RchiOBHm_Chr6g0254141

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001678
Physical Location & Seq
Forward (+)
9197636 .. 9200318
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22794

Sequence Viewer

Length: 1149 bp
ATGCTGCGGACGATTTCGAGGCCGTTAGAGCGGTGTTTTGGGTTGAGACTTGGGTCCGGCGGCGGTGATGGGTTGCTTTGGCATACCGACTTGAAGCCTCACGCCTCCGGTGACTACTCGATCGCTGTTGTTCAGGCAAATTCGAGCCTTGAGGATCAGGGACAGGTGTTCACCTCTCCCTCTGCCACGTACGTCGGCGTTTATGACGGTCACGGCGGACCGGAAGCTTCTAGATTCGTCAACAATAATCTGTTCCCGTTTTTACACAAATTTGCCATGGAACAAGGTGGGTTATCTGCGGATGTGATAAGGAAGGCGTTCAGTGCCACTGAGGAGGAGTTTCTGCGTCTGGTGAAGCGATCGTGGCCGATGAAGCCGTCAATTGCTTCTGTCGGGTCATGTTGTTTGGTCGGGGCTATAACAAATGATGTTCTGTATGTTGCGAATCTGGGGGATTCGAGAGCAGTCCTTGGCCGGAAAGTGAACGGGAGTAAGAGGACGGTGGTGGCAGAACGGTTGTCAACGGATCACAATGTCAGTGATGAGCAGGTCAGGAGGGAAGTTGAAGCACTTCATCCTGACGATTCACATGTTGTGGTGTATACTCGCGGAGTTTGGAGGATAAAGGGGATAATTCAGGTGTCAAGATCTATTGGGGATGTTTATCTAAAGAAGCCTGAGTTTAACAGAGACCCAATGTACCAGCAGTTTATTACCCCTGTTCCTATGAAGCGGCCAGTGGTGACAGCAGAGCCCTCCATCCTTATTAGAAGCATTAAGCCACAGGATATGTTTTTGATATTCGCATCAGATGGCCTATGGGAACAGTTGAGTGATGAGGCAGCAGTAGAGCTAGTCTTCAAGAACCCTAGAGCTGGTATTGCCAAGAGACTGGTAAGAGCTGCTCTCCATGAAGCCGCCAAGAAGAGGGAAATGAGATATGATGACATAAAGAAAATTGAAAAGGGAATAAGGCGGCATTTTCATGATGATATTACTGTCATTGTAGTCTATCTTGATCAGCACAAAAGCTCCTCAAAACATAAACTCGGCAACAATGCTCTAAGTTCCATCACCCAAGCACCTGTTGACATTTACTCGCTTAGTGCAGAGGAAGTAGAACAGGATATGCATCATACCATTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

382

Amino Acids

42.63

Weight (kDa)

8.78

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 60 - 308 1.5e-40 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 538
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 602
AccII CGCG 1 cut(s) 609
AclWI GGATC 2 cut(s) 162, 534
AcoI YGGCCR 3 cut(s) 365, 472, 734
AcsI RAATTY 2 cut(s) 139, 269
AfaI GTAC 2 cut(s) 191, 701
AfiI CCNNNNNNNGG 2 cut(s) 875, 927
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 4 cut(s) 94, 566, 862, 962
AluBI AGCT 5 cut(s) 227, 853, 875, 902, 1032
AluI AGCT 5 cut(s) 227, 853, 875, 902, 1032
Alw26I GTCTC 3 cut(s) 40, 684, 883
AlwI GGATC 2 cut(s) 162, 534
AoxI GGCC 5 cut(s) 20, 365, 472, 734, 814
ApeKI GCWGC 3 cut(s) 4, 842, 902
ApoI RAATTY 2 cut(s) 139, 269
Asp700I GAANNNNTTC 2 cut(s) 317, 570
AspS9I GGNCC 2 cut(s) 54, 218
AsuHPI GGTGA 6 cut(s) 77, 122, 163, 364, 754, 1066
AvaII GGWCC 2 cut(s) 54, 218
BanII GRGCYC 1 cut(s) 756
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 2 cut(s) 854, 889
BccI CCATC 4 cut(s) 62, 767, 806, 1079
BceAI ACGGC 3 cut(s) 7, 229, 361
BclI TGATCA 1 cut(s) 1018
BcoDI GTCTC 3 cut(s) 40, 684, 883
BfaI CTAG 3 cut(s) 231, 854, 870
BfuAI ACCTGC 1 cut(s) 538
BglII AGATCT 1 cut(s) 647
BisI GCNGC 7 cut(s) 5, 61, 734, 843, 903, 918, 977
BlsI GCNGC 7 cut(s) 6, 62, 735, 844, 904, 919, 978
Bme18I GGWCC 2 cut(s) 54, 218
BmgT120I GGNCC 2 cut(s) 54, 218
BmiI GGNNCC 1 cut(s) 55
BmsI GCATC 2 cut(s) 815, 1141
BoxI GACNNNNGTC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 850
BplI GAGNNNNNCTC 2 cut(s) 891, 923
BpuEI CTTGAG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 189
BsaBI GATNNNNATC 2 cut(s) 663, 1131
BsaI GGTCTC 1 cut(s) 684
BsaJI CCNNGG 2 cut(s) 276, 469
BsaWI WCCGGW 2 cut(s) 107, 220
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bsc4I CCNNNNNNNGG 2 cut(s) 875, 927
Bse1I ACTGG 2 cut(s) 737, 897
Bse8I GATNNNNATC 2 cut(s) 663, 1131
BseDI CCNNGG 2 cut(s) 276, 469
BseGI GGATG 4 cut(s) 307, 574, 664, 759
BseJI GATNNNNATC 2 cut(s) 663, 1131
BseLI CCNNNNNNNGG 2 cut(s) 875, 927
BseMII CTCAG 2 cut(s) 321, 669
BseNI ACTGG 2 cut(s) 737, 897
BseRI GAGGAG 3 cut(s) 347, 350, 1024
BseXI GCAGC 2 cut(s) 854, 889
BsgI GTGCAG 1 cut(s) 1128
Bsh1236I CGCG 1 cut(s) 609
Bsh1285I CGRYCG 2 cut(s) 123, 362
BshFI GGCC 5 cut(s) 22, 367, 474, 736, 816
BsiEI CGRYCG 2 cut(s) 123, 362
BsiSI CCGG 4 cut(s) 57, 108, 221, 475
BsiWI CGTACG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 875, 927
BsmAI GTCTC 3 cut(s) 40, 684, 883
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 5 cut(s) 22, 367, 474, 736, 816
Bso31I GGTCTC 1 cut(s) 684
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
Bsp19I CCATGG 1 cut(s) 276
BspANI GGCC 5 cut(s) 22, 367, 474, 736, 816
BspCNI CTCAG 2 cut(s) 322, 670
BspFNI CGCG 1 cut(s) 609
BspHI TCATGA 1 cut(s) 985
BspLI GGNNCC 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 2 cut(s) 162, 534
BspTNI GGTCTC 1 cut(s) 684
BsrBI CCGCTC 1 cut(s) 31
BsrI ACTGG 2 cut(s) 737, 897
BssECI CCNNGG 2 cut(s) 276, 469
BssMI GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
BssNAI GTATAC 1 cut(s) 603
BssT1I CCWWGG 2 cut(s) 276, 469
Bst1107I GTATAC 1 cut(s) 603
Bst4CI ACNGT 5 cut(s) 209, 502, 516, 828, 1000
Bst6I CTCTTC 1 cut(s) 920
BstBAI YACGTR 1 cut(s) 189
BstDEI CTNAG 4 cut(s) 330, 678, 1064, 1103
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 4 cut(s) 307, 574, 664, 759
BstFNI CGCG 1 cut(s) 609
BstKTI GATC 6 cut(s) 123, 157, 362, 529, 650, 1021
BstMAI GTCTC 3 cut(s) 40, 684, 883
BstMBI GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
BstMCI CGRYCG 2 cut(s) 123, 362
BstMWI GCNNNNNNNGC 5 cut(s) 28, 323, 364, 373, 881
BstNSI RCATGY 1 cut(s) 593
BstPAI GACNNNNGTC 1 cut(s) 52
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 2 cut(s) 854, 889
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 1 cut(s) 647
BstZ17I GTATAC 1 cut(s) 603
BsuRI GGCC 5 cut(s) 22, 367, 474, 736, 816
BtgI CCRYGG 1 cut(s) 276
BtsCI GGATG 4 cut(s) 307, 574, 664, 759
BtsIMutI CAGTG 4 cut(s) 327, 328, 544, 744
BveI ACCTGC 1 cut(s) 538
CciI TCATGA 1 cut(s) 985
Cfr13I GGNCC 2 cut(s) 54, 218
CpoI CGGWCCG 1 cut(s) 218
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 190, 700
CspI CGGWCCG 1 cut(s) 218
CviAII CATG 5 cut(s) 277, 399, 590, 911, 986
CviQI GTAC 2 cut(s) 190, 700
DdeI CTNAG 4 cut(s) 330, 678, 1064, 1103
DpnI GATC 6 cut(s) 122, 156, 361, 528, 649, 1020
DpnII GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
EaeI YGGCCR 3 cut(s) 365, 472, 734
Eam1104I CTCTTC 1 cut(s) 920
EarI CTCTTC 1 cut(s) 920
EciI GGCGGA 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 276, 469
Eco24I GRGCYC 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 684
Eco47I GGWCC 2 cut(s) 54, 218
EcoT14I CCWWGG 2 cut(s) 276, 469
EcoT22I ATGCAT 1 cut(s) 1134
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 2 cut(s) 276, 469
FaeI CATG 5 cut(s) 280, 402, 593, 914, 989
FaqI GGGAC 1 cut(s) 174
FatI CATG 5 cut(s) 276, 398, 589, 910, 985
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 7 cut(s) 5, 61, 734, 843, 903, 918, 977
FokI GGATG 4 cut(s) 314, 561, 671, 746
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 7 cut(s) 5, 61, 734, 843, 903, 918, 977
FspBI CTAG 3 cut(s) 231, 854, 870
GluI GCNGC 7 cut(s) 5, 61, 734, 843, 903, 918, 977
HaeIII GGCC 5 cut(s) 22, 367, 474, 736, 816
HapII CCGG 4 cut(s) 57, 108, 221, 475
HgaI GACGC 1 cut(s) 335
Hin1II CATG 5 cut(s) 280, 402, 593, 914, 989
HincII GTYRAC 3 cut(s) 241, 522, 1090
HindII GTYRAC 3 cut(s) 241, 522, 1090
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 4 cut(s) 234, 445, 455, 584
HpaII CCGG 4 cut(s) 57, 108, 221, 475
HphI GGTGA 6 cut(s) 77, 122, 163, 364, 754, 1066
Hpy166II GTNNAC 6 cut(s) 171, 241, 484, 522, 603, 1090
Hpy188I TCNGA 1 cut(s) 811
Hpy188III TCNNGA 8 cut(s) 231, 459, 553, 578, 645, 862, 986, 1016
Hpy8I GTNNAC 6 cut(s) 171, 241, 484, 522, 603, 1090
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 5 cut(s) 209, 502, 516, 828, 1000
HpyCH4IV ACGT 2 cut(s) 188, 192
HpyCH4V TGCA 2 cut(s) 1109, 1132
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 323, 364, 373, 881
HpyF3I CTNAG 4 cut(s) 330, 678, 1064, 1103
HpySE526I ACGT 2 cut(s) 188, 192
Hsp92II CATG 5 cut(s) 280, 402, 593, 914, 989
Ksp22I TGATCA 1 cut(s) 1018
Kzo9I GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
LmnI GCTCC 1 cut(s) 1037
Lsp1109I GCAGC 2 cut(s) 854, 889
LweI GCATC 2 cut(s) 815, 1141
MaeI CTAG 3 cut(s) 231, 854, 870
MaeII ACGT 2 cut(s) 188, 192
MaeIII GTNAC 3 cut(s) 110, 209, 742
MalI GATC 6 cut(s) 122, 156, 361, 528, 649, 1020
MbiI CCGCTC 1 cut(s) 31
MboI GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
MboII GAAGA 2 cut(s) 850, 937
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 5 cut(s) 139, 269, 381, 633, 957
Mph1103I ATGCAT 1 cut(s) 1134
MroXI GAANNNNTTC 2 cut(s) 317, 570
MseI TTAA 3 cut(s) 684, 777, 1143
MslI CAYNNNNRTG 1 cut(s) 984
MspI CCGG 4 cut(s) 57, 108, 221, 475
MunI CAATTG 1 cut(s) 381
MvnI CGCG 1 cut(s) 609
MwoI GCNNNNNNNGC 5 cut(s) 28, 323, 364, 373, 881
NcoI CCATGG 1 cut(s) 276
NdeII GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
NlaIII CATG 5 cut(s) 280, 402, 593, 914, 989
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 1029
NmuCI GTSAC 3 cut(s) 110, 209, 742
NsiI ATGCAT 1 cut(s) 1134
NspI RCATGY 1 cut(s) 593
PagI TCATGA 1 cut(s) 985
PciI ACATGT 1 cut(s) 589
PdmI GAANNNNTTC 2 cut(s) 317, 570
PfeI GAWTC 4 cut(s) 234, 445, 455, 584
Pfl23II CGTACG 1 cut(s) 189
PkrI GCNGC 7 cut(s) 6, 62, 735, 844, 904, 919, 978
Ple19I CGATCG 2 cut(s) 123, 362
Ppu21I YACGTR 1 cut(s) 189
PscI ACATGT 1 cut(s) 589
PshAI GACNNNNGTC 1 cut(s) 52
PspLI CGTACG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 54, 218
PsuI RGATCY 1 cut(s) 647
PvuI CGATCG 2 cut(s) 123, 362
RsaI GTAC 2 cut(s) 191, 701
RsaNI GTAC 2 cut(s) 190, 700
RseI CAYNNNNRTG 1 cut(s) 984
Rsr2I CGGWCCG 1 cut(s) 218
RsrII CGGWCCG 1 cut(s) 218
SaqAI TTAA 3 cut(s) 684, 777, 1143
SatI GCNGC 7 cut(s) 5, 61, 734, 843, 903, 918, 977
Sau3AI GATC 6 cut(s) 120, 154, 359, 526, 647, 1018
Sau96I GGNCC 2 cut(s) 54, 218
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 2 cut(s) 815, 1141
SinI GGWCC 2 cut(s) 54, 218
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 5 cut(s) 139, 269, 381, 633, 957
SspMI CTAG 3 cut(s) 231, 854, 870
StyI CCWWGG 2 cut(s) 276, 469
TaaI ACNGT 5 cut(s) 209, 502, 516, 828, 1000
TaiI ACGT 2 cut(s) 191, 195
TaqI TCGA 4 cut(s) 17, 119, 143, 458
TasI AATT 5 cut(s) 139, 269, 381, 633, 957
TauI GCSGC 4 cut(s) 63, 736, 920, 979
TfiI GAWTC 4 cut(s) 234, 445, 455, 584
Tru1I TTAA 3 cut(s) 684, 777, 1143
Tru9I TTAA 3 cut(s) 684, 777, 1143
TscAI CASTG 4 cut(s) 328, 334, 544, 744
TseFI GTSAC 3 cut(s) 110, 209, 742
TseI GCWGC 3 cut(s) 4, 842, 902
Tsp45I GTSAC 3 cut(s) 110, 209, 742
TspDTI ATGAA 5 cut(s) 386, 563, 743, 927, 974
TspGWI ACGGA 1 cut(s) 539
TspRI CASTG 4 cut(s) 328, 334, 544, 744
VpaK11BI GGWCC 2 cut(s) 54, 218
XapI RAATTY 2 cut(s) 139, 269
XbaI TCTAGA 1 cut(s) 230
XceI RCATGY 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 2 cut(s) 317, 570
XspI CTAG 3 cut(s) 231, 854, 870
Zsp2I ATGCAT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.