCCG 1 cut(s) 500
BsuRI GGCC 4 cut(s) 157, 198, 336, 502
BtgI CCRYGG 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 12
BtsCI GGATG 5 cut(s) 433, 498, 556, 619, 1183
BtsI GCAGTG 2 cut(s) 258, 669
BtsIMutI CAGTG 3 cut(s) 258, 669, 1119
Cac8I GCNNGC 4 cut(s) 200, 441, 621, 839
CciNI GCGGCCGC 1 cut(s) 500
CfoI GCGC 3 cut(s) 274, 712, 1101
Cfr13I GGNCC 2 cut(s) 214, 344
Csp6I GTAC 2 cut(s) 108, 316
CviAII CATG 8 cut(s) 145, 211, 338, 489, 563, 838, 913, 1266
CviQI GTAC 2 cut(s) 108, 316
DdeI CTNAG 1 cut(s) 801
DinI GGCGCC 1 cut(s) 1100
DpnI GATC 9 cut(s) 282, 487, 523, 607, 651, 693, 816, 1143, 1249
DpnII GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
DraI TTTAAA 1 cut(s) 852
DraIII CACNNNGTG 1 cut(s) 718
EaeI YGGCCR 2 cut(s) 334, 500
EagI CGGCCG 1 cut(s) 500
Eam1104I CTCTTC 2 cut(s) 75, 383
EarI CTCTTC 2 cut(s) 75, 383
EciI GGCGGA 1 cut(s) 195
EclXI CGGCCG 1 cut(s) 500
Eco130I CCWWGG 2 cut(s) 337, 595
Eco31I GGTCTC 2 cut(s) 769, 944
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 2 cut(s) 214, 344
Eco52I CGGCCG 1 cut(s) 500
Eco57I CTGAAG 2 cut(s) 369, 498
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 1117
EcoT14I CCWWGG 2 cut(s) 337, 595
EgeI GGCGCC 1 cut(s) 1100
EheI GGCGCC 1 cut(s) 1100
ErhI CCWWGG 2 cut(s) 337, 595
Esp3I CGTCTC 2 cut(s) 177, 985
FaeI CATG 8 cut(s) 148, 214, 341, 492, 566, 841, 916, 1269
FaqI GGGAC 3 cut(s) 251, 300, 1190
FatI CATG 8 cut(s) 144, 210, 337, 488, 562, 837, 912, 1265
FauI CCCGC 2 cut(s) 66, 365
FblI GTMKAC 3 cut(s) 327, 644, 786
Fnu4HI GCNGC 5 cut(s) 190, 500, 503, 1041, 1239
FokI GGATG 5 cut(s) 440, 505, 563, 626, 1190
Fsp4HI GCNGC 5 cut(s) 190, 500, 503, 1041, 1239
FspAI RTGCGCAY 1 cut(s) 711
FspBI CTAG 2 cut(s) 1139, 1167
FspI TGCGCA 1 cut(s) 711
GlaI GCGC 3 cut(s) 273, 711, 1100
GluI GCNGC 5 cut(s) 190, 500, 503, 1041, 1239
HaeII RGCGCY 2 cut(s) 275, 1102
HaeIII GGCC 4 cut(s) 157, 198, 336, 502
HhaI GCGC 3 cut(s) 274, 712, 1101
Hin1I GRCGYC 1 cut(s) 1099
Hin1II CATG 8 cut(s) 148, 214, 341, 492, 566, 841, 916, 1269
Hin6I GCGC 3 cut(s) 272, 710, 1099
HinP1I GCGC 3 cut(s) 272, 710, 1099
HindIII AAGCTT 1 cut(s) 351
HinfI GANTC 3 cut(s) 324, 375, 581
HphI GGTGA 4 cut(s) 689, 791, 818, 1046
Hpy166II GTNNAC 5 cut(s) 328, 416, 645, 726, 787
Hpy188I TCNGA 3 cut(s) 424, 578, 613
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 5 cut(s) 328, 416, 645, 726, 787
Hpy99I CGWCG 2 cut(s) 335, 1115
HpyAV CCTTC 1 cut(s) 673
HpyCH4III ACNGT 3 cut(s) 420, 938, 1123
HpyCH4IV ACGT 3 cut(s) 11, 314, 318
HpyCH4V TGCA 7 cut(s) 251, 401, 452, 542, 662, 929, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 186, 195, 269, 449, 629, 1004
HpyF3I CTNAG 1 cut(s) 801
HpySE526I ACGT 3 cut(s) 11, 314, 318
Hsp92I GRCGYC 1 cut(s) 1099
Hsp92II CATG 8 cut(s) 148, 214, 341, 492, 566, 841, 916, 1269
HspAI GCGC 3 cut(s) 272, 710, 1099
KasI GGCGCC 1 cut(s) 1098
Kzo9I GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
LmnI GCTCC 1 cut(s) 946
Lsp1109I GCAGC 2 cut(s) 1027, 1250
LweI GCATC 2 cut(s) 439, 697
MaeI CTAG 2 cut(s) 1139, 1167
MaeII ACGT 3 cut(s) 11, 314, 318
MaeIII GTNAC 1 cut(s) 897
MalI GATC 9 cut(s) 282, 487, 523, 607, 651, 693, 816, 1143, 1249
MbiI CCGCTC 1 cut(s) 637
MboI GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
MboII GAAGA 8 cut(s) 62, 290, 370, 380, 686, 805, 1089, 1244
MfeI CAATTG 3 cut(s) 543, 774, 1055
MflI RGATCY 2 cut(s) 691, 814
MluCI AATT 7 cut(s) 63, 265, 543, 744, 774, 1055, 1080
MluI ACGCGT 1 cut(s) 728
Mly113I GGCGCC 1 cut(s) 1099
MlyI GAGTC 2 cut(s) 333, 369
MmeI TCCRAC 5 cut(s) 51, 120, 141, 301, 402
MseI TTAA 6 cut(s) 480, 687, 747, 807, 851, 1191
MslI CAYNNNNRTG 3 cut(s) 702, 719, 954
MspR9I CCNGG 1 cut(s) 533
MunI CAATTG 3 cut(s) 543, 774, 1055
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 2 cut(s) 61, 730
MwoI GCNNNNNNNGC 6 cut(s) 186, 195, 269, 449, 629, 1004
NarI GGCGCC 1 cut(s) 1099
NcoI CCATGG 1 cut(s) 337
NdeII GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
NlaIII CATG 8 cut(s) 148, 214, 341, 492, 566, 841, 916, 1269
NlaIV GGNNCC 1 cut(s) 1100
NotI GCGGCCGC 1 cut(s) 500
NsbI TGCGCA 1 cut(s) 711
NspI RCATGY 4 cut(s) 148, 566, 841, 916
NspV TTCGAA 1 cut(s) 892
PaeI GCATGC 1 cut(s) 841
PciI ACATGT 3 cut(s) 144, 562, 912
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 1 cut(s) 581
Pfl23II CGTACG 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 998
PkrI GCNGC 5 cut(s) 191, 501, 504, 1042, 1240
PleI GAGTC 2 cut(s) 332, 369
PluTI GGCGCC 1 cut(s) 1102
PpsI GAGTC 2 cut(s) 332, 369
PscI ACATGT 3 cut(s) 144, 562, 912
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspLI CGTACG 1 cut(s) 315
PspN4I GGNNCC 1 cut(s) 1100
PspPI GGNCC 2 cut(s) 214, 344
PsuI RGATCY 2 cut(s) 691, 814
RsaI GTAC 2 cut(s) 109, 317
RsaNI GTAC 2 cut(s) 108, 316
RseI CAYNNNNRTG 3 cut(s) 702, 719, 954
SaqAI TTAA 6 cut(s) 480, 687, 747, 807, 851, 1191
SatI GCNGC 5 cut(s) 190, 500, 503, 1041, 1239
Sau3AI GATC 9 cut(s) 280, 485, 521, 605, 649, 691, 814, 1141, 1247
Sau96I GGNCC 2 cut(s) 214, 344
SchI GAGTC 2 cut(s) 333, 369
ScrFI CCNGG 1 cut(s) 533
SfaNI GCATC 2 cut(s) 439, 697
SfcI CTRYAG 1 cut(s) 882
SfoI GGCGCC 1 cut(s) 1100
SfuI TTCGAA 1 cut(s) 892
SinI GGWCC 2 cut(s) 214, 344
SmiMI CAYNNNNRTG 3 cut(s) 702, 719, 954
SmlI CTYRAG 3 cut(s) 218, 766, 1172
SmoI CTYRAG 3 cut(s) 218, 766, 1172
SphI GCATGC 1 cut(s) 841
Sse9I AATT 7 cut(s) 63, 265, 543, 744, 774, 1055, 1080
SspDI GGCGCC 1 cut(s) 1098
SspMI CTAG 2 cut(s) 1139, 1167
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 2 cut(s) 337, 595
TaaI ACNGT 3 cut(s) 420, 938, 1123
TaiI ACGT 3 cut(s) 14, 317, 321
TaqI TCGA 3 cut(s) 245, 584, 892
TasI AATT 7 cut(s) 63, 265, 543, 744, 774, 1055, 1080
TatI WGTACW 1 cut(s) 107
TauI GCSGC 3 cut(s) 192, 502, 505
TfiI GAWTC 1 cut(s) 581
Tru1I TTAA 6 cut(s) 480, 687, 747, 807, 851, 1191
Tru9I TTAA 6 cut(s) 480, 687, 747, 807, 851, 1191
TscAI CASTG 3 cut(s) 258, 669, 1126
TseI GCWGC 2 cut(s) 1040, 1238
TspDTI ATGAA 4 cut(s) 17, 352, 975, 1241
TspGWI ACGGA 2 cut(s) 662, 1260
TspRI CASTG 3 cut(s) 258, 669, 1126
Van91I CCANNNNNTGG 1 cut(s) 998
VpaK11BI GGWCC 2 cut(s) 214, 344
XapI RAATTY 2 cut(s) 63, 265
XceI RCATGY 4 cut(s) 148, 566, 841, 916
XmiI GTMKAC 3 cut(s) 327, 644, 786
XspI CTAG 2 cut(s) 1139, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.